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R&D Systems pe conjugated anti mouse s1p1 edg
Pe Conjugated Anti Mouse S1p1 Edg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe conjugated anti mouse s1p1 edg/product/R&D Systems
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pe conjugated anti mouse s1p1 edg - by Bioz Stars, 2022-09
94/100 stars

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    R&D Systems s1pr1 pe
    Increased Fr. D and loss of Fr. F cells in bone marrow of Grk2 −/− mb1 -cre mice. ( A ) Flow cytometry results from analysis bone marrow cells from Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. ( B ) Flow cytometry results from bone marrow showing CD43 expression and the % Fr.A - Fr.F cells. ( C ) Flow cytometry of bone marrow cells gated on B220 + CD19 + CD43 − examining IgD versus IgM. ( D ) Flow cytometry results showing receptors expression and representative <t>S1PR1</t> histograms for each fraction. ( E ) Spontaneous and directed migration of Grk2 fl/fl and Grk2 fl/fl mb1-cre bone marrow B cell fractions to CXCL12 and S1P. All results from B cells purified from a minimum of 5 Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. Data shown as mean +/− standard error of the mean (p
    S1pr1 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s1pr1 pe/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s1pr1 pe - by Bioz Stars, 2022-09
    92/100 stars
      Buy from Supplier

    90
    R&D Systems mouse s1p1 edg 1 pe conjugated antibody
    The expression of molecules related to T cell migration after Fluvastatin treatment in vivo. C57BL/6 <t>mice</t> were intraperitoneally injected with Fluvastatin or an equal volume of buffer daily for 7 consecutive days before sacrificed. The expression of (a) CD62L, (b) CCR7, (c) <t>S1P1,</t> (d) CD44, and (e) CXCR3 on spleen and bone marrow T cells were detected by flow cytometry. (f) The proportion of CD3 + T cells in the spleen and bone marrow from buffer- and Fluvastatin-treated mice were explored by flow cytometry. Data represents the mean ± SD of results from 3 experiments ( n = 5 per group). ∗ P
    Mouse S1p1 Edg 1 Pe Conjugated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse s1p1 edg 1 pe conjugated antibody/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse s1p1 edg 1 pe conjugated antibody - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

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    Increased Fr. D and loss of Fr. F cells in bone marrow of Grk2 −/− mb1 -cre mice. ( A ) Flow cytometry results from analysis bone marrow cells from Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. ( B ) Flow cytometry results from bone marrow showing CD43 expression and the % Fr.A - Fr.F cells. ( C ) Flow cytometry of bone marrow cells gated on B220 + CD19 + CD43 − examining IgD versus IgM. ( D ) Flow cytometry results showing receptors expression and representative S1PR1 histograms for each fraction. ( E ) Spontaneous and directed migration of Grk2 fl/fl and Grk2 fl/fl mb1-cre bone marrow B cell fractions to CXCL12 and S1P. All results from B cells purified from a minimum of 5 Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. Data shown as mean +/− standard error of the mean (p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Biased S1PR1 Signaling in B cells Subverts Responses to Homeostatic Chemokines Severely Disorganizing Lymphoid Organ Architecture

    doi: 10.4049/jimmunol.1900678

    Figure Lengend Snippet: Increased Fr. D and loss of Fr. F cells in bone marrow of Grk2 −/− mb1 -cre mice. ( A ) Flow cytometry results from analysis bone marrow cells from Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. ( B ) Flow cytometry results from bone marrow showing CD43 expression and the % Fr.A - Fr.F cells. ( C ) Flow cytometry of bone marrow cells gated on B220 + CD19 + CD43 − examining IgD versus IgM. ( D ) Flow cytometry results showing receptors expression and representative S1PR1 histograms for each fraction. ( E ) Spontaneous and directed migration of Grk2 fl/fl and Grk2 fl/fl mb1-cre bone marrow B cell fractions to CXCL12 and S1P. All results from B cells purified from a minimum of 5 Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. Data shown as mean +/− standard error of the mean (p

    Article Snippet: Cells were washed stained with S1PR1-PE prior to incubation and at the indicated times.

