mycobacterium species  (DSMZ)

Journal: Microorganisms

Article Title: Dysregulated Intestinal Host–Microbe Interactions in Systemic Lupus Erythematosus: Insights from Patients and Mouse Models

doi: 10.3390/microorganisms13030556

Figure Lengend Snippet: Altered microbial abundance in SLE patients relative to healthy controls.

Article Snippet: Clostridium species ATCC BAA-422 , Fecal , Enriched , [ ] .

Techniques:

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Growth measurement over time at an optical density of 600 nm for M. decipiens ( A ), M. lacus ( B ), M. riyadhense ( C ), M. shinjukuense ( D ), and M. kansasii ( E ). Growth was measured at 32°C, 35°C, and 37°C for each strain. Results from two independent experiments in classic culture conditions. Picture of M. decipiens growth at 35°C and 37°C in 7H9 ADC liquid cultures ( F ) and on LJ tubes ( G ) were taken after 10 days of incubation. Comparable quantities of bacteria were used for both temperatures.

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Incubation, Bacteria

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Genome sizes of the four NTM and control strains

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Control, Plasmid Preparation

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Genomic comparisons of ESX loci of M. decipiens with Mtb and M. kansasii . Comparisons of ESX-1 ( A ), ESX-2 ( B ), ESX-3 ( C ), ESX-4 ( D ), and ESX-5 ( E ) were performed using the Artemis Comparison Tool ACT, and the “MicroScope” database from the Genoscope ( https://mage.genoscope.cns.fr/microscope/mage/viewer.php ?).

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Comparison, Microscopy

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Average of amino acid identity of the ESX systems for the four strains compared to Mtb and M. kansasii ^{a b}

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques:

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Western blot-based evaluation of the presence of ESAT-6 and CFP-10 in whole-cell lysate (left panel) and supernatant (right panel) fractions of in vitro cultures of M. decipiens ( M. dec .), M. lacus ( M. lac .), M. riyadhense ( M. riy .), M. shinjukuense ( M. shi .), and Mtb H37Rv that were grown to an OD 600nm of 0.6–0.8 using anti-ESAT-6, anti-CFP-10, as well as anti-Ag85B and anti-SigA control antibodies. An amount of 50 µg of proteins was migrated on SDS-PAGE for 30 min at 200 V and transferred onto a nitrocellulose membrane for 7 min at 110 V ( A ). Western blotting of whole-cell lysate (left panel) and supernatant (right panel) fractions from M. lacus ( M. lac .), M. riyadhense ( M. riy .), M. shinjukuense ( M. shi .), and M. decipiens ( M. dec .) cultures that were grown to an optical density higher than 1 ( B ). Genomic position of the orthologous genes of the espACD cluster that is present in the genome of M. decipiens , compared to the genomic locations of orthologous espACD clusters in selected other mycobacterial species that possess an espACD cluster in their genomes ( C ). Note that the orthologous flanking genes of the espACD cluster identified in the various species refer to the Mtb H37Rv gene nomenclature and relative genomic localization. Image adapted from reference with permission and complemented with new information on the M. decipiens espACD cluster. Genes depicted as white arrows correspond to M. decipiens specific genes.

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Western Blot, In Vitro, Control, SDS Page, Membrane

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Determination of MIC range by resazurin assay ^a

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Resazurin Assay

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Infection of THP-1-derived macrophages determined by CFU counting at 0, 3, 5, and 7 days after infection. Results represent between three and six independent experiments at 35°C and 37°C for M. decipiens ( A ), M. lacus ( B ), M. riyadhense ( C ), M. shinjukuense ( D ), M. kansasii ( E ), M. bovis BCG Pasteur strain ( F ), and Mtb H37Rv ( G ). Data are represented as means and SEM of three to six biological experiments, each experiments containing three technical replicates. Statistical differences in CFU were determined by a two-way ANOVA test with Dunnett’s multiple comparisons test (* P < 0.033; ** P < 0.002; *** P < 0.001; ns, non-significant).

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: Infection, Derivative Assay

Journal: Microbiology Spectrum

Article Title: Genomic and phenotypic characterization of Mycobacterium tuberculosis’ closest-related non-tuberculous mycobacteria

doi: 10.1128/spectrum.04126-23

Figure Lengend Snippet: Evaluation of in vivo growth capacity of M. decipiens , M. lacus , M. riyadhense , M. shinjukuense , M. kansasii , M. bovis BCG Pasteur, and Mtb H37Rv in two murine models. CFU counts in control mice at day 1 post-infection and titration of aerosolized culture of both experiments are indicated (A and E). Results from C3HeB/FeJ infection and ratios of different time points vs day 1 counts are indicated (B). Results from C3HeB/FeJ infection based on CFU counts at different time points. Results are shown at a log 10 scale for lungs (C) and spleens (D). Same types of results are presented for the C57BL/6J infection with ratios of CFUs D1/CFUs titration (F), CFU counts in lungs (G) and spleens (H). Each experiment was performed once, and the represented values correspond to the number of mice at each time point. "ND": not detected.

Article Snippet: Reference strains belonging to the species M. decipiens (ATCC TSD-117), M. lacus (DSM 44577), M. riyadhense (DSM 45176), and M. shinjukuense (DSM 45663) were purchased from the American Type Culture collection (ATCC) and German Collection of Microorganisms and Cell Cultures (DSM), respectively.

Techniques: In Vivo, Control, Infection, Titration

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: (a) The figure shows the zone of inhibition of PEG-AgNPs, AgNPs, PLE, and AgNO 3 with Linezolid taken as a standard drug against the three Mycobacterium species i.e., (1) M. smegmatis (2) M. fortuitum, and (3) M. marinum. (b) Hemolytic activity of PEG-AgNPs (100 μg/mL), AgNPs (100 μg/mL), PLE (500 mg/mL), Linezolid (100 μg/mL), AgNO 3 (1 mM), and Triton X-100 (0.5 % v/v) on human blood agar. Triton X-100 and Linezolid were taken as positive control. (c) Flow cytometric analysis to determine the membrane damage of (1) M. smegmatis (2) M. fortuitum, and (3) M. marinum . (i–x) PI staining of all three Mycobacterium species (i) control without PI treatment, (ii) control with PI treatment, (iii) Linezolid, (iv) PEG-AgNPs (1X), (v) PEG-AgNPs (2X), (vi) Plant leaves extract, (vii) AgNPs (1X), (viii) AgNPs (2X), (ix) AgNO 3 , and (x) Triton X-100. Linezolid and Triton X-100 were taken as positive controls. In each quadrant, Q 1 and Q 3 depict the percentage of live cells whereas Q 2 and Q 4 depict the percentage of dead cells. (d) Bar diagram representing the percentage of dead cells of Mycobacterium species treated with test compounds.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: Inhibition, Activity Assay, Positive Control, Membrane, Staining

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: Zone of inhibition and the MIC values of PEG-AgNPs, AgNPs, and PLE against Mycobacterium species.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: Inhibition, Concentration Assay

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: Topographic statistics of biofilm surfaces analyzed through AFM.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: