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Applichem na3 vo4
Na3 Vo4, supplied by Applichem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na3 vo4/product/Applichem
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na3 vo4 - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Western Blot:

Article Title: Comparison of Cellular Death Pathways after mTHPC-mediated Photodynamic Therapy (PDT) in Five Human Cancer Cell Lines
Article Snippet: .. Western Blotting Cells were collected 6 (LC3-II) or 24 h (PARP, cas 3, LC3-II) after photodynamic treatment, washed twice with PBS and lysed with 100 µL ice-cold lysis buffer, containing 0.1 M NaCl, 0.1 M NaF, 0.2 mM Na3 VO4 (AppliChem, Darmstadt, Germany), 5 mM EDTA, 1% (v /v ) protease inhibitor cocktail (both Sigma-Aldrich, Munich, Germany) and 0.1% (v /v ) Triton X-100 in 50 mM Tris buffer, pH 7.4. .. After ultrasonic treatment for 10 min, cells were centrifuged at 18.000 g at 4 °C for 10 min. For western blot analyses, equal amounts of total protein (10–30 µg) were heated to 95 °C for 5 min in Laemmli buffer [ ], separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Karlsruhe, Germany).

Incubation:

Article Title: Protein kinase casein kinase 2 holoenzyme produced ectopically in human cells can be exported to the external side of the cellular membrane
Article Snippet: .. Cells were then incubated with 1 mg/ml phosvitin (Sigma) in 1.2 ml of buffer P per plate and with 2 mM Na3 VO4 (Applichem, Darmstadt, Germany). .. The cells were incubated with phosvitin for 15 min at 37°C with mild shaking (shedding process).

Lysis:

Article Title: Comparison of Cellular Death Pathways after mTHPC-mediated Photodynamic Therapy (PDT) in Five Human Cancer Cell Lines
Article Snippet: .. Western Blotting Cells were collected 6 (LC3-II) or 24 h (PARP, cas 3, LC3-II) after photodynamic treatment, washed twice with PBS and lysed with 100 µL ice-cold lysis buffer, containing 0.1 M NaCl, 0.1 M NaF, 0.2 mM Na3 VO4 (AppliChem, Darmstadt, Germany), 5 mM EDTA, 1% (v /v ) protease inhibitor cocktail (both Sigma-Aldrich, Munich, Germany) and 0.1% (v /v ) Triton X-100 in 50 mM Tris buffer, pH 7.4. .. After ultrasonic treatment for 10 min, cells were centrifuged at 18.000 g at 4 °C for 10 min. For western blot analyses, equal amounts of total protein (10–30 µg) were heated to 95 °C for 5 min in Laemmli buffer [ ], separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Karlsruhe, Germany).

Protease Inhibitor:

Article Title: Comparison of Cellular Death Pathways after mTHPC-mediated Photodynamic Therapy (PDT) in Five Human Cancer Cell Lines
Article Snippet: .. Western Blotting Cells were collected 6 (LC3-II) or 24 h (PARP, cas 3, LC3-II) after photodynamic treatment, washed twice with PBS and lysed with 100 µL ice-cold lysis buffer, containing 0.1 M NaCl, 0.1 M NaF, 0.2 mM Na3 VO4 (AppliChem, Darmstadt, Germany), 5 mM EDTA, 1% (v /v ) protease inhibitor cocktail (both Sigma-Aldrich, Munich, Germany) and 0.1% (v /v ) Triton X-100 in 50 mM Tris buffer, pH 7.4. .. After ultrasonic treatment for 10 min, cells were centrifuged at 18.000 g at 4 °C for 10 min. For western blot analyses, equal amounts of total protein (10–30 µg) were heated to 95 °C for 5 min in Laemmli buffer [ ], separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Karlsruhe, Germany).

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    Applichem na3 vo4
    Na3 Vo4, supplied by Applichem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na3 vo4/product/Applichem
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na3 vo4 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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