    Techniques: Mouse Assay, Flow Cytometry, Expressing, Migration, Purification

    Increased non-specific migration, decreased CXCL13 but enhanced S1P triggered migration of Grk2 fl/fl mb1-cre B cells. ( A ) Chemotaxis assays to CXCL12 (ng/ml), CCL19 (ng/ml), CXCL13 (ng/ml), and S1P (nM). Shown on left, is non-specific migration, and on the right, specific migration of T1, FO, and MZ B cells. ( B ) Flow cytometry examining S1PR1, CXCR4, CXCR5, and CCR7 expression on B cell subsets from Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. FACS profile shown only for S1PR1. ( C ) Time dependent S1PR1 internalization following exposure to S1P (1 μM) and recovery of S1PR1 expression following placement of splenic B cells in media lacking S1P. N=3 vs. 3 ( Grk2 fl/fl , black lines; Grk2 fl/fl mb1-cre, grey lines). Experiments repeated 3–4 times. Data shown as mean +/− standard error of the mean (p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Biased S1PR1 Signaling in B cells Subverts Responses to Homeostatic Chemokines Severely Disorganizing Lymphoid Organ Architecture

    doi: 10.4049/jimmunol.1900678

    Figure Lengend Snippet: Increased non-specific migration, decreased CXCL13 but enhanced S1P triggered migration of Grk2 fl/fl mb1-cre B cells. ( A ) Chemotaxis assays to CXCL12 (ng/ml), CCL19 (ng/ml), CXCL13 (ng/ml), and S1P (nM). Shown on left, is non-specific migration, and on the right, specific migration of T1, FO, and MZ B cells. ( B ) Flow cytometry examining S1PR1, CXCR4, CXCR5, and CCR7 expression on B cell subsets from Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. FACS profile shown only for S1PR1. ( C ) Time dependent S1PR1 internalization following exposure to S1P (1 μM) and recovery of S1PR1 expression following placement of splenic B cells in media lacking S1P. N=3 vs. 3 ( Grk2 fl/fl , black lines; Grk2 fl/fl mb1-cre, grey lines). Experiments repeated 3–4 times. Data shown as mean +/− standard error of the mean (p

    Article Snippet: Cells were washed stained with S1PR1-PE prior to incubation and at the indicated times.

    Techniques: Migration, Chemotaxis Assay, Flow Cytometry, Expressing, Mouse Assay, FACS

    Leukocytosis, but a dearth of B cells in LNs and Peyer’s patches from Grk 2 fl/fl mb1-cre mice. ( A ) Flow cytometry analysis of the blood from control and Grk 2 fl/fl mb1-cre mice. Shown are total cell count and composition of blood leukocytes on a % basis. ( B ) Percentage of B cells in the indicated subsets from blood of Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( C ) Flow cytometry profiles of S1PR1 expression on indicated blood cells. (D) Flow cytometry results analyzing expression of S1PR1 and homing receptors on blood B cells. (E) Chemotaxis assays to CXCL12 (ng/ml), CCL19 (ng/ml), and CXCL13 (ng/ml). Shown on left, is non-specific migration, and on the right, specific migration of blood B, and CD4 T cells. ( F ) Cell recovery and subset distribution in LNs and Peyer’s patches (PP) from Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( G ) Spontaneous migration of LN B and CD4 T cells. ( H ) Specific migration of LN B and CD4 T cells to indicated chemokines (ng/ml) or S1P (nM), cells prepared from Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( I ) Flow cytometry results assessing S1PR1, CXCR4, CXCR5, and CCR7 expression on LN B cells from control and Grk 2 fl/fl mb1-cre mice. All results from B cells purified from a minimum of 5 Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. Data shown as mean +/− standard error of the mean (p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Biased S1PR1 Signaling in B cells Subverts Responses to Homeostatic Chemokines Severely Disorganizing Lymphoid Organ Architecture

