mouse monoclonal anti trf2 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
mouse monoclonal anti trf2
Mouse Monoclonal Anti Trf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti-trf2/TRF2+Antibody/pmc12830668-280-42-48
Average 93 stars, based on 166 article reviews
Mouse Monoclonal Anti Trf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti-trf2/TRF2+Antibody/pmc12830668-280-42-48
Average 93 stars, based on 166 article reviews
mouse monoclonal anti trf2 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress"
Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress
Journal: Communications Biology
doi: 10.1038/s42003-025-09367-z
Figure Legend Snippet: a The diagram shows the workflow on a timeline: on the day after cell seeding, cells were treated with RHPS4 (0.2 and/or 0.5 μM). At 48 h after treatment, cells were again treated with RHPS4 (0.2 and/or 0.5 μM) or harvested and counted. At 72 h after the first treatment, the cell viability was checked, and finally at 96 h after the first treatment, the cells were harvested and processed. b Representative images showing U251MG cells stained by immunofluorescence using anti-BLM (red signals), and anti-TRF1 or anti-TRF2 (green signals) antibodies. Merged images allow visualization of colocalizing dots (yellow signals). Yellow arrows indicate BLM and TRF1-TRF2 colocalizations. c Quantification of the colocalizations between BLM and TRF1 or TRF2 proteins in U251MG cell line upon RHPS4 treatment (0.2 and 0.5 μM). d , e Sensitivity of U251MG and BLM −/− cell lines to RHPS4 concentrations ranging from 0.01 to 2 μM (0.01; 0.125; 0.25; 0.5; 1; 2 μM), evaluated 96 h after the first treatment. Mitomycin C (MMC) was used as a positive control at concentrations of 0.1; 0.5; 1; 2; 5 μg/ml. The Sulforhodamine B (SRB) cytotoxicity assay showed that RHPS4 sensitivity was unchanged in U251MG and BLM −/− (IC50 was 0.56 μM in both cell lines). f Short-term cell proliferation in untreated cells, U251MG and BLM −/− , and in RHPS4-treated cells (0.5 μM) as evaluated 48 and 96 h after the first treatment. g Long-term cell proliferation in U251MG and BLM −/− untreated and RHPS4-treated cells (0.5 μM). Scale bars represent 5 μm. * p < 0.05, ** p < 0.01 (two-way ANOVA; n = 3). Error bars denote the standard deviation of the mean.
Techniques Used: Staining, Immunofluorescence, Positive Control, Cytotoxicity Assay, Standard Deviation
Related Articles
Incubation:Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress Article Snippet: .. The slides were then incubated in blocking buffer (1% BSA dissolved in 1X PBS [w/v]) and then incubated overnight (ON) at 4 °C with the primary antibodies (rabbit polyclonal anti-BLM (#A300-110A, Bethyl) (1:100); mouse monoclonal anti-TRF1 (4E4 clone, GTX70304, GeneTex) (1:20) or Blocking Assay:Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress Article Snippet: .. The slides were then incubated in blocking buffer (1% BSA dissolved in 1X PBS [w/v]) and then incubated overnight (ON) at 4 °C with the primary antibodies (rabbit polyclonal anti-BLM (#A300-110A, Bethyl) (1:100); mouse monoclonal anti-TRF1 (4E4 clone, GTX70304, GeneTex) (1:20) or Western Blot:Article Title: Doxorubicin-Induced Cardiac Senescence Is Alleviated Following Treatment with Combined Polyphenols and Micronutrients through Enhancement in Mitophagy Article Snippet: Densitometric analysis of the bands, relative to housekeeping proteins β-Actin or GAPDH, was determined by ImageJ Software v1.51 (NIH, Bethesda, MD, USA). .. The following primary antibodies were used for Western Blot analysis: mouse monoclonal anti-p21 (sc-6264; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); mouse monoclonal anti-p16 (sc-1661; Santa Cruz Biotechnology Inc., Dallas, TX, USA); rabbit polyclonal anti-phospho-p53 (Ser15) (#9284; Cell Signaling Technology Inc., Danvers, MA, USA); rabbit polyclonal anti-total p53 (sc-6243; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit monoclonal anti-phospho (Ser795) Rb (#9301; Cell Signaling Technology Inc., Danvers, MA, USA); mouse monoclonal anti-Rb (sc-102; Santa Cruz Biotechnology Inc., Dallas, TX, USA); mouse monoclonal anti-TRF1 (NB110-68281, Novus Biologicals, Centennial, CO, USA); Article Title: Doxorubicin-Induced Cardiac Senescence Is Alleviated Following Treatment with Combined Polyphenols and Micronutrients through Enhancement in Mitophagy. Article Snippet: Densitometric analysis of the bands, relative to housekeeping proteins β-Actin or GAPDH, was determined by ImageJ Software v1.51 (NIH, Bethesda, MD, USA). .. The following primary antibodies were used for Western Blot analysis: mouse monoclonal anti-p21 (sc-6264; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); mouse monoclonal anti-p16 (sc-1661; Santa Cruz Biotechnology Inc., Dallas, TX, USA); rabbit polyclonal anti-phospho-p53 (Ser15) (#9284; Cell Signaling Technology Inc., Danvers, MA, USA); rabbit polyclonal anti-total p53 (sc-6243; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit monoclonal anti-phospho (Ser795) Rb (#9301; Cell Signaling Technology Inc., Danvers, MA, USA); mouse monoclonal anti-Rb (sc-102; Santa Cruz Biotechnology Inc., Dallas, TX, Cells 2023, 12, 2605 4 of 23 USA); mouse monoclonal anti-TRF1 (NB110-68281, Novus Biologicals, Centennial, CO, USA); SDS Page:Article Title: Association of a Platinum Complex to a G-Quadruplex Ligand Enhances Telomere Disruption. Article Snippet: Chemical Research in Toxicology is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society. Article Title: Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage. Article Snippet: Western blots were performed following Bio-Rad protocol. .. Briefly, 20 μg proteins were electrophoresed in SDS-PAGE (SDSPolyacrylamide 10%) under denaturing conditions, then transferred to a PVDFmembrane (Polyvinylidin Difluoride) (Amersham HybondTM P+, GE Healthcare) or nitrocellulose, which were hybridized with Membrane:Article Title: Association of a Platinum Complex to a G-Quadruplex Ligand Enhances Telomere Disruption. Article Snippet: Chemical Research in Toxicology is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society. |
