mouse anti sumo 1 monoclonal antibody (Santa Cruz Biotechnology)
Structured Review

Mouse Anti Sumo 1 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti sumo 1 monoclonal antibody/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
Images
1) Product Images from "CCAAT/Enhancer-Binding Protein ε 27 Antagonism of GATA-1 Transcriptional Activity in the Eosinophil Is Mediated by a Unique N-Terminal Repression Domain, Is Independent of Sumoylation and Does Not Require DNA Binding"
Article Title: CCAAT/Enhancer-Binding Protein ε 27 Antagonism of GATA-1 Transcriptional Activity in the Eosinophil Is Mediated by a Unique N-Terminal Repression Domain, Is Independent of Sumoylation and Does Not Require DNA Binding
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms222312689

Figure Legend Snippet: ( A , B ) C/EBPε is sumoylated in the eosinophil lineage. Detection of endogenous sumoylated C/EBPε in AML14.3D10 eosinophil lysates prepared in the presence of 50 μM N -ethylmaleimide, immunoprecipitated with anti-C/EBPε antibody, and detected by Western blotting using an anti-SUMO-1 antibody ( A ). Detection of sumoylated C/EBPε 32 and C/EBPε 27 in lysates of COS-7 cells transfected as indicated with expression vectors for C/EBPε 27 , C/EBPε 32 , FLAG-SUMO-1, and mutant-FLAG-SUMO-1 (a form of SUMO-1 in which the target glycine has been converted to alanine and is unavailable for conjugation) ( B ). Lysates prepared with 50 μM N -ethylmaleimide were immunoprecipitated with anti-C/EBPε antibody and the Western blot probed with anti-FLAG M2 HRP-conjugated antibody. ( C – E ) Over-expression of SUMO-1 or mutation of the C/EBPε 27 sumoylation site has no effect on the repressor activity of C/EBPε 27 for GATA-1 transactivation of the MBP1-P2 promoter. Co-transfection of a SUMO-1 expression vector with C/EBPε 32 or C/EBPε 27 , does not alter the repressor activity of C/EBPε 27 for GATA-1 trans-activation of the MBP1-P2 promoter, nor convert C/EBPε 32 into a repressor independently or for GATA-1 ( C ) (* p < 0.05, ***, ### p < 0.001, compared to GATA-1 alone, ±SUMO-1, respectively). Mutation of the sumoylation target lysine (K) residue in the conserved SUMO (VKEEP) consensus site to either arginine (K → R) or alanine (K → A) ( D ) does not alter the repressor activity of C/EBPε 27 for GATA-1 transactivation of the MBP1-P2 promoter ( E ) (** p < 0.01; ns , not significant, compared to GATA-1 alone). Mutation of this target lysine in full length C/EBPε 32 ( D ) does not convert it into an activator or repressor in the presence of GATA-1 ( E ).
Techniques Used: Immunoprecipitation, Western Blot, Transfection, Expressing, Mutagenesis, Conjugation Assay, Over Expression, Activity Assay, Cotransfection, Plasmid Preparation, Activation Assay