msu preparation  (Millipore)


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    Structured Review

    Millipore msu preparation
    Msu Preparation, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msu preparation/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    msu preparation - by Bioz Stars, 2020-09
    88/100 stars

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    gout animal model msu crystals

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    Animal Model:

    Article Title: Effects of RuPeng15 Powder (RPP15) on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats
    Article Snippet: .. MSU Preparation and the Gout Animal Model MSU crystals (from sigma) were sterilized by heating at 180°C for 2 h before experiments. .. Rats were anesthetized with intraperitoneal injection at a dose of urethane (1.0 g/kg), and MSU crystals (20 mg/mL) were injected into the synovial space of both sides of the knee joint in a volume of 50 uL sterile phosphate buffered saline (PBS) before the injection the suspensions was vortexed vigorously [ ].

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  • 92
    Millipore pg cell msu crystals
    Aggregation of neutrophils stimulated with triggers of NET formation. A ) Representative photographs from high-density (1 <t>×</t> 10 8 cells/ml) cultures of human peripheral blood neutrophils incubated with <t>MSU</t> crystals, PMA, Iono, or without stimulants for 3 h. Scale bar, 3 mm. B ) Scanning electron microscope micrographs from high-density (1 × 10 8 cells/ml) neutrophil cultures stimulated as indicated. Scale bars, 20 µm. C ) Representative multiphoton images of neutrophils stained with DNA dye SiR-DNA and membrane marker PKH67 and cultured in densities of 1 × 10 8 cells/ml with MSU crystals or without stimulant. Scale bars, 50 µm. D ) Assessment of MSU crystal–induced aggNETs with combination of multiphoton imaging and polarization microscopy. Scale bar, 50 µm. All experiments were performed independently 3 times.
    Pg Cell Msu Crystals, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pg cell msu crystals/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pg cell msu crystals - by Bioz Stars, 2020-09
    92/100 stars
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    89
    Millipore nigericin sodium salt
    LTB 4 -induced BTK phosphorylation is required for inflammasome assembly. Macrophages were stimulated with 100 ng/mL LPS for 3h, followed by treatment with BTK inhibitor (Ibrutinib), PI3K inhibitor (wortmanin), Syk inhibitor (R406) and PKCδ (Rottlerin) for 20 min and 10nM LTB 4 for 10 min and 1uM <t>nigericin</t> treatment for 1h. A) IL-1β quantification by ELISA. B) Western blotting analysis of caspase-1 activation. The numbers represent mean densitometric analysis of the bands shown from 3 independent experiments. C) WT and BLT1 −/− mice were infected with MRSA for 24 h and skin lysates from WT or BLT1 −/− mice were subjected to immunoblotting, followed by detection of total and phosphorylated BTK (Tyr223). D) PLA of pBTK (Tyr223) and ASC in the infected skin of WT topically with vehicle control or LTB 4 . Data are the mean ± SEM of 5 mice from at least 2 individual experiments. *p
    Nigericin Sodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nigericin sodium salt/product/Millipore
    Average 89 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    nigericin sodium salt - by Bioz Stars, 2020-09
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    91
    Millipore msu
    LTB 4 -induced BTK phosphorylation is required for inflammasome assembly. Macrophages were stimulated with 100 ng/mL LPS for 3h, followed by treatment with BTK inhibitor (Ibrutinib), PI3K inhibitor (wortmanin), Syk inhibitor (R406) and PKCδ (Rottlerin) for 20 min and 10nM LTB 4 for 10 min and 1uM <t>nigericin</t> treatment for 1h. A) IL-1β quantification by ELISA. B) Western blotting analysis of caspase-1 activation. The numbers represent mean densitometric analysis of the bands shown from 3 independent experiments. C) WT and BLT1 −/− mice were infected with MRSA for 24 h and skin lysates from WT or BLT1 −/− mice were subjected to immunoblotting, followed by detection of total and phosphorylated BTK (Tyr223). D) PLA of pBTK (Tyr223) and ASC in the infected skin of WT topically with vehicle control or LTB 4 . Data are the mean ± SEM of 5 mice from at least 2 individual experiments. *p
    Msu, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msu/product/Millipore
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    msu - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Aggregation of neutrophils stimulated with triggers of NET formation. A ) Representative photographs from high-density (1 × 10 8 cells/ml) cultures of human peripheral blood neutrophils incubated with MSU crystals, PMA, Iono, or without stimulants for 3 h. Scale bar, 3 mm. B ) Scanning electron microscope micrographs from high-density (1 × 10 8 cells/ml) neutrophil cultures stimulated as indicated. Scale bars, 20 µm. C ) Representative multiphoton images of neutrophils stained with DNA dye SiR-DNA and membrane marker PKH67 and cultured in densities of 1 × 10 8 cells/ml with MSU crystals or without stimulant. Scale bars, 50 µm. D ) Assessment of MSU crystal–induced aggNETs with combination of multiphoton imaging and polarization microscopy. Scale bar, 50 µm. All experiments were performed independently 3 times.

