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Beyotime na3 vo4
Na3 Vo4, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Beyotime
Average 93 stars, based on 38 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
93/100 stars

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Co-Immunoprecipitation Assay:

Article Title: MicroRNA-32 silences WWP2 expression to maintain the pluripotency of human amniotic epithelial stem cells and β islet-like cell differentiation
Article Snippet: .. The cells were then lysed in 500 µ l modified cell lysis buffer [20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, sodium pyrophosphate, β-glycerophosphate, Na3 VO4 and leupeptin) (Beyotime Institute of Biotechnology) for western blotting and co-IP. .. After lysis, each sample was clarified by centrifugation to remove insoluble debris and the supernatant was preincubated with 20 µ g protein A agarose beads (Beyotime Institute of Biotechnology) for 30 min at 4°C with agitation.

Western Blot:

Article Title: Lentivirus-based RNA Silencing of Nemo-like Kinase (NLK) Inhibits the CAL 27 Human Adenosquamos Carcinoma Cells Proliferation and Blocks G0/G1 Phase to S Phase
Article Snippet: .. Western blot After infecting with lentivirus, CAL-27 cells were collected and lysed with RIPA buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3 VO4 , 1 µg/ml leupeptin) with PMSF (Beyotime Biotechnology, Jiangsu, China). .. Protein samples were then separated by SDS-PAGE and transferred to the PVDF membranes.

Article Title: Linarin prevents LPS-induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF-κB pathways
Article Snippet: .. Western blot analysis The total protein of lung tissue samples and cells was extracted by addition of cold lysis buffer [10 mM Tris-HCl, 1 mM EDTA, and 250 mM sucrose, pH 7.4, containing 15 µ g/ml aprotinin, 5 µ g/ml leupeptin, 0.1 mM phenylmeth-anesulfonyl fluoride (PMSF), 1 mM NaF, and 1 mM Na3 VO4 (Beyotime Institute of Biotechnology)] for 50 min at 4°C. .. The soluble protein concentrations in the lysates were assessed using a BCA Protein Assay kit.

Article Title: Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2
Article Snippet: .. Western Blot Cells at density of 1∗ 106 -107 cells/well were rinsed twice with phosphate-buffered saline (PBS) and then lysed on ice with 200 μ l cold cell lysis buffer for Western and IP containing 20 mM Tris-HCl (pH = 7.5), 150 mM NaCl, 1% TritonX-100, 2.5 mM sodium pyrophosphate, 1% Na3 VO4 , 1 mM EDTA, 0.5 μ g/ml leupeptin, and 1 mM phenylmethylsulfonyl fluoride (PMSF) (Beyotime Biotechnology). ..

Article Title: MicroRNA-32 silences WWP2 expression to maintain the pluripotency of human amniotic epithelial stem cells and β islet-like cell differentiation
Article Snippet: .. The cells were then lysed in 500 µ l modified cell lysis buffer [20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, sodium pyrophosphate, β-glycerophosphate, Na3 VO4 and leupeptin) (Beyotime Institute of Biotechnology) for western blotting and co-IP. .. After lysis, each sample was clarified by centrifugation to remove insoluble debris and the supernatant was preincubated with 20 µ g protein A agarose beads (Beyotime Institute of Biotechnology) for 30 min at 4°C with agitation.

Bradford Protein Assay:

Article Title: Oxidized Low-Density Lipoprotein-Dependent Platelet-Derived Microvesicles Trigger Procoagulant Effects and Amplify Oxidative Stress
Article Snippet: .. Platelets treated with PMVs or controls were lysed in 2 mmol/L Tris-HCl (pH 7.5), 150 mmol/L NaCl, 1 mmol/L EGTA, 1 mmol/L EDTA, 1% Triton X-100, 2.5 mmol/L sodium pyrophosphate, 1 mmol/L Na3 VO4 , 1 mmol/L phenylmethylsulfonyl fluoride and 1 μg/mL leupeptin, and protein concentrations were measured with use of a Bradford protein assay kit (Beyotime). ..

Lysis:

Article Title: Linarin prevents LPS-induced acute lung injury by suppressing oxidative stress and inflammation via inhibition of TXNIP/NLRP3 and NF-κB pathways
Article Snippet: .. Western blot analysis The total protein of lung tissue samples and cells was extracted by addition of cold lysis buffer [10 mM Tris-HCl, 1 mM EDTA, and 250 mM sucrose, pH 7.4, containing 15 µ g/ml aprotinin, 5 µ g/ml leupeptin, 0.1 mM phenylmeth-anesulfonyl fluoride (PMSF), 1 mM NaF, and 1 mM Na3 VO4 (Beyotime Institute of Biotechnology)] for 50 min at 4°C. .. The soluble protein concentrations in the lysates were assessed using a BCA Protein Assay kit.

