mrna second strand synthesis module  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module
    Description:
    NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module 100 rxns
    Catalog Number:
    e6111l
    Price:
    1180
    Size:
    100 rxns
    Category:
    mRNA Template Preparation for PCR
    Buy from Supplier


    Structured Review

    New England Biolabs mrna second strand synthesis module
    NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module
    NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module 100 rxns
    https://www.bioz.com/result/mrna second strand synthesis module/product/New England Biolabs
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    mrna second strand synthesis module - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    RNA Sequencing Assay:

    Article Title: The hyper-activation of transcriptional enhancers in breast cancer
    Article Snippet: .. RNA-seq libraries were constructed by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111). .. Briefly, mRNA was extracted by poly-A selected with magnetic beads with poly-T and transformed into cDNA by first and second strand synthesis.

    Synthesized:

    Article Title: Classic oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer
    Article Snippet: .. Poly-adenylated RNA was enriched from 1ug of RNA for each sample with the NEBNext® PolyA mRNA Magnetic Isolation Module (NEB), incubated at 94°C for 15 min and double-strand cDNA was synthesized using SuperScript III reverse transcriptase (Thermo Fisher Scientific) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (NEB). .. Up to 10□ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB).

    Isolation:

    Article Title: The hyper-activation of transcriptional enhancers in breast cancer
    Article Snippet: .. RNA-seq libraries were constructed by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111). .. Briefly, mRNA was extracted by poly-A selected with magnetic beads with poly-T and transformed into cDNA by first and second strand synthesis.

    Article Title: Classic oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer
    Article Snippet: .. Poly-adenylated RNA was enriched from 1ug of RNA for each sample with the NEBNext® PolyA mRNA Magnetic Isolation Module (NEB), incubated at 94°C for 15 min and double-strand cDNA was synthesized using SuperScript III reverse transcriptase (Thermo Fisher Scientific) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (NEB). .. Up to 10□ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB).

    Ligation:

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: .. Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Generated:

    Article Title: Monitoring Viral Genetic Variation as a Tool To Improve Molecular Diagnostics for Mumps Virus
    Article Snippet: .. Second-strand cDNA was generated using the NEBNext Ultra II nondirectional RNA second-strand synthesis module (NEB, Ipswich, MA, USA). .. The resulting double-stranded cDNA was purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) and converted into Illumina sequencing libraries using the Nextera XT library preparation kit.

    Construct:

    Article Title: The hyper-activation of transcriptional enhancers in breast cancer
    Article Snippet: .. RNA-seq libraries were constructed by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111). .. Briefly, mRNA was extracted by poly-A selected with magnetic beads with poly-T and transformed into cDNA by first and second strand synthesis.

    Incubation:

    Article Title: Classic oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer
    Article Snippet: .. Poly-adenylated RNA was enriched from 1ug of RNA for each sample with the NEBNext® PolyA mRNA Magnetic Isolation Module (NEB), incubated at 94°C for 15 min and double-strand cDNA was synthesized using SuperScript III reverse transcriptase (Thermo Fisher Scientific) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (NEB). .. Up to 10□ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB).

    Polymerase Chain Reaction:

    Article Title: Library preparation for highly accurate population sequencing of RNA viruses
    Article Snippet: .. Random hexamers, 50 μM (Invitrogen, cat. no. N8080127) dNTP mix, 10 mM total (Bioline, cat. no. BIO-39053) SuperScript III reverse transcriptase, 200 U/μl (Invitrogen, cat. no. 18080-044) RNase H, 2 U/μl (Invitrogen, cat. no. 18021-071) NEBNext mRNA second strand synthesis module (New England Biolabs, cat. no. E6111S/L) NEBNext end repair module (New England Biolabs, cat. no. E6050S/L) NEBNext dA-tailing module (New England Biolabs, cat. no. E6053S/L) NEBNext quick ligation module (New England Biolabs, cat. no. E6056S/L) Phusion high-fidelity DNA polymerase (New England Biolabs, cat. no. M053S/L) Bromophenol Blue (Sigma-Aldrich, cat. no. B6131) Xylene cyanol (Affymetrix, cat. no. 23513) Perfect RNA Markers, 0.1–1 kb (EMD Millipore, cat. no. 69924) Low-molecular-weight DNA ladder, supplied with 6× gel loading dye (New England Biolabs, cat. no. N3233S/L) TruSeq indexed adapters and PCR primer cocktail (Illumina, cat. no. FC-121-4001) or equivalent oligonucleotides ▴ CRITICAL Indexed adapter oligonucleotides that are ordered separately should be annealed before use. .. Library quantification kit, Illumina/Universal (Kapa Biosystems, cat. no. KK4824) Sequencing kits, MiSeq reagent kit v2 (300 cycles; Illumina, cat. no. MS-102-2002) or 5× TruSeq rapid SBS kit—HS (50 cycle) and TruSeq rapid SR cluster kit—HS (Illumina, cat. nos.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs nebnext ultra ii directional rna second strand synthesis module
    Nebnext Ultra Ii Directional Rna Second Strand Synthesis Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii directional rna second strand synthesis module/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii directional rna second strand synthesis module - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs mrna second strand synthesis module
    Mrna Second Strand Synthesis Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna second strand synthesis module/product/New England Biolabs
    Average 99 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    mrna second strand synthesis module - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results