    doi: 10.4049/jimmunol.1900678

    Figure Lengend Snippet: Leukocytosis, but a dearth of B cells in LNs and Peyer’s patches from Grk 2 fl/fl mb1-cre mice. ( A ) Flow cytometry analysis of the blood from control and Grk 2 fl/fl mb1-cre mice. Shown are total cell count and composition of blood leukocytes on a % basis. ( B ) Percentage of B cells in the indicated subsets from blood of Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( C ) Flow cytometry profiles of S1PR1 expression on indicated blood cells. (D) Flow cytometry results analyzing expression of S1PR1 and homing receptors on blood B cells. (E) Chemotaxis assays to CXCL12 (ng/ml), CCL19 (ng/ml), and CXCL13 (ng/ml). Shown on left, is non-specific migration, and on the right, specific migration of blood B, and CD4 T cells. ( F ) Cell recovery and subset distribution in LNs and Peyer’s patches (PP) from Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( G ) Spontaneous migration of LN B and CD4 T cells. ( H ) Specific migration of LN B and CD4 T cells to indicated chemokines (ng/ml) or S1P (nM), cells prepared from Grk 2 fl/fl and Grk 2 fl/fl mb1-cre mice. ( I ) Flow cytometry results assessing S1PR1, CXCR4, CXCR5, and CCR7 expression on LN B cells from control and Grk 2 fl/fl mb1-cre mice. All results from B cells purified from a minimum of 5 Grk2 fl/fl and Grk2 fl/fl mb1-cre mice. Data shown as mean +/− standard error of the mean (p

    Article Snippet: Cells were washed stained with S1PR1-PE prior to incubation and at the indicated times.

    Techniques: Mouse Assay, Flow Cytometry, Cell Counting, Expressing, Chemotaxis Assay, Migration, Purification

    The loss of GRK2 in B cells enhances S1P signaling but decreases CXCL13 signaling except for the intracellular Ca 2+ response. ( A ) Chemoattractant induced pAKT and pERK in B cell subsets. Grk2 fl/fl or Grk2 fl/fl mb1-cre B cells were exposed to CXCL13 (1000 ng/ml), or S1P (1μM) for indicated times and level of pAKT or pERK determined by flow cytometry. Data expressed as % of basal. ( B ) pERM levels in blood and splenic B cells from Grk2 fl/fl or Grk2 fl/fl mb1-cre mice. Level of pERM in blood B cell subsets and CD4 T cells determined by flow cytometry (left). Basal pERM levels in splenic B cell subsets and following exposure S1P (1μM). ( C ) Peak intracellular Ca 2+ levels following chemoattractant exposure and impact of pertussis toxin (200nM). Grk2 fl/fl or Grk2 fl/fl mb1-cre splenic B cells were exposed to varying concentration of CXCL13 (ng/ml) or S1P (nM). ( D ) Effect of a S1PR1 antagonist on S1P and CXCL13 induced increases in intracellular Ca 2+ . Plot of intracellular Ca 2+ levels over time in splenic B cells from Grk2 fl/fl or Grk2 fl/fl mb1-cre mice and exposed to S1P or CXCL13. Where indicated the B cells were pre-incubated with Ex 26 for 1 hours prior to the addition of S1P or CXCL13. Results are from 2–10 mice per group. All experiments repeated a minimum of three times. Data shown as mean +/− standard error of the mean (p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Biased S1PR1 Signaling in B cells Subverts Responses to Homeostatic Chemokines Severely Disorganizing Lymphoid Organ Architecture

    doi: 10.4049/jimmunol.1900678

    Figure Lengend Snippet: The loss of GRK2 in B cells enhances S1P signaling but decreases CXCL13 signaling except for the intracellular Ca 2+ response. ( A ) Chemoattractant induced pAKT and pERK in B cell subsets. Grk2 fl/fl or Grk2 fl/fl mb1-cre B cells were exposed to CXCL13 (1000 ng/ml), or S1P (1μM) for indicated times and level of pAKT or pERK determined by flow cytometry. Data expressed as % of basal. ( B ) pERM levels in blood and splenic B cells from Grk2 fl/fl or Grk2 fl/fl mb1-cre mice. Level of pERM in blood B cell subsets and CD4 T cells determined by flow cytometry (left). Basal pERM levels in splenic B cell subsets and following exposure S1P (1μM). ( C ) Peak intracellular Ca 2+ levels following chemoattractant exposure and impact of pertussis toxin (200nM). Grk2 fl/fl or Grk2 fl/fl mb1-cre splenic B cells were exposed to varying concentration of CXCL13 (ng/ml) or S1P (nM). ( D ) Effect of a S1PR1 antagonist on S1P and CXCL13 induced increases in intracellular Ca 2+ . Plot of intracellular Ca 2+ levels over time in splenic B cells from Grk2 fl/fl or Grk2 fl/fl mb1-cre mice and exposed to S1P or CXCL13. Where indicated the B cells were pre-incubated with Ex 26 for 1 hours prior to the addition of S1P or CXCL13. Results are from 2–10 mice per group. All experiments repeated a minimum of three times. Data shown as mean +/− standard error of the mean (p

    Article Snippet: Cells were washed stained with S1PR1-PE prior to incubation and at the indicated times.

