mouse vegf elisa kit  (R&D Systems)

 
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    Name:
    Recombinant Mouse VEGF D Protein
    Description:
    The Recombinant Mouse VEGF D Protein from R D Systems is derived from Sf 21 baculovirus The Recombinant Mouse VEGF D Protein has been validated for the following applications Bioactivity Binding Activity
    Catalog Number:
    469-VD-025
    Price:
    329
    Category:
    Proteins and Enzymes
    Source:
    Sf 21 (baculovirus)-derived Recombinant Mouse VEGF-D Protein
    Applications:
    Bioactivity, Binding Activity
    Purity:
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
    Conjugate:
    Unconjugated
    Size:
    25 ug
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    Structured Review

    R&D Systems mouse vegf elisa kit
    NGF increased the expression of <t>VEGF</t> in Müller cells. Müller cells were treated with 100 ng/ml of nerve growth factor (NGF) compared with an equal amount of bovine serum albumin (BSA) control. After 12 or 24 h, vascular endothelial growth factor (VEGF) protein expression in the supernatants was significantly increased in the NGF-treated group according to the enzyme-linked immunosorbent assay <t>(ELISA).</t> Importantly, the VEGF increase after 24 h was significantly higher than that after the 12 h treatments. There was no statistically significant difference between 12 and 24 h in the BSA treatment group with a p value equal to 0.0644. n=6, *p
    The Recombinant Mouse VEGF D Protein from R D Systems is derived from Sf 21 baculovirus The Recombinant Mouse VEGF D Protein has been validated for the following applications Bioactivity Binding Activity
    https://www.bioz.com/result/mouse vegf elisa kit/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse vegf elisa kit - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "NGF increases VEGF expression and promotes cell proliferation via ERK1/2 and AKT signaling in Müller cells"

    Article Title: NGF increases VEGF expression and promotes cell proliferation via ERK1/2 and AKT signaling in Müller cells

    Journal: Molecular Vision

    doi:

    NGF increased the expression of VEGF in Müller cells. Müller cells were treated with 100 ng/ml of nerve growth factor (NGF) compared with an equal amount of bovine serum albumin (BSA) control. After 12 or 24 h, vascular endothelial growth factor (VEGF) protein expression in the supernatants was significantly increased in the NGF-treated group according to the enzyme-linked immunosorbent assay (ELISA). Importantly, the VEGF increase after 24 h was significantly higher than that after the 12 h treatments. There was no statistically significant difference between 12 and 24 h in the BSA treatment group with a p value equal to 0.0644. n=6, *p
    Figure Legend Snippet: NGF increased the expression of VEGF in Müller cells. Müller cells were treated with 100 ng/ml of nerve growth factor (NGF) compared with an equal amount of bovine serum albumin (BSA) control. After 12 or 24 h, vascular endothelial growth factor (VEGF) protein expression in the supernatants was significantly increased in the NGF-treated group according to the enzyme-linked immunosorbent assay (ELISA). Importantly, the VEGF increase after 24 h was significantly higher than that after the 12 h treatments. There was no statistically significant difference between 12 and 24 h in the BSA treatment group with a p value equal to 0.0644. n=6, *p

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    NGF increases VEGF expression via the TrkA receptor and is mediated by the activation of ERK1/2 and AKT signaling. Müller cells were treated with 100 ng/ml of nerve growth factor (NGF) with or without 20 nM K252a (TrkA inhibitor), 10 µM U0126 (extracellular signal-regulated kinases 1/2 (ERK1/2) pathway inhibitor), or 10 µM LY294002 (phosphatidylinositol 3-kinase (PI3K)/AKT inhibitor) for 12 or 24 h. The vascular endothelial growth factor (VEGF) protein level in the supernatants was detected with enzyme-linked immunosorbent assay (ELISA). The TrkA, ERK1/2, and PI3K/AKT inhibitors decreased the ability of NGF to promote VEGF expression to some extent. n=6, * p
    Figure Legend Snippet: NGF increases VEGF expression via the TrkA receptor and is mediated by the activation of ERK1/2 and AKT signaling. Müller cells were treated with 100 ng/ml of nerve growth factor (NGF) with or without 20 nM K252a (TrkA inhibitor), 10 µM U0126 (extracellular signal-regulated kinases 1/2 (ERK1/2) pathway inhibitor), or 10 µM LY294002 (phosphatidylinositol 3-kinase (PI3K)/AKT inhibitor) for 12 or 24 h. The vascular endothelial growth factor (VEGF) protein level in the supernatants was detected with enzyme-linked immunosorbent assay (ELISA). The TrkA, ERK1/2, and PI3K/AKT inhibitors decreased the ability of NGF to promote VEGF expression to some extent. n=6, * p

    Techniques Used: Expressing, Activation Assay, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice"

    Article Title: Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0152179

    Growth factor production of cardiac stem cells (CSCs). The supernatants of twice-passaged CSCs were collected to measure the concentration of vascular endothelial growth factor (VEGF) (A) and insulin-like growth factor-1 (IGF-1) (B) by ELISA. Values shown are the mean ± SEM from 3 independent experiments.
    Figure Legend Snippet: Growth factor production of cardiac stem cells (CSCs). The supernatants of twice-passaged CSCs were collected to measure the concentration of vascular endothelial growth factor (VEGF) (A) and insulin-like growth factor-1 (IGF-1) (B) by ELISA. Values shown are the mean ± SEM from 3 independent experiments.

    Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay

    3) Product Images from "Poly(oligo--arginine) With Internal Disulfide Linkages as a Cytoplasm-sensitive Carrier for siRNA Delivery"

    Article Title: Poly(oligo--arginine) With Internal Disulfide Linkages as a Cytoplasm-sensitive Carrier for siRNA Delivery

    Journal: Molecular Therapy

    doi: 10.1038/mt.2010.242

    In vitro VEGF silencing . ( a ) The polyplexes prepared with 100 pmol siVEGF at a ratio of 5 were transfected into SCC cells for 48 hours. VEGF secreted from cells was quantified using an ELISA kit (* P
    Figure Legend Snippet: In vitro VEGF silencing . ( a ) The polyplexes prepared with 100 pmol siVEGF at a ratio of 5 were transfected into SCC cells for 48 hours. VEGF secreted from cells was quantified using an ELISA kit (* P

    Techniques Used: In Vitro, Transfection, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Suppression and Regression of Choroidal Neovascularization in Mice by a Novel CCR2 Antagonist, INCB3344"

    Article Title: Suppression and Regression of Choroidal Neovascularization in Mice by a Novel CCR2 Antagonist, INCB3344

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028933

    VEGF expression. ( A ) Immunohistochemistry of macrophages (green) and VEGF (red) in cryosections on Day 3. Significantly higher levels of VEGF were expressed in macrophages at the photocoagulated sites. VEGF localized mainly in infiltrating macrophages at the laser injury sites. INCB3344 apparently decreased VEGF immunoreactivity compared to vehicle treatment. The negative control omitting the primary antibody (second antibody only) had detectable auto-fluorescence in RPE. Scale bar = 100 µm. ( B ) VEGF protein levels in the choroid-RPE were quantitatively measured by ELISA. VEGF levels on Day 3 were significantly suppressed by INCB3344 treatment. ( n = 8, * P = 0.012). ( C ) The expression of VEGF mRNA derived from macrophages isolated from choroid-RPE complexes was detected by real-time PCR on Day 3 after photocoagulation. The increased VEGF mRNA expression in infiltrating macrophages was significantly suppressed by INCB3344 treatment (**,*** P
    Figure Legend Snippet: VEGF expression. ( A ) Immunohistochemistry of macrophages (green) and VEGF (red) in cryosections on Day 3. Significantly higher levels of VEGF were expressed in macrophages at the photocoagulated sites. VEGF localized mainly in infiltrating macrophages at the laser injury sites. INCB3344 apparently decreased VEGF immunoreactivity compared to vehicle treatment. The negative control omitting the primary antibody (second antibody only) had detectable auto-fluorescence in RPE. Scale bar = 100 µm. ( B ) VEGF protein levels in the choroid-RPE were quantitatively measured by ELISA. VEGF levels on Day 3 were significantly suppressed by INCB3344 treatment. ( n = 8, * P = 0.012). ( C ) The expression of VEGF mRNA derived from macrophages isolated from choroid-RPE complexes was detected by real-time PCR on Day 3 after photocoagulation. The increased VEGF mRNA expression in infiltrating macrophages was significantly suppressed by INCB3344 treatment (**,*** P

    Techniques Used: Expressing, Immunohistochemistry, Negative Control, Fluorescence, Enzyme-linked Immunosorbent Assay, Derivative Assay, Isolation, Real-time Polymerase Chain Reaction

    5) Product Images from "β2–Adrenergic Receptor Antagonism Attenuates CNV Through Inhibition of VEGF and IL-6 Expression"

