mouse anti vegf a monoclonal antibody  (R&D Systems)

 
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    Name:
    Mouse VEGF R3 Flt 4 Antibody
    Description:
    The Mouse VEGF R3 Flt 4 Antibody from R D Systems is a rat monoclonal antibody to VEGF R3 Flt 4 This antibody reacts with mouse The Mouse VEGF R3 Flt 4 Antibody has been validated for the following applications Western Blot
    Catalog Number:
    MAB743-SP
    Price:
    99
    Category:
    Primary Antibodies
    Applications:
    Western Blot
    Purity:
    Protein A or G purified from hybridoma culture supernatant
    Conjugate:
    Unconjugated
    Immunogen:
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4, Tyr25-Asp770, Accession # Q5SU94
    Size:
    25 ug
    Host:
    Rat
    Isotype:
    IgG1
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    Structured Review

    R&D Systems mouse anti vegf a monoclonal antibody
    Immunohistochemical staining for <t>VEGF-A</t> xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.
    The Mouse VEGF R3 Flt 4 Antibody from R D Systems is a rat monoclonal antibody to VEGF R3 Flt 4 This antibody reacts with mouse The Mouse VEGF R3 Flt 4 Antibody has been validated for the following applications Western Blot
    https://www.bioz.com/result/mouse anti vegf a monoclonal antibody/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti vegf a monoclonal antibody - by Bioz Stars, 2021-04
    92/100 stars

    Images

    1) Product Images from "Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios"

    Article Title: Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios

    Journal: American Journal of Cancer Research

    doi:

    Immunohistochemical staining for VEGF-A xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.
    Figure Legend Snippet: Immunohistochemical staining for VEGF-A xxx b, panVEGF and control (mouse IgG) from normal mucosa and from colorectal cancer samples. Staining intensity was scored from 1-4. Scale bar = 100 µm.

    Techniques Used: Immunohistochemistry, Staining

    Predictive staining intensity ratio correlates with plasma VEGF-A xxx b level. A. Correlation of ratio of score of VEGF-A xxx b: panVEGF with plasma VEGF-A xxx b level. r=0.594, P
    Figure Legend Snippet: Predictive staining intensity ratio correlates with plasma VEGF-A xxx b level. A. Correlation of ratio of score of VEGF-A xxx b: panVEGF with plasma VEGF-A xxx b level. r=0.594, P

    Techniques Used: Staining

    VEGF-A xxx b levels measured in plasma. A. Frequency distribution of sample values. B. VEGF-A xxx b levels before and after surgery. BDL= below detection limit. C. Correlation of VEGF-A xxx b levels before and after treatment. R=0.815, P
    Figure Legend Snippet: VEGF-A xxx b levels measured in plasma. A. Frequency distribution of sample values. B. VEGF-A xxx b levels before and after surgery. BDL= below detection limit. C. Correlation of VEGF-A xxx b levels before and after treatment. R=0.815, P

    Techniques Used:

    Related Articles

    Immunohistochemistry:

    Article Title: Vascular endothelial growth factor-D over-expressing tumor cells induce differential effects on uterine vasculature in a mouse model of endometrial cancer
    Article Snippet: Each horn was separated into an upper region (including the ovary, ligature and upper uterine horn; including injection site) and a lower distal region (including the lower uterine horn and cervical junction; away from injection site). .. Immunohistochemistry Antibodies against Lyve-1 (polyclonal goat anti-human Lyve-1, 1 μg/ml; #AF2089, R & D, Minneapolis, MN, USA), podoplanin (goat anti-mouse podoplanin, 1 μg/ml, #AF3244, R & D) and VEGFR-3 (monoclonal rat anti VEGFR-3, 2 μg/ml; clone AFL4, now commercially available from R & D Systems) were used to identify lymphatic vessels. .. Blood vessels were identified using CD31 (rat anti mouse CD31, 5 μg/ml; #553370, BD Pharmingen, Franklin Lakes, NJ, USA).