    Techniques: Flow Cytometry, Mouse Assay, Concentration Assay, Incubation

    The expression of molecules related to T cell migration after Fluvastatin treatment in vivo. C57BL/6 mice were intraperitoneally injected with Fluvastatin or an equal volume of buffer daily for 7 consecutive days before sacrificed. The expression of (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 on spleen and bone marrow T cells were detected by flow cytometry. (f) The proportion of CD3 + T cells in the spleen and bone marrow from buffer- and Fluvastatin-treated mice were explored by flow cytometry. Data represents the mean ± SD of results from 3 experiments ( n = 5 per group). ∗ P

    Journal: BioMed Research International

    Article Title: Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2

    doi: 10.1155/2020/7619849

    Figure Lengend Snippet: The expression of molecules related to T cell migration after Fluvastatin treatment in vivo. C57BL/6 mice were intraperitoneally injected with Fluvastatin or an equal volume of buffer daily for 7 consecutive days before sacrificed. The expression of (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 on spleen and bone marrow T cells were detected by flow cytometry. (f) The proportion of CD3 + T cells in the spleen and bone marrow from buffer- and Fluvastatin-treated mice were explored by flow cytometry. Data represents the mean ± SD of results from 3 experiments ( n = 5 per group). ∗ P

    Article Snippet: Chemokine receptors mAbs anti-mCCR7-eFlour450 (#48-1971-82, eBioscience), anti-mCXCR3-PE-Cy7 (#25-1831-82, eBioscience), and anti-S1P1 (PE, # FAB7089P, R & D Systems) were used for T cell phenotype detection.

    Techniques: Expressing, Migration, In Vivo, Mouse Assay, Injection, Flow Cytometry

    The expression of surface molecules related to T cell migration on T cells in transplanted recipients. BALB/c mice were transplanted with bone marrow plus T cells from Fluvastatin or buffer pretreated C57BL/6 donors. Single-cell suspension of the thymus, spleen, lymph nodes, and blood were acquired from each recipient on days 3, 7, 14, 21, and day 28 posttransplantation and subsequently gated on CD3 + T cells by flow cytometry to analyze (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 expression. Data represents the mean ± SD of results from 3 independent experiments. There were 4-5 mice in each time point in Fluvastatin and buffer GVHD groups, respectively, and 4 mice were sacrificed in unmanipulated group. ∗ P

    Journal: BioMed Research International

    Article Title: Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2

    doi: 10.1155/2020/7619849

    Figure Lengend Snippet: The expression of surface molecules related to T cell migration on T cells in transplanted recipients. BALB/c mice were transplanted with bone marrow plus T cells from Fluvastatin or buffer pretreated C57BL/6 donors. Single-cell suspension of the thymus, spleen, lymph nodes, and blood were acquired from each recipient on days 3, 7, 14, 21, and day 28 posttransplantation and subsequently gated on CD3 + T cells by flow cytometry to analyze (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 expression. Data represents the mean ± SD of results from 3 independent experiments. There were 4-5 mice in each time point in Fluvastatin and buffer GVHD groups, respectively, and 4 mice were sacrificed in unmanipulated group. ∗ P

    Article Snippet: Chemokine receptors mAbs anti-mCCR7-eFlour450 (#48-1971-82, eBioscience), anti-mCXCR3-PE-Cy7 (#25-1831-82, eBioscience), and anti-S1P1 (PE, # FAB7089P, R & D Systems) were used for T cell phenotype detection.

