Journal: Neural Regeneration Research

Article Title: Gut microbiota dysbiosis contributes to α-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson's disease

doi: 10.4103/1673-5374.391191

Figure Lengend Snippet: Rotenone administration induces intestinal barrier disruption in a gut microbiota-dependent manner. (A) Colonic mucus layer thickness (per section/two sections per animal, n = 3). (B) Alcian blue-stained colonic sections showing the mucus layer (arrows). The paired black arrows indicate where the mucus layer was measured. Scale bars: 50 μm ( n = 3). (C) Immunofluorescence images of colonic sections stained with anti-MUC2 antibody and 4′,6-diamidino-2-phenylindole (DAPI). Paired white arrows indicate the mucus layer. Scale bars: 50 μm. (D) Protein levels of occludin and zonula occludens-1 (ZO-1) in the colon ( n = 5). (E) Serum lipopolysaccharide (LPS) endotoxin levels ( n = 10). (F–H) mRNA expression levels of interleukin (IL-6; F), tumor necrosis factor-α (TNF-α; G), and inducible nitric oxide synthase (iNOS; H) in the colon ( n = 5). (I) Colon length ( n = 9), with representative images shown. (J) Colonic mucus layer thickness (per section/two sections per animal, n = 3). (K) Alcian blue-stained colonic sections showing the mucus layer (arrows). Paired black arrows indicate where the mucus layer was measured (scale bars: 50 μm). (L) Immunofluorescence images of colonic sections stained with anti-MUC2 antibody and DAPI. Paired white arrows indicate the mucus layer. Scale bars: 50 μm. (M) Protein levels of occludin and ZO-1 in the colon ( n = 5). (N) Serum LPS endotoxin levels ( n = 10). (O–Q) mRNA expression levels of IL-6, TNF-α, and iNOS in the colon ( n = 5). (R) Colon length ( n = 9), with representative images shown. Values are presented as mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, vs . Con. Con: control; Rot: rotenone; Con + Ab: Con mice treated with antibiotics; Rot + Ab: Rot mice treated with antibiotics.

Article Snippet: The sections were cross-validated with primary antibody anti-mucin-2 (MUC2) (rabbit, 1:500, Abclonal, Cat# A14659) staining at 4°C overnight, followed by incubation with goat-anti-rabbit IgG H&L/AF594 antibody (goat, 1:1000, Bioss, Beijng, China, Cat# bs-0295G-AF594) for 1 hour at room temperature.

Techniques: Disruption, Staining, Immunofluorescence, Expressing

Journal: Neural Regeneration Research

Article Title: Gut microbiota dysbiosis contributes to α-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson's disease

doi: 10.4103/1673-5374.391191

Figure Lengend Snippet: Transplantation of healthy gut microbiota alleviates rotenone-induced motor deficits, intestinal inflammation, endotoxemia, and intestinal barrier impairment. (A) Rotarod test. The Y-axis shows the time spent on the rotarod. (B) Pole test. The Y-axis shows the time of descent from the top of the pole to the ground. (C) Colon length ( n = 9), with representative images shown. (D–F) mRNA expression levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) in the colon ( n = 5). (G) Serum lipopolysaccharide (LPS) endotoxin levels ( n = 10). (H) Colonic mucus layer thickness (per section/two sections per animal, n = 3). (I) Alcian blue-stained colonic sections showing the mucus layer (arrows). Paired black arrows show where the mucus layer was measured (scale bars: 50 μm). (J) Immunofluorescence images of colonic sections stained with anti-mucin-2 (MUC2) antibody and 4′,6-diamidino-2-phenylindole (DAPI). Paired white arrows indicate the mucus layer. Scale bars: 50 μm. Values are mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, vs. Rot + PBS. Rot: Rotenone; Rot + FMT Con : Rot mice transplanted with gut microbiota from donor control mice; Rot + PBS: Rot mice treated with PBS. PBS: Phosphate-buffered saline.

Article Snippet: The sections were cross-validated with primary antibody anti-mucin-2 (MUC2) (rabbit, 1:500, Abclonal, Cat# A14659) staining at 4°C overnight, followed by incubation with goat-anti-rabbit IgG H&L/AF594 antibody (goat, 1:1000, Bioss, Beijng, China, Cat# bs-0295G-AF594) for 1 hour at room temperature.

Techniques: Transplantation Assay, Expressing, Staining, Immunofluorescence, Saline

Journal: Cancers

Article Title: Molecular Characterization and Xenotransplantation of Pancreatic Cancer Using Endoscopic Ultrasound-Guided Fine Needle Aspiration (EUS-FNA)

doi: 10.3390/cancers16152721

Figure Lengend Snippet: Histology and immunohistochemical analysis of patient-derived xenografts. Hematoxylin and eosin staining of several xenograft samples and IHC images of tumor blocks stained with cytokeratin 18, E-Cadherin, mucin 2 and 4 and smooth muscle actin. Row labels denote sample designation. Scale bars are equal to 100 µm.

Article Snippet: Tumor sections were stained against cytokeratin 18 (epithelial tumor cell marker, overexpressed in most adenocarcinomas) [ ] (1:1000, ab55395), E-Cadherin (epithelial to mesenchymal transition marker, downregulated in advanced carcinomas) [ , ] (1:1000, ab15148), mucin 2 (intraductal papillary mucinous neoplasm cell marker, not expressed in other types of pancreatic ductal adenocarcinoma precursors) [ ] (1:500, ab76774), mucin 4 (pancreatic tumor cell marker, overexpressed in pancreatic tumors) [ ] (1:1000, Sigma-HPA005895) and smooth muscle actin (fibroblast marker) (1:200, ab5694).

Techniques: Immunohistochemical staining, Derivative Assay, Staining