mrs broth  (Millipore)


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  • 99
    Name:
    MRS Broth
    Description:

    Catalog Number:
    69966
    Price:
    None
    Applications:
    For the enrichment and isolation of all species of Lactobacilli from all types of material.
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    Structured Review

    Millipore mrs broth

    https://www.bioz.com/result/mrs broth/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mrs broth - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich). .. Monocolonization was confirmed by DNA extraction from the fecal pellet, PCR amplification of the full- length 16S rDNA region followed by Sanger sequencing.

    Transfection:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: Stable transfected TLR2ΔTIR Mode-K cells were established through serial plating in Puromycin-containing medium. .. B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80.

    Amplification:

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich). .. Monocolonization was confirmed by DNA extraction from the fecal pellet, PCR amplification of the full- length 16S rDNA region followed by Sanger sequencing.

    Plaque Formation Assay:

    Article Title: A Modified Zinc Acetate Gel, a Potential Nonantiretroviral Microbicide, Is Safe and Effective against Simian-Human Immunodeficiency Virus and Herpes Simplex Virus 2 Infection In Vivo
    Article Snippet: The aliquots of lactobacilli were frozen at −80°C in Lactobacilli MRS broth containing 15% glycerol (Sigma, St. Louis, MO). .. HSV-2 strain G (ATCC) was propagated in Vero cells (ATCC), and titers were determined using the plaque formation assay on Vero cells as previously described ( ).

    Polymerase Chain Reaction:

    Article Title: Interaction Between Familial Transmission and a Constitutively Active Immune System Shapes Gut Microbiota in Drosophila melanogaster
    Article Snippet: Culture-dependent identification of gut microbiota: A single fly was homogenized in LB (L3397, Sigma [Sigma Chemical], St. Louis, MO) or De Man, Rogosa, and Sharpe (MRS) broth (69966, Sigma) and serial dilutions were plated on both nutrient agar plates, LB (L3272, Sigma), and MRS (69964, Sigma). .. Colony PCR was performed using 16S rRNA eubacterial primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′).

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich). .. Monocolonization was confirmed by DNA extraction from the fecal pellet, PCR amplification of the full- length 16S rDNA region followed by Sanger sequencing.

    Isolation:

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: At 5 weeks of age SPF C57B1/6 mice were colonized with L. reuteri (LR) via daily oral gavage following the same conditions as for the monocolonization experiments, or with 0.2 ml of thawed 1 × 109 CFU/ml of L. johnsonii (LJ), isolated from the MLN of a TLR7.1 Tg mouse that showed signs of autoimmune disease. .. LJ was grown at 37°C in MRS broth (Sigma-Aldrich).

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: GF C57B1/6 mice were colonized at 6 weeks of age by a single oral gavage with 0.2 ml of thawed 1 × 109 CFU/ml of L. reuteri (LR), isolated from the spleen of a TLR7.1 Tg mouse that showed signs of autoimmune disease. .. The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich).

    Centrifugation:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80. .. Bacteria were harvested by centrifugation (3000 g , 15 min) at stationary growth phase, washed in phosphate-buffered saline (1× PBS pH 7·4) and diluted in DMEM (Invitrogen).

    Article Title: Echocardiographic Evaluation of Coronary Abnormalities and Cardiac Function in a Murine Model of Kawasaki Disease Using High-frequency Ultrasound
    Article Snippet: .. [ ] Briefly, L. casei (ATCC 11578) cultured in lactobacilli MRS broth (oxid, Britain) was harvested using centrifugation (9600 ×g , 30 min) during the log phase of growth and lysed with 4% sodium dodecyl sulfate (SDS, Sigma Systems, USA). .. Sequential incubations with 250 μg/ml RNase, DNase I and trypsin (Sigma Systems, USA) were performed to remove any adherent material from the cell walls.

    Article Title: Ιn Vitro Screening of γ-Aminobutyric Acid and Autoinducer-2 Signalling in Lactic Acid Bacteria Exhibiting Probiotic Potential Isolated from Natural Black Conservolea Olives
    Article Snippet: These cultures were used as the inoculum 1% (v /v ) to prepare the cultures used for the GABA determination in MRS broth supplemented with 10 mM L-glutamic acid (Sigma-Aldrich, Poole, United Kingdom) and were incubated under the same conditions. .. The population of each strain together with the pH values with the presence of L-glutamic acid after 48 h incubation were recorded, whereas the supernatant was collected from each culture with centrifugation (13,000 rpm for 10 min).

    Cell Culture:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: Paragraph title: Cell culture and bacterial stimulation ... B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80.

    Article Title: Echocardiographic Evaluation of Coronary Abnormalities and Cardiac Function in a Murine Model of Kawasaki Disease Using High-frequency Ultrasound
    Article Snippet: .. [ ] Briefly, L. casei (ATCC 11578) cultured in lactobacilli MRS broth (oxid, Britain) was harvested using centrifugation (9600 ×g , 30 min) during the log phase of growth and lysed with 4% sodium dodecyl sulfate (SDS, Sigma Systems, USA). .. Sequential incubations with 250 μg/ml RNase, DNase I and trypsin (Sigma Systems, USA) were performed to remove any adherent material from the cell walls.

