Journal: Current biology : CB
Article Title: p53 Prevents Entry into Mitosis with Uncapped Telomeres
doi: 10.1016/j.cub.2010.01.046
Figure Lengend Snippet: (A) HeLa 1.2.11 cells were arrested in mitosis with colcemid treatment and mitotic chromosomes spread onto glass slides. Preparations were stained with anti-TRF2 (green) and anti-γH2AX antibodies (red). DNA was counterstained with DAPI (blue).
Article Snippet: The following antibodies were used for immunoblotting: rabbit polyclonal antisera raised against human TRF2 [ 31 ], mouse TRF2 (2390 [ 32 ]), human histone H3 (a gift from A. Verreault), Ser15-p53 (Cell Signaling), and human p21 (C-19, Santa Cruz); and mouse monoclonal antibodies raised against phosphorylated ATM-Ser1981 (Cell Signaling), mouse TRF1 (TRF-78, Abcam), p53 (DO-1, Santa Cruz Biotechnology), 14-3-3σ (Upstate), and α-tubulin [ 31 ] (Cancer Research UK Monoclonal Antibody Service).
Techniques: Staining