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BioShop na3 vo4
Na3 Vo4, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/BioShop
Average 92 stars, based on 12 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Centrifugation:

Article Title: PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication
Article Snippet: .. Briefly, C6/36 cells were harvested at various intervals of time post-infection by centrifugation of 3000 rpm at 4 °C for 10 min. Pellets were re-suspended in 100 μL of RIPA lysis buffer consisting of 50 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA, 0.25% deoxycholic acid, 1% NP-40, 0.01% Triton-X 100, 1 M NaF, 200 mM Na3 VO4 , 1× protease inhibitor cocktail (BioShop) and 1× serine/threonine phosphatase inhibitor cocktail (Bionovas). .. Subsequently, whole-cell extracts were run on 12% or 15% SDS-polyacrylamide gels, and separated proteins were electrically transferred onto polyvinylidene difluoride (PVDF) membranes with a 0.45-μm pore size (EMD Millipore).

Isolation:

Article Title: Ste20-like kinase, SLK, a novel mediator of podocyte integrity
Article Snippet: .. Lysates of glomeruli isolated by differential sieving ( ) were prepared in buffer containing 1% Triton X-100, 125 mM NaCl, 10 mM Tris (pH 7.4), 1 mM EGTA, 2 mM Na3 VO4 , 10 mM sodium pyrophosphate, 25 mM NaF, and protease inhibitor cocktail (Bioshop, Burlington, ON). .. Equal amounts of lysate proteins were dissolved in Laemmli buffer and subjected to SDS-PAGE under reducing conditions.

Protein Extraction:

Article Title: Regulation of Torpor in the Gray Mouse Lemur: Transcriptional and Translational Controls and Role of AMPK Signaling
Article Snippet: .. Protein extraction Frozen tissue samples (up to 50 mg) were homogenized 1:4 (w/v) in a pre-chilled lysis buffer (Millipore, catalog No. 43-040) with 1 mM Na3 VO4 , 10 mM ß-glycerophosphate and 1% protease inhibitor cocktail (catalog No. PIC001, BioShop) added using a Dounce homogenizer. .. Supernatants were collected as total soluble protein lysates and protein concentrations were determined using the Bradford assay.

Article Title: Expression Profiling and Structural Characterization of MicroRNAs in Adipose Tissues of Hibernating Ground Squirrels
Article Snippet: .. Protein extraction and immunoblotting Samples of frozen BAT and WAT (0.5 g) were homogenized using a Polytron PT 1000 homogenizer at a 1:2 (weight/volume) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, 0.1 mM EDTA, 10 mM NaF, 1 mM Na3 VO4 , and 10 mM β-glycerol-phosphate, supplemented with 1 μL of protease inhibitor cocktail (BioShop, Burlington, ON, Canada). ..

Protease Inhibitor:

Article Title: PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication
Article Snippet: .. In brief, C6/36 cells were lysed in NP40 lysis buffer (50 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA and 1% NP-40) with 1 M NaF, 200 mM Na3 VO4 , 1× protease inhibitor cocktail (BioShop, Burlingtom, ON, Canada) and 1× serine/threonine phosphatase inhibitor cocktail (Bionovas, Toronto, ON, Canada). .. About 500–1000 μg of cell lysates were incubated with 20–30 μL of beads for 6 h at 4 °C with gentle rotation.

Article Title: Regulation of Torpor in the Gray Mouse Lemur: Transcriptional and Translational Controls and Role of AMPK Signaling
Article Snippet: .. Protein extraction Frozen tissue samples (up to 50 mg) were homogenized 1:4 (w/v) in a pre-chilled lysis buffer (Millipore, catalog No. 43-040) with 1 mM Na3 VO4 , 10 mM ß-glycerophosphate and 1% protease inhibitor cocktail (catalog No. PIC001, BioShop) added using a Dounce homogenizer. .. Supernatants were collected as total soluble protein lysates and protein concentrations were determined using the Bradford assay.

