Journal: BioMed Research International
Article Title: Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2
Figure Lengend Snippet: The expression of surface molecules related to T cell migration on T cells in transplanted recipients. BALB/c mice were transplanted with bone marrow plus T cells from Fluvastatin or buffer pretreated C57BL/6 donors. Single-cell suspension of the thymus, spleen, lymph nodes, and blood were acquired from each recipient on days 3, 7, 14, 21, and day 28 posttransplantation and subsequently gated on CD3 + T cells by flow cytometry to analyze (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 expression. Data represents the mean ± SD of results from 3 independent experiments. There were 4-5 mice in each time point in Fluvastatin and buffer GVHD groups, respectively, and 4 mice were sacrificed in unmanipulated group. ∗ P
Article Snippet: Chemokine receptors mAbs anti-mCCR7-eFlour450 (#48-1971-82, eBioscience), anti-mCXCR3-PE-Cy7 (#25-1831-82, eBioscience), and anti-S1P1 (PE, # FAB7089P, R & D Systems) were used for T cell phenotype detection.
Techniques: Expressing, Migration, Mouse Assay, Flow Cytometry