Journal: Disease Markers

Article Title: ETC-1002 Attenuates Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells via the AMPK/NF- κ B Pathway and Exerts Ameliorative Effects in Experimental Periodontitis in Mice

doi: 10.1155/2022/8583674

Figure Lengend Snippet: ETC-1002 suppresses Pg -LPS-induced inflammation in RAW264.7 cells. (a) Cytotoxic effect of ETC-1002 at different concentrations on RAW264.7 for 24 h or 48 h. (b) qRT-PCR analysis of IL-6, IL-1 β , and TNF- α mRNA expression in Pg -LPS (10 μ g/mL)-treated RAW264.7 cells with or without ETC-1002 (50, 100 μ M) for 12 h. (c) ELISA analysis of IL-6, IL-1 β , and TNF- α protein level in Pg -LPS (10 μ g/mL)-treated RAW264.7 cells with or without ETC-1002 (50, 100 μ M) for 12 h. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: The levels of IL-6, IL-1 β , and TNF- α secreted by the RAW264.7 cells were detected using ELISA kits (Boster Biological Technology, California, USA): mouse IL-1 β ELISA kit (EK0394), mouse TNF- α ELISA kit (EK0527), and mouse IL-6 ELISA kit (EK0411).

Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Disease Markers

Article Title: ETC-1002 Attenuates Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells via the AMPK/NF- κ B Pathway and Exerts Ameliorative Effects in Experimental Periodontitis in Mice

doi: 10.1155/2022/8583674

Figure Lengend Snippet: ETC-1002 ameliorates Pg -LPS-induced inflammation in RAW264.7 cells via AMPK-mediated pathway. (a) Western blot analysis of AMPK α phosphorylation in RAW264.7 cells treated with ETC-1002 (0-200 μ M) for 1 h. (b) mRNA expression of IL-6, IL-1 β , and TNF- α in scramble or AMPK siRNA-transfected RAW264.7 cells treated with Pg -LPS (10 μ g/mL) and ETC-1002 (50, 100 μ M) for 12 h. (c) Western blot analysis of IL-6, IL-1 β , and TNF- α in scramble or AMPK α siRNA-transfected RAW264.7 cells treated with Pg -LPS (10 μ g/mL) and ETC-1002 (50, 100 μ M) for 12 h. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: The levels of IL-6, IL-1 β , and TNF- α secreted by the RAW264.7 cells were detected using ELISA kits (Boster Biological Technology, California, USA): mouse IL-1 β ELISA kit (EK0394), mouse TNF- α ELISA kit (EK0527), and mouse IL-6 ELISA kit (EK0411).

Techniques: Western Blot, Expressing, Transfection

Journal: Disease Markers

Article Title: ETC-1002 Attenuates Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammation in RAW264.7 Cells via the AMPK/NF- κ B Pathway and Exerts Ameliorative Effects in Experimental Periodontitis in Mice

doi: 10.1155/2022/8583674

Figure Lengend Snippet: Anti-inflammation effects of ETC-1002 in periodontitis model in vivo. (a) IHC staining of IL-6, IL-1 β , and TNF- α 10 days after treatment. (b) Relative density of IL-6-, IL-1 β -, and TNF- α -positive area was measured in five fields (400x) located in 1 mm over the alveolar bone crest (between the maxillary first molar and second molar), and the averaged values were calculated. (c) Relative mRNA expression of IL-6, IL-1 β , and TNF- α in the periodontium from the four groups. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

Article Snippet: The levels of IL-6, IL-1 β , and TNF- α secreted by the RAW264.7 cells were detected using ELISA kits (Boster Biological Technology, California, USA): mouse IL-1 β ELISA kit (EK0394), mouse TNF- α ELISA kit (EK0527), and mouse IL-6 ELISA kit (EK0411).

Techniques: In Vivo, Immunohistochemistry, Expressing

Journal: Brain Sciences

Article Title: IL-33 Alleviates Postoperative Cognitive Impairment by Inhibiting Hippocampal Inflammation and Upregulating Excitatory Synaptic Number in Aged Mice

doi: 10.3390/brainsci12091244

Figure Lengend Snippet: IL-33 inhibits surgery/anesthesia-induced hippocampal inflammation in aged mice. Activation of microglia in the hippocampus was indicated by Iba1 ( A , B ). The levels of TNF-α ( C ), IL-1β ( D ) and IL-10 ( E ) were detected by ELISA 3 days after surgery. Data are expressed as the mean ± SD (one-way ANOVA followed by Bonferroni’s post hoc test, n = 4 per group). ** p < 0.01 and *** p < 0.001 versus the control group; # p < 0.05 and ## p < 0.01 versus the surgery group.

Article Snippet: The levels of tumor necrosis factor-alpha (TNF-α) (Boster Biological Technology, Wuhan, China, EK0527), interleukin-1 beta (IL-1β) (Boster, EK0394) and IL-10 (Boster, EK0417) in the hippocampus were detected by an ELISA kit in accordance with the manufacturer’s instructions and represented as pg/mL.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay