mouse vegf r3 flt 4 antibody  (R&D Systems)

 
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    Name:
    Mouse VEGF R3 Flt 4 Antibody
    Description:
    The Mouse VEGF R3 Flt 4 Antibody from R D Systems is a goat polyclonal antibody to VEGF R3 Flt 4 This antibody reacts with mouse The Mouse VEGF R3 Flt 4 Antibody has been validated for the following applications Western Blot Flow Cytometry CyTOF ready
    Catalog Number:
    AF743
    Price:
    375
    Category:
    Primary Antibodies
    Applications:
    Western Blot, Flow Cytometry, CyTOF-ready
    Purity:
    Antigen Affinity-purified
    Conjugate:
    Unconjugated
    Immunogen:
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4, Tyr25-Asp770, Accession # P35917
    Size:
    100 ug
    Host:
    Goat
    Isotype:
    IgG
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    Structured Review

    R&D Systems mouse vegf r3 flt 4 antibody
    Mouse VEGF R3 Flt 4 Antibody
    The Mouse VEGF R3 Flt 4 Antibody from R D Systems is a goat polyclonal antibody to VEGF R3 Flt 4 This antibody reacts with mouse The Mouse VEGF R3 Flt 4 Antibody has been validated for the following applications Western Blot Flow Cytometry CyTOF ready
    https://www.bioz.com/result/mouse vegf r3 flt 4 antibody/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse vegf r3 flt 4 antibody - by Bioz Stars, 2021-03
    93/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Spatial regulation of VEGF receptor endocytosis in angiogenesis
    Article Snippet: Retinas were washed five times with 0.5% BSA and 0.15% Triton X-100 and incubated with Alexa-Fluor-coupled streptavidin (Invitrogen, 1:100) in blocking buffer for 2 hrs at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C: rabbit anti-VEGFR2 (Cell Signaling, 55B11, 1:100), rabbit anti-Dab2 (SantaCruz, H-110, 1:100), rabbit anti-PAR-3 , goat anti-VEGFR3 (R & D Systems, AF 743, 1:50), rabbit anti-collagen IV (Chemicon, AB756P, 1:200), rat VE-cadherin antibody (eBioscience, 555289, 1:100), and rabbit active aPKC (Abcam, ab59412, 1:100; Cell Signaling, 9378, lots 6 and 8, 1:100). .. After a washing step, retinas were incubated with the corresponding Alexa-Fluor-coupled secondary antibody (Invitrogen, 1:500) in blocking buffer for 2 hrs at room temperature.

    Incubation:

    Article Title: Identification of Novel Genes Associated with Renal Tertiary Lymphoid Organ Formation in Aging Mice
    Article Snippet: Immunofluorescence Four-micrometer thick frozen sections (C57BL6/J mouse kidney) were fixed in acetone (10 min., room temperature) and subsequently incubated in 0.03% H2 O2 (in PBS). .. Sections were pretreated with normal mouse serum (20 min., room temperature), and next incubated for 1 hr with primary antibody mixture consisting of hamster anti-mouse podoplanin (clone 811) with either goat anti-mouse VEGFR3 (R & D Systems) or rabbit anti-mouse LYVE-1 (kind gift from Prof. David Jackson, John Radcliffe University Hospital, Oxford, UK) diluted in PBS/1% BSA. .. Binding of primary antibodies was detected by incubating the sections for 30 min. with secondary polyclonal antibodies diluted in PBS +1% normal mouse serum: peroxidase-conjugated goat anti-Syrian hamster (Abcam) with FITC-conjugated rabbit anti-goat (DAKO) or FITC-conjugated goat anti-rabbit (DAKO).