    Article Title: β2–Adrenergic Receptor Antagonism Attenuates CNV Through Inhibition of VEGF and IL-6 Expression

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.16-20204

    β2–Adrenergic receptor inhibition suppresses IL-6 protein expression in laser-induced CNV. Mice were given a single intravitreal injection of saline (veh) or β2-AR antagonist (ICI-118,551, 0.03 μg per eye) on the same day as laser treatment. Total eye lysates were prepared, and eyes were combined for each mouse. ( A ) Vascular endothelial growth factor expression was measured by ELISA on day 5 ( N = 3–4, P > 0.05). ( B ) Interleukin-6 protein expression was measured by ELISA on day 3 ( N = 3, *** P
    Figure Legend Snippet: β2–Adrenergic receptor inhibition suppresses IL-6 protein expression in laser-induced CNV. Mice were given a single intravitreal injection of saline (veh) or β2-AR antagonist (ICI-118,551, 0.03 μg per eye) on the same day as laser treatment. Total eye lysates were prepared, and eyes were combined for each mouse. ( A ) Vascular endothelial growth factor expression was measured by ELISA on day 5 ( N = 3–4, P > 0.05). ( B ) Interleukin-6 protein expression was measured by ELISA on day 3 ( N = 3, *** P

    Techniques Used: Inhibition, Expressing, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    6) Product Images from "Neuroprotective response after photodynamic therapy: Role of vascular endothelial growth factor"

    Article Title: Neuroprotective response after photodynamic therapy: Role of vascular endothelial growth factor

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-8-176

    VEGF expression in the RPE-choroid complex . (A) vegf mRNA expression in the RPE-choroid complex analyzed by real-time (RT)-PCR. vegf mRNA did not increase after PDT. (B) VEGF protein expression analyzed by ELISA. VEGF protein decreased transiently 1 day after PDT. * p
    Figure Legend Snippet: VEGF expression in the RPE-choroid complex . (A) vegf mRNA expression in the RPE-choroid complex analyzed by real-time (RT)-PCR. vegf mRNA did not increase after PDT. (B) VEGF protein expression analyzed by ELISA. VEGF protein decreased transiently 1 day after PDT. * p

    Techniques Used: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    VEGF induction in retina after PDT . (A) vegf mRNA expression in neural retina analyzed by real-time (RT)-PCR. vegf mRNA was upregulated transiently 1.5 hours after PDT. (B) VEGF protein expression analyzed by ELISA. VEGF protein increased transiently 3 hours after PDT. * p
    Figure Legend Snippet: VEGF induction in retina after PDT . (A) vegf mRNA expression in neural retina analyzed by real-time (RT)-PCR. vegf mRNA was upregulated transiently 1.5 hours after PDT. (B) VEGF protein expression analyzed by ELISA. VEGF protein increased transiently 3 hours after PDT. * p

    Techniques Used: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    7) Product Images from "AAV2 Delivery of Flt23k Intraceptors Inhibits Murine Choroidal Neovascularization"

    Article Title: AAV2 Delivery of Flt23k Intraceptors Inhibits Murine Choroidal Neovascularization

    Journal: Molecular Therapy

    doi: 10.1038/mt.2014.199

    Flt23k reduces vascular endothelial growth factor (VEGF)-A protein levels in HeLa cells . ( a ) Western blot analysis reveals Flt23k over expression and VEGF suppression in pGENIE.Flt23k transfected cells compared to control. ( b ) Enzyme-linked immunosorbent assay (ELISA) analysis demonstrates reduced Hela culture medium VEGF levels in pGENIE.Flt23k transfected cells compared to control in both normoxic and hypoxic conditions.
    Figure Legend Snippet: Flt23k reduces vascular endothelial growth factor (VEGF)-A protein levels in HeLa cells . ( a ) Western blot analysis reveals Flt23k over expression and VEGF suppression in pGENIE.Flt23k transfected cells compared to control. ( b ) Enzyme-linked immunosorbent assay (ELISA) analysis demonstrates reduced Hela culture medium VEGF levels in pGENIE.Flt23k transfected cells compared to control in both normoxic and hypoxic conditions.

