Structured Review

Merck & Co na3 vo4
Na3 Vo4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2021-07
86/100 stars

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Western Blot:

Article Title: Zearalenone Induces Endothelial Cell Apoptosis through Activation of a Cytosolic Ca2+/ERK1/2/p53/Caspase 3 Signaling Pathway
Article Snippet: The BAECs with or without transfections were grown to confluence and maintained for an additional 24 h in MEM with 1% NCS containing 30 μM ZEN. .. Western Blot Analyses For the western blot analyses, BAECs treated with ZEN in the absence or presence of various additional chemicals were washed with ice-cold Dulbecco’s phosphate-buffered saline (DPBS) and lysed with lysis buffer (20 mM Tris-HCl at pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA) containing Protease Inhibitor Cocktail™ (Merck), 1 mM β-glycerophosphate, 1 mM phenylmethanesulfonyl fluoride, 1 mM NaF and 1 mM Na3 VO4. .. The protein concentrations were determined using a BCA protein assay (Merck).

Article Title: Depletion of intermediate filament protein Nestin, a target of microRNA-940, suppresses tumorigenesis by inducing spontaneous DNA damage accumulation in human nasopharyngeal carcinoma
Article Snippet: The staining was visualized by adding BM purple AP substrate (Roche Applied Science) according to the manufacturer's instructions. .. Western blotting After transfection and/or treatment, cells were subjected to western blotting with anti-Nestin (1 : 500; Merck), anti-γ H2AX (1 : 1000; Cell Signaling, Beverly, MA, USA) or β -actin (1 : 1500; Santa Cruz Biotechnology Inc, Dallas, TX, USA) antibodies. .. Cells were harvested and lysed in 0.5 ml of lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM EDTA, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM NaF, 0.1 mM Na3 VO4 , 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and protease inhibitor cocktail (Roche Applied Science).

Lysis:

Article Title: Zearalenone Induces Endothelial Cell Apoptosis through Activation of a Cytosolic Ca2+/ERK1/2/p53/Caspase 3 Signaling Pathway
Article Snippet: The BAECs with or without transfections were grown to confluence and maintained for an additional 24 h in MEM with 1% NCS containing 30 μM ZEN. .. Western Blot Analyses For the western blot analyses, BAECs treated with ZEN in the absence or presence of various additional chemicals were washed with ice-cold Dulbecco’s phosphate-buffered saline (DPBS) and lysed with lysis buffer (20 mM Tris-HCl at pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA) containing Protease Inhibitor Cocktail™ (Merck), 1 mM β-glycerophosphate, 1 mM phenylmethanesulfonyl fluoride, 1 mM NaF and 1 mM Na3 VO4. .. The protein concentrations were determined using a BCA protein assay (Merck).

Article Title: A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases
Article Snippet: After 40 h, cells were lysed and subjected to coimmunoprecipitation and Western blotting. .. Coimmunoprecipitation Transfected cells were lysed using cold mammalian lysis buffer [50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM Na3 VO4 , 1 mM β-glycerophosphate disodium salt hydrate, 1× mammalian protease inhibitor complex (Sigma), and 1% Triton X-100] and pelleted at 13 000g for 20 min. RhoGDIs were precipitated with protein G beads (Merck) conjugated to 1 mg/mL anti-FLAG (ThermoFisher Scientific) or nickel-coated beads (Merck) according to the manufacturer’s instruction. .. Immunoprecipitated proteins were then eluted in NuPAGE LDS sample buffer (ThermoFisher Scientific) containing 2.3 M β-mercaptoethanol.

Protease Inhibitor:

Article Title: Zearalenone Induces Endothelial Cell Apoptosis through Activation of a Cytosolic Ca2+/ERK1/2/p53/Caspase 3 Signaling Pathway
Article Snippet: The BAECs with or without transfections were grown to confluence and maintained for an additional 24 h in MEM with 1% NCS containing 30 μM ZEN. .. Western Blot Analyses For the western blot analyses, BAECs treated with ZEN in the absence or presence of various additional chemicals were washed with ice-cold Dulbecco’s phosphate-buffered saline (DPBS) and lysed with lysis buffer (20 mM Tris-HCl at pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA) containing Protease Inhibitor Cocktail™ (Merck), 1 mM β-glycerophosphate, 1 mM phenylmethanesulfonyl fluoride, 1 mM NaF and 1 mM Na3 VO4. .. The protein concentrations were determined using a BCA protein assay (Merck).

Article Title: A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases
Article Snippet: After 40 h, cells were lysed and subjected to coimmunoprecipitation and Western blotting. .. Coimmunoprecipitation Transfected cells were lysed using cold mammalian lysis buffer [50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM Na3 VO4 , 1 mM β-glycerophosphate disodium salt hydrate, 1× mammalian protease inhibitor complex (Sigma), and 1% Triton X-100] and pelleted at 13 000g for 20 min. RhoGDIs were precipitated with protein G beads (Merck) conjugated to 1 mg/mL anti-FLAG (ThermoFisher Scientific) or nickel-coated beads (Merck) according to the manufacturer’s instruction. .. Immunoprecipitated proteins were then eluted in NuPAGE LDS sample buffer (ThermoFisher Scientific) containing 2.3 M β-mercaptoethanol.

Transfection:

Article Title: Depletion of intermediate filament protein Nestin, a target of microRNA-940, suppresses tumorigenesis by inducing spontaneous DNA damage accumulation in human nasopharyngeal carcinoma
Article Snippet: The staining was visualized by adding BM purple AP substrate (Roche Applied Science) according to the manufacturer's instructions. .. Western blotting After transfection and/or treatment, cells were subjected to western blotting with anti-Nestin (1 : 500; Merck), anti-γ H2AX (1 : 1000; Cell Signaling, Beverly, MA, USA) or β -actin (1 : 1500; Santa Cruz Biotechnology Inc, Dallas, TX, USA) antibodies. .. Cells were harvested and lysed in 0.5 ml of lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM EDTA, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM NaF, 0.1 mM Na3 VO4 , 1% Triton X-100, 1 mM phenylmethylsulfonyl fluoride and protease inhibitor cocktail (Roche Applied Science).

Article Title: A Complete Survey of RhoGDI Targets Reveals Novel Interactions with Atypical Small GTPases
Article Snippet: After 40 h, cells were lysed and subjected to coimmunoprecipitation and Western blotting. .. Coimmunoprecipitation Transfected cells were lysed using cold mammalian lysis buffer [50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM Na3 VO4 , 1 mM β-glycerophosphate disodium salt hydrate, 1× mammalian protease inhibitor complex (Sigma), and 1% Triton X-100] and pelleted at 13 000g for 20 min. RhoGDIs were precipitated with protein G beads (Merck) conjugated to 1 mg/mL anti-FLAG (ThermoFisher Scientific) or nickel-coated beads (Merck) according to the manufacturer’s instruction. .. Immunoprecipitated proteins were then eluted in NuPAGE LDS sample buffer (ThermoFisher Scientific) containing 2.3 M β-mercaptoethanol.

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    Merck & Co phosphatase inhibitor cocktail
    Phosphatase Inhibitor Cocktail, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co na3 vo4
    Na3 Vo4, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co phosphatase inhibitors
    Phosphatase Inhibitors, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitors/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitors - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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