    Isolation:

    Article Title: Imaging Long-Term Fate of Intramyocardially Implanted Mesenchymal Stem Cells in a Porcine Myocardial Infarction Model
    Article Snippet: .. After about 7 days, when isolated colonies of pig MSCs were apparent, the cells were trypsinized and replated at 8000/cm.2 Immunophenotyping of MSCs was performed by using the following human antibodies conjugated with fluorescin isothiocyanate (FITC) or phycoerythrin (PE): CD44, CD90, pig SLA1, CD31, and CD45 (Becton-Dickinson); VEGFR-3/Flt-4 and CCR7 (R & D Systems); unconjugated LYVE-1 rabbit antibody (AngioBio) was recognized by a secondary AlexaFluor-488-conjugated goat-anti-rabbit antibody (Invitrogen). ..

    other:

    Article Title: Vascular endothelial growth factor secreted by activated stroma enhances angiogenesis and hormone independent growth of estrogen receptor positive breast cancer
    Article Snippet: An anti-mouse VEGF monoclonal antibody (VEGF MAb), the humanized anti-VEGF monoclonal antibody Avastin (Bevacizumab) and AMD 3100, a blocker of the SDF-1 receptor CXCR-4, were tested for their effects on MCF-7 proliferation in BJ3Z co-cultures ( ).

    Article Title: MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1
    Article Snippet: Antibodies and reagentsThe following antibodies and reagents were used: Mouse monoclonal and mouse polyclonal MIEN1 (Abnova; antibody specificity tested and proven in previous studies[ , ]), rabbit polyclonal MIEN1 (Life Technologies; antibody specificity tested in previous studies[ ]), mouse monoclonal GAPDH (Santa Cruz Biotechnology), rabbit monoclonal pNF-κB p65 S536 and rabbit polyclonal MMP-9 (Cell Signaling Technology), mouse monoclonal VEGF and uPA (R & D Systems), mouse monoclonal Alexa Fluor 594 conjugated Phalloidin (Life Technologies), mouse monoclonal E-cadherin (BD Biosciences), Vimentin (supernatant developed in mouse and tested against human antigen, Developmental Studies Hybridoma Bank), anti-mouse and anti-rabbit IgG (Promega), AlexaFluor 488 goat anti-mouse IgG and AlexaFluor 594 goat anti-mouse IgG (Life Technologies) sheep anti-DIG-AP antibody and NBT-BCIP ready-to-use tablets (Roche), sheep serum (Jackson ImmunoResearch), rabbit IgG, BSA, levamisole hydrochloride, Tris-HCl (pH 7.4), nuclease free water, SSC buffer, Xylene, Tween-20, Nuclear Fast Red, Hematoxylin and Eosin (Sigma-Aldrich) and Permount and PBS (Thermo Fisher Scientific).

    Concentration Assay:

    Article Title: Circulating levels of anti-angiogenic VEGF-A isoform (VEGF-Axxxb) in colorectal cancer patients predicts tumour VEGF-A ratios
    Article Snippet: Plasma samples were assessed for expression of both VEGF-Axxx b and panVEGF-A, a measure of both VEGF-Axxx and VEGF-Axxx b isoforms, using Enzyme-Linked Immunosorbent Assay (ELISA). .. An Immunoassay 96 well plate (Thermo Life Sciences) was coated with a mouse anti-VEGF-A165 b monoclonal antibody (Clone 56/1, MAB3045, R & D Systems) at a concentration of 10 µg/ml, covered with parafilm and protected from light, then left shaking at room temperature (RT) overnight (~16 hours). pan VEGF-A capture was mouse anti-VEGF-A monoclonal antibody at 2 µg/ml (MAB293, R & D Systems). ..

    Incubation:

    Article Title: Vascular endothelial growth factor secreted by activated stroma enhances angiogenesis and hormone independent growth of estrogen receptor positive breast cancer
    Article Snippet: Supernatants from control or BJ3Z cells were collected, centrifuged and hybridized to a TranSignal™ mouse angiogenesis antibody array (Panomics, Redwood City CA), as instructed. .. MCF-7/BJ3Z co-cultures were incubated 7 days with anti-mouse VEGF monoclonal antibody (VEGF MAb, R & D systems) or Avastin (Bevacizumab, Genetech Inc. San Francisco, CA) at 0.1μg/ml, or the CXCR-4 antagonist AMD 3100 (Sigma, St Louis, MO) at 5μg/ml, refreshed every 48 hrs. ..

