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Cell Signaling Technology Inc na3 vo4
Na3 Vo4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Cell Signaling Technology Inc
Average 94 stars, based on 126 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
94/100 stars

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Clone Assay:

Article Title: Differential Expression of Tie2 Receptor and VEGFR2 by Endothelial Clones Derived from Isolated Bovine Mononuclear Cells
Article Snippet: .. Western Blot Analysis In order to characterize the expression patterns of the endothelial clones obtained, cells were grown to confluence and lysed using cell lysis buffer, containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 µg/mL leupeptin, 1 mM PMF and 2 µg/mL aprotinine (Cell Signaling Technology, Danvers, MA, USA). .. Proteins were separated using SDS-PAGE on 7.5% polyacrylamide gel, transferred to a PVDF membrane (Roche, Laval, Quebec, Canada) using wet transfer, blocked using 5% milk for 1 hour and probed overnight using either goat anti-CD31 (1∶1000, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-VEGFR2 (1∶1000, Cell Signaling Technology), mouse anti-N-cadherin (1∶2500, BD Biosciences), mouse anti-α-smooth muscle actin (1∶1000, Sigma-Aldrich), rabbit anti-PDGFRβ (1∶1000, Cell Signaling Techlonogy), mouse anti-Tie2 (1∶1000, Cell Signaling Technology) or mouse anti-VEGFR1 (1∶1000, AbCam, Cambridge, MA, USA).

Co-Immunoprecipitation Assay:

Article Title: ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer
Article Snippet: .. Western blotting and co-IP Cells for Western blot analysis were lysed in 1× Cell Signaling lysis buffer [20 mM tris-HCl, (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4, and leupeptin (1 μg/ml) (Cell Signaling Technologies)] supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF). .. For co-IP experiments, cells were lysed in CHAPS lysis buffer [40 mM Hepes (pH 7.4), 1 mM EDTA, 120 mM NaCl, 10 mM sodium pyrophosphate, 10 mM β-glycerophosphate, 0.3% CHAPS, 50 mM NaF, 1.5 mM NaVO, 1 mM PMSF, and one tablet of EDTA-free protease inhibitors (Roche) per 10 ml of solution].

Peptide Mass Fingerprinting:

Article Title: Differential Expression of Tie2 Receptor and VEGFR2 by Endothelial Clones Derived from Isolated Bovine Mononuclear Cells
Article Snippet: .. Western Blot Analysis In order to characterize the expression patterns of the endothelial clones obtained, cells were grown to confluence and lysed using cell lysis buffer, containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 µg/mL leupeptin, 1 mM PMF and 2 µg/mL aprotinine (Cell Signaling Technology, Danvers, MA, USA). .. Proteins were separated using SDS-PAGE on 7.5% polyacrylamide gel, transferred to a PVDF membrane (Roche, Laval, Quebec, Canada) using wet transfer, blocked using 5% milk for 1 hour and probed overnight using either goat anti-CD31 (1∶1000, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-VEGFR2 (1∶1000, Cell Signaling Technology), mouse anti-N-cadherin (1∶2500, BD Biosciences), mouse anti-α-smooth muscle actin (1∶1000, Sigma-Aldrich), rabbit anti-PDGFRβ (1∶1000, Cell Signaling Techlonogy), mouse anti-Tie2 (1∶1000, Cell Signaling Technology) or mouse anti-VEGFR1 (1∶1000, AbCam, Cambridge, MA, USA).

Sonication:

Article Title: Mechanisms Underlying Chronic Binge Alcohol Exposure-Induced Uterine Artery Dysfunction in Pregnant Rat
Article Snippet: .. The arteries were homogenized and sonicated in 80–100μl of ice cold RIPA buffer, pH 7.5 (220 mM Tris-HCl, 150 mM NaCl, 1 mM Na2 , EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3 VO4 , 1 μg/ml leupeptin; Cell Signaling Technologies #9806), and immediately placed on ice. .. Protein concentration measurement was performed using Pierce™ BCA protein assay kit instructions (ThermoFisher Scientific).

