mouse monoclonal substance p antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Lumbar endplate microfracture injury induces Modic-like changes, intervertebral disc degeneration and spinal cord sensitization – An In Vivo Rat Model

doi: 10.1101/2023.01.27.525924

Figure Lengend Snippet: Spinal Cord sensitization from EP injury. A) Immunofluorescence images of spinal cord (SC) with outlined gray matter showing Neurons (red) and presence of substance P (green) in different groups with focus on dorsal horns. B) hematoxylin and eosin staining of spinal cord with outline of grey matter. C) quantification of SubP in dorsal horn. ** indicate significant difference between groups with p<0.01.

Article Snippet: Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486, Abcam) as negative control [ ].

Techniques: Immunofluorescence, Staining

Journal: Nature Communications

Article Title: Parabrachial-to-parasubthalamic nucleus pathway mediates fear-induced suppression of feeding in male mice

doi: 10.1038/s41467-022-35634-2

Figure Lengend Snippet: a – e PACAP, Tac1, and CRH immune-positive neurons in the PSTN. Scale bars represent 200 μm. cp, cerebral peduncle. Experiments were repeated independently in at least four mice with similar results. f Quantification of the percentage of PACAP neurons co-expressing Tac1 or CRH ( n = 4 images). It was calculated as the ratio of the number of cells (double-positive cells/PACAP-positive cells). g Schematic of the electrophysiological analysis and representative traces of EPSCs (gray, 15 consecutive responses; red, average) evoked by photo-stimulation (every 20 s, 5-ms duration). Scale bar, 100 pA and 50 ms. h Proportion of the recorded PACAP PSTN neurons with EPSCs (EPSC–, n = 3 cells; EPSC+, n = 13 cells) and summary of light-evoked EPSC amplitudes ( n = 16 cells). Data are represented as mean ± SEM.

Article Snippet: As primary antibodies, a rabbit polyclonal antibody against PACAP-38 (1:200, BMA, Rheinstrasse, Switzerland; T-4473), a mouse monoclonal antibody against Substance P/Tac1 (1:200, abcam, Cambridge, UK; ab14184), and a guinea pig polyclonal antibody against CRF (1:300, BMA, Rheinstrasse, Switzerland; T-5007) were used.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Infants and the Significance in Viral Pathogenesis

doi: 10.1371/journal.pone.0083766

Figure Lengend Snippet: (a) The fold-change of the TAC1 gene expression in the PBMCs from EV71-infected rhesus monkey infants according to microarray analysis. The color scale indicates the levels of gene expression from low (green, blue and purple) to high (red, pink and yellow). The values are shown on a log 2 scale. (b) The levels of TAC1 gene expression in the thalamus and spinal cord from the EV71-infected rhesus monkey infants were measured by real-time qPCR. The y-axis indicates the relative quantity of the TAC1 mRNA in the samples compared with the control (Y = 1), and normalized to the endogenous GAPDH expression level. The samples were collected at days 4, 7 and 10 p.i.. Individual detection was performed in triplicate. Error bars are presented as the mean±SD. (c) The expression of Substance P in the thalamus and spinal cord were measured by immunohistochemical assay. The samples were tested using an anti-substance P antibody and an HRP-conjugated anti-mouse IgG antibody. Images are shown at 200× magnification.

Article Snippet: Substance P and IL-17 were detected using a mouse anti-substance P monoclonal antibody (Abcam, Cambridge, UK), anti-IL17A antibody (eBioscience, San Diego, CA, USA) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibodies (Sigma, Deisenhofen, Germany) followed by color development with diaminobenzidine to detect the antigen-antibody reaction.

Techniques: Expressing, Infection, Microarray, Immunohistochemical staining

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: TaqMan probes used for quantitative polymerase chain reaction analysis

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: Real-time Polymerase Chain Reaction

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: Relative mRNA expression. In diabetes (DM) rats, the M3 mRNA expression was increased at 4 wk compared with 12 wk and the M2 / M3 mRNA expression at 4 wk was increased compared with normal diet (ND)/high-fat diet (HFD) control groups. The M3 mRNA recovered at 4 wk after insulin treatment. The DM rats had significantly decreased mRNA expression of nerve growth factor ( NGF ) at 4 and 12 wk and substance P at 4 wk compared with ND/HFD controls. Insulin treatment can recover the mRNA expression of NGF and substance P , and hAFSCs treatment can recover the mRNA expression of NGF at 4 wk after DM induction. n = 6. Statistics: Median with first and third quartile (Q1, Q3) for continuous variables. Two-way analysis of variance was used for initial analysis. Kruskal-Wallis test with post hoc Bonferroni test for intergroup analysis. Mann-Whitney U test for the comparison between 4 wk and 12 wk. P values are shown in each comparison. A: M2-muscarinic receptor; B: M3-muscarinic receptor; C: Nerve growth factor; D: Calcitonin gene-related peptide; E: Substance P in the bladder of rats of different groups. ND: Normal diet; DM: Diabetes mellitus; hAFSCs: Human amniotic fluid stem cells; HFD: High-fat diet.

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: Expressing, MANN-WHITNEY

Journal: World Journal of Stem Cells

Article Title: Human amniotic fluid stem cell therapy can help regain bladder function in type 2 diabetic rats

doi: 10.4252/wjsc.v14.i5.330

Figure Lengend Snippet: Immunoreactivities. In diabetes (DM) rats, the M2 immunoreactivity was increased at 4 wk compared with 12 wk. The M2 immunoreactivity was increased at 4 and 12 wk and M3 immunoreactivity was increased at 4 wk compared with normal diet (ND)/high-fat diet (HFD) control groups. The M2 immunoreactivity recovered at 4 wk after insulin treatment. The DM rats had significantly reduced immunoreactivities to nerve growth factor (NGF), calcitonin gene-related peptide (CGRP), and substance P at 4 and 12 wk compared with ND/HFD control groups. Insulin treatment can recover CGRP immunoreactivity and human amniotic fluid stem cells treatment can recover NGF immunoreactivity at 4 wk after DM induction. n = 6. Statistics: Median with first and third quartile (Q1, Q3) for continuous variables. Two-way analysis of variance was used for initial analysis. Kruskal-Wallis test with post hoc Bonferroni test for intergroup analysis. Mann-Whitney U test for the comparison between 4 wk and 12 wk. P values are shown in each comparison. A: M2-muscarinic receptor; B: M3-muscarinic receptor; C: Nerve growth factor; D: Calcitonin gene-related peptide; E: Substance P in the bladder of rats of different groups. ND: Normal diet; DM: Diabetes mellitus; hAFSCs: Human amniotic fluid stem cells; HFD: High-fat diet.

Article Snippet: After blocking with Dako REAL peroxidase blocking solution (S2023, DAKO Corp, Carpinteria, CA, United States) for 20 min, the sections were washed and incubated for 18-20 h at 4 °C with a rabbit polyclonal antibody against NGF (1:700, OriGene Technologies, Inc., Rockville, MD, United States), M2 (1:700, Millipore, Temecula, CA, United States), M3 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, United States), MafA (1:200, Abcam, Cambridge, MA, United States), or PDX-1 (1:100, Abcam), a goat polyclonal antibody against CGRP (1:200, Abcam), or a mouse monoclonal antibody against substance P (1:75, Abcam) or insulin (1:1200, Thermo Fisher Scientific Inc., Waltham, MA, United States).

Techniques: MANN-WHITNEY