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Nacalai na3 vo4
Na3 Vo4, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Nacalai
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Protease Inhibitor:

Article Title: Anks4b, a Novel Target of HNF4? Protein, Interacts with GRP78 Protein and Regulates Endoplasmic Reticulum Stress-induced Apoptosis in Pancreatic ?-Cells *
Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 0.1% SDS, 1% Nonidet P-40, 5 m m EDTA, 0.5% sodium deoxycholate, 20 μg/ml Na3 VO4 , 10 m m NaF, 1 m m PMSF, 2 m m DTT, and protease inhibitor mixture (1/100)) from Nacalai Tesque. .. Total protein was separated by SDS-polyacrylamide gel electrophoresis, transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA), and probed with primary antibodies.

Article Title: Enhancing Beta-Catenin Activity via GSK3beta Inhibition Protects PC12 Cells against Rotenone Toxicity through Nurr1 Induction
Article Snippet: .. Briefly, cells were lysed in ice-cold immunoprecipitation (IP) buffer containing 50 mmol/L Tris-HCl (pH 8.0), 150 mmol/L NaCl, 1% NP-40, 1 mmol/L Na3 VO4 , and protease inhibitor cocktail (Nacalai Tesque). .. The supernatant was pre-cleared with protein-A-Sepharose beads and incubated overnight at 4°C.

Article Title: Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis
Article Snippet: .. Western blot analysis Mouse skin tissue was homogenized in RIPA buffer (1% NP-40, 50 mM Tris-HCl (pH7.5), 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 5 mM NaF, 1 mM EDTA, 1 mM Na3 VO4 , plus a protease inhibitor cocktail [Nacalai Tesque, Kyoto Japan]). .. Total protein (10 μg) was separated by SDS-PAGE and transferred to PVDF membranes.

Article Title: High-Molecular-Weight Polyphenol-Rich Fraction of Black Tea Does Not Prevent Atrophy by Unloading, But Promotes Soleus Muscle Mass Recovery from Atrophy in Mice
Article Snippet: .. Western Blot Analysis To prepare total protein lysate, frozen muscle samples were homogenized in lysis buffer (1% Nonidet-P40, 1% sodium deoxycholate, 0.2% sodium dodecyl sulfate(SDS), 150 mM NaCl, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (pH = 7.5), 10 mM ethylenediaminetetraacetic acid (EDTA), 10 mM NaF, 10 mM Na4 P2 O7 , and 2 mM Na3 VO4 ) supplemented with 1% protease inhibitor cocktail for mammalian tissues (No. 162-0177; Nacalai Tesque, Kyoto, Japan). .. The concentration of protein was determined by the bicinchoninic acid method using the Protein Assay Bicinchoninate Kit (No. 297-73101, Wako, Osaka, Japan).

Article Title: An Artificial Reaction Promoter Modulates Mitochondrial Functions via Chemically Promoting Protein Acetylation
Article Snippet: .. The samples were lysed in RIPA buffer containing 25 mM HEPES, 1.5% TritonX-100 (v/v), 1.0% sodium-deoxycholate (w/v), 0.1% SDS (w/v), 500 mM NaCl, 5 mM EDTA, 50 mM NaF, 100 μM Na3 VO4 , and 0.1 mg/mL leupeptin and protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). .. The protein lysates were diluted to the same protein concentrations, separated using SDS-PAGE, transferred onto a PVDF membrane (Millipore, Billerica, MA, USA), and probed with an acetylated lysine-specific antibody (Sigma-Aldrich, St. Louis, MO, USA).

Article Title: CFC1 is a cancer stemness-regulating factor in neuroblastoma
Article Snippet: .. Western blot analysis Cells were lysed in buffer containing 5 mM EDTA, 2 mM Tris-HCl (pH 7.5), 10 mM β-glycerophosphate, 5 μg/ml aprotinin, 2 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan), and 1% SDS. ..

Article Title: Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation
Article Snippet: .. Protein lysates were obtained by homogenizing HUVECs, SMCs, or tissue samples with Triton-based lysis buffer containing 1% Triton X-100, 20 mmol/l Tris (pH 7.4), 150 mmol/l NaCl, 1 mM EDTA, 1 mmol/l EGTA, 2.5 mmol/l sodium pyrophosphate, 1 mmol/l β-glycerol phosphate, 1 mmol/l NA3 VO4 , 1 μg/ml leupeptin, and 0.1% protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). .. Protein concentration was determined using a bicinchoninic acid method (Pierce, Rockford, IL).

Article Title: Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic ?-cells
Article Snippet: .. Then, plasma membrane fractions were obtained by the method described by Nishiumi and Ashida. Briefly, to prepare the plasma membrane and post-plasma membrane fraction, L6 myotubes were harvested with buffer A (50 mM Tris-HCl (pH 8.0), 0.5 mM DTT, protease inhibitor cocktail and 1 mM Na3 VO4 (Nacalai Tesque, Inc., Kyoto, Japan) containing 0.1% (v/v) IGEPAL CA-630 (Sigma Chemical Co.), and homogenated with 21-gauge needle. ..

