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Fisher Scientific na3 vo4
Na3 Vo4, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Fisher Scientific
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Protease Inhibitor:

Article Title: Blockade of Protein Geranylgeranylation Inhibits Cdk2-Dependent p27Kip1 Phosphorylation on Thr187 and Accumulates p27Kip1 in the Nucleus: Implications for Breast Cancer Therapy ▿ in the Nucleus: Implications for Breast Cancer Therapy ▿ §
Article Snippet: .. Weighted tumor samples were mixed with the appropriate amount of the T-PER tissue protein extraction reagent (Pierce, Rockford, IL) containing protease inhibitor cocktail, 2 mM PMSF, 2 mM Na3 VO4 , and 6.4 mg/ml p -nitrophenylphosphate and homogenized with the PCR tissue homogenizing kit (Fisher Scientific). ..

Article Title: All-trans retinoic acid increases the expression of oxidative myosin heavy chain through the PPARδ pathway in bovine muscle cells derived from satellite cells
Article Snippet: .. At 96 h of treatment, cultured bovine satellite cells were isolated using 200 μL of iced buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ), protease inhibitor (Roche, Switzerland), and 2 mM Na3 VO4 (Fisher Scientific). .. Samples were diluted with PBS to determine concentration via Pierce BCA protein assay (Thermo Fisher Science).

Article Title: The transient expression of Klf4 and Klf5 during adipogenesis depends on GSK3β activity
Article Snippet: .. Baker, 8993), 2.5 mM sodium pyrophosphate (Sigma, 221368), 100 mM β-glycerolphosphate(Sigma, 50020), 1 mM Na3 VO4 (Fisher Scientific, S-454), and Complete protease inhibitor cocktail 2X (Roche, 1697498). ..

Cell Culture:

Article Title: Chromium Propionate Enhances Adipogenic Differentiation of Bovine Intramuscular Adipocytes
Article Snippet: .. Western blotting Protein from cultured BSC and preadipocytes (IM and SC) was isolated with 200 μL of ice-cold buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ, USA), protein inhibitor (Roche, Indianapolis, IN, USA), and 2 mM Na3 VO4 (Fisher Scientific). .. Cell homogenates were mixed with an equal volume of 2× standard SDS sample loading buffer (Invitrogen) and loaded onto gels.

Article Title: All-trans retinoic acid increases the expression of oxidative myosin heavy chain through the PPARδ pathway in bovine muscle cells derived from satellite cells
Article Snippet: .. At 96 h of treatment, cultured bovine satellite cells were isolated using 200 μL of iced buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ), protease inhibitor (Roche, Switzerland), and 2 mM Na3 VO4 (Fisher Scientific). .. Samples were diluted with PBS to determine concentration via Pierce BCA protein assay (Thermo Fisher Science).

Protein Extraction:

Article Title: Blockade of Protein Geranylgeranylation Inhibits Cdk2-Dependent p27Kip1 Phosphorylation on Thr187 and Accumulates p27Kip1 in the Nucleus: Implications for Breast Cancer Therapy ▿ in the Nucleus: Implications for Breast Cancer Therapy ▿ §
Article Snippet: .. Weighted tumor samples were mixed with the appropriate amount of the T-PER tissue protein extraction reagent (Pierce, Rockford, IL) containing protease inhibitor cocktail, 2 mM PMSF, 2 mM Na3 VO4 , and 6.4 mg/ml p -nitrophenylphosphate and homogenized with the PCR tissue homogenizing kit (Fisher Scientific). ..

Article Title: Zinc Methionine Supplementation Impacts Gene and Protein Expression in Calf-Fed Holstein Steers with Minimal Impact on Feedlot Performance
Article Snippet: .. Adipose tissue protein was isolated with an ice-cold buffer containing tissue protein extraction reagent (T-PER; Fisher Scientific, Fair Lawn, NJ), protein inhibitor (Roche, Branchburg, NJ), and 2 mM Na3 VO4 (Fisher Scientific) at a 1:5 ratio. ..