    Techniques Used: Western Blot, Over Expression, Transfection, Enzyme-linked Immunosorbent Assay

    8) Product Images from "Poly(oligo--arginine) With Internal Disulfide Linkages as a Cytoplasm-sensitive Carrier for siRNA Delivery"

    Article Title: Poly(oligo--arginine) With Internal Disulfide Linkages as a Cytoplasm-sensitive Carrier for siRNA Delivery

    Journal: Molecular Therapy

    doi: 10.1038/mt.2010.242

    In vitro VEGF silencing . ( a ) The polyplexes prepared with 100 pmol siVEGF at a ratio of 5 were transfected into SCC cells for 48 hours. VEGF secreted from cells was quantified using an ELISA kit (* P
    Figure Legend Snippet: In vitro VEGF silencing . ( a ) The polyplexes prepared with 100 pmol siVEGF at a ratio of 5 were transfected into SCC cells for 48 hours. VEGF secreted from cells was quantified using an ELISA kit (* P

    Techniques Used: In Vitro, Transfection, Enzyme-linked Immunosorbent Assay

    9) Product Images from "Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells"

    Article Title: Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Müller cells

    Journal: Diabetologia

    doi: 10.1007/s00125-011-2081-0

    Attenuated VEGF over-production in retinas of Hif-1α KO mice. a, d Immunohisto-chemical staining of VEGF (red) in wild-type (WT) and the Hif-1α KO mice under normoxia or ( g, j ) with OIR at age P17. b, e, h, k GS staining (green) and ( c, f, i, l ) merged VEGF and GS staining with DAPI staining (blue) in mice as above ( a, d, g, j ). Scale bar 50 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. m Retinal VEGF was determined by western blot analysis using 20 μg retinal protein from each mouse. n Densitometry of the western blot showed that VEGF was significantly lower in the retina of Hif-1α KO mice (black bars) with OIR than in WT mice (white bars) with OIR. o Retinal VEGF levels were also measured with ELISA, showing that the overproduction of VEGF was attenuated in Hif-1α KO mice under hypoxic conditions. Values ( n, o ) are mean±SEM; n =6; * p
    Figure Legend Snippet: Attenuated VEGF over-production in retinas of Hif-1α KO mice. a, d Immunohisto-chemical staining of VEGF (red) in wild-type (WT) and the Hif-1α KO mice under normoxia or ( g, j ) with OIR at age P17. b, e, h, k GS staining (green) and ( c, f, i, l ) merged VEGF and GS staining with DAPI staining (blue) in mice as above ( a, d, g, j ). Scale bar 50 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. m Retinal VEGF was determined by western blot analysis using 20 μg retinal protein from each mouse. n Densitometry of the western blot showed that VEGF was significantly lower in the retina of Hif-1α KO mice (black bars) with OIR than in WT mice (white bars) with OIR. o Retinal VEGF levels were also measured with ELISA, showing that the overproduction of VEGF was attenuated in Hif-1α KO mice under hypoxic conditions. Values ( n, o ) are mean±SEM; n =6; * p

    Techniques Used: Mouse Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay

    10) Product Images from "Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice"

    Article Title: Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice

    Journal: Scientific Reports

    doi: 10.1038/srep40959

    Growth factors production of cardiac explant-derived cells (CDCs). CDCs were expanded from mouse after daily exposures to 0, 10, 50 and 250 mGy γ-rays for 7 days. The supernatants from the twice-passaged CDCs were collected for measuring the concentration of VEGF ( A ) and IGF-1 ( B ) by ELISA. Values are the mean ± SD (n = 3).
    Figure Legend Snippet: Growth factors production of cardiac explant-derived cells (CDCs). CDCs were expanded from mouse after daily exposures to 0, 10, 50 and 250 mGy γ-rays for 7 days. The supernatants from the twice-passaged CDCs were collected for measuring the concentration of VEGF ( A ) and IGF-1 ( B ) by ELISA. Values are the mean ± SD (n = 3).

    Techniques Used: Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Recovery on the production of growth factors from cardiac explant-derived cells (CDCs) after radiation exposure. CDCs were expanded from mouse at 1 and 3 weeks after a single exposure to 3 Gy γ-rays. The supernatants from the twice-passaged CDCs were collected for measuring the concentration of VEGF ( A ) and IGF-1 ( B ) by ELISA. Values are the mean ± SD (n = 3).
    Figure Legend Snippet: Recovery on the production of growth factors from cardiac explant-derived cells (CDCs) after radiation exposure. CDCs were expanded from mouse at 1 and 3 weeks after a single exposure to 3 Gy γ-rays. The supernatants from the twice-passaged CDCs were collected for measuring the concentration of VEGF ( A ) and IGF-1 ( B ) by ELISA. Values are the mean ± SD (n = 3).