    Article Title: Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods
    Article Snippet: .. Briefly, all strains were cultured in 4 mL MRS broth supplemented with 2% monosodium glutamate (MSG; Sigma-Aldrich, St. Louis, MO, USA) and anaerobically incubated at 30 °C for 2–3 days. .. After incubation, the culture broth was centrifuged at 8700 rpm at 4 °C for 5 min. One microliter of supernatant from each strain was spotted on a silica TLC plate (Aluminum Sheets Silica gel 60 F254, Merck).

    Mouse Assay:

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: At 5 weeks of age SPF C57B1/6 mice were colonized with L. reuteri (LR) via daily oral gavage following the same conditions as for the monocolonization experiments, or with 0.2 ml of thawed 1 × 109 CFU/ml of L. johnsonii (LJ), isolated from the MLN of a TLR7.1 Tg mouse that showed signs of autoimmune disease. .. LJ was grown at 37°C in MRS broth (Sigma-Aldrich).

    Article Title: Genetic and metabolic signals during acute enteric bacterial infection alter the microbiota and drive progression to chronic inflammatory disease
    Article Snippet: .. In some experiments when mice began exhibiting loose stools they were administered 100 µl of L. johnsonii and L. reuteri that had been grown overnight at 37°C in Lactobacilli MRS broth (EMD Chemicals, Gibbstown, NJ) and diluted to 1010 CFU/ by intragastric gavage. .. Terminal small intestine or proximal colon was removed, flushed with ice cold PBS and 1 mM DTT and were shaken at 37°C in HBSS with 2 mM EDTA (Sigma- Aldrich, St. Louis, MO) and 2 mM DTT (Sigma-Aldrich).

    Article Title: Echocardiographic Evaluation of Coronary Abnormalities and Cardiac Function in a Murine Model of Kawasaki Disease Using High-frequency Ultrasound
    Article Snippet: [ ] Briefly, L. casei (ATCC 11578) cultured in lactobacilli MRS broth (oxid, Britain) was harvested using centrifugation (9600 ×g , 30 min) during the log phase of growth and lysed with 4% sodium dodecyl sulfate (SDS, Sigma Systems, USA). .. Eighty mice were i.p. injected with 0.5 mg of LCWE in 0.5 ml of PBS to induce KD model.

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: GF C57B1/6 mice were colonized at 6 weeks of age by a single oral gavage with 0.2 ml of thawed 1 × 109 CFU/ml of L. reuteri (LR), isolated from the spleen of a TLR7.1 Tg mouse that showed signs of autoimmune disease. .. The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich).

    Activation Assay:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: We used Mode-K cells to selectively characterize Gram-positive bacteria-induced activation of epithelial cells. .. B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80.

    Incubation:

    Article Title: A Modified Zinc Acetate Gel, a Potential Nonantiretroviral Microbicide, Is Safe and Effective against Simian-Human Immunodeficiency Virus and Herpes Simplex Virus 2 Infection In Vivo
    Article Snippet: The aliquots of lactobacilli were frozen at −80°C in Lactobacilli MRS broth containing 15% glycerol (Sigma, St. Louis, MO). .. A single colony was picked and incubated in 5 ml of Sabouraud dextrose broth (SB; Sigma) overnight at 30°C with shaking at 150 rpm.

    Article Title: Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM ▿ NCFM ▿ †
    Article Snippet: Cells were resuspended in equal volumes of (i) MRS broth with 0.3% (wt/vol) porcine bile (Sigma), (ii) MRS broth with 2.5% (wt/vol) oxgall (Difco), (iii) MRS broth with 10% (wt/vol) NaCl, (iv) MRS broth with 15% (vol/vol) ethanol, and (v) acidified MRS broth at pH 3.5 (adjusted with lactic acid) and pH 3.0 (adjusted with HCl). .. Viable cell counts were determined after 2 h of incubation at 37°C by plating on MRS medium.

    Article Title: Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods
    Article Snippet: .. Briefly, all strains were cultured in 4 mL MRS broth supplemented with 2% monosodium glutamate (MSG; Sigma-Aldrich, St. Louis, MO, USA) and anaerobically incubated at 30 °C for 2–3 days. .. After incubation, the culture broth was centrifuged at 8700 rpm at 4 °C for 5 min. One microliter of supernatant from each strain was spotted on a silica TLC plate (Aluminum Sheets Silica gel 60 F254, Merck).

    Article Title: Common Distribution of gad Operon in Lactobacillus brevis and its GadA Contributes to Efficient GABA Synthesis toward Cytosolic Near-Neutral pH
    Article Snippet: Growth of Lb. brevis 145 in Lactobacilli MRS broth containing arginine, glutamate, agmatine, and tyrosine Cells of Lb. brevis 145 grown for 12 h (early stationary phase) in Lactobacilli MRS broth were inoculated into Lactobacilli MRS broth (pH 6.5) supplemented with or without the mixture of MSG, arginine, agmatine sulfate salt and tyrosine (1 g/L each; Sigma). .. The initial cell density after inoculation for acid challenge was 1.0 × 108 CFU/mL and the cells were incubated at 37°C for 36 h statically.