    Article Title: Vascular Endothelial Growth Factor-C Accelerates Diabetic Wound Healing
    Article Snippet: .. Five-μm frozen sections were stained with antibodies to mouse VEGFR-3 (R & D Systems) and the pan-hematopoietic marker CD45 (BD Pharmingen) or monocyte/macrophage marker 2 (MOMA2; Acris Antibodies) followed by incubation with donkey anti-goat Alexa594 and donkey anti-rat Alexa488 secondary antibodies (Molecular Probes). .. VEGF-C was detected using a rabbit antiserum to VEGF-C (no. 6) followed by incubation with Alexa594-conjugated secondary antibodies (Molecular Probes), whereas preimmune serum served as a negative control.

    Staining:

    Article Title: Vascular Endothelial Growth Factor-C Accelerates Diabetic Wound Healing
    Article Snippet: .. Five-μm frozen sections were stained with antibodies to mouse VEGFR-3 (R & D Systems) and the pan-hematopoietic marker CD45 (BD Pharmingen) or monocyte/macrophage marker 2 (MOMA2; Acris Antibodies) followed by incubation with donkey anti-goat Alexa594 and donkey anti-rat Alexa488 secondary antibodies (Molecular Probes). .. VEGF-C was detected using a rabbit antiserum to VEGF-C (no. 6) followed by incubation with Alexa594-conjugated secondary antibodies (Molecular Probes), whereas preimmune serum served as a negative control.

    Marker:

    Article Title: Vascular Endothelial Growth Factor-C Accelerates Diabetic Wound Healing
    Article Snippet: .. Five-μm frozen sections were stained with antibodies to mouse VEGFR-3 (R & D Systems) and the pan-hematopoietic marker CD45 (BD Pharmingen) or monocyte/macrophage marker 2 (MOMA2; Acris Antibodies) followed by incubation with donkey anti-goat Alexa594 and donkey anti-rat Alexa488 secondary antibodies (Molecular Probes). .. VEGF-C was detected using a rabbit antiserum to VEGF-C (no. 6) followed by incubation with Alexa594-conjugated secondary antibodies (Molecular Probes), whereas preimmune serum served as a negative control.

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    R&D Systems vegfr2
    Comparison of VEGF-A-stimulated MSCs and HUVECs The cellular distribution of NRP-1 was examined in MSCs and compared with HUVECs following VEGF-A 165 stimulation. MSCs grown on 0.1% gelatin were cultured for 24 h in serum-free conditions, then co-localization of NRP-1 with either PDGFRα phosphorylated at site Tyr 754 , or PDGFRβ phosphorylated at site Tyr 1021 , was examined by immunofluorescence microscopy. As a comparison, HUVECs grown on 0.1% gelatin were cultured for 4 h in serum-free conditions, then co-localization of NRP-1 with <t>VEGFR2</t> phosphorylated at site Tyr 1175 was similarly determined. ( A ) Control unstimulated HUVEC and ( B ) HUVEC exposed to VEGF-A 165 for 10 min, showing VEGFR2-Tyr 1175 (red) and NRP-1 (green). ( C ) Control unstimulated MSC and ( D ) MSC exposed to VEGF-A 165 for 10 min, showing PDGFRα-Tyr 754 (green) and NRP-1 (red). ( E ) Control unstimulated MSC and ( F ) MSC exposed to 20 ng/ml VEGF-A 165 for 10 min, showing PDGFRβ-Tyr 1021 (red) and NRP-1 (green). Below each image, the corresponding red and green channels which have similar threshold values and the same particle size range are shown, together with their co-localization represented by the image in yellow. The mean number of co-localized particles±S.D. derived from four different single cell images is denoted in yellow. ** P
    Vegfr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegfr2/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    R&D Systems mouse monoclonal anti vegf
    <t>Gal1</t> expression and function as well as the glycophenotype of mouse OIR retinas after <t>anti-VEGF</t> therapy A . Representative Western blot of Gal1 and VEGF from P17 neural retinal extracts of RA and OIR mice injected or not with anti-VEGF mAb. β-actin is shown as a loading control. B . Levels of Gal1 and VEGF were quantified by densitometry and normalized to β-actin. Graph shows results of three independent experiments. C . Gal1 mRNA levels were quantified by qRT-PCR in neurosensory retinas of P17 OIR mice, injected or not with anti-VEGF mAb, and RA (control) conditions. Results were normalized to β-actin and expressed according to the 2 −ΔΔCt method using as calibrator the mRNA level obtained from P17 RA mouse retinas. Data are presented as mean ± SEM or as median and interquartile range according to parametric or not parametric test used for analysis. ns, non-significant, * p
    Mouse Monoclonal Anti Vegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti vegf/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    R&D Systems vegf c
    Immunoarray and ELISA results for (A–D) conditioned media for cells (Cal27, HaCaT, HN12, HN13, HN17, HN30, NOKsi, HNOK26, NOK6TK4, and NOK16TK4) (A) IL-6, (B) IL-8, (C) <t>VEGF,</t> (D) <t>VEGF-C.</t> * levels of individual biomarker were below detection limit
    Vegf C, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf c/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    N/A
    The Human Mouse VEGF B186 Antibody from R D Systems is a mouse monoclonal antibody to VEGF B This antibody reacts with human mouse The Human Mouse VEGF B186 Antibody
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    Image Search Results