Radio Immunoprecipitation:

Article Title: Macrophage migration inhibitory factor downregulation: a novel mechanism of resistance to anti-angiogenic therapy
Article Snippet: .. Western blot Human tissue and cellular preparations were harvested in radio immunoprecipitation buffer (RIPA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycorophosphate, 1 mM Na3 VO4 , 1 μg/ml leupeptin (RIPA Buffer, 10x, CST#9806, Cell Signaling, Danvers, MA, USA) and one tablet each of PhosStop and Complete Mini (Roche, Indianapolis, IN, USA). .. Insoluble materials were removed by centrifugation at 14 000 r.p.m. for 20 min at 4 °C.

Protease Inhibitor:

Article Title: Absence of integrin α3β1 promotes the progression of HER2-driven breast cancer in vivo
Article Snippet: .. Protein lysates of carcinoma cells were obtained from subconfluent cell cultures, washed in cold PBS, and lysed in RIPA buffer (20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 4 mM EDTA (pH 7.5), 1% Nonidet P-40, 0.1% SDS, 0.5% sodium deoxycholate) supplemented with 1.5 mM Na3 VO4 , 15 mM NaF (Cell Signaling) and protease inhibitor cocktail (Sigma). .. Lysates were cleared by centrifugation at 14.000×g for 20 min at 4 °C and eluted in sample buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 12.5 mM EDTA, 0.02% bromophenol blue) containing a final concentration of 2% β-mercaptoethanol and denatured at 95 °C for 10 min. Proteins were separated by electrophoresis using Bolt Novex 4–12% gradient Bis-Tris gels (Invitrogen), transferred to Immobilon-P transfer membranes (Millipore Corp), and blocked for 1 h in 2% BSA in TBST buffer (10 mM Tris (pH 7.5), 150 mM NaCl, and 0.3% Tween-20).

Cell Culture:

Article Title: Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells
Article Snippet: .. 2.3 Western blotting analysis LLC-PK1 cells cultured in six-well plates were treated with ginsenoside Rb1 (10μΜ or 50μΜ) for 24 h; then, the cells were lysed with RIPA buffer (20mM Tris–HCl (pH 7.5), 150mM NaCl, 1mM Na2 EDTA, 1mM EGTA [ethylene glycol-bis(β-aminoethyl ether)-N ,N ,N ′,N ′-tetraacetic acid], 1% NP-40, 1% sodium deoxycholate, 2.5mM sodium pyrophosphate, 1mM β-glycerophosphate, 1mM Na3 VO4 , and 1 μg/mL leupeptin (Cell Signaling, Danvers, MA, USA), and supplemented with 1mM phenylmethylsulfonyl fluoride immediately prior to use. .. Each protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA).

Incubation:

Article Title: B7-H4 is highly expressed in aggressive Epstein-Barr virus positive diffuse large B-cell lymphoma and inhibits apoptosis through upregulating Erk1/2 and Akt signalling pathways
Article Snippet: .. Western blots The cells (1 × 107 cells/sample) were washed in cold PBS and lysed in cell lysis buffer (20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , and 1 μg/ml leupeptin) (Cell Signaling Technology), and incubated on ice for 30 min. .. The insoluble material was removed by centrifugation at 8,000 g for 10 min.

