Article Title: The role of voltage-gated chloride channels in the epileptogenesis of temporal lobe epilepsy
Figure Lengend Snippet: The expression of ClC-3 was increased in the ipsilateral hippocampus of chronic TLE mice . (a) Representative double immunofluorescence images showing the neuronal co-expression of ClC-3 and NeuN in the hippocampal CA1 region of control and chronic TLE mice. Scale bar, 20 μm. Images were selected from three mice for each group. (b) The ClC-3 protein in the homogenates of hippocampus from control and TLE mice was detected at 72-95 kDa by Western blot. (c) Densitometric analysis results showing the increased expression of ClC-3 in the hippocampus of the chronic TLE mice. (** P
Article Snippet: The membranes were blocked by 5% BSA at room temperature for one hour and incubated with the rabbit anti-ClC-3 (Alomone Labs Cat# ACL-001, RRID: AB_2039814) and rabbit anti-GAPDH (Sigma-Aldrich Cat# G9545, RRID: AB_796208) antibodies overnight at 4°C.
Techniques: Expressing, Mouse Assay, Immunofluorescence, Western Blot