    Comparison of VEGF-A-stimulated MSCs and HUVECs The cellular distribution of NRP-1 was examined in MSCs and compared with HUVECs following VEGF-A 165 stimulation. MSCs grown on 0.1% gelatin were cultured for 24 h in serum-free conditions, then co-localization of NRP-1 with either PDGFRα phosphorylated at site Tyr 754 , or PDGFRβ phosphorylated at site Tyr 1021 , was examined by immunofluorescence microscopy. As a comparison, HUVECs grown on 0.1% gelatin were cultured for 4 h in serum-free conditions, then co-localization of NRP-1 with VEGFR2 phosphorylated at site Tyr 1175 was similarly determined. ( A ) Control unstimulated HUVEC and ( B ) HUVEC exposed to VEGF-A 165 for 10 min, showing VEGFR2-Tyr 1175 (red) and NRP-1 (green). ( C ) Control unstimulated MSC and ( D ) MSC exposed to VEGF-A 165 for 10 min, showing PDGFRα-Tyr 754 (green) and NRP-1 (red). ( E ) Control unstimulated MSC and ( F ) MSC exposed to 20 ng/ml VEGF-A 165 for 10 min, showing PDGFRβ-Tyr 1021 (red) and NRP-1 (green). Below each image, the corresponding red and green channels which have similar threshold values and the same particle size range are shown, together with their co-localization represented by the image in yellow. The mean number of co-localized particles±S.D. derived from four different single cell images is denoted in yellow. ** P

    Journal: Biochemical Journal

    Article Title: Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells

    doi: 10.1042/BJ20091512

    Figure Lengend Snippet: Comparison of VEGF-A-stimulated MSCs and HUVECs The cellular distribution of NRP-1 was examined in MSCs and compared with HUVECs following VEGF-A 165 stimulation. MSCs grown on 0.1% gelatin were cultured for 24 h in serum-free conditions, then co-localization of NRP-1 with either PDGFRα phosphorylated at site Tyr 754 , or PDGFRβ phosphorylated at site Tyr 1021 , was examined by immunofluorescence microscopy. As a comparison, HUVECs grown on 0.1% gelatin were cultured for 4 h in serum-free conditions, then co-localization of NRP-1 with VEGFR2 phosphorylated at site Tyr 1175 was similarly determined. ( A ) Control unstimulated HUVEC and ( B ) HUVEC exposed to VEGF-A 165 for 10 min, showing VEGFR2-Tyr 1175 (red) and NRP-1 (green). ( C ) Control unstimulated MSC and ( D ) MSC exposed to VEGF-A 165 for 10 min, showing PDGFRα-Tyr 754 (green) and NRP-1 (red). ( E ) Control unstimulated MSC and ( F ) MSC exposed to 20 ng/ml VEGF-A 165 for 10 min, showing PDGFRβ-Tyr 1021 (red) and NRP-1 (green). Below each image, the corresponding red and green channels which have similar threshold values and the same particle size range are shown, together with their co-localization represented by the image in yellow. The mean number of co-localized particles±S.D. derived from four different single cell images is denoted in yellow. ** P