Expressing:

Article Title: Differential Expression of Tie2 Receptor and VEGFR2 by Endothelial Clones Derived from Isolated Bovine Mononuclear Cells
Article Snippet: .. Western Blot Analysis In order to characterize the expression patterns of the endothelial clones obtained, cells were grown to confluence and lysed using cell lysis buffer, containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 µg/mL leupeptin, 1 mM PMF and 2 µg/mL aprotinine (Cell Signaling Technology, Danvers, MA, USA). .. Proteins were separated using SDS-PAGE on 7.5% polyacrylamide gel, transferred to a PVDF membrane (Roche, Laval, Quebec, Canada) using wet transfer, blocked using 5% milk for 1 hour and probed overnight using either goat anti-CD31 (1∶1000, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-VEGFR2 (1∶1000, Cell Signaling Technology), mouse anti-N-cadherin (1∶2500, BD Biosciences), mouse anti-α-smooth muscle actin (1∶1000, Sigma-Aldrich), rabbit anti-PDGFRβ (1∶1000, Cell Signaling Techlonogy), mouse anti-Tie2 (1∶1000, Cell Signaling Technology) or mouse anti-VEGFR1 (1∶1000, AbCam, Cambridge, MA, USA).

Lysis:

Article Title: Differential Expression of Tie2 Receptor and VEGFR2 by Endothelial Clones Derived from Isolated Bovine Mononuclear Cells
Article Snippet: .. Western Blot Analysis In order to characterize the expression patterns of the endothelial clones obtained, cells were grown to confluence and lysed using cell lysis buffer, containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 µg/mL leupeptin, 1 mM PMF and 2 µg/mL aprotinine (Cell Signaling Technology, Danvers, MA, USA). .. Proteins were separated using SDS-PAGE on 7.5% polyacrylamide gel, transferred to a PVDF membrane (Roche, Laval, Quebec, Canada) using wet transfer, blocked using 5% milk for 1 hour and probed overnight using either goat anti-CD31 (1∶1000, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-VEGFR2 (1∶1000, Cell Signaling Technology), mouse anti-N-cadherin (1∶2500, BD Biosciences), mouse anti-α-smooth muscle actin (1∶1000, Sigma-Aldrich), rabbit anti-PDGFRβ (1∶1000, Cell Signaling Techlonogy), mouse anti-Tie2 (1∶1000, Cell Signaling Technology) or mouse anti-VEGFR1 (1∶1000, AbCam, Cambridge, MA, USA).

Article Title: B7-H4 is highly expressed in aggressive Epstein-Barr virus positive diffuse large B-cell lymphoma and inhibits apoptosis through upregulating Erk1/2 and Akt signalling pathways
Article Snippet: .. Western blots The cells (1 × 107 cells/sample) were washed in cold PBS and lysed in cell lysis buffer (20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , and 1 μg/ml leupeptin) (Cell Signaling Technology), and incubated on ice for 30 min. .. The insoluble material was removed by centrifugation at 8,000 g for 10 min.

Article Title: ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer
Article Snippet: .. Western blotting and co-IP Cells for Western blot analysis were lysed in 1× Cell Signaling lysis buffer [20 mM tris-HCl, (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4, and leupeptin (1 μg/ml) (Cell Signaling Technologies)] supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF). .. For co-IP experiments, cells were lysed in CHAPS lysis buffer [40 mM Hepes (pH 7.4), 1 mM EDTA, 120 mM NaCl, 10 mM sodium pyrophosphate, 10 mM β-glycerophosphate, 0.3% CHAPS, 50 mM NaF, 1.5 mM NaVO, 1 mM PMSF, and one tablet of EDTA-free protease inhibitors (Roche) per 10 ml of solution].

Western Blot:

Article Title: Differential Expression of Tie2 Receptor and VEGFR2 by Endothelial Clones Derived from Isolated Bovine Mononuclear Cells
Article Snippet: .. Western Blot Analysis In order to characterize the expression patterns of the endothelial clones obtained, cells were grown to confluence and lysed using cell lysis buffer, containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton-X, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 µg/mL leupeptin, 1 mM PMF and 2 µg/mL aprotinine (Cell Signaling Technology, Danvers, MA, USA). .. Proteins were separated using SDS-PAGE on 7.5% polyacrylamide gel, transferred to a PVDF membrane (Roche, Laval, Quebec, Canada) using wet transfer, blocked using 5% milk for 1 hour and probed overnight using either goat anti-CD31 (1∶1000, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-VEGFR2 (1∶1000, Cell Signaling Technology), mouse anti-N-cadherin (1∶2500, BD Biosciences), mouse anti-α-smooth muscle actin (1∶1000, Sigma-Aldrich), rabbit anti-PDGFRβ (1∶1000, Cell Signaling Techlonogy), mouse anti-Tie2 (1∶1000, Cell Signaling Technology) or mouse anti-VEGFR1 (1∶1000, AbCam, Cambridge, MA, USA).

