Structured Review

FUJIFILM na deoxycholate
Na Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na deoxycholate/product/FUJIFILM
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
na deoxycholate - by Bioz Stars, 2021-04
86/100 stars

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Western Blot:

Article Title: Generation of kidney tubular organoids from human pluripotent stem cells
Article Snippet: After PBS washes, Alexa Fluor 488- or 594-conjugated antibodies (Invitrogen) and streptavidin-conjugated Alexa Fluor 647 (Invitrogen) were applied for signal detection. .. Western blot analysis Cells were washed twice with PBS and lysed in radioimmunoprecipitation buffer composed of 20 mM Tris-HCl (pH 7.4), 0.1% sodium dodecyl sulphate (SDS), 1% Triton X-100, and 1% Na deoxycholate (all from Wako). .. Protein lysates (20 μg) were resolved by 10% Tris-glycine SDS polyacrylamide gel (Bio-Rad Laboratories, Inc., Hercules, CA, USA) electrophoresis using an Any kD precast gel (Bio Rad Laboratories) and transferred to Immobilon polyvinylidene fluoride membranes (Millipore, Marlborough, MA, USA), which were blocked with Tris-buffered saline containing 5% dried milk (Megmilk Snow Brand Co., Ltd., Hokkaido, Japan).

Article Title: Adhesion signaling by a novel mitotic substrate of src kinases
Article Snippet: .. Immunoblots were performed using mouse monoclonal antibodies for myc and phosphotyrosine (Santa Cruz Biotechnology), Yes (Wako), Src (Calbiochem), rabbit polyclonal antibodies for N-Cadherin & P-Cadherin (Santa Cruz) and MT-SP1 , goat polyclonal for Syndecans 1 & 4 (Santa Cruz). .. PD173955 and PD179483 were obtained from Parke-Davis Pharmaceuticals.

Lysis:

Article Title: NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes
Article Snippet: .. GSCs (∼1 × 106 cells) were gently collected, washed in ice cold PBS before lysing in an equal volume of lysis buffer containing 50 mM Tris pH 7.4, 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl and 1 mM EDTA supplemented with Protease inhibitor cocktail (Roche) and 0.1 mM PMSF (Wako), for 1 h on ice, and centrifuged at 20,000g for 10 min. Supernatant was mixed with 2 × loading buffer and applied to an SDS page gel. .. Horseradish peroxidase conjugated secondary antibodies were from Cell Signaling Technology.

Protease Inhibitor:

Article Title: NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes
Article Snippet: .. GSCs (∼1 × 106 cells) were gently collected, washed in ice cold PBS before lysing in an equal volume of lysis buffer containing 50 mM Tris pH 7.4, 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl and 1 mM EDTA supplemented with Protease inhibitor cocktail (Roche) and 0.1 mM PMSF (Wako), for 1 h on ice, and centrifuged at 20,000g for 10 min. Supernatant was mixed with 2 × loading buffer and applied to an SDS page gel. .. Horseradish peroxidase conjugated secondary antibodies were from Cell Signaling Technology.

SDS Page:

Article Title: NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes
Article Snippet: .. GSCs (∼1 × 106 cells) were gently collected, washed in ice cold PBS before lysing in an equal volume of lysis buffer containing 50 mM Tris pH 7.4, 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl and 1 mM EDTA supplemented with Protease inhibitor cocktail (Roche) and 0.1 mM PMSF (Wako), for 1 h on ice, and centrifuged at 20,000g for 10 min. Supernatant was mixed with 2 × loading buffer and applied to an SDS page gel. .. Horseradish peroxidase conjugated secondary antibodies were from Cell Signaling Technology.

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    FUJIFILM na deoxycholate
    Na Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na deoxycholate/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    na deoxycholate - by Bioz Stars, 2021-04
    86/100 stars
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