    Article Snippet: Flow cytometry For single-colour flow cytometry, MSCs (4×106 cells/ml) were incubated with either PE (phycoerythrin)-conjugated anti-human NRP-1 (FAB3870P), VEGFR2 (FAB357P) or control anti-IgG1 (IC002P) (R & D Systems) antibodies, then processed as described previously [ ].

    Techniques: Cell Culture, Immunofluorescence, Microscopy, Derivative Assay

    NRP-1 associated with phosphorylated PDGFRs The association of NRP-1 with PDGFRs was evaluated. ( A ) Flow cytometry analysis of cell surface (i) IgG 1 used as a control, (ii) NRP-1 and (iii) VEGFR2. A representative example of three independent experiments is shown. ( B ) The association of NRP-1 with PDGFRs was examined by immunoprecipitation (IP) followed by immunoblot (IB) analysis. MSCs grown on gelatin and cultured for 24 h in serum-free conditions were unstimulated (Con) or stimulated with either 20 ng/ml PDGF-AA, PDGF-BB or VEGF-A 165 for 10 min at 37 °C, then NRP-1 association with PDGFRs was determined by IP analysis of cell lysates. IP analysis using (i and ii) anti-NRP-1, (iii) anti-PDGFRα or (iv) anti-PDGFRβ, with anti-IgG 1 as a control, then NRP-1 association with PDGFRs detected by IB analysis using (i) anti-PDGFRα, (ii) anti-PDGFRβ or (iii and iv) anti-NRP-1, followed by IB analysis using anti-PDGFRs or NRP-1 as loading controls. A representative of three independent experiments is shown. ( C ) The percentage of (i and ii) total PDGFRs and (iii and iv) phosphorylated PDGFRs interacting with NRP-1 in a particular cell lysate was estimated by IP and IB analysis. Cell lysates were isolated from MSCs which were either unstimulated (Con) (lysates 1 and 3), or exposed to 20 ng/ml PDGF-AA (lysate 2) or PDGF-BB (lysate 4) for 10 min at 37 °C. Each cell lysate was then split into four separate 100 μg aliquots (i–iv) for IP analysis, using either anti-NRP-1, anti-PDGFRα (Rα) or anti-PDGFRβ (Rβ), then IB analysis using (i and ii) anti-PDGFRα or anti-PDGFRβ and (iii and iv) using anti-PDGFRα-Tyr 754 or anti-PDGFRβ-Tyr 1021 . As a loading control blots were re-probed using the corresponding IP antibody. The percentage of total PDGFRα or PDGFRβ interacting with NRP-1 was estimated by quantifying the IB analysis in (i) relative to the corresponding IB analysis in (ii), which was assumed to be 100%. Similarly, the percentage of p-PDGFRα-Tyr 754 or PDGFRβ-Tyr 1021 interacting with NRP-1 was estimated by quantifying the IB analysis in (iii) relative to the corresponding IB analysis in (iv), which was taken to be 100%. This approach gives the proportion of receptor association within the immunoprecipitates, but does not report the total amounts of receptors since the efficiency of each antibody in immunoprecipitating their receptor from a cell lysate may not be 100%. ( D ) Histograms representing the co-immunoprecipitation data, showing the percentage of (i) total PDGFRα or PDGFRβ and (ii) p-PDGFRα-Tyr 754 or PDGFRβ-Tyr 1021 , which interacted with NRP-1. Values are mean percentage values±S.D. determined from two independent experiments. ** P

