multiple pyk2 sirna oligonucleotides  (Millipore)


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    Structured Review

    Millipore multiple pyk2 sirna oligonucleotides
    Knockdown of <t>Pyk2</t> with <t>siRNA</t> inhibits EGFR activation after ATP stimulation but not after stimulation with EGF. (A) HCLE cells transfected with 10 nM of control or Pyk2 siRNA were treated with ATP as indicated. Immunoblots were probed for EGFR phosphorylated
    Multiple Pyk2 Sirna Oligonucleotides, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiple pyk2 sirna oligonucleotides/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    multiple pyk2 sirna oligonucleotides - by Bioz Stars, 2020-04
    85/100 stars

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    Images

    1) Product Images from "Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor"

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor

    Journal: Cellular signalling

    doi: 10.1016/j.cellsig.2011.07.021

    Knockdown of Pyk2 with siRNA inhibits EGFR activation after ATP stimulation but not after stimulation with EGF. (A) HCLE cells transfected with 10 nM of control or Pyk2 siRNA were treated with ATP as indicated. Immunoblots were probed for EGFR phosphorylated
    Figure Legend Snippet: Knockdown of Pyk2 with siRNA inhibits EGFR activation after ATP stimulation but not after stimulation with EGF. (A) HCLE cells transfected with 10 nM of control or Pyk2 siRNA were treated with ATP as indicated. Immunoblots were probed for EGFR phosphorylated

    Techniques Used: Activation Assay, Transfection, Western Blot

    2) Product Images from "Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *"

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.083089

    Pyk2 activation is necessary for full induction of cell motility. A , HCLE cells were transfected with control or Pyk2 siRNA or were infected with control or PRNK-expressing adenovirus prior to wound healing assays in the presence of 25 units/ml apyrase and 10 ng/ml EGF (EGF) as indicated. The percentage change in healing from corresponding untreated or EGF-treated control was plotted. *, p
    Figure Legend Snippet: Pyk2 activation is necessary for full induction of cell motility. A , HCLE cells were transfected with control or Pyk2 siRNA or were infected with control or PRNK-expressing adenovirus prior to wound healing assays in the presence of 25 units/ml apyrase and 10 ng/ml EGF (EGF) as indicated. The percentage change in healing from corresponding untreated or EGF-treated control was plotted. *, p

    Techniques Used: Activation Assay, Transfection, Infection, Expressing

    Related Articles

    Sequencing:

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor
    Article Snippet: .. Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. As a control siRNA, MISSION® siRNA Universal Negative Control #1 (Sigma) was used.

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *
    Article Snippet: .. Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. As a control siRNA, we used MISSION® siRNA Universal Negative Control 1 (Sigma).

    Clone Assay:

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor
    Article Snippet: Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. Pyk2 and PRNK cloned into adenovirus vectors were from Dr. Joseph C. Loftus (Mayo Clinic, Scottsdale, AZ).

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *
    Article Snippet: Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. FLAG-tagged Pyk2 and FLAG-tagged PRNK cloned into adenovirus vectors were from Dr. Joseph C. Loftus (Mayo Clinic, Scottsdale, AZ).

    Transfection:

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor
    Article Snippet: Paragraph title: 2.4 siRNA transfection and adenoviral infection ... Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore).

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *
    Article Snippet: Paragraph title: siRNA Transfection and Adenoviral Infection ... Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore).

    Negative Control:

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor
    Article Snippet: Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. As a control siRNA, MISSION® siRNA Universal Negative Control #1 (Sigma) was used.

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *
    Article Snippet: Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore). .. As a control siRNA, we used MISSION® siRNA Universal Negative Control 1 (Sigma).

    Infection:

    Article Title: Extracellular ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor
    Article Snippet: Paragraph title: 2.4 siRNA transfection and adenoviral infection ... Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore).

    Article Title: Pyk2 Activation Triggers Epidermal Growth Factor Receptor Signaling and Cell Motility after Wounding Sheets of Epithelial Cells *
    Article Snippet: Paragraph title: siRNA Transfection and Adenoviral Infection ... Multiple Pyk2 siRNA oligonucleotides were used: CACAUGAAGUCCGAUGAGAdTdT (Sigma) and a Pyk2 SMART pool that contains at least four duplexes of undisclosed sequence (Millipore).