    Techniques Used: Derivative Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Related Articles

    Incubation:

    Article Title: Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow microenvironment via tumor cyclooxygenase-2
    Article Snippet: Nonspecific binding sites on plastic were blocked by treating the wells with 100 μl of PBS containing 0.5% BSA for 2 h at room temperature. .. In some experiments, B16M cells were incubated with either basal medium, or two different concentrations of PGE2, 10 and 100 ng/ml (Sigma Chemicals, St. Louis, MO) for 2 h, or with 1 μg/ml celecoxib for 30 min before addition of 100 ng/ml recombinant mouse VEGF (R & D Systems, Minneapolis, MN). .. In other experiments, A375M cells were preincubated with or without 1 μg/ml celecoxib for 30 min before addition of basal medium, 6 h-untreated or LPS-treated BMSC-CM, and 10 ng/ml recombinant human VEGF (R & D Systems, Minneapolis, MN) for other 4 h. Then, B16M or A375M cells were BCECF-AM-labeled and after washing, they were added (5 × 104 cells/well) to quadruplicate wells.

    Recombinant:

    Article Title: Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow microenvironment via tumor cyclooxygenase-2
    Article Snippet: Nonspecific binding sites on plastic were blocked by treating the wells with 100 μl of PBS containing 0.5% BSA for 2 h at room temperature. .. In some experiments, B16M cells were incubated with either basal medium, or two different concentrations of PGE2, 10 and 100 ng/ml (Sigma Chemicals, St. Louis, MO) for 2 h, or with 1 μg/ml celecoxib for 30 min before addition of 100 ng/ml recombinant mouse VEGF (R & D Systems, Minneapolis, MN). .. In other experiments, A375M cells were preincubated with or without 1 μg/ml celecoxib for 30 min before addition of basal medium, 6 h-untreated or LPS-treated BMSC-CM, and 10 ng/ml recombinant human VEGF (R & D Systems, Minneapolis, MN) for other 4 h. Then, B16M or A375M cells were BCECF-AM-labeled and after washing, they were added (5 × 104 cells/well) to quadruplicate wells.

    Article Title: Angiogenesis is inhibitory for mammalian digit regeneration
    Article Snippet: Affi‐Gel Blue Gel beads (150–200 μm in diameter, Bio‐Rad, Hercules, CA) were pre‐selected and soaked in different concentrations of growth factor diluted in phosphate‐buffered saline (PBS) with 0.1% BSA. .. Growth factors used included recombinant human BMP9, recombinant mouse VEGF‐A (VEGF) and recombinant human Serpin F1/PEDF (R & D Systems, Minneapolis, MN). ..

    Article Title: Inhibiting NF-?B in the developing lung disrupts angiogenesis and alveolarization
    Article Snippet: .. After starvation, the media was replaced with starvation media containing vehicle control (DMSO, 1:40,000) or BAY (2.5 μM), in the presence or absence of recombinant mouse VEGF-A (50 ng/ml) (R & D Systems), and the cells were counted at 48 h using a hemocytometer ( ). ..

    Article Title: Deciphering the Role of Emx1 in Neurogenesis: A Neuroproteomics Approach
    Article Snippet: BrdU and proliferation assay NSCs were plated onto poly-L-ornithine coated plates at 105 /well (24-well plate). .. Twenty-four hours post plating, recombinant mouse VEGF-A (100 ng/ml, R & D system) was added to culture medium and BrdU was added into culture 24 h later at a concentration of 10 μM prior to fixation with 4% PFA. ..

    Article Title: Increased supply from blood vessels promotes the activation of dormant primordial follicles in mouse ovaries
    Article Snippet: .. To confirm this hypothesis, we analyzed the expression of KITL in the ovaries that were transplanted with a biodegradable gel containing 10 μg/mL recombinant VEGF and in cultured P0 mouse ovaries ( ). .. These results indicated that the increased number of blood vessels and the supply of serum components promoted the expression of KITL in primordial follicles ( ).

    Article Title: Proteolytic Cleavages in the VEGF Family: Generating Diversity among Angiogenic VEGFs, Essential for the Activation of Lymphangiogenic VEGFs
    Article Snippet: While it is possible to predict the signal peptidase’s likely cleavage position, only N-terminal sequencing can give a definite answer. .. Many of the early experiments involving recombinant VEGF-D have used a truncated cDNA that results in a VEGF-D form, which is an intermediate between the VEGFR-2-monospecific and the VEGFR-2-/VEGFR-3-bispecific endogenous VEGF-D forms, making it difficult to interpret the data [ ]. .. However, after the recent identification of the cleaving proteases, it became possible to generate specific mature forms by co-transfection of the protease with the full-length wildtype growth factor cDNA [ , ].

