mouse anti pax8  (Abcam)

Journal: Heliyon

Article Title: Prognostic value of PAX8 in small cell lung cancer

doi: 10.1016/j.heliyon.2024.e28251

Figure Lengend Snippet: Expression of PAX8 in clinicopathological characteristics of SCLC.

Article Snippet: The samples were incubated with anti-Mouse PAX8 monoclonal antibody (1:1 dilution; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; clone number: OTI6H8) or anti-Mouse Ki-67 monoclonal antibody (1:2500 dilution; Beijing Zhongshan Golden Bridge Biotechnology; clone number: UMAB107).

Techniques: Expressing

Journal: Heliyon

Article Title: Prognostic value of PAX8 in small cell lung cancer

doi: 10.1016/j.heliyon.2024.e28251

Figure Lengend Snippet: Immunohistochemical expression of PAX8 in SCLC. (A) Extensive-stage SCLC PAX8 positive case. (B) Limited-stage small cell lung cancer PAX8 positive case. (C) Extensive-stage small cell lung cancer PAX8 negative case. (D) limited-stage small cell lung cancer PAX8 negative case.

Article Snippet: The samples were incubated with anti-Mouse PAX8 monoclonal antibody (1:1 dilution; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; clone number: OTI6H8) or anti-Mouse Ki-67 monoclonal antibody (1:2500 dilution; Beijing Zhongshan Golden Bridge Biotechnology; clone number: UMAB107).

Techniques: Immunohistochemical staining, Expressing

Journal: Heliyon

Article Title: Prognostic value of PAX8 in small cell lung cancer

doi: 10.1016/j.heliyon.2024.e28251

Figure Lengend Snippet: Overexpression of PAX8 in HEK-293T. (A) Real-time PCR analysis of PAX8 in HEK-293T. (B) Immunohistochemical expression of PAX8 in HEK-293T.

Article Snippet: The samples were incubated with anti-Mouse PAX8 monoclonal antibody (1:1 dilution; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; clone number: OTI6H8) or anti-Mouse Ki-67 monoclonal antibody (1:2500 dilution; Beijing Zhongshan Golden Bridge Biotechnology; clone number: UMAB107).

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Immunohistochemical staining, Expressing

Journal: Heliyon

Article Title: Prognostic value of PAX8 in small cell lung cancer

doi: 10.1016/j.heliyon.2024.e28251

Figure Lengend Snippet: Comparative survival analyses in SCLC patients. (A) Comparison of OS between PAX8 positive and PAX8 negative group. (B) Comparison of OS between Ki-67 high and Ki-67 low group. (C) Comparison of OS between Limited-stage and Extensive-stage patients. (D) Comparison of OS among PAX8 expression and stage status combination groups. The difference in OS between groups was compared by Log-rank test, with death as the outcome.

Article Snippet: The samples were incubated with anti-Mouse PAX8 monoclonal antibody (1:1 dilution; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; clone number: OTI6H8) or anti-Mouse Ki-67 monoclonal antibody (1:2500 dilution; Beijing Zhongshan Golden Bridge Biotechnology; clone number: UMAB107).

Techniques: Comparison, Expressing

Journal: Heliyon

Article Title: Prognostic value of PAX8 in small cell lung cancer

doi: 10.1016/j.heliyon.2024.e28251

Figure Lengend Snippet: The HR value of Ki-67, PAX8 and stage status for overall survival.

Article Snippet: The samples were incubated with anti-Mouse PAX8 monoclonal antibody (1:1 dilution; Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; clone number: OTI6H8) or anti-Mouse Ki-67 monoclonal antibody (1:2500 dilution; Beijing Zhongshan Golden Bridge Biotechnology; clone number: UMAB107).

Techniques:

Journal: BMC Cancer

Article Title: Claudin18.2 as a potential therapeutic target for primary ovarian mucinous carcinomas and metastatic ovarian mucinous carcinomas from upper gastrointestinal primary tumours

doi: 10.1186/s12885-023-10533-x

Figure Lengend Snippet: Pathological phenotypic features of POMC and MGMC from the upper and lower gastrointestinal tract. P values indicating statistical significance are denoted. p 1: POMC vs. lower gastrointestinal MGMC; p 2: POMC vs. upper gastrointestinal MGMC; p 3: upper vs. lower gastrointestinal MGMC; NS, no significant difference

Article Snippet: The primary antibodies were rabbit anti-claudin18.2 (clone ab222512, Abcam, United Kingdom, 1:500 dilution), rabbit anti-SATB2 (clone ab92446, Abcam, United Kingdom, 1:150 dilution), mouse anti-PAX8 (clone ab53490, Abcam, United Kingdom, 1:100 dilution), mouse anti-CK7 (clone ab9021, Abcam, United Kingdom, 1:500 dilution) and rabbit anti-CK20 (clone ab76126, Abcam, United Kingdom, 1:200 dilution).