    Journal: The FASEB Journal

    Article Title: Aggregated neutrophil extracellular traps resolve inflammation by proteolysis of cytokines and chemokines and protection from antiproteases

    doi: 10.1096/fj.201800752R

    Figure Lengend Snippet: Aggregation of neutrophils stimulated with triggers of NET formation. A ) Representative photographs from high-density (1 × 10 8 cells/ml) cultures of human peripheral blood neutrophils incubated with MSU crystals, PMA, Iono, or without stimulants for 3 h. Scale bar, 3 mm. B ) Scanning electron microscope micrographs from high-density (1 × 10 8 cells/ml) neutrophil cultures stimulated as indicated. Scale bars, 20 µm. C ) Representative multiphoton images of neutrophils stained with DNA dye SiR-DNA and membrane marker PKH67 and cultured in densities of 1 × 10 8 cells/ml with MSU crystals or without stimulant. Scale bars, 50 µm. D ) Assessment of MSU crystal–induced aggNETs with combination of multiphoton imaging and polarization microscopy. Scale bar, 50 µm. All experiments were performed independently 3 times.

    Article Snippet: Isolated human PMNs from healthy donors were cultured at densities ranging from 1 to 100 × 106 cells/ml (200,000 to 20 × 106 cells per well in flat-bottomed 96-well plates) with 20 pg/cell MSU crystals, 100 ng/ml PMA (MilliporeSigma), 5 µg/ml ionomycin (Iono; MilliporeSigma), 1 µM pyocyanin (MilliporeSigma), 15 µM nigericin (NIG; InvivoGen, San Diego, CA, USA), 2.5 µg/ml LPS from Klebsiella pneumoniae (MilliporeSigma), or without stimulus in HBSS medium containing 24 mM bicarbonate and 1 mM calcium at pH 7.4.

    Techniques: Incubation, Microscopy, Staining, Marker, Cell Culture, Imaging

    LTB 4 -induced BTK phosphorylation is required for inflammasome assembly. Macrophages were stimulated with 100 ng/mL LPS for 3h, followed by treatment with BTK inhibitor (Ibrutinib), PI3K inhibitor (wortmanin), Syk inhibitor (R406) and PKCδ (Rottlerin) for 20 min and 10nM LTB 4 for 10 min and 1uM nigericin treatment for 1h. A) IL-1β quantification by ELISA. B) Western blotting analysis of caspase-1 activation. The numbers represent mean densitometric analysis of the bands shown from 3 independent experiments. C) WT and BLT1 −/− mice were infected with MRSA for 24 h and skin lysates from WT or BLT1 −/− mice were subjected to immunoblotting, followed by detection of total and phosphorylated BTK (Tyr223). D) PLA of pBTK (Tyr223) and ASC in the infected skin of WT topically with vehicle control or LTB 4 . Data are the mean ± SEM of 5 mice from at least 2 individual experiments. *p

