mouse anti s100a4 antibody  (Proteintech)

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Effect of DSF on S100A4 + macrophages in the lung tissues of BLM-induced lung fibrosis in mice. ( A – C ) Representative immunostaining images for S100A4 in the lung tissue of untreated ( A ), DSF non-treated ( B ) and DSF-treated ( C ) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. ( D ) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4 + CD68 + macrophages. Scale bars = 25 μm. ( E ) Number of S100A4 + cells in the lung tissue on day 28 counted in immunostaining images ( A – C ). n = 5 per group. * P < 0.05, ** P < 0.01 (Tukey’s test). ns, not significant.

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Immunostaining, Fluorescence, Staining, Generated, Software

Journal: Scientific Reports

Article Title: Antifibrotic effect of disulfiram on bleomycin-induced lung fibrosis in mice and its impact on macrophage infiltration

doi: 10.1038/s41598-024-71770-z

Figure Lengend Snippet: Analysis of multi-fluorescent staining of lung tissues of IPF. ( A , B ) Representative immunofluorescent staining images in normal lung ( A ) and in fibrotic areas of the lungs of IPF patients ( B ). DAPI is shown in pink, α-SMA in brown, CD34 in light blue, CD68 in green, S100A4 in red, and FROUNT in blue. Higher-magnification images on the right correspond to the area indicated with dashed squares. White arrows indicate CD68 + macrophages in normal lung. White arrowheads indicate S100A4 + CD68 + macrophages and yellow arrowheads indicate S100A4 - CD68 + macrophages in fibrotic areas of the lungs of IPF patients. Scale bars = 25 μm. ( C ) S100A4 signals of each CD68 + macrophage in normal lung tissues and in fibrotic areas of the lungs of IPF patients. ( D ) FROUNT signals in S100A4 - CD68 + macrophages and S100A4 + CD68 + macrophages in fibrotic areas of the lungs of IPF patients. n = 5 or 6 per group. ** P < 0.01 (unpaired t-test).

Article Snippet: We used the following primary antibodies: anti-human α-smooth muscle actin (α-SMA) mouse monoclonal antibody (1:250 dilution, Dako, Glostrup, Denmark), anti-human CD34 mouse monoclonal antibody (1:100 dilution, Nichirei Bioscience, Tokyo, Japan), anti-human CD68 mouse monoclonal antibody (1:200 dilution, Dako, Glostrup, Denmark), anti-human/mouse S100A4 rabbit monoclonal antibody (1:100 dilution, Abcam, Cambridge, UK), and anti-human FROUNT goat polyclonal antibody (1:150 dilution, Everest biotech, Oxfordshire, UK), followed by peroxidase-labelled secondary antibodies, and incubated with Opal fluorophore Opal 690, Opal 540, Opal 570, Opal 620, Opal 520, respectively.

Techniques: Staining

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: Increased expression of psoriasin (S100A7) and interleukin 17 (IL-17) in lesional skin in lichen planopilaris

doi: 10.5114/ada.2024.142179

Figure Lengend Snippet: The quantitative evaluation of S100A7, S100A4, IL-17 expression in LPP lesional skin, AA lesional skin and normal skin of healthy controls (values expressed as the mean number of positively stained cells ± SD)

Article Snippet: The immunohistochemical reactions was performed on 4 μm-formalin-fixed and paraffin-embedded (FFPE) biopsy specimens using mouse anti-human psoriasin (S100A7) monoclonal antibodies (catalogue number ab13680; Abcam, UK), mouse anti-human S100A4 monoclonal antibody (catalogue number ab218512; Abcam, UK), mouse anti-human IL-17 polyclonal antibodies (catalogue number PA1-84183; Invitrogen, USA) according to the manufacturer’s protocol, with positive and negative control stainings.

Techniques: Expressing, Staining

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: Increased expression of psoriasin (S100A7) and interleukin 17 (IL-17) in lesional skin in lichen planopilaris

doi: 10.5114/ada.2024.142179

Figure Lengend Snippet: Representative immunohistochemical stainings of S100A4 in LPP lesional skin ( A ), AA lesional skin ( B ), normal skin ( C )

Article Snippet: The immunohistochemical reactions was performed on 4 μm-formalin-fixed and paraffin-embedded (FFPE) biopsy specimens using mouse anti-human psoriasin (S100A7) monoclonal antibodies (catalogue number ab13680; Abcam, UK), mouse anti-human S100A4 monoclonal antibody (catalogue number ab218512; Abcam, UK), mouse anti-human IL-17 polyclonal antibodies (catalogue number PA1-84183; Invitrogen, USA) according to the manufacturer’s protocol, with positive and negative control stainings.

Techniques: Immunohistochemical staining

Journal: Cureus

Article Title: Correlation of S100A4 and S100A14 Expression With Clinico-Pathological Features and Tumor Location in Colorectal Cancer Patients

doi: 10.7759/cureus.65615

Figure Lengend Snippet: Association between S100A4 expression and clinico-pathological features of CRC patients CRC - colorectal cancer

Article Snippet: Immuhistochemistry (IHC) Deparaffinized tissue slides were treated with mouse monoclonal primary antibodies against S100A4 and S100A14 proteins (Santa Cruz, USA).

Techniques: Expressing