Techniques:

Journal: BMC Cancer

Article Title: Claudin18.2 as a potential therapeutic target for primary ovarian mucinous carcinomas and metastatic ovarian mucinous carcinomas from upper gastrointestinal primary tumours

doi: 10.1186/s12885-023-10533-x

Figure Lengend Snippet: HE staining and staining for different immunophenotypic markers in POMCs and MGMCs from the upper and lower gastrointestinal tracts. The immunophenotypic characteristics of POMCs, upper gastrointestinal tract MGMCs, and lower gastrointestinal tract MGMCs were claudin 18.2 + /PAX8 + /SATB2-, claudin 18.2 + /PAX8-/SATB2- and claudin 18.2-/PAX8-/SATB2 + , respectively. (bar = 100 µm)

Article Snippet: The primary antibodies were rabbit anti-claudin18.2 (clone ab222512, Abcam, United Kingdom, 1:500 dilution), rabbit anti-SATB2 (clone ab92446, Abcam, United Kingdom, 1:150 dilution), mouse anti-PAX8 (clone ab53490, Abcam, United Kingdom, 1:100 dilution), mouse anti-CK7 (clone ab9021, Abcam, United Kingdom, 1:500 dilution) and rabbit anti-CK20 (clone ab76126, Abcam, United Kingdom, 1:200 dilution).

Techniques: Staining

Journal: Nature Communications

Article Title: SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse

doi: 10.1038/s41467-022-35465-1

Figure Lengend Snippet: a , b Schematic illustration of the experiment and Uniform Manifold Approximation and Projection (UMAP) plot of cells isolated from the proximal part of the testis of P14 Sox17- cKO and controls, showing twenty clusters ( a ) and the distribution of cells from each sample ( b ). Control n = 2, Sox17- cKO n = 2. c UMAP plot showing the expression level of Sox9, Pax8, and Sox17 in the control samples. The right two panels show the expression of each gene in the extracted clusters of the RT (cluster 16) and Sertoli cells (clusters 1, 2, 10, 18). d Dot plots representing the expression levels of marker genes for Sertoli cells ( Amh ) and RT epithelial cells ( Krt8, Pax8 ) together with their common marker genes ( Nr5a1, Gata4, Wt1, Sox9 ) in the clusters expressing Sox9 shown in ( c ) (clusters 1, 2, 10, 16, 18). e A violin plot showing the expression level of Sox17 in each cluster, together with Pecam1 (vascular endothelial cell marker) and Pax8 (RT marker). “ n ” represents the number of biological replicates.

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies [format: host anti-protein (company, catalog number, dilution)]: mouse monoclonal anti-ace-TUB (Sigma, T6793, 1:200), mouse monoclonal anti-αSMA/ACTA2 (Sigma, A5228, 1:500), goat polyclonal anti-AMH (Santa Cruz, sc6886, 1:200), mouse monoclonal anti-CDH1 (BD Transduction lab, 610181, 1:400), goad polyclonal anti-c-KIT (R&D systems, AF1356, 1:200), rabbit monoclonal anti-EPCAM (Abcam, ab32392, 1:100), goat polyclonal anti-GATA4 (Santa Cruz, sc1237, 1:200), mouse monoclonal anti-GATA4 (Santa Cruz, sc25310, 1:100), rabbit polyclonal anti-GFP (MBL, 598, 1:200), goat polyclonal GFRα1 (R&D systems, AF560, 1:100), rabbit polyclonal anti-HSP70 (Abcam, ab79852, 1:1000), rabbit polyclonal anti-KI67 (Abcam, ab15580, 1:400), rabbit monoclonal anti-KRT8 (Abcam, ab53280, 1:200), rabbit polyclonal LAMININ (Abcam, ab11575, 1:200), rabbit monoclonal anti-p-AKT (Cell Signaling, 4060, 1:100), mouse monoclonal anti-PAX8 (Abcam, ab53490, 1:100), rat monoclonal PECAM1 (Invitrogen, 14-0311-82, 1:100), rabbit polyclonal anti-PLZF (Santa Cruz, sc22839, 1:200), mouse monoclonal SCP3 (Santa Cruz, sc74569, 1:500), goat polyclonal anti-SOX17 (R&D systems, AF1924, 1:200), rabbit polyclonal anti-SOX9 (Millipore, AB5535, 1:400), rabbit polyclonal anti-STAR (Cell Signaling, 8449, 1:100), rabbit polyclonal anti-VASA/MVH (Abcam, ab13840, 1:10000).

Techniques: Isolation, Expressing, Marker

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Gene-gene interaction results for susceptibility to classic Papillary Thyroid Carcinoma.

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: Relative frequencies of the nine genotype combinations of the replicated interaction ( PAX8 - STK17B ) are shown for cases and controls (red and blue columns, respectively). The cell containing the high-risk genotype combination is highlighted in light red, those with low-risk combinations in light blue, and those with neutral combinations are uncoloured. Figure1a - based on the discovery stage (series I); - based on the replication stage (series II and III); – based on both stages combined (series I, II and III).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques:

Journal: PLoS ONE

Article Title: An Epistatic Interaction between the PAX8 and STK17B Genes in Papillary Thyroid Cancer Susceptibility

doi: 10.1371/journal.pone.0074765

Figure Lengend Snippet: PCCl3 cells were transiently (A) or stably (B) silenced for the transcription factor Pax8 (siPax8). As a control, wild type or siScramble transfected cells were used. The expression levels were assessed by means of qRT-PCR (A, upper panel) or western blot (A, lower panel, and B).

Article Snippet: Validation of Pax8 silencing and STK17B expression was tested either by RT-PCR or by Western blot using a polyclonal Pax8 mouse antibody (Biopat, Milan, Italy) or a human STK17B antibody (RD System, Minneapolis, MN), respectively. βactin levels were used as loading control after immunoblotting with a specific antibody (Santa Cruz Biotechnology, CA).

Techniques: Stable Transfection, Transfection, Expressing, Quantitative RT-PCR, Western Blot