multisite gateway pdonr221 plasmid set  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multisite gateway pdonr221 plasmid set
    Multisite Gateway Pdonr221 Plasmid Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multisite gateway pdonr221 plasmid set/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multisite gateway pdonr221 plasmid set - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Multiplexing:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations. .. 2.4 Cas9/sgRNA destination and expression constructs To generate the three destination vectors, we purified a fragment containing the Cas9 and Gateway cassette from pH‐Ubi‐Cas9 (Miao et al., ) digested with StuI and PmeI .

    Clone Assay:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: Positive clones were digested with AscI and SalI , and the dropout was subsequently ligated into an AscI ‐ and SalI ‐linearized pENTR‐PpU6‐sgRNA‐NGG plasmid. .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations.

    Amplification:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations. .. 2.4 Cas9/sgRNA destination and expression constructs To generate the three destination vectors, we purified a fragment containing the Cas9 and Gateway cassette from pH‐Ubi‐Cas9 (Miao et al., ) digested with StuI and PmeI .

    Construct:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: Paragraph title: U6 promoter/sgRNA entry vector constructs ... To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations.

    Expressing:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations. .. 2.4 Cas9/sgRNA destination and expression constructs To generate the three destination vectors, we purified a fragment containing the Cas9 and Gateway cassette from pH‐Ubi‐Cas9 (Miao et al., ) digested with StuI and PmeI .

    Polymerase Chain Reaction:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: The two fragments were then ligated using an overlap extension PCR and ligated into pGEM/T‐Easy (Promega). .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations.

    Plasmid Preparation:

    Article Title: Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens. Efficient and modular CRISPR‐Cas9 vector system for Physcomitrella patens
    Article Snippet: .. To generate the six entry vectors compatible with Multisite Gateway (Invitrogen) for multiplexing experiments, we amplified the sgRNA expression cassette from pENTR‐PpU6‐sgRNA‐L1L2 using primers (Table ) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer's recommendations. .. 2.4 Cas9/sgRNA destination and expression constructs To generate the three destination vectors, we purified a fragment containing the Cas9 and Gateway cassette from pH‐Ubi‐Cas9 (Miao et al., ) digested with StuI and PmeI .

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    Thermo Fisher multisite gateway pro pdonr221 plasmid
    Multisite Gateway Pro Pdonr221 Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multisite gateway pro pdonr221 plasmid/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multisite gateway pro pdonr221 plasmid - by Bioz Stars, 2020-04
    95/100 stars
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