    Article Title: VEGF Secreted by Hypoxic Müller Cells Induces MMP-2 Expression and Activity in Endothelial Cells to Promote Retinal Neovascularization in Proliferative Diabetic Retinopathy
    Article Snippet: .. Recombinant mouse VEGF (rmVEGF), recombinant human VEGF (rhVEGF), MMP-2, and VEGF ELISA kits were obtained from R & D Systems. .. Predesigned control (scrambled) and VEGF small interfering RNA sequences were obtained from Qiagen.

    Article Title: Trehalose 6,6?-Dimycolate (Cord Factor) Enhances Neovascularization through Vascular Endothelial Growth Factor Production by Neutrophils and Macrophages
    Article Snippet: Some mice were subjected to an intraperitoneal injection of 20 μg of goat anti-mouse VEGF antibody (R & D Systems, Minneapolis, Minn.) or 20 μg of goat immunoglobulin G (IgG; Southern Biotechnology Associates, Birmingham, United Kingdom) as a control 1 day before the pouch formation. .. In some FIA-alone-injected pouches, 0.1 or 1 μg of recombinant mouse VEGF (R & D Systems) was injected soon after FIA injection. .. Paraffin sections were stained with hematoxylin and eosin (H & E).

    Concentration Assay:

    Article Title: Deciphering the Role of Emx1 in Neurogenesis: A Neuroproteomics Approach
    Article Snippet: BrdU and proliferation assay NSCs were plated onto poly-L-ornithine coated plates at 105 /well (24-well plate). .. Twenty-four hours post plating, recombinant mouse VEGF-A (100 ng/ml, R & D system) was added to culture medium and BrdU was added into culture 24 h later at a concentration of 10 μM prior to fixation with 4% PFA. ..

    Expressing:

    Article Title: Increased supply from blood vessels promotes the activation of dormant primordial follicles in mouse ovaries
    Article Snippet: .. To confirm this hypothesis, we analyzed the expression of KITL in the ovaries that were transplanted with a biodegradable gel containing 10 μg/mL recombinant VEGF and in cultured P0 mouse ovaries ( ). .. These results indicated that the increased number of blood vessels and the supply of serum components promoted the expression of KITL in primordial follicles ( ).

    Cell Culture:

    Article Title: Increased supply from blood vessels promotes the activation of dormant primordial follicles in mouse ovaries
    Article Snippet: .. To confirm this hypothesis, we analyzed the expression of KITL in the ovaries that were transplanted with a biodegradable gel containing 10 μg/mL recombinant VEGF and in cultured P0 mouse ovaries ( ). .. These results indicated that the increased number of blood vessels and the supply of serum components promoted the expression of KITL in primordial follicles ( ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: VEGF Secreted by Hypoxic Müller Cells Induces MMP-2 Expression and Activity in Endothelial Cells to Promote Retinal Neovascularization in Proliferative Diabetic Retinopathy
    Article Snippet: .. Recombinant mouse VEGF (rmVEGF), recombinant human VEGF (rhVEGF), MMP-2, and VEGF ELISA kits were obtained from R & D Systems. .. Predesigned control (scrambled) and VEGF small interfering RNA sequences were obtained from Qiagen.

    Injection:

    Article Title: Trehalose 6,6?-Dimycolate (Cord Factor) Enhances Neovascularization through Vascular Endothelial Growth Factor Production by Neutrophils and Macrophages
    Article Snippet: Some mice were subjected to an intraperitoneal injection of 20 μg of goat anti-mouse VEGF antibody (R & D Systems, Minneapolis, Minn.) or 20 μg of goat immunoglobulin G (IgG; Southern Biotechnology Associates, Birmingham, United Kingdom) as a control 1 day before the pouch formation. .. In some FIA-alone-injected pouches, 0.1 or 1 μg of recombinant mouse VEGF (R & D Systems) was injected soon after FIA injection. .. Paraffin sections were stained with hematoxylin and eosin (H & E).

