hplc column  (Millipore)


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    Structured Review

    Millipore hplc column
    Hplc Column, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc column/product/Millipore
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hplc column - by Bioz Stars, 2020-02
    94/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA). ..

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: .. Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Flow Cytometry:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: The column flow rate was 2 ml/min, the mobile phase used was 42% acetonitrile/H2 O and the detector wavelength was set at 210 nm. .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA).

    Affinity Chromatography:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: .. Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Ion Exchange Chromatography:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: .. Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Modification:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: Extraction and estimation of PZQ in the different serum samples were performed using high-performance liquid chromatography (HPLC) on a series 3000 Dionex Ultimate (Dionex, Amsterdam, The Netherlands) adhering to a modification of the method described by Xiao et al. [ ]. .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA).

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. Sequencing grade modified trypsin (V5113, Promega, Madison, WI) in 50 mM acetic acid at a concentration of 0.4 μg/μl was stored at -80°C.

    Synthesized:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Nerve agent model compounds were synthesized at the Human BioMolecular Research Institute (San Diego, CA) ( ). .. Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ).

    Infection:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: Two other batches of S. mansoni- infected mice, matching the number of normal animals, followed the same procedure. .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA).

    Mouse Assay:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: Two other batches of S. mansoni- infected mice, matching the number of normal animals, followed the same procedure. .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA).

    Concentration Assay:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: For PZQ concentration estimation, 0.5 ml of sera was extracted with 3-ml aliquots of ethyl acetate and centrifuged at 1850× g for 15 min. Two-milliliter aliquots of the extracts were evaporated to dryness at 25 °C under a nitrogen stream, and the residue was then re-suspended in 200 μl of acetonitrile and shaken on a vortex mixer for 1 min. .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA).

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. Sequencing grade modified trypsin (V5113, Promega, Madison, WI) in 50 mM acetic acid at a concentration of 0.4 μg/μl was stored at -80°C.

    Activity Assay:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Protein Concentration:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Sequencing:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. Sequencing grade modified trypsin (V5113, Promega, Madison, WI) in 50 mM acetic acid at a concentration of 0.4 μg/μl was stored at -80°C.

    Injection:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: .. The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA). ..

    Purification:

    Article Title: Nerve agent analogs that produce authentic soman, sarin, tabun, and cyclohexyl methylphosphonate-modified human butyrylcholinesterase
    Article Snippet: .. Human butyrylcholinesterase was purified from outdated human plasma by ion exchange chromatography at pH 4.0 followed by affinity chromatography on procainamide-Sepharose, and anion exchange at pH 7 on a Protein-Pak DEAE 8HR 1000 Å, 10 × 100 mm HPLC column (Waters/Millipore) ( ). .. The purified butyrylcholinesterase had an activity of 540 units/mL (with 1 mM butyrylthiocholine at 25°C, pH 7.0) and a protein concentration of 0.75 mg/mL.

    Software:

    Article Title: A novel praziquantel solid lipid nanoparticle formulation shows enhanced bioavailability and antischistosomal efficacy against murine S. mansoni infection
    Article Snippet: The sample (25 µl) was then injected into a Nova Pak C18, 60 Å, 4 μm, 3.9 × 150 mm HPLC column (Millipore Co., Milford, MA, USA) equipped with a series 200 programmable multi-wavelength detector (Perkin Elmer; Norwalk, CT, USA). .. Pharm PCS software v.4.2 was used for computing pharmacokinetic parameters based on the classic method of residuals and the least square technique for curve fitting [ ].

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  • News
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  • Team
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  • 94
    Millipore hplc column
    Hplc Column, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc column/product/Millipore
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hplc column - by Bioz Stars, 2020-02
    94/100 stars
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