Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Transcriptomic data from myxobacteria exposed to C6-AHL. A. Differentially expressed genes and features from M. xanthus exposed to C6-AHL when compared with signal unexposed M. xanthus control ( p ≤ 0.05); * indicates features also impacted at p ≤ 0.01. B. Differentially expressed genes from C. ferrugineus exposed to C6-AHL when compared with signal unexposed C. ferrugineus control ( p ≤ 0.01). Data depicted as an average log 2 fold change from three biological replicates. Impacted features annotated as hypothetical not included.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Putative roles of Prokaryotic Genome Annotation Pipeline (PGAP)-annotated genes impacted by C6-AHL exposure (from ) comparing M. xanthus and C. ferrugineus .

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Transcriptomic data from myxobacteria exposed to HHQ. A. Differentially expressed genes and features from M. xanthus exposed to HHQ when compared with signal unexposed M. xanthus control ( p ≤ 0.05). B. Differentially expressed genes from C. ferrugineus exposed to HHQ when compared with signal unexposed C. ferrugineus control ( p ≤ 0.05). Data depicted as an average log 2 fold change from three biological replicates. Impacted features annotated as hypothetical not included.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Putative roles of PGAP-annotated genes impacted by HHQ exposure (from ) comparing M. xanthus and C. ferrugineus .

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Comparison of metabolomic response to C6-AHL, 3-oxo-C6-AHL and HHQ exposure experiments with M. xanthus (A and B) and C. ferrugineus (C and D). Numbers included in each Venn diagram account for a unique detected feature with a significantly impacted intensity upon exposure to the indicated signalling molecule provided by XCMS-multigroup analysis ( n = 3, p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Comparison of metabolomic response to C6-AHL, 3-oxo-C6-AHL, and HHQ exposure experiments with M. xanthus (A and B) and C. ferrugineus (C and D) including additional C6-AHL + HHQ exposure experiments. Numbers included in each Venn diagram account for a unique detected feature with a significantly impacted intensity upon exposure to the indicated signalling molecule provided by XCMS-multigroup analysis ( n = 3, p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: A. Extracted ion chromatograph (EIC) depicting presence of HQNO and PQS in HHQ exposed extracts from C. ferrugineus and not observed in HHQ exposed extracts from M. xanthus . B. EIC depicting presence of PQS-NO in HHQ exposed extracts of C. ferrugineus , also not present in M. xanthus extracts. Chromatographs rendered with MZmine v2.37. C. Oxidative detoxification of HHQ by C. ferrugineus including exact mass values from ChemDraw Professional v17.1. D. Detected ion intensities for PQS-NO comparing crude extracts of C. ferrugineus exposed to HHQ, PQS and HQNO; detected intensity data provided by XCMS-multigroup analysis ( n = 3; p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: A. Lawn culture predation assay data depicting superior predation of P. aeruginosa by C. ferrugineus ( n = 3; p ≤ 0.005). Statistical significance calculated using an unpaired t test with Welch’s correction. B. Swarming expansion assay data depicting HHQ inhibition of M. xanthus swarming ( n = 4) with significant differences in swarm diameters ( p < 0.001) observed between HHQ exposed and unexposed data after day 3. C. Swarming expansion assay data depicting no significant inhibition of C. ferrugineus swarming ( n = 4) during HHQ exposure conditions. Statistical significance for swarming assays was determined using two-way ANOVA and Tukey’s multiple comparisons test.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques: Inhibition