Article Title: Expression Profiling and Structural Characterization of MicroRNAs in Adipose Tissues of Hibernating Ground Squirrels
Article Snippet: .. Protein extraction and immunoblotting Samples of frozen BAT and WAT (0.5 g) were homogenized using a Polytron PT 1000 homogenizer at a 1:2 (weight/volume) in buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, 0.1 mM EDTA, 10 mM NaF, 1 mM Na3 VO4 , and 10 mM β-glycerol-phosphate, supplemented with 1 μL of protease inhibitor cocktail (BioShop, Burlington, ON, Canada). ..

Article Title: PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication
Article Snippet: .. Briefly, C6/36 cells were harvested at various intervals of time post-infection by centrifugation of 3000 rpm at 4 °C for 10 min. Pellets were re-suspended in 100 μL of RIPA lysis buffer consisting of 50 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA, 0.25% deoxycholic acid, 1% NP-40, 0.01% Triton-X 100, 1 M NaF, 200 mM Na3 VO4 , 1× protease inhibitor cocktail (BioShop) and 1× serine/threonine phosphatase inhibitor cocktail (Bionovas). .. Subsequently, whole-cell extracts were run on 12% or 15% SDS-polyacrylamide gels, and separated proteins were electrically transferred onto polyvinylidene difluoride (PVDF) membranes with a 0.45-μm pore size (EMD Millipore).

Article Title: Ste20-like kinase, SLK, a novel mediator of podocyte integrity
Article Snippet: .. Lysates of glomeruli isolated by differential sieving ( ) were prepared in buffer containing 1% Triton X-100, 125 mM NaCl, 10 mM Tris (pH 7.4), 1 mM EGTA, 2 mM Na3 VO4 , 10 mM sodium pyrophosphate, 25 mM NaF, and protease inhibitor cocktail (Bioshop, Burlington, ON). .. Equal amounts of lysate proteins were dissolved in Laemmli buffer and subjected to SDS-PAGE under reducing conditions.

Modification:

Article Title: The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment
Article Snippet: .. Immunoblot analysis Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3 VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). .. Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250).

Lysis:

Article Title: The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment
Article Snippet: .. Immunoblot analysis Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3 VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). .. Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250).

Article Title: PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication
Article Snippet: .. In brief, C6/36 cells were lysed in NP40 lysis buffer (50 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA and 1% NP-40) with 1 M NaF, 200 mM Na3 VO4 , 1× protease inhibitor cocktail (BioShop, Burlingtom, ON, Canada) and 1× serine/threonine phosphatase inhibitor cocktail (Bionovas, Toronto, ON, Canada). .. About 500–1000 μg of cell lysates were incubated with 20–30 μL of beads for 6 h at 4 °C with gentle rotation.

Article Title: Regulation of Torpor in the Gray Mouse Lemur: Transcriptional and Translational Controls and Role of AMPK Signaling
Article Snippet: .. Protein extraction Frozen tissue samples (up to 50 mg) were homogenized 1:4 (w/v) in a pre-chilled lysis buffer (Millipore, catalog No. 43-040) with 1 mM Na3 VO4 , 10 mM ß-glycerophosphate and 1% protease inhibitor cocktail (catalog No. PIC001, BioShop) added using a Dounce homogenizer. .. Supernatants were collected as total soluble protein lysates and protein concentrations were determined using the Bradford assay.

Article Title: PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication
Article Snippet: .. Briefly, C6/36 cells were harvested at various intervals of time post-infection by centrifugation of 3000 rpm at 4 °C for 10 min. Pellets were re-suspended in 100 μL of RIPA lysis buffer consisting of 50 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA, 0.25% deoxycholic acid, 1% NP-40, 0.01% Triton-X 100, 1 M NaF, 200 mM Na3 VO4 , 1× protease inhibitor cocktail (BioShop) and 1× serine/threonine phosphatase inhibitor cocktail (Bionovas). .. Subsequently, whole-cell extracts were run on 12% or 15% SDS-polyacrylamide gels, and separated proteins were electrically transferred onto polyvinylidene difluoride (PVDF) membranes with a 0.45-μm pore size (EMD Millipore).

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    BioShop na3 vo4
    Na3 Vo4, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/BioShop
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    BioShop homogenizing buffer
    Homogenizing Buffer, supplied by BioShop, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homogenizing buffer/product/BioShop
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    homogenizing buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    90
    BioShop m na3 vo4
    M Na3 Vo4, supplied by BioShop, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/BioShop
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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