Article Title: Macrophage migration inhibitory factor downregulation: a novel mechanism of resistance to anti-angiogenic therapy
Article Snippet: .. Western blot Human tissue and cellular preparations were harvested in radio immunoprecipitation buffer (RIPA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycorophosphate, 1 mM Na3 VO4 , 1 μg/ml leupeptin (RIPA Buffer, 10x, CST#9806, Cell Signaling, Danvers, MA, USA) and one tablet each of PhosStop and Complete Mini (Roche, Indianapolis, IN, USA). .. Insoluble materials were removed by centrifugation at 14 000 r.p.m. for 20 min at 4 °C.

Article Title: Agrimonolide and Desmethylagrimonolide Induced HO-1 Expression in HepG2 Cells through Nrf2-Transduction and p38 Inactivation
Article Snippet: .. Western blotting The cells were harvested, washed twice with PBS buffer, and lysised in RIPA buffer consisting of 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , and 1 μg/mL leupeptin (Cell Signaling Technology; Beverly, MA, USA). ..

Article Title: B7-H4 is highly expressed in aggressive Epstein-Barr virus positive diffuse large B-cell lymphoma and inhibits apoptosis through upregulating Erk1/2 and Akt signalling pathways
Article Snippet: .. Western blots The cells (1 × 107 cells/sample) were washed in cold PBS and lysed in cell lysis buffer (20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , and 1 μg/ml leupeptin) (Cell Signaling Technology), and incubated on ice for 30 min. .. The insoluble material was removed by centrifugation at 8,000 g for 10 min.

Article Title: ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer
Article Snippet: .. Western blotting and co-IP Cells for Western blot analysis were lysed in 1× Cell Signaling lysis buffer [20 mM tris-HCl, (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4, and leupeptin (1 μg/ml) (Cell Signaling Technologies)] supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF). .. For co-IP experiments, cells were lysed in CHAPS lysis buffer [40 mM Hepes (pH 7.4), 1 mM EDTA, 120 mM NaCl, 10 mM sodium pyrophosphate, 10 mM β-glycerophosphate, 0.3% CHAPS, 50 mM NaF, 1.5 mM NaVO, 1 mM PMSF, and one tablet of EDTA-free protease inhibitors (Roche) per 10 ml of solution].

Article Title: Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells
Article Snippet: .. 2.3 Western blotting analysis LLC-PK1 cells cultured in six-well plates were treated with ginsenoside Rb1 (10μΜ or 50μΜ) for 24 h; then, the cells were lysed with RIPA buffer (20mM Tris–HCl (pH 7.5), 150mM NaCl, 1mM Na2 EDTA, 1mM EGTA [ethylene glycol-bis(β-aminoethyl ether)-N ,N ,N ′,N ′-tetraacetic acid], 1% NP-40, 1% sodium deoxycholate, 2.5mM sodium pyrophosphate, 1mM β-glycerophosphate, 1mM Na3 VO4 , and 1 μg/mL leupeptin (Cell Signaling, Danvers, MA, USA), and supplemented with 1mM phenylmethylsulfonyl fluoride immediately prior to use. .. Each protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA).

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  • 94
    Cell Signaling Technology Inc na3 vo4
    Na3 Vo4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Cell Signaling Technology Inc
    Average 94 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc m na3 vo4
    M Na3 Vo4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Cell Signaling Technology Inc
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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