    Journal: bioRxiv

    Article Title: Leukotriene B4 licenses inflammasome activation to enhance skin host defense

    doi: 10.1101/2020.02.03.932129

    Figure Lengend Snippet: LTB 4 -induced BTK phosphorylation is required for inflammasome assembly. Macrophages were stimulated with 100 ng/mL LPS for 3h, followed by treatment with BTK inhibitor (Ibrutinib), PI3K inhibitor (wortmanin), Syk inhibitor (R406) and PKCδ (Rottlerin) for 20 min and 10nM LTB 4 for 10 min and 1uM nigericin treatment for 1h. A) IL-1β quantification by ELISA. B) Western blotting analysis of caspase-1 activation. The numbers represent mean densitometric analysis of the bands shown from 3 independent experiments. C) WT and BLT1 −/− mice were infected with MRSA for 24 h and skin lysates from WT or BLT1 −/− mice were subjected to immunoblotting, followed by detection of total and phosphorylated BTK (Tyr223). D) PLA of pBTK (Tyr223) and ASC in the infected skin of WT topically with vehicle control or LTB 4 . Data are the mean ± SEM of 5 mice from at least 2 individual experiments. *p

    Article Snippet: In vitro , phagocytes were challenged with LPS or GFP-MRSA at MOI 50:1 for 3h and pretreated with 10μM BLT1 antagonist U-75302 or 10nM LTB4 for 15 min, followed by inflammasome activation (MSU or nigericin), for 1 hour.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Activation Assay, Mouse Assay, Infection, Proximity Ligation Assay

    LTB 4 enhanced inflammasome assembly. Proximity-ligation assay (PLA) was used to detect associations between NLRP3 and ASC represented by red signal of A) Bone marrow neutrophils pretreated with 10nM LTB 4 for 5 minutes, 10μM U-75302 for 30 minutes and cultured with or without GFP-MRSA for 3h followed by 1μM nigericin for 1h and B) Peritoneal macrophages from WT or BLT1 −/− mice treated with 100ng/mL LPS for 3h followed by 10 μM MSU for 1h. C) Cleavage of IL-1β and caspase 1 evaluation by immunoblotting of macrophages treated with 100ng/mL LPS and 1μM nigericin in the presence of LTB 4 and BLT1 antagonist. D) Macrophages were stimulated with LTB 4 plus different inflammasome activators (Nigericin 1μM, flagellin 20μg/mL and Poly(dA:dT)/LyoVec 2μg/mL) and IL-1β was determined. Murine macrophages (E) and Human PMN (F) were treated with 100ng/mL LPS for 3h followed by 10nM LTB 4 and 1μM nigericin and active caspase-1 was measured on the supernatant. Quantification of the intensity of the red signal and western blotting bands was measured by ImageJ analysis. Data are mean ± SEM at least 10 cells from 3-6 mice *p

    Journal: bioRxiv

    Article Title: Leukotriene B4 licenses inflammasome activation to enhance skin host defense

    doi: 10.1101/2020.02.03.932129

    Figure Lengend Snippet: LTB 4 enhanced inflammasome assembly. Proximity-ligation assay (PLA) was used to detect associations between NLRP3 and ASC represented by red signal of A) Bone marrow neutrophils pretreated with 10nM LTB 4 for 5 minutes, 10μM U-75302 for 30 minutes and cultured with or without GFP-MRSA for 3h followed by 1μM nigericin for 1h and B) Peritoneal macrophages from WT or BLT1 −/− mice treated with 100ng/mL LPS for 3h followed by 10 μM MSU for 1h. C) Cleavage of IL-1β and caspase 1 evaluation by immunoblotting of macrophages treated with 100ng/mL LPS and 1μM nigericin in the presence of LTB 4 and BLT1 antagonist. D) Macrophages were stimulated with LTB 4 plus different inflammasome activators (Nigericin 1μM, flagellin 20μg/mL and Poly(dA:dT)/LyoVec 2μg/mL) and IL-1β was determined. Murine macrophages (E) and Human PMN (F) were treated with 100ng/mL LPS for 3h followed by 10nM LTB 4 and 1μM nigericin and active caspase-1 was measured on the supernatant. Quantification of the intensity of the red signal and western blotting bands was measured by ImageJ analysis. Data are mean ± SEM at least 10 cells from 3-6 mice *p

    Article Snippet: In vitro , phagocytes were challenged with LPS or GFP-MRSA at MOI 50:1 for 3h and pretreated with 10μM BLT1 antagonist U-75302 or 10nM LTB4 for 15 min, followed by inflammasome activation (MSU or nigericin), for 1 hour.

    Techniques: Proximity Ligation Assay, Cell Culture, Mouse Assay, Western Blot