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TaKaRa expand taq
Expand Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expand taq/product/TaKaRa
Average 90 stars, based on 5356 article reviews
Price from $9.99 to $1999.99
expand taq - by Bioz Stars, 2020-09
90/100 stars

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Countercurrent Chromatography:

Article Title: Extracellular Vesicles in Luminal Fluid of the Ovine Uterus
Article Snippet: .. The cDNA was used in subsequent PCR reactions with TaKaRa hot start DNA polymerase (Clontech Laboratories, Inc., Mountain View, CA) and primers designed to target enJSRVs env (forward: GRY TTT CCR TGG GAT AAG GTG AA , reverse: ACA ATC ACY AGA CCC TTA CCA TTG ) and gag (forward: GTG CTG ACA TTG GAC CCT , reverse: CAC AAA CAA AGC AAC CCG AA ) RNA under the following conditions: 95°C for 2 minutes; 35 cycles of 98°C for 10 seconds, 55°C for 30 seconds, 72°C for 1 minute; and a final step of 72°C for 2 minutes. .. The PCR products were separated in a 1% agarose in TAE gel containing ethidium bromide and visualized using a ChemiDoc MP system and Image Lab 4.1 software (BioRad).

Amplification:

Article Title: Combined effects of FH (E404D) and ACOX2 (R409H) cause metabolic defects in primary cardiac malignant tumor
Article Snippet: .. PCRs were amplified by using TaqTM HotStart DNA Polymerase (TaKaRa Bio Inc., Shiga, Japan). .. PCR products were purified by Exon/SAP and sequenced by the BigDye Cycle Sequencing Kit (Thermo Fisher Scientific, Waltham, MA, USA) and ABI 3730 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).

Article Title: Loss-of-function mutations in MGME1 impair mtDNA replication and cause multi-systemic mitochondrial disease
Article Snippet: .. For the amplification, TaKaRa LA Taq Hot Start DNA polymerase (Takara Bio Inc., Otsu, Japan) was used under the following conditions: 95°C for 2.5 min, 10 cycles of 92°C for 20 s and 68°C for 14 min, 20 cycles of 92°C for 25 s and 68°C for 16 min, and 72°C for 10 min. To quantify deletion levels, a single-molecule PCR approach was used as described previously . .. Briefly, single mtDNA deletions were amplified using the same conditions as in long-range PCR, but in 42 cycles of PCR.

Article Title: A GSDMB enhancer-driven HSV thymidine kinase-expressing vector for controlling occult peritoneal dissemination of gastric cancer cells
Article Snippet: .. Reporter Assay A genomic fragment, from −1080 to +1053 of GSDMB and containing the LTR promoter, was amplified by PCR using LA Taq Hot Start DNA polymerase (Takara) in 35 cycles of 96 °C for 30 s and 68 °C for 2 min, using primer sets: 5′-CTTCCTGAGATTCAGAGGCC-3′ and 5′-CTCGAGTTCACTGTGTTAGCCAGG-3′, and inserted into a pGL3 basic vector (Promega, Madison, WI). ..

Reporter Assay:

Article Title: A GSDMB enhancer-driven HSV thymidine kinase-expressing vector for controlling occult peritoneal dissemination of gastric cancer cells
Article Snippet: .. Reporter Assay A genomic fragment, from −1080 to +1053 of GSDMB and containing the LTR promoter, was amplified by PCR using LA Taq Hot Start DNA polymerase (Takara) in 35 cycles of 96 °C for 30 s and 68 °C for 2 min, using primer sets: 5′-CTTCCTGAGATTCAGAGGCC-3′ and 5′-CTCGAGTTCACTGTGTTAGCCAGG-3′, and inserted into a pGL3 basic vector (Promega, Madison, WI). ..

Quantitative RT-PCR:

Article Title: High-Risk Human Papillomavirus E7 Maintains Stemness Via APH1B In Cervical Cancer Stem-Cell Like Cells
Article Snippet: .. Evaluation of target factor mRNA levels was performed using the SYBR Premix Ex Taq Kits (#RR042A TaKaRa) for qRT-PCR analysis. ..

Article Title: The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer
Article Snippet: .. Evaluation of CHFR mRNA level was performed by using SYBR Premix Ex Taq Kits (#RR042A TaKaRa) analyzed by qRT-PCR. ..

Polymerase Chain Reaction:

Article Title: Loss-of-function mutations in MGME1 impair mtDNA replication and cause multi-systemic mitochondrial disease
Article Snippet: .. For the amplification, TaKaRa LA Taq Hot Start DNA polymerase (Takara Bio Inc., Otsu, Japan) was used under the following conditions: 95°C for 2.5 min, 10 cycles of 92°C for 20 s and 68°C for 14 min, 20 cycles of 92°C for 25 s and 68°C for 16 min, and 72°C for 10 min. To quantify deletion levels, a single-molecule PCR approach was used as described previously . .. Briefly, single mtDNA deletions were amplified using the same conditions as in long-range PCR, but in 42 cycles of PCR.

