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Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein <t>to</t> <t>VERO-E6</t> cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.
Mycoplasma Free Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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low passage mycoplasma  (ATCC)
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Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein <t>to</t> <t>VERO-E6</t> cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.
Low Passage Mycoplasma, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycoplasma free hek293 gnti tetr cell lines  (ATCC)
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Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein <t>to</t> <t>VERO-E6</t> cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.
Mycoplasma Free Hek293 Gnti Tetr Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycoplasma free hek293 gntitetr cell lines73  (ATCC)
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ATCC mycoplasma free hek293 gntitetr cell lines73
Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein <t>to</t> <t>VERO-E6</t> cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.
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https://www.bioz.com/result/mycoplasma free hek293 gntitetr cell lines73/product/ATCC
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mycoplasma  (Vazyme Biotech Co)
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Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein <t>to</t> <t>VERO-E6</t> cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.
Mycoplasma, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein to VERO-E6 cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.

Journal: Acta Pharmaceutica Sinica. B

Article Title: A novel fusion protein of COVID-19 virus enhancing protection of Syrian hamsters infected with SARS-CoV-2

doi: 10.1016/j.apsb.2025.11.027

Figure Lengend Snippet: Recombinant fusion protein production and cell-binding ability. (A) Structural features of the SARS-CoV-2 spike protein and the flow chart of protein library construction. The listed domain boundaries are the NTD, N-terminal domain; RBD, receptor-binding domain; FP, fusion peptide; HR1, heptad repeat 1; HR2, heptad repeat 2; TM, transmembrane domain; and CT, cytoplasmic tail. For visual clarity, box lengths are not to scale. (B) Amino acid locations and molecular weights of the protein fragments. (C) Flow cytometry was performed to assess the binding activity of the fusion protein to VERO-E6 cells. Cells were incubated with 0.25 μmol/L His 6 -tagged COVID19-SF2, COVID19-SF5, or COVID19-SF2+SF5. The blue curve represents the cells without added protein. The red curves represent fragment binding. (D) The plasmid encoding the fusion protein was verified by agarose gel electrophoresis following digestion with Bam H I and Nco I. Expression and purification of the protein fragment were analyzed by SDS-PAGE.

Article Snippet: The P1 virus was subsequently passaged at a 1:1000 dilution on mycoplasma-free VERO-E6 cells (ATCC) in DMEM medium (supplemented with 1% penicillin/streptomycin) (Hyclone) and harvested when 80% cytopathic effect (CPE) became evident.

Techniques: Recombinant, Binding Assay, Flow Cytometry, Activity Assay, Incubation, Plasmid Preparation, Agarose Gel Electrophoresis, Expressing, Purification, SDS Page