Immunoprecipitation:

Article Title: Enhancing Beta-Catenin Activity via GSK3beta Inhibition Protects PC12 Cells against Rotenone Toxicity through Nurr1 Induction
Article Snippet: .. Briefly, cells were lysed in ice-cold immunoprecipitation (IP) buffer containing 50 mmol/L Tris-HCl (pH 8.0), 150 mmol/L NaCl, 1% NP-40, 1 mmol/L Na3 VO4 , and protease inhibitor cocktail (Nacalai Tesque). .. The supernatant was pre-cleared with protein-A-Sepharose beads and incubated overnight at 4°C.

Western Blot:

Article Title: Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis
Article Snippet: .. Western blot analysis Mouse skin tissue was homogenized in RIPA buffer (1% NP-40, 50 mM Tris-HCl (pH7.5), 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 5 mM NaF, 1 mM EDTA, 1 mM Na3 VO4 , plus a protease inhibitor cocktail [Nacalai Tesque, Kyoto Japan]). .. Total protein (10 μg) was separated by SDS-PAGE and transferred to PVDF membranes.

Article Title: High-Molecular-Weight Polyphenol-Rich Fraction of Black Tea Does Not Prevent Atrophy by Unloading, But Promotes Soleus Muscle Mass Recovery from Atrophy in Mice
Article Snippet: .. Western Blot Analysis To prepare total protein lysate, frozen muscle samples were homogenized in lysis buffer (1% Nonidet-P40, 1% sodium deoxycholate, 0.2% sodium dodecyl sulfate(SDS), 150 mM NaCl, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (pH = 7.5), 10 mM ethylenediaminetetraacetic acid (EDTA), 10 mM NaF, 10 mM Na4 P2 O7 , and 2 mM Na3 VO4 ) supplemented with 1% protease inhibitor cocktail for mammalian tissues (No. 162-0177; Nacalai Tesque, Kyoto, Japan). .. The concentration of protein was determined by the bicinchoninic acid method using the Protein Assay Bicinchoninate Kit (No. 297-73101, Wako, Osaka, Japan).

Article Title: CFC1 is a cancer stemness-regulating factor in neuroblastoma
Article Snippet: .. Western blot analysis Cells were lysed in buffer containing 5 mM EDTA, 2 mM Tris-HCl (pH 7.5), 10 mM β-glycerophosphate, 5 μg/ml aprotinin, 2 mM phenylmethylsulfonyl fluoride, 1 mM Na3 VO4 , protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan), and 1% SDS. ..

Radio Immunoprecipitation:

Article Title: Anks4b, a Novel Target of HNF4? Protein, Interacts with GRP78 Protein and Regulates Endoplasmic Reticulum Stress-induced Apoptosis in Pancreatic ?-Cells *
Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 0.1% SDS, 1% Nonidet P-40, 5 m m EDTA, 0.5% sodium deoxycholate, 20 μg/ml Na3 VO4 , 10 m m NaF, 1 m m PMSF, 2 m m DTT, and protease inhibitor mixture (1/100)) from Nacalai Tesque. .. Total protein was separated by SDS-polyacrylamide gel electrophoresis, transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA), and probed with primary antibodies.

Lysis:

Article Title: High-Molecular-Weight Polyphenol-Rich Fraction of Black Tea Does Not Prevent Atrophy by Unloading, But Promotes Soleus Muscle Mass Recovery from Atrophy in Mice
Article Snippet: .. Western Blot Analysis To prepare total protein lysate, frozen muscle samples were homogenized in lysis buffer (1% Nonidet-P40, 1% sodium deoxycholate, 0.2% sodium dodecyl sulfate(SDS), 150 mM NaCl, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (pH = 7.5), 10 mM ethylenediaminetetraacetic acid (EDTA), 10 mM NaF, 10 mM Na4 P2 O7 , and 2 mM Na3 VO4 ) supplemented with 1% protease inhibitor cocktail for mammalian tissues (No. 162-0177; Nacalai Tesque, Kyoto, Japan). .. The concentration of protein was determined by the bicinchoninic acid method using the Protein Assay Bicinchoninate Kit (No. 297-73101, Wako, Osaka, Japan).

Article Title: Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation
Article Snippet: .. Protein lysates were obtained by homogenizing HUVECs, SMCs, or tissue samples with Triton-based lysis buffer containing 1% Triton X-100, 20 mmol/l Tris (pH 7.4), 150 mmol/l NaCl, 1 mM EDTA, 1 mmol/l EGTA, 2.5 mmol/l sodium pyrophosphate, 1 mmol/l β-glycerol phosphate, 1 mmol/l NA3 VO4 , 1 μg/ml leupeptin, and 0.1% protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). .. Protein concentration was determined using a bicinchoninic acid method (Pierce, Rockford, IL).

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    Nacalai na3 vo4
    Na3 Vo4, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Nacalai
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
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