Isolation:

Article Title: Chromium Propionate Enhances Adipogenic Differentiation of Bovine Intramuscular Adipocytes
Article Snippet: .. Western blotting Protein from cultured BSC and preadipocytes (IM and SC) was isolated with 200 μL of ice-cold buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ, USA), protein inhibitor (Roche, Indianapolis, IN, USA), and 2 mM Na3 VO4 (Fisher Scientific). .. Cell homogenates were mixed with an equal volume of 2× standard SDS sample loading buffer (Invitrogen) and loaded onto gels.

Article Title: Zinc Methionine Supplementation Impacts Gene and Protein Expression in Calf-Fed Holstein Steers with Minimal Impact on Feedlot Performance
Article Snippet: .. Adipose tissue protein was isolated with an ice-cold buffer containing tissue protein extraction reagent (T-PER; Fisher Scientific, Fair Lawn, NJ), protein inhibitor (Roche, Branchburg, NJ), and 2 mM Na3 VO4 (Fisher Scientific) at a 1:5 ratio. ..

Article Title: All-trans retinoic acid increases the expression of oxidative myosin heavy chain through the PPARδ pathway in bovine muscle cells derived from satellite cells
Article Snippet: .. At 96 h of treatment, cultured bovine satellite cells were isolated using 200 μL of iced buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ), protease inhibitor (Roche, Switzerland), and 2 mM Na3 VO4 (Fisher Scientific). .. Samples were diluted with PBS to determine concentration via Pierce BCA protein assay (Thermo Fisher Science).

Polymerase Chain Reaction:

Article Title: Blockade of Protein Geranylgeranylation Inhibits Cdk2-Dependent p27Kip1 Phosphorylation on Thr187 and Accumulates p27Kip1 in the Nucleus: Implications for Breast Cancer Therapy ▿ in the Nucleus: Implications for Breast Cancer Therapy ▿ §
Article Snippet: .. Weighted tumor samples were mixed with the appropriate amount of the T-PER tissue protein extraction reagent (Pierce, Rockford, IL) containing protease inhibitor cocktail, 2 mM PMSF, 2 mM Na3 VO4 , and 6.4 mg/ml p -nitrophenylphosphate and homogenized with the PCR tissue homogenizing kit (Fisher Scientific). ..

Western Blot:

Article Title: Chromium Propionate Enhances Adipogenic Differentiation of Bovine Intramuscular Adipocytes
Article Snippet: .. Western blotting Protein from cultured BSC and preadipocytes (IM and SC) was isolated with 200 μL of ice-cold buffer containing M-PER (Fisher Scientific, Fair Lawn, NJ, USA), protein inhibitor (Roche, Indianapolis, IN, USA), and 2 mM Na3 VO4 (Fisher Scientific). .. Cell homogenates were mixed with an equal volume of 2× standard SDS sample loading buffer (Invitrogen) and loaded onto gels.

Article Title: Detecting new microRNAs in human osteoarthritic chondrocytes identifies miR-3085 as a human, chondrocyte-selective, microRNA
Article Snippet: .. Western blot Whole cell lysates were harvested into ice cold RIPA buffer (50 mM Tris-HCL pH7.6, 150 mM NaCl, 1% (v/v) Triton x-100, 1% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 10 mM NaF, 2 mM Na3 VO4 , 1x protease cocktail III tablet (Fisher Scientific)). .. Samples were separated on reducing SDS-PAGE, transferred to PVDF membrane and probed overnight at 4°C.

Lysis:

Article Title: Comprehensive proteome quantification reveals NgBR as a new regulator for Epithelial-Mesenchymal Transition of breast tumor cells
Article Snippet: .. Total cell lysates were prepared by adding 200 μL of cell lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM Na3 VO4 , 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, and 1 μg/mL leupeptin) and briefly homogenized with Fisher Scientific Sonic Dismembrator Model 500. ..

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  • 92
    Fisher Scientific na3 vo4
    Na3 Vo4, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Fisher Scientific na3 vo4 orthovanadate
    Na3 Vo4 Orthovanadate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4 orthovanadate/product/Fisher Scientific
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 orthovanadate - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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