Structured Review

Thermo Fisher na3 vo4
Na3 Vo4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Thermo Fisher
Average 92 stars, based on 131 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

Images

Related Articles

Electrophoresis:

Article Title: Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcification. Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcificatio
Article Snippet: .. SDS‐PAGE electrophoresis and Western blotting The tissue samples were homogenized two times in five volumes (w/v) of ice cold buffer containing 50 mmol L−1 Tris HCl, (pH 7.4), 1 mmol L−1 EDTA, 150 mmol L−1 NaCl, 1 mmol L−1 NaF, 1 mmol L−1 Na3 VO4 , 1% NP‐40, 1% sodium deoxycholate, 1 mmol L−1 PMSF, and 1× HALT™ protease inhibitor cocktail (Thermo Fisher Scientific Inc.) at 50 Hz for 3 min each with 10 sec intervals using the TissueLyser LT (Qiagen GmbH). .. The protein concentration in the supernatant obtained was determined according to the method of Bradford ( ) and adjusted to 2.5 μ g μ L−1 with Laemmli buffer (Laemmli ).

Radio Immunoprecipitation:

Article Title: Potential role of autophagy in T-cell survival in polymyositis and dermatomyositis
Article Snippet: .. Western blot analysis Purified CD3+ T lymphocytes were lysed in radioimmunoprecipitation assay buffer (Thermo Fisher Scientific, Inc.) containing 100 mM Tris-HCl, pH 8; 150 mM NaCl; 1% Triton X-100; 1 mM MgCl2 ; and 25 mM Na3 VO4 and a mixture of protease inhibitors (Thermo Fisher Scientific, Inc.). ..

Protease Inhibitor:

Article Title: Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcification. Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcificatio
Article Snippet: .. SDS‐PAGE electrophoresis and Western blotting The tissue samples were homogenized two times in five volumes (w/v) of ice cold buffer containing 50 mmol L−1 Tris HCl, (pH 7.4), 1 mmol L−1 EDTA, 150 mmol L−1 NaCl, 1 mmol L−1 NaF, 1 mmol L−1 Na3 VO4 , 1% NP‐40, 1% sodium deoxycholate, 1 mmol L−1 PMSF, and 1× HALT™ protease inhibitor cocktail (Thermo Fisher Scientific Inc.) at 50 Hz for 3 min each with 10 sec intervals using the TissueLyser LT (Qiagen GmbH). .. The protein concentration in the supernatant obtained was determined according to the method of Bradford ( ) and adjusted to 2.5 μ g μ L−1 with Laemmli buffer (Laemmli ).

Article Title: A role for nucleotides in support of breast cancer angiogenesis: heterologous receptor signalling
Article Snippet: .. The medium was removed and the plates rinsed twice in ice-cold PBS containing 0.5 m Na3 VO4 and suspended in lysis buffer containing 20 m Tris HCl pH 8, 150 m NaCl, 10% glycerol, 1% Triton X-100 or Nonidet P-40 (NP-40), 1 m EGTA, 2.5 m sodium pyrophosphate, 1 m Na3 VO4 , 1 m NaF and 1% Halt protease inhibitor (Pierce Biotechnology, Rockford, IL, USA). .. Adherent cells were scraped from the plates with a plastic cell scraper, collected in centrifuge tubes and incubated at 4°C on a rocker for 15 min.

Size-exclusion Chromatography:

Article Title: Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcification. Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcificatio
Article Snippet: .. SDS‐PAGE electrophoresis and Western blotting The tissue samples were homogenized two times in five volumes (w/v) of ice cold buffer containing 50 mmol L−1 Tris HCl, (pH 7.4), 1 mmol L−1 EDTA, 150 mmol L−1 NaCl, 1 mmol L−1 NaF, 1 mmol L−1 Na3 VO4 , 1% NP‐40, 1% sodium deoxycholate, 1 mmol L−1 PMSF, and 1× HALT™ protease inhibitor cocktail (Thermo Fisher Scientific Inc.) at 50 Hz for 3 min each with 10 sec intervals using the TissueLyser LT (Qiagen GmbH). .. The protein concentration in the supernatant obtained was determined according to the method of Bradford ( ) and adjusted to 2.5 μ g μ L−1 with Laemmli buffer (Laemmli ).