    Article Title: Ιn Vitro Screening of γ-Aminobutyric Acid and Autoinducer-2 Signalling in Lactic Acid Bacteria Exhibiting Probiotic Potential Isolated from Natural Black Conservolea Olives
    Article Snippet: .. These cultures were used as the inoculum 1% (v /v ) to prepare the cultures used for the GABA determination in MRS broth supplemented with 10 mM L-glutamic acid (Sigma-Aldrich, Poole, United Kingdom) and were incubated under the same conditions. .. The population of each strain together with the pH values with the presence of L-glutamic acid after 48 h incubation were recorded, whereas the supernatant was collected from each culture with centrifugation (13,000 rpm for 10 min).

    Thin Layer Chromatography:

    Article Title: Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods
    Article Snippet: Paragraph title: 2.5. Screening LAB Strains for GABA Production Using Thin Layer Chromatography (TLC) ... Briefly, all strains were cultured in 4 mL MRS broth supplemented with 2% monosodium glutamate (MSG; Sigma-Aldrich, St. Louis, MO, USA) and anaerobically incubated at 30 °C for 2–3 days.

    Activity Assay:

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host
    Article Snippet: BSH assay E. faecium strains were tested for hydrolase activity against glyco-conjugated bile acid as previously described [ ]. .. Overnight MRS broth cultures were streaked on MRS agar supplemented with 2 mM GDCA (Sigma; G3258).

    Infection:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80. .. Confluent epithelial cell monolayers were infected with B. lactis BB12 at a bacterium-to-epithelial cell ratio (multiplicity of infection (m.o.i.)) of 3–30 at various time points.

    Sequencing:

    Article Title: Interaction Between Familial Transmission and a Constitutively Active Immune System Shapes Gut Microbiota in Drosophila melanogaster
    Article Snippet: Culture-dependent identification of gut microbiota: A single fly was homogenized in LB (L3397, Sigma [Sigma Chemical], St. Louis, MO) or De Man, Rogosa, and Sharpe (MRS) broth (69966, Sigma) and serial dilutions were plated on both nutrient agar plates, LB (L3272, Sigma), and MRS (69964, Sigma). .. Culture-dependent identification of gut microbiota: A single fly was homogenized in LB (L3397, Sigma [Sigma Chemical], St. Louis, MO) or De Man, Rogosa, and Sharpe (MRS) broth (69966, Sigma) and serial dilutions were plated on both nutrient agar plates, LB (L3272, Sigma), and MRS (69964, Sigma).

    Article Title: A diet-sensitive commensal Lactobacillus strain mediates TLR7-dependent systemic autoimmunity
    Article Snippet: The LR strain was grown overnight at 37°C in MRS broth (Sigma-Aldrich). .. Monocolonization was confirmed by DNA extraction from the fecal pellet, PCR amplification of the full- length 16S rDNA region followed by Sanger sequencing.

    Sonication:

    Article Title: Echocardiographic Evaluation of Coronary Abnormalities and Cardiac Function in a Murine Model of Kawasaki Disease Using High-frequency Ultrasound
    Article Snippet: [ ] Briefly, L. casei (ATCC 11578) cultured in lactobacilli MRS broth (oxid, Britain) was harvested using centrifugation (9600 ×g , 30 min) during the log phase of growth and lysed with 4% sodium dodecyl sulfate (SDS, Sigma Systems, USA). .. After centrifugation, the pellet was sonicated (1 g packed wet weight in 3 ml phosphate buffered saline [PBS]) and further centrifuged with the resultant supernatant containing the LCWE.

    Injection:

    Article Title: Echocardiographic Evaluation of Coronary Abnormalities and Cardiac Function in a Murine Model of Kawasaki Disease Using High-frequency Ultrasound
    Article Snippet: [ ] Briefly, L. casei (ATCC 11578) cultured in lactobacilli MRS broth (oxid, Britain) was harvested using centrifugation (9600 ×g , 30 min) during the log phase of growth and lysed with 4% sodium dodecyl sulfate (SDS, Sigma Systems, USA). .. Eighty mice were i.p. injected with 0.5 mg of LCWE in 0.5 ml of PBS to induce KD model.

    Plasmid Preparation:

    Article Title: Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats
    Article Snippet: In addition, Mode-K cells were transfected with pZERO vector (InvivoGen, San Diego, CA) for murine TLR2ΔTIR lacking the cytoplasmic TIR domain. .. B. lactis BB12 was anaerobically grown at 37° in MRS broth (Sigma Aldrich, Taufkirchen, Germany) containing 1% Tween-80.

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  • 99
    Millipore lactobacilli mrs broth
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