mpo staining  (Bioss)

Journal: bioRxiv

Article Title: Hepatocyte expression of fetal insulin receptor isoform contributes to the promotion of liver cancer through non-cell autonomous mechanisms

doi: 10.1101/2025.06.02.655477

Figure Lengend Snippet: Apc lox/lox mice were injected with recombinant AAV8 encoding saCas9 and sgRNA against Rosa26 (sgRosa/APC ΔHep , n=6) or Insr (sgInsr/APC ΔHep , n=5) locus. One month later, mice were injected with an adenovirus encoding the Cre recombinase (AdenoCre) and sacrificed 50 days later. A. Schematic illustration of the protocol. B. Liver-to-body weight ratio (n=5-6). C. Representative images of H&E staining showing microvacuolar steatosis, glycogenated nuclei, apoptotic bodies and inflammatory cells in sgInsr/APC Δhep livers. Scale bar: 50 μM. D. Representative images of myeloperoxidase (MPO) IHC staining from sgRosa/APC Δhep and sgInsr/APC Δhep livers ( left panel , scale bar: 120 μM); Quantification of MPO staining ( right panel ; n=5-6). E. Representative images of F4/80 IHC staining from sgRosa/APC Δhep and sgInsr/APC Δhep livers ( left panel , scale bar: 100 μM); Quantification of F4/80-positive macrophages ( right panel ; n=5-6). F. Representative images of proteome profiler cytokine/chemokine arrays performed on whole-cell lysates from sgRosa/APC Δhep and sgInsr/Apc Δhep livers (n=2). G. Expression of transcripts encoding chemokines/cytokines evaluated by RT-qPCR (n=5-6). H. Representative images of TUNEL assay from sgRosa/APC Δhep and sgInsr/APC Δhep livers ( left panel ); Quantification of TUNEL-positive cells ( right panel , n=5-6). Data are median ± IQR.

Article Snippet: For the assessment of liver steatosis, 10-μm liver frozen sections were fixed in 10% formalin for 10 min, rinsed in 60% isopropyl alcohol, and then incubated in Oil Red O solution (Sigma) for 15 min. IHC staining for myeloperoxidase (#22225-1-AP, Proteintech), glutamine synthetase (GS) (#610518, BD Transduction Laboratories), Ki67 (#MA5-14520, SP6, ThermoFisher Scientific), cleaved caspase-3 (#9661, D175) and F4/80 (#70076, D2S9R, Cell Signaling) was performed according to the provider instructions.

Techniques: Injection, Recombinant, Staining, Immunohistochemistry, Expressing, Quantitative RT-PCR, TUNEL Assay

Journal: Journal of advanced research

Article Title: Sedanolide alleviates DSS-induced colitis by modulating the intestinal FXR-SMPD3 pathway in mice.

doi: 10.1016/j.jare.2024.03.026

Figure Lengend Snippet: Fig. 6. Gut microbiota played a vital role in the protective effect of sedanolide against DSS-induced colitis in mice. (A) Experimental scheme. (B) Percent of initial weight of mice after DSS administration (left) and the weight of mice at the last day (right). (C) DAI score of mice after DSS administration. (D) The level of IL-6 and TNF-a in the serum. (E) Colon length of mice at the last day. (F) The level of FITC-dextran in serum. (G) Representative images of H&E staining of colon tissues (scale bar: 100 lm) and representative images of immunohistochemical staining for MPO, Occludin and ZO-1 in colon tissues (scale bar: 50 lm). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01.

Article Snippet: Immunohistochemical staining for myeloperoxidase (MPO) was performed using an anti-MPO antibody (Proteintech, Chicago, USA) according to the standard procedure.

Techniques: Staining, Immunohistochemical staining