mouse monoclonal anti glast eaat1  (Abcam)

Journal: eLife

Article Title: DRAXIN regulates interhemispheric fissure remodelling to influence the extent of corpus callosum formation

doi: 10.7554/eLife.61618

Figure Lengend Snippet: Schema of interhemispheric midline at E12 ( A ), E15 ( D dorsal; G ventral) and E17 ( I ). In situ hybridisation for Draxin mRNA (white or green), with immunohistochemistry for astroglial marker, GLAST (red), and leptomeninges and IHF marker, Laminin (LAM; magenta) in E12 ( B ), E15 ( E ), and E17 ( J ) wildtype CD1 mid-horizontal telencephalic midline tissue sections. Yellow arrowheads indicate Draxin -positive/GLAST-positive glia. Open red arrowheads indicate lack of Draxin mRNA within the IHF (yellow outlined). Immunohistochemistry for DRAXIN (white or green), GLAST (red or magenta), and LAM (magenta) in E12 ( C ), E15 ( F ), and E17 ( K ) wildtype CD1 mid-horizontal telencephalic midline tissue sections. ( H ) DRAXIN (white or green), axonal marker GAP43 (red), and LAM (magenta) in E15 ventral telencephalic midline tissue sections. Yellow arrowheads indicate regions of DRAXIN protein on GLAST-positive glial fibres ( C, F, K ) or DRAXIN protein on GAP43-positive axons ( H’ ). White arrowheads indicate DRAXIN protein within the IHF and on the basement membrane of the IHF. BM: basement membrane; CCx: cingulate cortex; IGG: indusium griseum glia; LM: leptomeninges; MZGp: midline zipper glia progenitors; Se: septum; Th: telencephalic hinge; 3V: third ventricle.

Article Snippet: Antibody , Mouse monoclonal anti-Glast (EAAT1) , Abcam , Ab49643, RRID: AB_869830 , ‘(1:500)’.

Techniques: In Situ, Hybridization, Immunohistochemistry, Marker

Journal: eLife

Article Title: DRAXIN regulates interhemispheric fissure remodelling to influence the extent of corpus callosum formation

doi: 10.7554/eLife.61618

Figure Lengend Snippet: Wildtype C57 and BTBR pregnant mice were injected with ethynyl deoxyuridine (EdU) every 24 hr from E12 and collected 24 hr later ( D ). Immunohistochemistry for GLAST (white), EdU (green), and cell cycle marker, KI67 (red) on E13 ( A ), E14 ( B ), and E15 ( C ) wildtype C57 and BTBR horizontal brain sections of the telencephalic hinge and interhemispheric fissure (IHF) base. ( E ) To determine whether the litters were age-matched, the total length of the midline was compared between groups. The percentage of EdU-positive/DAPI-positive, EdU-positive/KI67-positive, and EdU-positive/KI67-negative MZG from the telencephalic hinge (white dotted outline) is quantified in ( F ), ( G ), and ( H ), respectively. EdU-positive cells within the base of the IHF is quantified in ( I ). EdU-positive/KI67-positive cells or EdU-positive/KI67-negative cells within the IHF were normalised to the total volume of the IHF as quantified in ( J ) and ( K ), respectively. Data represent mean ± SEM, *p<0.05, **p<0.01, ***p<0.001, ns = not significant, as determined with Mann–Whitney tests. ( L ) Schema of major steps involved in IHF remodelling. ( M ) Schema of BTBR phenotype at E15 compared with wildtype C57: BTBR mice display increased proliferation of MZG progenitors and precocious migration to the IHF surface as well as proliferation of the leptomeninges and expansion of the IHF, which may underlie failed IHF remodelling in these mice. See related . Figure 8—source data 1. Measurements of IHF length and cells expressing EdU or KI67 within the telencephalic midline of E13-E15 BTBR and C57 mice.

Article Snippet: Antibody , Mouse monoclonal anti-Glast (EAAT1) , Abcam , Ab49643, RRID: AB_869830 , ‘(1:500)’.

Techniques: Injection, Immunohistochemistry, Marker, MANN-WHITNEY, Migration, Expressing

Journal: eLife

Article Title: DRAXIN regulates interhemispheric fissure remodelling to influence the extent of corpus callosum formation

doi: 10.7554/eLife.61618

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-Glast (EAAT1) , Abcam , Ab49643, RRID: AB_869830 , ‘(1:500)’.

Techniques: Recombinant, Sequencing, Mutagenesis, Imaging, Software

Journal: Neuron

Article Title: APOE4 causes widespread molecular and cellular alterations associated with Alzheimer’s disease phenotypes in human iPSC-derived brain cell types

doi: 10.1016/j.neuron.2018.05.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-GLAST-PE , Mitenyi Biotec , Cat#130-098-804, RRID: AB_2660782.

Techniques: Transduction, Recombinant, Enzyme-linked Immunosorbent Assay, Cholesterol Assay, Cell Viability Assay, Software

Journal: Molecules and Cells

Article Title: Ependymal Cells Require Anks1a for Their Proper Development

doi: 10.14348/molcells.2018.0432

Figure Lengend Snippet: (A–D) Experiments were performed essentially as described in , with the exception that the GLAST antibody was used in this experiment. Arrowheads represent S100β-positive cells also expressing Glast. Scale bars, 20 μm for panel A and 10 μm for panel C. (E) The data in panels A and B were quantified and presented as mean ± standard deviation (SD). * p < 0.01; ** p < 0.005 (Five adjacent sections for each mouse (n = 5) and littermate (n = 5) were analyzed for comparison). WT versus KO for S100β + cells, unpaired t -test.

Article Snippet: Monoclonal mouse S100β and GFAP antibodies used in this study were purchased from Sigma Aldrich, and the monoclonal mouse glutamate aspartate transporter (GLAST) antibody was purchased from EMD Millipore.

Techniques: Expressing, Standard Deviation