    Journal: Biochemical Journal

    Article Title: Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells

    doi: 10.1042/BJ20091512

    Figure Lengend Snippet: NRP-1 associated with phosphorylated PDGFRs The association of NRP-1 with PDGFRs was evaluated. ( A ) Flow cytometry analysis of cell surface (i) IgG 1 used as a control, (ii) NRP-1 and (iii) VEGFR2. A representative example of three independent experiments is shown. ( B ) The association of NRP-1 with PDGFRs was examined by immunoprecipitation (IP) followed by immunoblot (IB) analysis. MSCs grown on gelatin and cultured for 24 h in serum-free conditions were unstimulated (Con) or stimulated with either 20 ng/ml PDGF-AA, PDGF-BB or VEGF-A 165 for 10 min at 37 °C, then NRP-1 association with PDGFRs was determined by IP analysis of cell lysates. IP analysis using (i and ii) anti-NRP-1, (iii) anti-PDGFRα or (iv) anti-PDGFRβ, with anti-IgG 1 as a control, then NRP-1 association with PDGFRs detected by IB analysis using (i) anti-PDGFRα, (ii) anti-PDGFRβ or (iii and iv) anti-NRP-1, followed by IB analysis using anti-PDGFRs or NRP-1 as loading controls. A representative of three independent experiments is shown. ( C ) The percentage of (i and ii) total PDGFRs and (iii and iv) phosphorylated PDGFRs interacting with NRP-1 in a particular cell lysate was estimated by IP and IB analysis. Cell lysates were isolated from MSCs which were either unstimulated (Con) (lysates 1 and 3), or exposed to 20 ng/ml PDGF-AA (lysate 2) or PDGF-BB (lysate 4) for 10 min at 37 °C. Each cell lysate was then split into four separate 100 μg aliquots (i–iv) for IP analysis, using either anti-NRP-1, anti-PDGFRα (Rα) or anti-PDGFRβ (Rβ), then IB analysis using (i and ii) anti-PDGFRα or anti-PDGFRβ and (iii and iv) using anti-PDGFRα-Tyr 754 or anti-PDGFRβ-Tyr 1021 . As a loading control blots were re-probed using the corresponding IP antibody. The percentage of total PDGFRα or PDGFRβ interacting with NRP-1 was estimated by quantifying the IB analysis in (i) relative to the corresponding IB analysis in (ii), which was assumed to be 100%. Similarly, the percentage of p-PDGFRα-Tyr 754 or PDGFRβ-Tyr 1021 interacting with NRP-1 was estimated by quantifying the IB analysis in (iii) relative to the corresponding IB analysis in (iv), which was taken to be 100%. This approach gives the proportion of receptor association within the immunoprecipitates, but does not report the total amounts of receptors since the efficiency of each antibody in immunoprecipitating their receptor from a cell lysate may not be 100%. ( D ) Histograms representing the co-immunoprecipitation data, showing the percentage of (i) total PDGFRα or PDGFRβ and (ii) p-PDGFRα-Tyr 754 or PDGFRβ-Tyr 1021 , which interacted with NRP-1. Values are mean percentage values±S.D. determined from two independent experiments. ** P

    Article Snippet: Flow cytometry For single-colour flow cytometry, MSCs (4×106 cells/ml) were incubated with either PE (phycoerythrin)-conjugated anti-human NRP-1 (FAB3870P), VEGFR2 (FAB357P) or control anti-IgG1 (IC002P) (R & D Systems) antibodies, then processed as described previously [ ].