    Article Title: VEGF-C induced angiogenesis preferentially occurs at a distance from lymphangiogenesis
    Article Snippet: .. The panels were blocked in 1% BSA-0.5% PBS-T 1 h. The mesentery was incubated overnight at 4°C, with biotinylated Griffonia simplicifolia isolectin B4 (IB4, Molecular Probes, Cambridge, UK) 10 μg/ml and mouse monoclonal antibodies to Ki-67 (Novocastra Lab, Newcastle upon Tyne, UK, NCL-L-Ki67-MM1), rabbit anti-mouse lymphatic vessel endothelial hyaluronic acid receptor (LYVE-1) (10 μg/μl, a kind gift from Dr David Jackson, University of Oxford), or goat-anti-mouse VEGFR-3 (1 μg/mL, R & D systems). .. Panels were washed as before, and TRITC labelled streptavidin (1 μg/mL, S-870, Molecular Probes) and Alexa Fluor 488, goat-anti-mouse IgG (2 μg/mL, Molecular Probes) were used as secondary detection antibodies to Lectin and Ki67, respectively.

    Immunostaining:

    Article Title: Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Article Snippet: .. Primary antibodies used for immunostaining were as follows: rabbit anti-PROX1 (AngioBio), goat anti-human PROX1 (R & D Systems), sheep anti-mouse FOXC2 (R & D Systems), goat anti-mouse VEGFR3 (R & D Systems), goat anti-mouse LYVE1 (R & D Systems), rat anti-mouse CD31 (BD Pharmingen), goat anti-mouse GATA2 (R & D Systems), rabbit anti-total β-catenin and rabbit anti-active β-catenin antibodies (both from Cell Signaling Technologies), goat anti-mouse NRP2 (R & D Systems), chicken anti-GFP (Abcam), rat anti-mouse Endomucin (eBioscience), rabbit anti-CX37 (Invitrogen), and Cy3-conjugated monoclonal anti-α-SMA (Sigma-Aldrich). .. The following secondary antibodies were used: Cy3-conjugated donkey anti-rabbit, Cy3-conjugated donkey anti-sheep, and Cy5-conjugated donkey anti-rat (all purchased from Jackson ImmunoResearch Laboratories).

    Immunoprecipitation:

    Article Title: Transgenic Induction of Vascular Endothelial Growth Factor-C Is Strongly Angiogenic in Mouse Embryos but Leads to Persistent Lymphatic Hyperplasia in Adult Tissues
    Article Snippet: .. VEGFR-3 was immunoprecipitated using goat polyclonal anti-mouse VEGFR-3 antibody (1 μg, AF743; R & D Systems). .. The immunocomplexes were captured using protein-G Sepharose (GE Health Care Bio-Sciences, Uppsala, Sweden), separated by 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a nitrocellulose membrane, and detected using mouse monoclonal anti-phosphotyrosine (67 ng/ml, 4G10; Upstate Biotechnology, Lake Placid, NY) and goat polyclonal anti-mouse VEGFR-3 0.1 μg/ml, (AF743; R & D Systems) antibodies, biotinylated anti-goat (0.64 μg/ml) or anti-mouse (0.3 μg/ml) secondary antibodies (DAKO, Glostrup, Denmark), and streptavidin-biotinylated horseradish peroxidase complex in 1:5000 dilution (GE Health Care, Little Chalfont, UK) followed by enhanced chemiluminescence detection with the SuperSignal West Femto maximum sensitivity substrate (Pierce, Rockford, IL).

    Staining:

    Article Title: Ricinus communis Agglutinin I Leads to Rapid Down-Regulation of VEGFR-2 and Endothelial Cell Apoptosis in Tumor Blood Vessels
    Article Snippet: Specimens were rinsed with PBS, infiltrated with 30% sucrose in PBS overnight at 4°C, embedded in optimal cutting temperature compound (Sakura Finetek), and frozen at −20°C. .. Endothelial cells of tumor vessels were stained by immunohistochemistry using our previously described methods , , with one or more antibodies: rat anti-mouse CD31 (PECAM-1, clone MEC 13.3, 1:500; BD Pharmingen), hamster anti-mouse CD31 (clone 2H8, 1:500; Chemicon), rabbit anti-mouse VEGFR-2 (TO14, 1:2000; gift from Rolf Brekken, University of Texas Southwestern Medical Center), goat anti-mouse VEGFR-3 (1:1000; R & D Systems), or rat anti-mouse CD105 (endoglin, clone MJ7/18, 1:500; BD Pharmingen). .. VEGF was stained with goat anti-mouse VEGF-A (anti-VEGF, 1:50; R & D systems).

    Immunohistochemistry:

    Article Title: Ricinus communis Agglutinin I Leads to Rapid Down-Regulation of VEGFR-2 and Endothelial Cell Apoptosis in Tumor Blood Vessels
    Article Snippet: Specimens were rinsed with PBS, infiltrated with 30% sucrose in PBS overnight at 4°C, embedded in optimal cutting temperature compound (Sakura Finetek), and frozen at −20°C. .. Endothelial cells of tumor vessels were stained by immunohistochemistry using our previously described methods , , with one or more antibodies: rat anti-mouse CD31 (PECAM-1, clone MEC 13.3, 1:500; BD Pharmingen), hamster anti-mouse CD31 (clone 2H8, 1:500; Chemicon), rabbit anti-mouse VEGFR-2 (TO14, 1:2000; gift from Rolf Brekken, University of Texas Southwestern Medical Center), goat anti-mouse VEGFR-3 (1:1000; R & D Systems), or rat anti-mouse CD105 (endoglin, clone MJ7/18, 1:500; BD Pharmingen). .. VEGF was stained with goat anti-mouse VEGF-A (anti-VEGF, 1:50; R & D systems).

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  • 90
    R&D Systems vegfr3
    Characterization of CCSP-rtTA × tet-O–VEGF-C mice after doxycycline treatment for 2 weeks. ( A ) VEGF-C mRNA expression in the lung (normalized to RPLP0). The average expression level in WT × tet-O–VEGF-C mice was set to 1 ( n = 6). ( B ) VEGF-C protein levels in the lung detected by enzyme-linked immunosorbent assay (ELISA) ( n = 6). ( C ) Quantification of the <t>VEGFR3</t> + LV area ( n = 4). ( D ) Representative images of VEGFR3 staining in the lung. Scale bars, 1 mm. ( E ) Representative high-magnification images of different regions in the lung stained for VEGFR3. Scale bars, 200 μm. PA, pulmonary artery; PV, pulmonary vein. ( F ) Representative images of MECA-32 staining in the lung. Scale bars, 50 μm. ( G ) Quantification of MECA-32 + blood vessel area in the lung ( n = 5).
    Vegfr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegfr3/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegfr3 - by Bioz Stars, 2021-03
    90/100 stars
      Buy from Supplier

    92
    R&D Systems mouse anti vegf a monoclonal antibody
    Immunohistochemical staining for <t>VEGF-A</t> xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.
    Mouse Anti Vegf A Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vegf a monoclonal antibody/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti vegf a monoclonal antibody - by Bioz Stars, 2021-03
    92/100 stars
      Buy from Supplier

    90
    R&D Systems pe conjugated mouse anti human vegfr 3
    NFATc1 is coexpressed with Prox-1 and <t>VEGFR-3</t> on developing and mature lymphatic endothelial cells
    Pe Conjugated Mouse Anti Human Vegfr 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated mouse anti human vegfr 3/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated mouse anti human vegfr 3 - by Bioz Stars, 2021-03
    90/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of CCSP-rtTA × tet-O–VEGF-C mice after doxycycline treatment for 2 weeks. ( A ) VEGF-C mRNA expression in the lung (normalized to RPLP0). The average expression level in WT × tet-O–VEGF-C mice was set to 1 ( n = 6). ( B ) VEGF-C protein levels in the lung detected by enzyme-linked immunosorbent assay (ELISA) ( n = 6). ( C ) Quantification of the VEGFR3 + LV area ( n = 4). ( D ) Representative images of VEGFR3 staining in the lung. Scale bars, 1 mm. ( E ) Representative high-magnification images of different regions in the lung stained for VEGFR3. Scale bars, 200 μm. PA, pulmonary artery; PV, pulmonary vein. ( F ) Representative images of MECA-32 staining in the lung. Scale bars, 50 μm. ( G ) Quantification of MECA-32 + blood vessel area in the lung ( n = 5).

