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Merck & Co na3 vo4
Na3 Vo4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Merck & Co
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Modification:

Article Title: Defects in G-Actin Incorporation into Filaments in Myoblasts Derived from Dysferlinopathy Patients Are Restored by Dysferlin C2 Domains
Article Snippet: .. Reagents Actin, from rabbit muscle, Alexa Fluor™ 488 conjugate (Thermo Fisher Scientific, Waltham, MA, USA); ATP (Sigma-Aldrich, St. Louis, MO, USA); bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA); dexamethasone (Sigma-Aldrich, St. Louis, MO, USA); 40,6-diamidino-2-phenylindole (Sigma-Aldrich, St. Louis, MO, USA); digitonin (Sigma-Aldrich St. Louis, MO, USA); dimethyl sulfoxide (Merck Company, Darmstadt, Germany); DMEM/F-12 medium (Gibco, BRL, Gaithersburg, MD, USA); Dulbecco modified Eagle’s minimal essential medium (Gibco BRL, Gaithersburg, MD, USA); EDTA (Calbiochem, La Jolla, CA); EGTA (Sigma-Aldrich, St. Louis, MO, USA); fetal bovine serum (Gibco BRL, Gaithersburg, MD, USA); fetuin (Sigma-Aldrich, St. Louis, MO, USA); gentamicin (Gibco/Life Technology, China); glutamic acid (Sigma-Aldrich, St. Louis, MO, USA); HEPES (Calbiochem, La Jolla, CA); human insulin (Eli Lilly and company, Indianapolis, USA); Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA); 199 medium (Sigma-Aldrich St. Louis, MO, USA); NaF (Merck Company, Darmstadt, Germany); Na3 VO4 (Merck Company, Darmstadt, Germany); Opti-Mem (Gibco BRL, Gaithersburg, MD, USA); penicillin (OPKO, Santiago, Chile); p-formaldehyde (Sigma-Aldrich St. Louis, MO, USA); phenylmethyl sulfonylflouride (Sigma-Aldrich, St. Louis, MO, USA); PIPES (Sigma-Aldrich, St. Louis, MO, USA); poly-l-lysine (Sigma-Aldrich, St. Louis, MO, USA); recombinant human basic fibroblast growth factor (Gibco BRL, Gaithersburg, MD, USA); protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA); recombinant human epidermal growth factor (Gibco BRL, Gaithersburg, MD, USA); triton X-100 (Merck Company, Darmstadt, Germany); trypsin- EDTA 0.25% (Sigma-Aldrich, St. Louis, MO, USA); and Tween-20 (Merck Company, Darmstadt, Germany) were purchased. .. Antibodies Anti-annexin A2 (Santa Cruz Biotechnology, Inc, Dallas, Texas, USA); anti-β-tubulin (Cytoskeleton, St. Denver, CO, USA); anti-dysferlin-HAMLET (Novocastra TM Lyophilized Leica; Newcastle, United Kingdom); CY2-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); Cy3-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); horseradish peroxidase (HRP)-conjugated donkey anti-sheep (R & D Systems, Minneapolis, USA); HRP-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA); and peroxidase affiniPure F(ab’)2 fragment donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA) were purchased.

Protease Inhibitor:

Article Title: FASN Is a Biomarker Enriched in Malignant Glioma-Derived Extracellular Vesicles
Article Snippet: .. Immunoblot Analysis Cells and EVs were lysed using RIPA buffer, containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, aprotinin (10 mg/mL), 1 mM phenylmethylsulfonyl fluoride, leupeptin (10 mg/mL), 2 mM Na3 VO4 , 4 mM EDTA, 10 mM NaF, 10 mM sodium pyrophosphate, 1% NP-40, 0.1% sodium deoxycholate and 1% protease inhibitor (Merck). .. Total protein concentration was measured using the BCA assay.

Article Title: Lyn controls chemotaxis and motility of CLL cells via phosphorylation of ROR1
Article Snippet: .. Prior to lysis, the buffer was supplemented with 1mM Na3 VO4 , 1mM DTT, 1mM NaF and with cOmplete™ protease inhibitor cocktail and phosphatase inhibitor cocktail set II (Merck, Kenilworth, NJ). .. For the western blotting, samples were loaded on 8% gels and separated by SDS-PAGE followed by the transfer done on to Immobilon-P® (Merck) PVDF membranes at 106 V for 75 minutes.