    Techniques: Flow Cytometry, Cytometry, Immunoprecipitation, Cell Culture, Isolation

    Gal1 expression and function as well as the glycophenotype of mouse OIR retinas after anti-VEGF therapy A . Representative Western blot of Gal1 and VEGF from P17 neural retinal extracts of RA and OIR mice injected or not with anti-VEGF mAb. β-actin is shown as a loading control. B . Levels of Gal1 and VEGF were quantified by densitometry and normalized to β-actin. Graph shows results of three independent experiments. C . Gal1 mRNA levels were quantified by qRT-PCR in neurosensory retinas of P17 OIR mice, injected or not with anti-VEGF mAb, and RA (control) conditions. Results were normalized to β-actin and expressed according to the 2 −ΔΔCt method using as calibrator the mRNA level obtained from P17 RA mouse retinas. Data are presented as mean ± SEM or as median and interquartile range according to parametric or not parametric test used for analysis. ns, non-significant, * p

    Journal: Oncotarget

    Article Title: Galectin-1 expression imprints a neurovascular phenotype in proliferative retinopathies and delineates responses to anti-VEGF

    doi: 10.18632/oncotarget.17129

    Figure Lengend Snippet: Gal1 expression and function as well as the glycophenotype of mouse OIR retinas after anti-VEGF therapy A . Representative Western blot of Gal1 and VEGF from P17 neural retinal extracts of RA and OIR mice injected or not with anti-VEGF mAb. β-actin is shown as a loading control. B . Levels of Gal1 and VEGF were quantified by densitometry and normalized to β-actin. Graph shows results of three independent experiments. C . Gal1 mRNA levels were quantified by qRT-PCR in neurosensory retinas of P17 OIR mice, injected or not with anti-VEGF mAb, and RA (control) conditions. Results were normalized to β-actin and expressed according to the 2 −ΔΔCt method using as calibrator the mRNA level obtained from P17 RA mouse retinas. Data are presented as mean ± SEM or as median and interquartile range according to parametric or not parametric test used for analysis. ns, non-significant, * p

    Article Snippet: The following antibodies were used: rabbit polyclonal (1.5μg/ml; anti-Gal1 generated as described [ ]), mouse monoclonal anti-VEGF (1/500; R & D system), rabbit polyclonal anti-GFAP (1/1000; Dako, Carpinteria, CA), mouse monoclonal anti-GS (1/500; Millipore Corporation MA, USA), rabbit polyclonal anti-caspase 3 (Sigma-Aldrich) and mouse monoclonal anti-β-actin (1/2000; Sigma-Aldrich).

    Techniques: Expressing, Western Blot, Mouse Assay, Injection, Quantitative RT-PCR

    Gal1 expression and localization in RA and OIR mouse retinas A . Representative Western blot of Gal1 and VEGF from neural retina extracts of RA and OIR mice at P12, P17 and P26. β-actin is shown as a loading control. B . Levels of Gal1 and VEGF were quantified by densitometry and normalized by β-actin. Results of at least four independent experiments are shown. C . Gal1 mRNA was quantified by qRT-PCR in neurosensory retinas of P17 and P26 OIR mice or RA (control) conditions. Results were normalized to β-actin and expressed according to the 2 −ΔΔCt method using as calibrator the mRNA level obtained from P17 RA mouse retinas. Data are presented as mean ± SEM. ns, non-significant, * p

    Journal: Oncotarget

    Article Title: Galectin-1 expression imprints a neurovascular phenotype in proliferative retinopathies and delineates responses to anti-VEGF

    doi: 10.18632/oncotarget.17129

    Figure Lengend Snippet: Gal1 expression and localization in RA and OIR mouse retinas A . Representative Western blot of Gal1 and VEGF from neural retina extracts of RA and OIR mice at P12, P17 and P26. β-actin is shown as a loading control. B . Levels of Gal1 and VEGF were quantified by densitometry and normalized by β-actin. Results of at least four independent experiments are shown. C . Gal1 mRNA was quantified by qRT-PCR in neurosensory retinas of P17 and P26 OIR mice or RA (control) conditions. Results were normalized to β-actin and expressed according to the 2 −ΔΔCt method using as calibrator the mRNA level obtained from P17 RA mouse retinas. Data are presented as mean ± SEM. ns, non-significant, * p

    Article Snippet: The following antibodies were used: rabbit polyclonal (1.5μg/ml; anti-Gal1 generated as described [ ]), mouse monoclonal anti-VEGF (1/500; R & D system), rabbit polyclonal anti-GFAP (1/1000; Dako, Carpinteria, CA), mouse monoclonal anti-GS (1/500; Millipore Corporation MA, USA), rabbit polyclonal anti-caspase 3 (Sigma-Aldrich) and mouse monoclonal anti-β-actin (1/2000; Sigma-Aldrich).