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    R&D Systems vegf quantikine elisa kit
    Comparison of <t>VEGF</t> expression across healthy adult ocular tissues. ( A and B ) VEGF protein content in ocular tissues from wild-type CD1 mice, determined by <t>ELISA;</t> n = 4 mice each; data are shown as mean ± SD per milliliter lysate ( A ) or as mean ± SD proportion of total protein in the lysates ( B ). ( C–E ) Vegfa transcript levels in ocular tissues from wild-type CD1 mice, determined by qRT-PCR; n = 4 mice each. ( C ) Total Vegfa expression for each tissue relative to expression in the cornea (mean ± SD). ( D ) Schematic representation of the exon structure of the 3 major mouse Vegfa isoform transcripts, including the alternatively spliced domains that encode the extracellular matrix–binding domains (yellow); the positions of the isoform-selective oligonucleotide primers used are indicated arrows, and the amplified region by dashed lines. ( E ) Proportions of Vegfa isoforms (mean; only the negative SD arm is shown). Each data point represents the value for pooled tissues from both eyes of 1 mouse ( A and B ) or the tissue from 1 eye of 1 mouse ( C ).
    Vegf Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf quantikine elisa kit/product/R&D Systems
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    R&D Systems human vegf quantikine elisa kits
    NK-B down-regulates VEGFRs. (A) YSECs and HUVECs on tissue culture plates were cultured in medium lacking supplement for 14 h and treated with indicated reagents in supplement-containing medium for 24 h, and <t>VEGF</t> secreted in the culture medium was quantitated by <t>ELISA</t> (two [right] to three [left] independent experiments). Sup, supplemented M200 medium. (A and B) Statistical significance was determined relative to the supplement condition (*, P
    Human Vegf Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human vegf quantikine elisa kits/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86
    R&D Systems mouse vegf duoset elisa kit
    Elevated vascular endothelial growth factor ( <t>VEGF</t> ) in acyloxyacyl hydrolase (AOAH)-deficient bladders. A : VEGF was quantified in bladder homogenates by <t>ELISA.</t> Bladders were harvested from B6 mice, uninfected or infected with pseudorabies virus (PRV) to induce neurogenic cystitis or from AOAH-deficient mice. Bladder VEGF was significantly elevated in PRV-treated and AOAH-deficient mice relative to untreated B6 mice (* P
    Mouse Vegf Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse vegf duoset elisa kit/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    97
    R&D Systems human vegf duoset elisa kit
    In vivo effects of pK1-5 on <t>VEGF</t> expression. Mice with subcutaneous tumours were treated with NaCl ( n = 5), DOTAP ( n = 6), pMock ( n = 6), or pK1-5 ( n = 5) and sacrificed after ten days. Tumours and livers were explanted and homogenized, serum was collected, and protein concentrations were evaluated. VEGF levels were quantified with a murine VEGF <t>ELISA.</t> Data are presented as mean pg VEGF ± SEM. * P
    Human Vegf Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human vegf duoset elisa kit/product/R&D Systems
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    Comparison of VEGF expression across healthy adult ocular tissues. ( A and B ) VEGF protein content in ocular tissues from wild-type CD1 mice, determined by ELISA; n = 4 mice each; data are shown as mean ± SD per milliliter lysate ( A ) or as mean ± SD proportion of total protein in the lysates ( B ). ( C–E ) Vegfa transcript levels in ocular tissues from wild-type CD1 mice, determined by qRT-PCR; n = 4 mice each. ( C ) Total Vegfa expression for each tissue relative to expression in the cornea (mean ± SD). ( D ) Schematic representation of the exon structure of the 3 major mouse Vegfa isoform transcripts, including the alternatively spliced domains that encode the extracellular matrix–binding domains (yellow); the positions of the isoform-selective oligonucleotide primers used are indicated arrows, and the amplified region by dashed lines. ( E ) Proportions of Vegfa isoforms (mean; only the negative SD arm is shown). Each data point represents the value for pooled tissues from both eyes of 1 mouse ( A and B ) or the tissue from 1 eye of 1 mouse ( C ).

    Journal: JCI Insight

    Article Title: VEGF188 promotes corneal reinnervation after injury

    doi: 10.1172/jci.insight.130979

    Figure Lengend Snippet: Comparison of VEGF expression across healthy adult ocular tissues. ( A and B ) VEGF protein content in ocular tissues from wild-type CD1 mice, determined by ELISA; n = 4 mice each; data are shown as mean ± SD per milliliter lysate ( A ) or as mean ± SD proportion of total protein in the lysates ( B ). ( C–E ) Vegfa transcript levels in ocular tissues from wild-type CD1 mice, determined by qRT-PCR; n = 4 mice each. ( C ) Total Vegfa expression for each tissue relative to expression in the cornea (mean ± SD). ( D ) Schematic representation of the exon structure of the 3 major mouse Vegfa isoform transcripts, including the alternatively spliced domains that encode the extracellular matrix–binding domains (yellow); the positions of the isoform-selective oligonucleotide primers used are indicated arrows, and the amplified region by dashed lines. ( E ) Proportions of Vegfa isoforms (mean; only the negative SD arm is shown). Each data point represents the value for pooled tissues from both eyes of 1 mouse ( A and B ) or the tissue from 1 eye of 1 mouse ( C ).

