murine moloney leukaemia virus reverse transcriptase  (PerkinElmer)

 
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    Structured Review

    PerkinElmer murine moloney leukaemia virus reverse transcriptase
    Murine Moloney Leukaemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine moloney leukaemia virus reverse transcriptase/product/PerkinElmer
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    murine moloney leukaemia virus reverse transcriptase - by Bioz Stars, 2020-08
    88/100 stars

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    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Potential role of phosphodiesterase 7 in human T cell function: comparative effects of two phosphodiesterase inhibitors
    Article Snippet: .. Dermatophagoides farinae mite extract ( Df ; Torii Pharmaceutical Co., Tokyo, Japan), anti-CD3 monoclonal antibody (MoAb; Ortho, Raritan, NJ, USA), purified rat antimouse/human IL-5 monoclonal antibody (Pharmingen, San Diego, CA, USA), biotinylated rat antihuman IL-5 monoclonal antibody (Pharmingen), Cell Titer 96™ AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI, USA), ISOGEN (Nippongene, Tokyo, Japan), murine Moloney leukaemia virus reverse transcriptase (Perkin-Elmer Cetus, Norwalk, CT, USA), GeneAmp® DNA polymerase (Perkin-Elmer Cetus), RT-PCR Amplimer Sets (Clontech, Palo Alto, CA, USA), fluorescein isocyanate (FITC)-labelled anti-CD25 antibody (Becton Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-labelled anti-CD3 antibody (Becton Dickinson), Ficoll-Paque (Pharmacia, Uppsala, Sweden) and AIM-V medium (G ibco BRL, Gaithersburg, MD, USA) were used. ..

    Purification:

    Article Title: Potential role of phosphodiesterase 7 in human T cell function: comparative effects of two phosphodiesterase inhibitors
    Article Snippet: .. Dermatophagoides farinae mite extract ( Df ; Torii Pharmaceutical Co., Tokyo, Japan), anti-CD3 monoclonal antibody (MoAb; Ortho, Raritan, NJ, USA), purified rat antimouse/human IL-5 monoclonal antibody (Pharmingen, San Diego, CA, USA), biotinylated rat antihuman IL-5 monoclonal antibody (Pharmingen), Cell Titer 96™ AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI, USA), ISOGEN (Nippongene, Tokyo, Japan), murine Moloney leukaemia virus reverse transcriptase (Perkin-Elmer Cetus, Norwalk, CT, USA), GeneAmp® DNA polymerase (Perkin-Elmer Cetus), RT-PCR Amplimer Sets (Clontech, Palo Alto, CA, USA), fluorescein isocyanate (FITC)-labelled anti-CD25 antibody (Becton Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-labelled anti-CD3 antibody (Becton Dickinson), Ficoll-Paque (Pharmacia, Uppsala, Sweden) and AIM-V medium (G ibco BRL, Gaithersburg, MD, USA) were used. ..

    Article Title: Cyclic AMP suppresses interleukin-5 synthesis by human helper T cells via the downregulation of the calcium mobilization pathway
    Article Snippet: .. PGE2 , forskolin and dibutyryl (db)-cAMP were from Sigma (St. Louis, MO, U.S.A.), rhIL-2 was from Peprotech (London, U.K.), OKT3 was from Ortho (Raritan, NJ, U.S.A.), purified rat anti-mouse/human IL-5 monoclonal antibody, biotinylated rat anti-human IL-5 monoclonal antibody, PE-labelled anti-CD25 antibody, and its isotype-matched control antibody were from Pharmingen (San Diego, CA, U.S.A.), anti-mouse IgG antibody was from Organon Technica (Durham, NC, U.S.A.), [γ-32 P]-ATP was from Amersham (Buckinghamshire, U.K.), acetoxymethyl-Fura-2 was from Dojindo (Kumamoto, Japan), ISOGEN was from Nippongene (Tokyo, Japan), murine Moloney leukaemia virus reverse transcriptase and GeneAmp® DNA polymerase were from Perkin-Elmer Cetus (Norwalk, CT, U.S.A.), T4 polynucleotide kinase was from Takara (Otsu, Japan), RX film was from Fuji Photo Film (Tokyo, Japan), Ficoll-Paque was from Pharmacia (Uppsala, Sweden), and AIM-V and RPMI1640 medium were from Gibco BRL (Gaithersburg, MD, U.S.A.). ..

    Proliferation Assay:

    Article Title: Potential role of phosphodiesterase 7 in human T cell function: comparative effects of two phosphodiesterase inhibitors
    Article Snippet: .. Dermatophagoides farinae mite extract ( Df ; Torii Pharmaceutical Co., Tokyo, Japan), anti-CD3 monoclonal antibody (MoAb; Ortho, Raritan, NJ, USA), purified rat antimouse/human IL-5 monoclonal antibody (Pharmingen, San Diego, CA, USA), biotinylated rat antihuman IL-5 monoclonal antibody (Pharmingen), Cell Titer 96™ AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI, USA), ISOGEN (Nippongene, Tokyo, Japan), murine Moloney leukaemia virus reverse transcriptase (Perkin-Elmer Cetus, Norwalk, CT, USA), GeneAmp® DNA polymerase (Perkin-Elmer Cetus), RT-PCR Amplimer Sets (Clontech, Palo Alto, CA, USA), fluorescein isocyanate (FITC)-labelled anti-CD25 antibody (Becton Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-labelled anti-CD3 antibody (Becton Dickinson), Ficoll-Paque (Pharmacia, Uppsala, Sweden) and AIM-V medium (G ibco BRL, Gaithersburg, MD, USA) were used. ..

    Synthesized:

    Article Title: Potential role of phosphodiesterase 7 in human T cell function: comparative effects of two phosphodiesterase inhibitors
    Article Snippet: .. Briefly, RNA was extracted from the pelleted cells essentially following the one-step acid guanidinium isothiocyanate/phenol chloroform extraction method [ ] using ISOGEN. cDNA was synthesized from 1 μg cytoplasmic RNA using random primers and murine Moloney leukaemia virus reverse transcriptase. .. PCR was performed using the following RT-PCR amplimer sets.

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    PerkinElmer mulv
    Mulv, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mulv/product/PerkinElmer
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    mulv - by Bioz Stars, 2020-08
    91/100 stars
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    92
    PerkinElmer moloney murine leukaemia virus reverse transcriptase
    Moloney Murine Leukaemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    PerkinElmer moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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