Article Title: ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer’s disease
Article Snippet: .. PBS-washed cells were resuspended in a lysis buffer containing 1% Triton X-100, 50 mM Tris⋅HCl (pH 7.5), 150 mM NaCl, 25 μM β-glycerophosphate, 1 mM Na3 VO4 , and protease inhibitor mixture set V (Merck). .. Following the removal of cell debris by centrifugation, clarified lysates were processed for immunoprecipitation, Western blotting, or quantification of protein content.

Article Title: ADAMTS-1 and syndecan-4 intersect in the regulation of cell migration and angiogenesis
Article Snippet: .. Cells were lysed in 200 mM NaCl, 75 mM Tris, 15 mM NaF, 1.5 mM Na3 VO4 , 7.5 mM EDTA, 7.5 mM EGTA and 1.5% Triton X-100, supplemented with protease inhibitor (Merck). .. Lysates were cleared by centrifugation at 10,000 g for 10 min. Levels of syndecan-4 internalisation were determined by capture ELISA.

Article Title: Defects in G-Actin Incorporation into Filaments in Myoblasts Derived from Dysferlinopathy Patients Are Restored by Dysferlin C2 Domains
Article Snippet: .. Reagents Actin, from rabbit muscle, Alexa Fluor™ 488 conjugate (Thermo Fisher Scientific, Waltham, MA, USA); ATP (Sigma-Aldrich, St. Louis, MO, USA); bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA); dexamethasone (Sigma-Aldrich, St. Louis, MO, USA); 40,6-diamidino-2-phenylindole (Sigma-Aldrich, St. Louis, MO, USA); digitonin (Sigma-Aldrich St. Louis, MO, USA); dimethyl sulfoxide (Merck Company, Darmstadt, Germany); DMEM/F-12 medium (Gibco, BRL, Gaithersburg, MD, USA); Dulbecco modified Eagle’s minimal essential medium (Gibco BRL, Gaithersburg, MD, USA); EDTA (Calbiochem, La Jolla, CA); EGTA (Sigma-Aldrich, St. Louis, MO, USA); fetal bovine serum (Gibco BRL, Gaithersburg, MD, USA); fetuin (Sigma-Aldrich, St. Louis, MO, USA); gentamicin (Gibco/Life Technology, China); glutamic acid (Sigma-Aldrich, St. Louis, MO, USA); HEPES (Calbiochem, La Jolla, CA); human insulin (Eli Lilly and company, Indianapolis, USA); Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA); 199 medium (Sigma-Aldrich St. Louis, MO, USA); NaF (Merck Company, Darmstadt, Germany); Na3 VO4 (Merck Company, Darmstadt, Germany); Opti-Mem (Gibco BRL, Gaithersburg, MD, USA); penicillin (OPKO, Santiago, Chile); p-formaldehyde (Sigma-Aldrich St. Louis, MO, USA); phenylmethyl sulfonylflouride (Sigma-Aldrich, St. Louis, MO, USA); PIPES (Sigma-Aldrich, St. Louis, MO, USA); poly-l-lysine (Sigma-Aldrich, St. Louis, MO, USA); recombinant human basic fibroblast growth factor (Gibco BRL, Gaithersburg, MD, USA); protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA); recombinant human epidermal growth factor (Gibco BRL, Gaithersburg, MD, USA); triton X-100 (Merck Company, Darmstadt, Germany); trypsin- EDTA 0.25% (Sigma-Aldrich, St. Louis, MO, USA); and Tween-20 (Merck Company, Darmstadt, Germany) were purchased. .. Antibodies Anti-annexin A2 (Santa Cruz Biotechnology, Inc, Dallas, Texas, USA); anti-β-tubulin (Cytoskeleton, St. Denver, CO, USA); anti-dysferlin-HAMLET (Novocastra TM Lyophilized Leica; Newcastle, United Kingdom); CY2-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); Cy3-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); horseradish peroxidase (HRP)-conjugated donkey anti-sheep (R & D Systems, Minneapolis, USA); HRP-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA); and peroxidase affiniPure F(ab’)2 fragment donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA) were purchased.

Recombinant:

Article Title: Defects in G-Actin Incorporation into Filaments in Myoblasts Derived from Dysferlinopathy Patients Are Restored by Dysferlin C2 Domains
Article Snippet: .. Reagents Actin, from rabbit muscle, Alexa Fluor™ 488 conjugate (Thermo Fisher Scientific, Waltham, MA, USA); ATP (Sigma-Aldrich, St. Louis, MO, USA); bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA); dexamethasone (Sigma-Aldrich, St. Louis, MO, USA); 40,6-diamidino-2-phenylindole (Sigma-Aldrich, St. Louis, MO, USA); digitonin (Sigma-Aldrich St. Louis, MO, USA); dimethyl sulfoxide (Merck Company, Darmstadt, Germany); DMEM/F-12 medium (Gibco, BRL, Gaithersburg, MD, USA); Dulbecco modified Eagle’s minimal essential medium (Gibco BRL, Gaithersburg, MD, USA); EDTA (Calbiochem, La Jolla, CA); EGTA (Sigma-Aldrich, St. Louis, MO, USA); fetal bovine serum (Gibco BRL, Gaithersburg, MD, USA); fetuin (Sigma-Aldrich, St. Louis, MO, USA); gentamicin (Gibco/Life Technology, China); glutamic acid (Sigma-Aldrich, St. Louis, MO, USA); HEPES (Calbiochem, La Jolla, CA); human insulin (Eli Lilly and company, Indianapolis, USA); Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA); 199 medium (Sigma-Aldrich St. Louis, MO, USA); NaF (Merck Company, Darmstadt, Germany); Na3 VO4 (Merck Company, Darmstadt, Germany); Opti-Mem (Gibco BRL, Gaithersburg, MD, USA); penicillin (OPKO, Santiago, Chile); p-formaldehyde (Sigma-Aldrich St. Louis, MO, USA); phenylmethyl sulfonylflouride (Sigma-Aldrich, St. Louis, MO, USA); PIPES (Sigma-Aldrich, St. Louis, MO, USA); poly-l-lysine (Sigma-Aldrich, St. Louis, MO, USA); recombinant human basic fibroblast growth factor (Gibco BRL, Gaithersburg, MD, USA); protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA); recombinant human epidermal growth factor (Gibco BRL, Gaithersburg, MD, USA); triton X-100 (Merck Company, Darmstadt, Germany); trypsin- EDTA 0.25% (Sigma-Aldrich, St. Louis, MO, USA); and Tween-20 (Merck Company, Darmstadt, Germany) were purchased. .. Antibodies Anti-annexin A2 (Santa Cruz Biotechnology, Inc, Dallas, Texas, USA); anti-β-tubulin (Cytoskeleton, St. Denver, CO, USA); anti-dysferlin-HAMLET (Novocastra TM Lyophilized Leica; Newcastle, United Kingdom); CY2-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); Cy3-conjugated goat anti-rabbit IgG (H+L) (Jackson Immunoresearch, West Grove, PA, USA); horseradish peroxidase (HRP)-conjugated donkey anti-sheep (R & D Systems, Minneapolis, USA); HRP-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher Scientific, Waltham, MA, USA); and peroxidase affiniPure F(ab’)2 fragment donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch, West Grove, PA, USA) were purchased.

Lysis:

Article Title: Lyn controls chemotaxis and motility of CLL cells via phosphorylation of ROR1
Article Snippet: .. Prior to lysis, the buffer was supplemented with 1mM Na3 VO4 , 1mM DTT, 1mM NaF and with cOmplete™ protease inhibitor cocktail and phosphatase inhibitor cocktail set II (Merck, Kenilworth, NJ). .. For the western blotting, samples were loaded on 8% gels and separated by SDS-PAGE followed by the transfer done on to Immobilon-P® (Merck) PVDF membranes at 106 V for 75 minutes.

Article Title: ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer’s disease
Article Snippet: .. PBS-washed cells were resuspended in a lysis buffer containing 1% Triton X-100, 50 mM Tris⋅HCl (pH 7.5), 150 mM NaCl, 25 μM β-glycerophosphate, 1 mM Na3 VO4 , and protease inhibitor mixture set V (Merck). .. Following the removal of cell debris by centrifugation, clarified lysates were processed for immunoprecipitation, Western blotting, or quantification of protein content.

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    Merck & Co phosphatase inhibitor cocktail
    Phosphatase Inhibitor Cocktail, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Merck & Co
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail - by Bioz Stars, 2020-07
    93/100 stars
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    92
    Merck & Co na3 vo4
    Na3 Vo4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Merck & Co
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Merck & Co m na3 vo4
    M Na3 Vo4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Merck & Co
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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