Purification:

Article Title: Potential role of autophagy in T-cell survival in polymyositis and dermatomyositis
Article Snippet: .. Western blot analysis Purified CD3+ T lymphocytes were lysed in radioimmunoprecipitation assay buffer (Thermo Fisher Scientific, Inc.) containing 100 mM Tris-HCl, pH 8; 150 mM NaCl; 1% Triton X-100; 1 mM MgCl2 ; and 25 mM Na3 VO4 and a mixture of protease inhibitors (Thermo Fisher Scientific, Inc.). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Ginger Ingredients Alleviate Diabetic Prostatic Complications: Effect on Oxidative Stress and Fibrosis
Article Snippet: .. Biochemical and ELISA Measurements Tissue extraction reagent I of the following composition: 50 mM Tris, pH 7.4; 250 mM NaCl; 5 mM EDTA; 2 mM Na3 VO4 ; 1 mM NaF; 20 mM Na4 P2 O7 and 0.02% NaN3 (Invitrogen Corporation, Camarillo, CA, USA) was used to homogenize the snap frozen tissues under cooling. .. An analysis of the supernatant was carried out to determine the level of malondialdehyde and glutathione-glutathione reductase ratio in the prostate homogenate using commercially available kits (Abcam®, Cambridge, MA, USA).

Western Blot:

Article Title: Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcification. Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcificatio
Article Snippet: .. SDS‐PAGE electrophoresis and Western blotting The tissue samples were homogenized two times in five volumes (w/v) of ice cold buffer containing 50 mmol L−1 Tris HCl, (pH 7.4), 1 mmol L−1 EDTA, 150 mmol L−1 NaCl, 1 mmol L−1 NaF, 1 mmol L−1 Na3 VO4 , 1% NP‐40, 1% sodium deoxycholate, 1 mmol L−1 PMSF, and 1× HALT™ protease inhibitor cocktail (Thermo Fisher Scientific Inc.) at 50 Hz for 3 min each with 10 sec intervals using the TissueLyser LT (Qiagen GmbH). .. The protein concentration in the supernatant obtained was determined according to the method of Bradford ( ) and adjusted to 2.5 μ g μ L−1 with Laemmli buffer (Laemmli ).

Article Title: Evaluation and Optimization of the Anti-Melanogenic Activity of 1-(2-Cyclohexylmethoxy-6-hydroxy-phenyl)-3-(4-hydroxymethyl-phenyl)-propenone Derivatives
Article Snippet: .. Western Blot Analysis Whole-cell lysates were prepared on ice for 30 min using a lysis buffer containing 50 mM Tris-HCl (pH 7.4), 350 mM NaCl, 1% Triton X-100, 0.5% Nonidet P-40, 10% glycerol, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3 VO4 , 1 mM PMSF, and phosphatase inhibitor cocktail (Thermo Scientific, Pittsburgh, PA, USA). .. The lysates were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO, USA).

Article Title: Potential role of autophagy in T-cell survival in polymyositis and dermatomyositis
Article Snippet: .. Western blot analysis Purified CD3+ T lymphocytes were lysed in radioimmunoprecipitation assay buffer (Thermo Fisher Scientific, Inc.) containing 100 mM Tris-HCl, pH 8; 150 mM NaCl; 1% Triton X-100; 1 mM MgCl2 ; and 25 mM Na3 VO4 and a mixture of protease inhibitors (Thermo Fisher Scientific, Inc.). ..

Modification:

Article Title: IFNγ-Dependent Tissue-Immune Homeostasis Is Co-opted in the Tumor Microenvironment
Article Snippet: .. Cells were lysed in modified RIPA buffer containing 50 mM Tris (pH 7.4), 1% NP-40,150 mM NaCl, 1 mM EDTA, 1 mM Na3 VO4 , and protease and phosphatase inhibitor cocktail (Life Technologies). .. Protein lysates were quantified using protein assay dye reagent (Bio-Rad).

