Structured Review

FUJIFILM na3 vo4
Na3 Vo4, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/FUJIFILM
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Protease Inhibitor:

Article Title: A method of producing genetically manipulated mouse mammary gland
Article Snippet: .. Cells were collected in RIPA buffer (10 mM Tris-HCl (pH 8.0), 1% (w/v) NP40, 0.1% (w/v) sodium deoxycholate (Wako), 0.1% ( w/v ) SDS (Wako), 0.15 M NaCl (Wako), 1 mM EDTA, 10 mM NaF (Wako), 1.5 mM Na3 VO4 (Wako), and cOmplete™ Protease Inhibitor Cocktail (Roche)). .. Protein concentrations were titered using the BCA protein assay kit (Thermo Fisher Scientific).

Article Title: Brexpiprazole, a Serotonin-Dopamine Activity Modulator, Can Sensitize Glioma Stem Cells to Osimertinib, a Third-Generation EGFR-TKI, via Survivin Reduction
Article Snippet: .. Immunoblot Analysis Cells were washed with ice-cold PBS and lysed in RIPA buffer (10 mM Tris-HCl (pH 7.4), 0.1% SDS, 0.1% sodium deoxycholate, 1% NP-40, 150 mM NaCl, 1 mM EDTA, 1.5 mM Na3 VO4 , 10 mM NaF, 10 mM sodium pyrophosphate, 10 mM sodium β-glycerophosphate. and 1% protease inhibitor cocktail set III (Wako Pure Chemical Industries, Ltd, Osaka, Japan)). .. After centrifugation for 10 minutes at 14,000× g at 4 °C, the supernatants were harvested as the cell lysates and the protein concentration of the cell lysates was measured using the BCA protein assay kit (Thermo Fisher Scientific).

Article Title: RB1CC1 Activates RB1 Pathway and Inhibits Proliferation and Cologenic Survival in Human Cancer
Article Snippet: .. Immunoprecipitation of protein complex followed by liquid chromatography- tandem mass spectrometry (LC-MS/MS) The MCF-7 breast cancer cells were lysed in EBC lysis buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40 (NP-40), 0.25% sodium deoxycholate, 0.2 mM Na3 VO4 , 100mM NaF and 1% protease inhibitor cocktail (Wako Chemicals, Japan), and then centrifuged for 20 min at 20,000×g at 4°C. .. The 5 ml supernatant (∼10mg/ml) was incubated with 100 µg of anti-RB1CC1 purified IgG primary antibody at 4°C overnight.

Article Title: A method of producing genetically manipulated mouse mammary gland
Article Snippet: .. Immunoblotting Cells were collected in RIPA buffer (10 mM Tris-HCl (pH 8.0), 1% (w/v) NP40, 0.1% (w/v) sodium deoxycholate (Wako), 0.1% (w/v ) SDS (Wako), 0.15 M NaCl (Wako), 1 mM EDTA, 10 mM NaF (Wako), 1.5 mM Na3 VO4 (Wako), and cOmplete™ Protease Inhibitor Cocktail (Roche)). .. Protein concentrations were titered using the BCA protein assay kit (Thermo Fisher Scientific).

Immunoprecipitation:

Article Title: RB1CC1 Activates RB1 Pathway and Inhibits Proliferation and Cologenic Survival in Human Cancer
Article Snippet: .. Immunoprecipitation of protein complex followed by liquid chromatography- tandem mass spectrometry (LC-MS/MS) The MCF-7 breast cancer cells were lysed in EBC lysis buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40 (NP-40), 0.25% sodium deoxycholate, 0.2 mM Na3 VO4 , 100mM NaF and 1% protease inhibitor cocktail (Wako Chemicals, Japan), and then centrifuged for 20 min at 20,000×g at 4°C. .. The 5 ml supernatant (∼10mg/ml) was incubated with 100 µg of anti-RB1CC1 purified IgG primary antibody at 4°C overnight.

Liquid Chromatography:

Article Title: RB1CC1 Activates RB1 Pathway and Inhibits Proliferation and Cologenic Survival in Human Cancer
Article Snippet: .. Immunoprecipitation of protein complex followed by liquid chromatography- tandem mass spectrometry (LC-MS/MS) The MCF-7 breast cancer cells were lysed in EBC lysis buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40 (NP-40), 0.25% sodium deoxycholate, 0.2 mM Na3 VO4 , 100mM NaF and 1% protease inhibitor cocktail (Wako Chemicals, Japan), and then centrifuged for 20 min at 20,000×g at 4°C. .. The 5 ml supernatant (∼10mg/ml) was incubated with 100 µg of anti-RB1CC1 purified IgG primary antibody at 4°C overnight.

Mass Spectrometry:

Article Title: RB1CC1 Activates RB1 Pathway and Inhibits Proliferation and Cologenic Survival in Human Cancer
Article Snippet: .. Immunoprecipitation of protein complex followed by liquid chromatography- tandem mass spectrometry (LC-MS/MS) The MCF-7 breast cancer cells were lysed in EBC lysis buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40 (NP-40), 0.25% sodium deoxycholate, 0.2 mM Na3 VO4 , 100mM NaF and 1% protease inhibitor cocktail (Wako Chemicals, Japan), and then centrifuged for 20 min at 20,000×g at 4°C. .. The 5 ml supernatant (∼10mg/ml) was incubated with 100 µg of anti-RB1CC1 purified IgG primary antibody at 4°C overnight.

Lysis:

Article Title: RB1CC1 Activates RB1 Pathway and Inhibits Proliferation and Cologenic Survival in Human Cancer
Article Snippet: .. Immunoprecipitation of protein complex followed by liquid chromatography- tandem mass spectrometry (LC-MS/MS) The MCF-7 breast cancer cells were lysed in EBC lysis buffer containing 50 mM Tris-HCl (pH 8.0), 120 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40 (NP-40), 0.25% sodium deoxycholate, 0.2 mM Na3 VO4 , 100mM NaF and 1% protease inhibitor cocktail (Wako Chemicals, Japan), and then centrifuged for 20 min at 20,000×g at 4°C. .. The 5 ml supernatant (∼10mg/ml) was incubated with 100 µg of anti-RB1CC1 purified IgG primary antibody at 4°C overnight.

Article Title: Lectin-dependent localization of cell surface sialic acid-binding lectin Siglec-9
Article Snippet: .. RAW264 cells (2 × 108 ) were lysed in 1.5 ml of lysis buffer (TNE buffer (25 mM Tris–HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA) containing 1 % Triton X100 (Calbiochem, La Jolla, CA, USA), 20 μg/ml aprotinin (Wako Pure Chemical Industries), 100 μg/ml phenylmethylsulfonylfluoride (Wako Pure Chemical Industries), 1 mM Na3 VO4 (Wako Pure Chemical Industries), 10 μM Na2 MoO4 (Wako Pure Chemical Industries), and 10 mM NaF (Wako Pure Chemical Industries)) for 1 h on ice. .. The membrane fraction was collected after centrifugation at 9,200× g for 10 min.