Article Title: Acidic mammalian chitinase gene is highly expressed in the special oxyntic glands of Manis javanica
Article Snippet: .. Immunoblotting Tissue proteins from the three samples were prepared as follows: (a) The tissues were dissected on ice, and the excess blood was wash away; (b) the fresh tissue was placed in a clean mortar and ground in liquid nitrogen; (c) the tissue powder was ground into a precooling tube; (d) an appropriate cell lysis buffer was added, containing 20 mm Tris (pH 7.5), 150 mm NaCl, 1% Triton X‐100, and several protein inhibitors such as sodium pyrophosphate, β‐glycerophosphate, EDTA, Na3 VO4 , and leupeptin (Beyotime Biotechnology, Shanghai, China) according to the amount of tissue (1 mL for 0.1 mg tissue); (e) the supernatant was sonicated on ice for 15–20 min; (f) spin at 12 000 rpm for 15–20 min at 4 °C; and (g) the supernatant was transferred into a new tube that was kept on ice at all times. .. The protein content in the supernatant was quantified using a Bradford protein assay kit (Beyotime Biotechnology).

Article Title: Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2
Article Snippet: .. Western Blot Cells at density of 1∗ 106 -107 cells/well were rinsed twice with phosphate-buffered saline (PBS) and then lysed on ice with 200 μ l cold cell lysis buffer for Western and IP containing 20 mM Tris-HCl (pH = 7.5), 150 mM NaCl, 1% TritonX-100, 2.5 mM sodium pyrophosphate, 1% Na3 VO4 , 1 mM EDTA, 0.5 μ g/ml leupeptin, and 1 mM phenylmethylsulfonyl fluoride (PMSF) (Beyotime Biotechnology). ..

Article Title: Niclosamide enhances the cytotoxic effect of cisplatin in cisplatin-resistant human lung cancer cells via suppression of lung resistance-related protein and c-myc
Article Snippet: .. Lysis buffer [20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, sodium pyrophosphate, β-glycerophosphate, EDTA, Na3 VO4 and leupeptin was purchased from Beyotime Institute of Biotechnology (Shanghai, China). .. Total protein (~20 µg) was separated on a 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred to a polyvinylidene fluoride membrane.

Article Title: MicroRNA-32 silences WWP2 expression to maintain the pluripotency of human amniotic epithelial stem cells and β islet-like cell differentiation
Article Snippet: .. The cells were then lysed in 500 µ l modified cell lysis buffer [20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, sodium pyrophosphate, β-glycerophosphate, Na3 VO4 and leupeptin) (Beyotime Institute of Biotechnology) for western blotting and co-IP. .. After lysis, each sample was clarified by centrifugation to remove insoluble debris and the supernatant was preincubated with 20 µ g protein A agarose beads (Beyotime Institute of Biotechnology) for 30 min at 4°C with agitation.

Modification:

Article Title: MicroRNA-32 silences WWP2 expression to maintain the pluripotency of human amniotic epithelial stem cells and β islet-like cell differentiation
Article Snippet: .. The cells were then lysed in 500 µ l modified cell lysis buffer [20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, sodium pyrophosphate, β-glycerophosphate, Na3 VO4 and leupeptin) (Beyotime Institute of Biotechnology) for western blotting and co-IP. .. After lysis, each sample was clarified by centrifugation to remove insoluble debris and the supernatant was preincubated with 20 µ g protein A agarose beads (Beyotime Institute of Biotechnology) for 30 min at 4°C with agitation.

Sonication:

Article Title: Acidic mammalian chitinase gene is highly expressed in the special oxyntic glands of Manis javanica
Article Snippet: .. Immunoblotting Tissue proteins from the three samples were prepared as follows: (a) The tissues were dissected on ice, and the excess blood was wash away; (b) the fresh tissue was placed in a clean mortar and ground in liquid nitrogen; (c) the tissue powder was ground into a precooling tube; (d) an appropriate cell lysis buffer was added, containing 20 mm Tris (pH 7.5), 150 mm NaCl, 1% Triton X‐100, and several protein inhibitors such as sodium pyrophosphate, β‐glycerophosphate, EDTA, Na3 VO4 , and leupeptin (Beyotime Biotechnology, Shanghai, China) according to the amount of tissue (1 mL for 0.1 mg tissue); (e) the supernatant was sonicated on ice for 15–20 min; (f) spin at 12 000 rpm for 15–20 min at 4 °C; and (g) the supernatant was transferred into a new tube that was kept on ice at all times. .. The protein content in the supernatant was quantified using a Bradford protein assay kit (Beyotime Biotechnology).

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    Beyotime na3 vo4
    Na3 Vo4, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Beyotime
    Average 93 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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