mouse monoclonal glyceraldehyde 3 phosphate dehydrogenase gapdh 1 1000  (Millipore)


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    Structured Review

    Millipore mouse monoclonal glyceraldehyde 3 phosphate dehydrogenase gapdh 1 1000
    Mouse Monoclonal Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh 1 1000, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal glyceraldehyde 3 phosphate dehydrogenase gapdh 1 1000/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal glyceraldehyde 3 phosphate dehydrogenase gapdh 1 1000 - by Bioz Stars, 2020-04
    90/100 stars

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    Radio Immunoprecipitation:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: SDS-PAGE, western blotting and quantitation Cells or tissues were washed in PBS and protein extracted in radioimmunoprecipitation assay buffer (RIPA) (20 mM Tris pH 7.4, 150 mM sodium chloride, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 mM EDTA, 0.1% SDS) supplemented with a Complete Mini Protease Inhibitor EDTA free tablet (Roche) and a PhosSTOP phosphatase inhibitor cocktail tablet (Roche). .. Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    Protease Inhibitor:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: SDS-PAGE, western blotting and quantitation Cells or tissues were washed in PBS and protein extracted in radioimmunoprecipitation assay buffer (RIPA) (20 mM Tris pH 7.4, 150 mM sodium chloride, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 mM EDTA, 0.1% SDS) supplemented with a Complete Mini Protease Inhibitor EDTA free tablet (Roche) and a PhosSTOP phosphatase inhibitor cocktail tablet (Roche). .. Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    Quantitation Assay:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: Paragraph title: SDS-PAGE, western blotting and quantitation ... Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    Protein Concentration:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: Protein concentration was estimated using the Direct Detect infrared (IR)-based quantification, according to manufacturer's instructions (Merck Millipore). .. Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    Western Blot:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: Paragraph title: SDS-PAGE, western blotting and quantitation ... Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    SDS Page:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: Paragraph title: SDS-PAGE, western blotting and quantitation ... Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich).

    Staining:

    Article Title: Cell Elasticity Is Regulated by the Tropomyosin Isoform Composition of the Actin Cytoskeleton
    Article Snippet: Blots were probed with the following antibodies: mouse monoclonal γ/9d (1:500) detecting Tpm3.1 and Tpm3.2; rabbit polyclonal δ/9d (1:500) detecting Tpm4.2; rabbit polyclonal α/9c (1:500) detecting Tpm1.10 and Tpm1.12; sheep polyclonal α/9b (1:500) detecting Tpm1.11; mouse monoclonal Tm311 (1:500) (Sigma Aldrich), detecting Tpm1.10, Tpm2.1, Tpm1.6 and Tpm1.7 (all Tpm antibodies are described in [ ]); mouse monoclonal α-tubulin (1:1000) (clone DM 1A, Sigma Aldrich); mouse monoclonal C4 actin (1:1000) [ ]; mouse monoclonal vimentin (1:1000) (Sigma Aldrich) and mouse monoclonal Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) (1:1000) (Sigma Aldrich). .. Protein loading was also estimated following the staining of the blots with Ponceau stain [ ].

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  • 90
    Millipore monoclonal mouse anti glyceraldehyde 3 phosphate dehydrogenase antibody gapdh
    Monoclonal Mouse Anti Glyceraldehyde 3 Phosphate Dehydrogenase Antibody Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti glyceraldehyde 3 phosphate dehydrogenase antibody gapdh/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti glyceraldehyde 3 phosphate dehydrogenase antibody gapdh - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    91
    Millipore anti glyceraldehyde 3 phosphate dehydrogenase mouse monoclonal antibody
    Anti Glyceraldehyde 3 Phosphate Dehydrogenase Mouse Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glyceraldehyde 3 phosphate dehydrogenase mouse monoclonal antibody/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti glyceraldehyde 3 phosphate dehydrogenase mouse monoclonal antibody - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    92
    Millipore anti glyceraldehyde 3 phosphate dehydrogenase gapdh mouse monoclonal antibody
    Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Mouse Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glyceraldehyde 3 phosphate dehydrogenase gapdh mouse monoclonal antibody/product/Millipore
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti glyceraldehyde 3 phosphate dehydrogenase gapdh mouse monoclonal antibody - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

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