Journal: Diabetes, Metabolic Syndrome and Obesity
Article Title: PEDF Overexpression Ameliorates Cardiac Lipotoxicity in Diabetic Cardiomyopathy via Regulation of Energy Metabolism
doi: 10.2147/DMSO.S482346
Figure Lengend Snippet: PEDF alleviates cardiac lipotoxicity and regulates cardiac energy metabolism in db/db mice. ( A and B ) Representative Oil O red staining images (×200) and the relevant statistical graph (n=6). ( C ) The TG concentrations of cardiac tissue (n=6). The TG( D ), Glu( E ), TC( F ), HDL-Ch( G ) and LDL-Ch( H ) concentrations in serum were detected (n=6). ( I )The representative Western blot images of ATGL, PPARα, Glut4, CPT1α and CD36, ( J-N ) Quantification of ATGL, PPARα, Glut4, CPT1αand CD36 (n=3). Data are expressed as mean ± SD. ****P<0.0001; **P<0.01; *P<0.05.
Article Snippet: Then, the protein was incubated with the following primary antibodies overnight at 4°C: PEDF rabbit antibody (1:1000, Abcam, ab227295), collagen I rabbit antibody (1:500, Abcam, ab279711), collagen III rabbit antibody (1:1000, Abcam, ab184993), HO-1 rabbit antibody (1:1000, Abcam, ab189491), ATGL rabbit antibody (1:1000, Proteintech, 55190-1-AP), PPARα mouse antibody (1:1000, Proteintech, 66826-1-Ig), CPT1α mouse antibody (1:1000, Proteintech, 66039-1-Ig), Bax rabbit antibody (1:1000, Immunoway, YM8175), Bcl2 rabbit antibody (1:1000, Proteintech,26593-1-AP), Caspase3 rabbit antibody (1:1000, Proteintech, 82202-1-RR), C-caspase3 rabbit antibody (1:1000, Cell Signaling Technology, 9664), Nrf2 rabbit antibody (1:1000, Baijia, IPB6621), CD 36 rabbit antibody (1:1000, Baijia, IPB0697), Glut4 rabbit antibody (1:1000, Baijia, IPB3253), and β-actin mouse antibody (1:3000, Proteintech, 66009-1-Ig).
Techniques: Staining, Western Blot
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