Article Title: Extracellular Vesicles in Luminal Fluid of the Ovine Uterus
Article Snippet: .. The cDNA was used in subsequent PCR reactions with TaKaRa hot start DNA polymerase (Clontech Laboratories, Inc., Mountain View, CA) and primers designed to target enJSRVs env (forward: GRY TTT CCR TGG GAT AAG GTG AA , reverse: ACA ATC ACY AGA CCC TTA CCA TTG ) and gag (forward: GTG CTG ACA TTG GAC CCT , reverse: CAC AAA CAA AGC AAC CCG AA ) RNA under the following conditions: 95°C for 2 minutes; 35 cycles of 98°C for 10 seconds, 55°C for 30 seconds, 72°C for 1 minute; and a final step of 72°C for 2 minutes. .. The PCR products were separated in a 1% agarose in TAE gel containing ethidium bromide and visualized using a ChemiDoc MP system and Image Lab 4.1 software (BioRad).

Article Title: A GSDMB enhancer-driven HSV thymidine kinase-expressing vector for controlling occult peritoneal dissemination of gastric cancer cells
Article Snippet: .. Reporter Assay A genomic fragment, from −1080 to +1053 of GSDMB and containing the LTR promoter, was amplified by PCR using LA Taq Hot Start DNA polymerase (Takara) in 35 cycles of 96 °C for 30 s and 68 °C for 2 min, using primer sets: 5′-CTTCCTGAGATTCAGAGGCC-3′ and 5′-CTCGAGTTCACTGTGTTAGCCAGG-3′, and inserted into a pGL3 basic vector (Promega, Madison, WI). ..

CTG Assay:

Article Title: Extracellular Vesicles in Luminal Fluid of the Ovine Uterus
Article Snippet: .. The cDNA was used in subsequent PCR reactions with TaKaRa hot start DNA polymerase (Clontech Laboratories, Inc., Mountain View, CA) and primers designed to target enJSRVs env (forward: GRY TTT CCR TGG GAT AAG GTG AA , reverse: ACA ATC ACY AGA CCC TTA CCA TTG ) and gag (forward: GTG CTG ACA TTG GAC CCT , reverse: CAC AAA CAA AGC AAC CCG AA ) RNA under the following conditions: 95°C for 2 minutes; 35 cycles of 98°C for 10 seconds, 55°C for 30 seconds, 72°C for 1 minute; and a final step of 72°C for 2 minutes. .. The PCR products were separated in a 1% agarose in TAE gel containing ethidium bromide and visualized using a ChemiDoc MP system and Image Lab 4.1 software (BioRad).

Cellular Antioxidant Activity Assay:

Article Title: Extracellular Vesicles in Luminal Fluid of the Ovine Uterus
Article Snippet: .. The cDNA was used in subsequent PCR reactions with TaKaRa hot start DNA polymerase (Clontech Laboratories, Inc., Mountain View, CA) and primers designed to target enJSRVs env (forward: GRY TTT CCR TGG GAT AAG GTG AA , reverse: ACA ATC ACY AGA CCC TTA CCA TTG ) and gag (forward: GTG CTG ACA TTG GAC CCT , reverse: CAC AAA CAA AGC AAC CCG AA ) RNA under the following conditions: 95°C for 2 minutes; 35 cycles of 98°C for 10 seconds, 55°C for 30 seconds, 72°C for 1 minute; and a final step of 72°C for 2 minutes. .. The PCR products were separated in a 1% agarose in TAE gel containing ethidium bromide and visualized using a ChemiDoc MP system and Image Lab 4.1 software (BioRad).

Plasmid Preparation:

Article Title: A GSDMB enhancer-driven HSV thymidine kinase-expressing vector for controlling occult peritoneal dissemination of gastric cancer cells
Article Snippet: .. Reporter Assay A genomic fragment, from −1080 to +1053 of GSDMB and containing the LTR promoter, was amplified by PCR using LA Taq Hot Start DNA polymerase (Takara) in 35 cycles of 96 °C for 30 s and 68 °C for 2 min, using primer sets: 5′-CTTCCTGAGATTCAGAGGCC-3′ and 5′-CTCGAGTTCACTGTGTTAGCCAGG-3′, and inserted into a pGL3 basic vector (Promega, Madison, WI). ..

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    TaKaRa mtdna deletions long range pcr
    Mtdna Deletions Long Range Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtdna deletions long range pcr/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mtdna deletions long range pcr - by Bioz Stars, 2020-09
    85/100 stars
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