Article Title: Inhibition of IgE-mediated Secretion from Human Basophils with a Highly Selective Bruton’s Tyrosine Kinase, Btk, Inhibitor
Article Snippet: .. The following were purchased: PIPES, bovine serum albumin (BSA), EGTA, EDTA, D-glucose, NaF, Na3 VO4 , 2-ME (2-mercaptoethanol); RPMI 1640 containing 25 mM HEPES and L-glutamine (BioWhittaker, Walkersville, MD); IMDM, Iscove’s modified Dulbecco’s Medium (Gibco/Invitrogen, Carlsbad, CA); Percoll (Pharmacia, Piscataway, NJ); Tris(hydroxymethyl)-aminomethane, Tween-20 (Bio-Rad,Hercules, CA); anti-syk mAb(4D10) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-p85 (Upstate Biotechnology, Charlottesville, VA); anti-btk, (BD Biosciences, San Jose, CA); HRP-conjugated sheep anti-mouse Ig Ab (Amersham Life Science, Arlington Heights, IL). ..

Lysis:

Article Title: A role for nucleotides in support of breast cancer angiogenesis: heterologous receptor signalling
Article Snippet: .. The medium was removed and the plates rinsed twice in ice-cold PBS containing 0.5 m Na3 VO4 and suspended in lysis buffer containing 20 m Tris HCl pH 8, 150 m NaCl, 10% glycerol, 1% Triton X-100 or Nonidet P-40 (NP-40), 1 m EGTA, 2.5 m sodium pyrophosphate, 1 m Na3 VO4 , 1 m NaF and 1% Halt protease inhibitor (Pierce Biotechnology, Rockford, IL, USA). .. Adherent cells were scraped from the plates with a plastic cell scraper, collected in centrifuge tubes and incubated at 4°C on a rocker for 15 min.

Article Title: Evaluation and Optimization of the Anti-Melanogenic Activity of 1-(2-Cyclohexylmethoxy-6-hydroxy-phenyl)-3-(4-hydroxymethyl-phenyl)-propenone Derivatives
Article Snippet: .. Western Blot Analysis Whole-cell lysates were prepared on ice for 30 min using a lysis buffer containing 50 mM Tris-HCl (pH 7.4), 350 mM NaCl, 1% Triton X-100, 0.5% Nonidet P-40, 10% glycerol, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3 VO4 , 1 mM PMSF, and phosphatase inhibitor cocktail (Thermo Scientific, Pittsburgh, PA, USA). .. The lysates were resolved by SDS-PAGE and transferred onto nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO, USA).

Article Title: Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases
Article Snippet: .. Total protein was extracted with lysis buffer containing 2 mM CaCl2 , 1 mM Na3 VO4 , and 1× phosphatase inhibitor (1862495; Thermo Fisher Scientific) and immunoblotted with anti–pY731-VEC (441145G; Invitrogen; or rat anti-mouse pY731-VEC monoclonal [pY731-73.1], a gift from D. Vestweber; ). ..

SDS Page:

Article Title: Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcification. Light‐dependent expression of a Na+/H+ exchanger 3‐like transporter in the ctenidium of the giant clam, Tridacna squamosa, can be related to increased H+ excretion during light‐enhanced calcificatio
Article Snippet: .. SDS‐PAGE electrophoresis and Western blotting The tissue samples were homogenized two times in five volumes (w/v) of ice cold buffer containing 50 mmol L−1 Tris HCl, (pH 7.4), 1 mmol L−1 EDTA, 150 mmol L−1 NaCl, 1 mmol L−1 NaF, 1 mmol L−1 Na3 VO4 , 1% NP‐40, 1% sodium deoxycholate, 1 mmol L−1 PMSF, and 1× HALT™ protease inhibitor cocktail (Thermo Fisher Scientific Inc.) at 50 Hz for 3 min each with 10 sec intervals using the TissueLyser LT (Qiagen GmbH). .. The protein concentration in the supernatant obtained was determined according to the method of Bradford ( ) and adjusted to 2.5 μ g μ L−1 with Laemmli buffer (Laemmli ).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Thermo Fisher na3 vo4
    Na3 Vo4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Thermo Fisher
    Average 92 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Thermo Fisher m na3 vo4
    M Na3 Vo4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Thermo Fisher
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Thermo Fisher sodium vanadate
    Sodium Vanadate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium vanadate/product/Thermo Fisher
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    sodium vanadate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results