    Techniques: Expressing, Western Blot, Mouse Assay, Quantitative RT-PCR

    Immunoarray and ELISA results for (A–D) conditioned media for cells (Cal27, HaCaT, HN12, HN13, HN17, HN30, NOKsi, HNOK26, NOK6TK4, and NOK16TK4) (A) IL-6, (B) IL-8, (C) VEGF, (D) VEGF-C. * levels of individual biomarker were below detection limit

    Journal: Analytical chemistry

    Article Title: Ultrasensitive Detection of Cancer Biomarkers in the Clinic using a Nanostructured Microfluidic Array

    doi: 10.1021/ac301392g

    Figure Lengend Snippet: Immunoarray and ELISA results for (A–D) conditioned media for cells (Cal27, HaCaT, HN12, HN13, HN17, HN30, NOKsi, HNOK26, NOK6TK4, and NOK16TK4) (A) IL-6, (B) IL-8, (C) VEGF, (D) VEGF-C. * levels of individual biomarker were below detection limit

    Article Snippet: Antibodies for IL-6 IL-8, VEGF and VEGF-C and protein standards were from R & D systems.

    Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Assay

    Receiver operating characteristic (ROC) curves for serum assays for (A) IL-6, AUC 0.86 ( ), IL-8 with AUC 0.83 ( ), VEGF with AUC 0.95 ( ), VEGF-C with AUC 0.83 ( ), and (B) mean normalized value for all 4 proteins, with AUC 0.96.

    Journal: Analytical chemistry

    Article Title: Ultrasensitive Detection of Cancer Biomarkers in the Clinic using a Nanostructured Microfluidic Array

    doi: 10.1021/ac301392g

    Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for serum assays for (A) IL-6, AUC 0.86 ( ), IL-8 with AUC 0.83 ( ), VEGF with AUC 0.95 ( ), VEGF-C with AUC 0.83 ( ), and (B) mean normalized value for all 4 proteins, with AUC 0.96.

    Article Snippet: Antibodies for IL-6 IL-8, VEGF and VEGF-C and protein standards were from R & D systems.

    Techniques:

    EGCG prevents stimulation of lymphatic endothelial cell migration and tube formation by SUM-149 cells. (A–B) RNA analysis. SUM-149 (left panels) and SUM-190 cells (right panels) were treated with 40 µg/ml EGCG or DMSO (0 µg/ml EGCG) for 72 h. RNA was analyzed by RT-PCR for levels of VEGF-A , VEGF-B , VEGF-C , VEGF-D and GAPDH . (A) Images from a representative experiment are shown. (B) Values for RNA expression from three independent experiments were normalized to the loading control GAPDH and are given as average fold change ± SD relative to the experimental control samples set to 1.0. *, p-value

    Journal: PLoS ONE

    Article Title: Epigallocatechin-3-Gallate Inhibits Stem-Like Inflammatory Breast Cancer Cells

    doi: 10.1371/journal.pone.0073464

    Figure Lengend Snippet: EGCG prevents stimulation of lymphatic endothelial cell migration and tube formation by SUM-149 cells. (A–B) RNA analysis. SUM-149 (left panels) and SUM-190 cells (right panels) were treated with 40 µg/ml EGCG or DMSO (0 µg/ml EGCG) for 72 h. RNA was analyzed by RT-PCR for levels of VEGF-A , VEGF-B , VEGF-C , VEGF-D and GAPDH . (A) Images from a representative experiment are shown. (B) Values for RNA expression from three independent experiments were normalized to the loading control GAPDH and are given as average fold change ± SD relative to the experimental control samples set to 1.0. *, p-value

    Article Snippet: Immunodepleted media were used in Western blot analysis with mouse monoclonal antibodies VEGF-A (MAB293) and VEGF-C (MAB752) [R & D Systems].

    Techniques: Migration, Reverse Transcription Polymerase Chain Reaction, RNA Expression