Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Treatment with NaHS inhibits the dysregulation of neuroplasticity induced by Quin. (A, B) Immunofluorescence images showing colocalization of PSD95 (red, CoraLite594 fluorescent dye) and NeuN (green, CoraLite488 fluorescent dye) in nine coronal sections from three mice per group. PSD95 expression was significantly upregulated in neuronal cells in the Quin + NaHS group compared with the Quin group, and this effect was reversed by the addition of ML385. Scale bar: 20 μm. (C) Western blot was used to measure PSD95, SYT1, and Syn expression levels 6 days after Quin striatal injection. Quantification of PSD95, SYT1, and Syn expression levels ( n = 3 per group). The values are reported as mean ± SD. *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). NeuN: Neuronal nuclei; PSD95: postsynaptic density protein 95; Quin: quinolinic acid; Syn: synaptophysin; SYT1: synaptotagmin 1.

Article Snippet: Mouse monoclonal anti-NeuN antibody [1B7] - neuronal marker , Abcam (Cambridge, MA, USA) , ab104224 (RRID: AB_10711040).

Techniques: Immunofluorescence, Expressing, Western Blot, Injection

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Reagents and instruments used in the study

Article Snippet: Mouse monoclonal anti-synaptophysin antibody , Proteintech Group (Rosemont, IL, USA) , 60191-1-1g (RRID: AB_10915965).

Techniques: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Treatment with NaHS inhibits the dysregulation of neuroplasticity induced by Quin. (A, B) Immunofluorescence images showing colocalization of PSD95 (red, CoraLite594 fluorescent dye) and NeuN (green, CoraLite488 fluorescent dye) in nine coronal sections from three mice per group. PSD95 expression was significantly upregulated in neuronal cells in the Quin + NaHS group compared with the Quin group, and this effect was reversed by the addition of ML385. Scale bar: 20 μm. (C) Western blot was used to measure PSD95, SYT1, and Syn expression levels 6 days after Quin striatal injection. Quantification of PSD95, SYT1, and Syn expression levels ( n = 3 per group). The values are reported as mean ± SD. *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). NeuN: Neuronal nuclei; PSD95: postsynaptic density protein 95; Quin: quinolinic acid; Syn: synaptophysin; SYT1: synaptotagmin 1.

Article Snippet: Mouse monoclonal anti-synaptophysin antibody , Proteintech Group (Rosemont, IL, USA) , 60191-1-1g (RRID: AB_10915965).

Techniques: Immunofluorescence, Expressing, Western Blot, Injection

Journal: Neural Regeneration Research

Article Title: Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

doi: 10.4103/NRR.NRR-D-24-00130

Figure Lengend Snippet: TCP increases SESN2 levels in NE mice cochleae. (A) Representative images of the medial cochlear turn in the control, NE+saline, and NE+TCP groups ( n = 3 cochleae from three mice per group). Hair cells were visualized by immunofluorescent staining for phalloidin (red), and the tissues were also stained for SESN2 (green), and nuclei (DAPI; blue). (B) Hair cells were visualized by immunofluorescent staining for phalloidin (red), and the tissues were also stained for NLRP3 (green), and nuclei (DAPI; blue) (n=3 cochleae from three mice per group). Scale bars: 20 μm in A and B. (C) Quantification of SESN2- and NLRP3-positive cells among the three groups. (D) SESN2 expression in mice cochleae ( n = 3 cochleae from three mice per group). All experiments were replicated thrice. Data are presented as mean ± standard of the mean. * P < 0.05, ** P < 0.01, **** P < 0.0001 (one-way analysis of variance with Bonferroni’s multiple comparison test). Ctrl: Control; DAPI: 4,6-diamidino-2-phenylindole; NE: noise exposure; ns: not significant; TCP: tranylcypromine.

Article Snippet: First, the membranes were incubated overnight at 4°C with the following primary antibodies: mouse monoclonal anti-SESN2 (1:200, sc-393195, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-NLRP3 (1:400, NBP2-12446, Novus Biologicals, Centennial, CO, USA), rabbit polyclonal anti-LC3B (1:250, ab192890, Abcam, Cambridge, UK), rat monoclonal anti-LAMP1 (1:200, 14-1071-82, Invitrogen, Waltham, MA, USA), and mouse monoclonal 4-hydroxynonenal (4-HNE) (1:250, MA5-27570, Invitrogen).

Techniques: Control, Saline, Staining, Expressing, Comparison