    Article Snippet: The VEGF Quantikine ELISA kit (R & D Systems) was used to determine VEGF content, and the Bradford assay (Bio-Rad) was used to determine total protein concentration of the tissue lysates.

    Techniques: Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Binding Assay, Amplification

    NK-B down-regulates VEGFRs. (A) YSECs and HUVECs on tissue culture plates were cultured in medium lacking supplement for 14 h and treated with indicated reagents in supplement-containing medium for 24 h, and VEGF secreted in the culture medium was quantitated by ELISA (two [right] to three [left] independent experiments). Sup, supplemented M200 medium. (A and B) Statistical significance was determined relative to the supplement condition (*, P

    Journal: The Journal of Cell Biology

    Article Title: An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis

    doi: 10.1083/jcb.200603152

    Figure Lengend Snippet: NK-B down-regulates VEGFRs. (A) YSECs and HUVECs on tissue culture plates were cultured in medium lacking supplement for 14 h and treated with indicated reagents in supplement-containing medium for 24 h, and VEGF secreted in the culture medium was quantitated by ELISA (two [right] to three [left] independent experiments). Sup, supplemented M200 medium. (A and B) Statistical significance was determined relative to the supplement condition (*, P

    Article Snippet: VEGF ELISA YSECs and HUVECs were cultured in 6-well plates (3 × 105 cells/well), incubated in supplement-free M200 medium overnight, and treated with vehicle, NK-B, IBMX, NK-B/IBMX, U46619, NK-B/U46619, or BAPTA/AM in supplement-containing medium for 24 h. Secreted VEGF was quantitated with mouse or human VEGF Quantikine ELISA kits (R & D Systems) according to the manufacturer's instructions.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Elevated vascular endothelial growth factor ( VEGF ) in acyloxyacyl hydrolase (AOAH)-deficient bladders. A : VEGF was quantified in bladder homogenates by ELISA. Bladders were harvested from B6 mice, uninfected or infected with pseudorabies virus (PRV) to induce neurogenic cystitis or from AOAH-deficient mice. Bladder VEGF was significantly elevated in PRV-treated and AOAH-deficient mice relative to untreated B6 mice (* P

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Acyloxyacyl hydrolase modulates pelvic pain severity

    doi: 10.1152/ajpregu.00239.2017

    Figure Lengend Snippet: Elevated vascular endothelial growth factor ( VEGF ) in acyloxyacyl hydrolase (AOAH)-deficient bladders. A : VEGF was quantified in bladder homogenates by ELISA. Bladders were harvested from B6 mice, uninfected or infected with pseudorabies virus (PRV) to induce neurogenic cystitis or from AOAH-deficient mice. Bladder VEGF was significantly elevated in PRV-treated and AOAH-deficient mice relative to untreated B6 mice (* P

    Article Snippet: Bladder VEGF was then quantified using a mouse VEGF DuoSet ELISA kit (R & D Systems).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay, Infection

    In vivo effects of pK1-5 on VEGF expression. Mice with subcutaneous tumours were treated with NaCl ( n = 5), DOTAP ( n = 6), pMock ( n = 6), or pK1-5 ( n = 5) and sacrificed after ten days. Tumours and livers were explanted and homogenized, serum was collected, and protein concentrations were evaluated. VEGF levels were quantified with a murine VEGF ELISA. Data are presented as mean pg VEGF ± SEM. * P

    Journal: BioMed Research International

    Article Title: Plasmid Transfer of Plasminogen K1-5 Reduces Subcutaneous Hepatoma Growth by Affecting Inflammatory Factors

    doi: 10.1155/2014/656527

    Figure Lengend Snippet: In vivo effects of pK1-5 on VEGF expression. Mice with subcutaneous tumours were treated with NaCl ( n = 5), DOTAP ( n = 6), pMock ( n = 6), or pK1-5 ( n = 5) and sacrificed after ten days. Tumours and livers were explanted and homogenized, serum was collected, and protein concentrations were evaluated. VEGF levels were quantified with a murine VEGF ELISA. Data are presented as mean pg VEGF ± SEM. * P

    Article Snippet: VEGF in HuH7 was quantified with the human VEGF DuoSet ELISA kit (R & D Systems, Wiesbaden, Germany).

    Techniques: In Vivo, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay