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Santa Cruz Biotechnology mouse anti kelch like ech associated protein 1
Mouse Anti Kelch Like Ech Associated Protein 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti kelch like ech associated protein 1/product/Santa Cruz Biotechnology
Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mouse anti kelch like ech associated protein 1 - by Bioz Stars, 2020-04
95/100 stars

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Isolation:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. To investigate nuclear translocation of Nrf2 and cytosolic expression of NQO-1, both nucleus and cytosol proteins isolated from SNpc and striatum were used.

Dissection:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Western Blot Assays At 7 days following the last MPTP intoxication, coronal brain slices (3 mm in thickness) from each mouse (n = 3 per group) were prepared as previous described (Choi et al., ) and SNpc and striatum regions were sampled using microscissors and blade under a dissection microscope. .. Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C.

Microscopy:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Western Blot Assays At 7 days following the last MPTP intoxication, coronal brain slices (3 mm in thickness) from each mouse (n = 3 per group) were prepared as previous described (Choi et al., ) and SNpc and striatum regions were sampled using microscissors and blade under a dissection microscope. .. Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C.

Incubation:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. PVDF membranes were then incubated with horseradish peroxidase-conjugated secondary antibody to enhance chemiluminescence analysis (Amersham Pharmacia Biotech, Piscataway, NJ, USA) and visualized using a super cooled-CCD camera system with a Davinch-K Gel imaging system (Dvinch-K, Seoul, Republic of Korea).

Imaging:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. PVDF membranes were then incubated with horseradish peroxidase-conjugated secondary antibody to enhance chemiluminescence analysis (Amersham Pharmacia Biotech, Piscataway, NJ, USA) and visualized using a super cooled-CCD camera system with a Davinch-K Gel imaging system (Dvinch-K, Seoul, Republic of Korea).

Expressing:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. To investigate nuclear translocation of Nrf2 and cytosolic expression of NQO-1, both nucleus and cytosol proteins isolated from SNpc and striatum were used.

Western Blot:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Paragraph title: Western Blot Assays ... Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C.

Binding Assay:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: .. Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. To investigate nuclear translocation of Nrf2 and cytosolic expression of NQO-1, both nucleus and cytosol proteins isolated from SNpc and striatum were used.

Translocation Assay:

Article Title: Neuroprotective Effects of a Traditional Multi-Herbal Medicine Kyung-Ok-Ko in an Animal Model of Parkinson's Disease: Inhibition of MAPKs and NF-κB Pathways and Activation of Keap1-Nrf2 Pathway
Article Snippet: Briefly, the polyvinylidene fluoride (PVDF) membrane strips with a specific protein from SNpc and striatum were probed overnight with rabbit anti-tyrosine hydroxylase (1:1,000; Millipore, Bedford, MA, USA), rabbit anti-ionized calcium binding adapter molecule-1 (Iba-1; 1:500; WAKO, Osaka, Japan), mouse anti-glial fibrillary acidic protein (GFAP; 1:1,000; Millipore), rat anti-platelet endothelial cell adhesion molecule-1 [PECAM-1 (CD31); 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA], rabbit anti-phospho (p)-extracellular signal-regulated kinase (ERK), rabbit anti-p-c-Jun N-terminal kinase (JNK), rabbit anti-p-p38, rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, rabbit anti-p-IκBα (1:1,000; Cell Signaling Technology, Beverly, MA, USA), mouse anti-Kelch-like ECH-associated protein 1 (Keap1; 1:1,000, Santa Cruz Biotechnology), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000, Santa Cruz Biotechnology), mouse anti-heme oxygenase-1 (HO-1; 1:1,000; Enzo Life Sciences, Farmingdale, NY, USA), or mouse anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1; 1:1,000; Cell Signaling Technology) at 4°C. .. To investigate nuclear translocation of Nrf2 and cytosolic expression of NQO-1, both nucleus and cytosol proteins isolated from SNpc and striatum were used.

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    Santa Cruz Biotechnology mouse anti kelch like ech associated protein 1
    Mouse Anti Kelch Like Ech Associated Protein 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti kelch like ech associated protein 1/product/Santa Cruz Biotechnology
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mouse anti kelch like ech associated protein 1 - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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