    Techniques: Expressing, Migration, Mouse Assay, Flow Cytometry

    Activation of the FoxO1–KLF2–S1P1 axis by CCR2 signaling through Stat3. (A) Surface expression of S1P1 in the CD69 + Qa-2 − (SP1 + SP2), CD69 − Qa-2 − (SP3), and CD69 − Qa-2 + (SP4) subsets of CD4 SP thymocytes from WT and KO mice. The experiments were repeated three times with six mice for each group. Representative dot plots are shown. (B) Percentage of S1P1 + cells are presented as mean ± SD. (C) Foxo1, Klf2, and S1pr1 mRNA expression in WT and KO CD4 SP thymocytes as determined by qPCR. The experiments were repeated three times with triplicates. Data are present as mean ± SEM. (D) CD69 − Qa-2 + SP4 thymocytes from WT and KO mice were stimulated with CCL2 in the presence or absence of STAT3 inhibitor Stattic. Foxo1, Klf2, and S1pr1 mRNA expression was detected by qPCR. The experiments were repeated three times with triplicates. Data are present as mean ± SEM. (E) Following stimulation with CCL2, WT SP4 thymocytes were examined for surface expression of S1P1. Representative histograms are shown out of three independent experiments. (F,G) Levels of phosphorylated Stat3 were examined by Western blotting (F) or intracellular staining (G) in SP4 thymocytes after stimulation with CCL2. Western blotting was performed at 30 min after stimulation. The experiments were repeated three times with similar results. (H–J) SP4 thymocytes were stimulated with CCL2 in the presence or absence of STAT3 inhibitor Stattic. Surface expression of S1P1 was assayed by flow cytometry (H) . Subcellular localization of FoxO1 was revealed by immunofluorescent staining (I) and nucleus Foxo1 was quantified by mean fluorescent intensity (MFI) (J) . Data are presented as mean ± SD. Two independent experiments were performed with similar results. * p

    Journal: Frontiers in Immunology

    Article Title: CCR2 Signal Facilitates Thymic Egress by Priming Thymocyte Responses to Sphingosine-1-Phosphate

    doi: 10.3389/fimmu.2018.01263

    Figure Lengend Snippet: Activation of the FoxO1–KLF2–S1P1 axis by CCR2 signaling through Stat3. (A) Surface expression of S1P1 in the CD69 + Qa-2 − (SP1 + SP2), CD69 − Qa-2 − (SP3), and CD69 − Qa-2 + (SP4) subsets of CD4 SP thymocytes from WT and KO mice. The experiments were repeated three times with six mice for each group. Representative dot plots are shown. (B) Percentage of S1P1 + cells are presented as mean ± SD. (C) Foxo1, Klf2, and S1pr1 mRNA expression in WT and KO CD4 SP thymocytes as determined by qPCR. The experiments were repeated three times with triplicates. Data are present as mean ± SEM. (D) CD69 − Qa-2 + SP4 thymocytes from WT and KO mice were stimulated with CCL2 in the presence or absence of STAT3 inhibitor Stattic. Foxo1, Klf2, and S1pr1 mRNA expression was detected by qPCR. The experiments were repeated three times with triplicates. Data are present as mean ± SEM. (E) Following stimulation with CCL2, WT SP4 thymocytes were examined for surface expression of S1P1. Representative histograms are shown out of three independent experiments. (F,G) Levels of phosphorylated Stat3 were examined by Western blotting (F) or intracellular staining (G) in SP4 thymocytes after stimulation with CCL2. Western blotting was performed at 30 min after stimulation. The experiments were repeated three times with similar results. (H–J) SP4 thymocytes were stimulated with CCL2 in the presence or absence of STAT3 inhibitor Stattic. Surface expression of S1P1 was assayed by flow cytometry (H) . Subcellular localization of FoxO1 was revealed by immunofluorescent staining (I) and nucleus Foxo1 was quantified by mean fluorescent intensity (MFI) (J) . Data are presented as mean ± SD. Two independent experiments were performed with similar results. * p

    Article Snippet: Anti-CCR2-APC (Catalog # FAB5538A) and anti-S1P1-PE (Catalog # FAB7089P) were purchased from R & D Systems (Minneapolis, MN, USA).

    Techniques: Activation Assay, Expressing, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot, Staining, Flow Cytometry