    Journal: Science Advances

    Article Title: Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread

    doi: 10.1126/sciadv.aat4758

    Figure Lengend Snippet: Characterization of CCSP-rtTA × tet-O–VEGF-C mice after doxycycline treatment for 2 weeks. ( A ) VEGF-C mRNA expression in the lung (normalized to RPLP0). The average expression level in WT × tet-O–VEGF-C mice was set to 1 ( n = 6). ( B ) VEGF-C protein levels in the lung detected by enzyme-linked immunosorbent assay (ELISA) ( n = 6). ( C ) Quantification of the VEGFR3 + LV area ( n = 4). ( D ) Representative images of VEGFR3 staining in the lung. Scale bars, 1 mm. ( E ) Representative high-magnification images of different regions in the lung stained for VEGFR3. Scale bars, 200 μm. PA, pulmonary artery; PV, pulmonary vein. ( F ) Representative images of MECA-32 staining in the lung. Scale bars, 50 μm. ( G ) Quantification of MECA-32 + blood vessel area in the lung ( n = 5).

    Article Snippet: The antibodies used were Thy1.2 (rat, eBioscience), cytokeratin (wide spectrum, rabbit, Dako), LYVE-1 (rabbit, AngioBio), αSMA-Cy3 (mouse, Sigma-Aldrich), VEGFR3 (goat, R & D Systems), MECA-32 (rat, BD), RFP (rabbit, Rockland), podoplanin (goat, R & D Systems), and CD31 (rat, BD Pharmingen).

    Techniques: Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Staining

    Immunohistochemical staining for VEGF-A xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.

    Journal: American Journal of Cancer Research

    Article Title: Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios

    doi:

    Figure Lengend Snippet: Immunohistochemical staining for VEGF-A xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.

    Article Snippet: An Immunoassay 96 well plate (Thermo Life Sciences) was coated with a mouse anti-VEGF-A165 b monoclonal antibody (Clone 56/1, MAB3045, R & D Systems) at a concentration of 10 µg/ml, covered with parafilm and protected from light, then left shaking at room temperature (RT) overnight (~16 hours). pan VEGF-A capture was mouse anti-VEGF-A monoclonal antibody at 2 µg/ml (MAB293, R & D Systems).

    Techniques: Immunohistochemistry, Staining

    Predictive staining intensity ratio correlates with plasma VEGF-A xxx b level. A. Correlation of ratio of score of VEGF-A xxx b: panVEGF with plasma VEGF-A xxx b level. r=0.594, P

    Journal: American Journal of Cancer Research

    Article Title: Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios

    doi:

    Figure Lengend Snippet: Predictive staining intensity ratio correlates with plasma VEGF-A xxx b level. A. Correlation of ratio of score of VEGF-A xxx b: panVEGF with plasma VEGF-A xxx b level. r=0.594, P

    Article Snippet: An Immunoassay 96 well plate (Thermo Life Sciences) was coated with a mouse anti-VEGF-A165 b monoclonal antibody (Clone 56/1, MAB3045, R & D Systems) at a concentration of 10 µg/ml, covered with parafilm and protected from light, then left shaking at room temperature (RT) overnight (~16 hours). pan VEGF-A capture was mouse anti-VEGF-A monoclonal antibody at 2 µg/ml (MAB293, R & D Systems).

    Techniques: Staining

    VEGF-A xxx b levels measured in plasma. A. Frequency distribution of sample values. B. VEGF-A xxx b levels before and after surgery. BDL= below detection limit. C. Correlation of VEGF-A xxx b levels before and after treatment. R=0.815, P

    Journal: American Journal of Cancer Research

    Article Title: Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios

    doi:

    Figure Lengend Snippet: VEGF-A xxx b levels measured in plasma. A. Frequency distribution of sample values. B. VEGF-A xxx b levels before and after surgery. BDL= below detection limit. C. Correlation of VEGF-A xxx b levels before and after treatment. R=0.815, P

    Article Snippet: An Immunoassay 96 well plate (Thermo Life Sciences) was coated with a mouse anti-VEGF-A165 b monoclonal antibody (Clone 56/1, MAB3045, R & D Systems) at a concentration of 10 µg/ml, covered with parafilm and protected from light, then left shaking at room temperature (RT) overnight (~16 hours). pan VEGF-A capture was mouse anti-VEGF-A monoclonal antibody at 2 µg/ml (MAB293, R & D Systems).

    Techniques:

    Lymphatic vessels are largely restricted to the myometrium in mouse uterus . (A) Representative photomicrograph of podoplanin (brown) and CD31 (blue) dual-immunostaining in mouse uterus highlighting the lymphatic and blood vessels, respectively. An area of a transverse section is shown. (B) Higher magnification of tissue within boxed area in A. Note the pale but distinct non-lymphatic podoplanin immunoreactivity in the connective tissue around the myometrial arteriole (white arrow). (C) Lyve-1 immunostaining is also useful for identifying uterine lymphatic vessels, as seen in this photomicrograph from a section serial to that in B. (D) VEGFR-3 immunostaining in a section serial to that in C (Inset: isotype-matched control for VEGFR-3). VEGFR-3 immunoreactivity is not useful as a marker of uterine lymphatics as varying levels of immunostaining are also observed in other uterine cellular components. (E, F) Representative photomicrographs of human VEGF-D (brown)/Lyve-1 (blue) and (G, H) VEGFR-3 immunostaining in NOD SCID mouse uterus inoculated with VEGF-D expressing 293EBNA cells. Note: F and H are higher magnification images of tissue within boxed area in E and G, respectively. Black arrows: lymphatic vessels. Dotted line delineates myometrium and endometrium. en: endometrium, m: myometrium, t: tumor.

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Vascular endothelial growth factor-D over-expressing tumor cells induce differential effects on uterine vasculature in a mouse model of endometrial cancer

    doi: 10.1186/1477-7827-8-84

    Figure Lengend Snippet: Lymphatic vessels are largely restricted to the myometrium in mouse uterus . (A) Representative photomicrograph of podoplanin (brown) and CD31 (blue) dual-immunostaining in mouse uterus highlighting the lymphatic and blood vessels, respectively. An area of a transverse section is shown. (B) Higher magnification of tissue within boxed area in A. Note the pale but distinct non-lymphatic podoplanin immunoreactivity in the connective tissue around the myometrial arteriole (white arrow). (C) Lyve-1 immunostaining is also useful for identifying uterine lymphatic vessels, as seen in this photomicrograph from a section serial to that in B. (D) VEGFR-3 immunostaining in a section serial to that in C (Inset: isotype-matched control for VEGFR-3). VEGFR-3 immunoreactivity is not useful as a marker of uterine lymphatics as varying levels of immunostaining are also observed in other uterine cellular components. (E, F) Representative photomicrographs of human VEGF-D (brown)/Lyve-1 (blue) and (G, H) VEGFR-3 immunostaining in NOD SCID mouse uterus inoculated with VEGF-D expressing 293EBNA cells. Note: F and H are higher magnification images of tissue within boxed area in E and G, respectively. Black arrows: lymphatic vessels. Dotted line delineates myometrium and endometrium. en: endometrium, m: myometrium, t: tumor.

    Article Snippet: Immunohistochemistry Antibodies against Lyve-1 (polyclonal goat anti-human Lyve-1, 1 μg/ml; #AF2089, R & D, Minneapolis, MN, USA), podoplanin (goat anti-mouse podoplanin, 1 μg/ml, #AF3244, R & D) and VEGFR-3 (monoclonal rat anti VEGFR-3, 2 μg/ml; clone AFL4, now commercially available from R & D Systems) were used to identify lymphatic vessels.

    Techniques: Immunostaining, Marker, Expressing

    NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells

    Journal: Mechanisms of development

    Article Title: NFATc1 regulates lymphatic endothelial development

    doi: 10.1016/j.mod.2009.02.003

    Figure Lengend Snippet: NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells

    Article Snippet: For flow cytometry, cells were lifted with 0.02% EDTA (Gibco), washed with phosphate-buffered saline (PBS), blocked and incubated with PE-conjugated mouse anti-human VEGFR-3 (R & D Systems), mouse anti-human podoplanin (Fitzgerald Industries) or isotype controls for 1 hour.

    Techniques: