mouse anti human gapdh  (Boster Bio)


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    Structured Review

    Boster Bio mouse anti human gapdh
    ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream <t>phospho-Erk1/2.</t> Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to <t>GAPDH</t> as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p
    Mouse Anti Human Gapdh, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human gapdh/product/Boster Bio
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse anti human gapdh - by Bioz Stars, 2022-08
    96/100 stars

    Images

    1) Product Images from "Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function"

    Article Title: Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0052404

    ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream phospho-Erk1/2. Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to GAPDH as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p
    Figure Legend Snippet: ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream phospho-Erk1/2. Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to GAPDH as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p

    Techniques Used: Cell Culture, Incubation, Positive Control, Negative Control

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    Boster Bio gapdh mouse monoclonal antibody
    JMJD3 epigenetically regulates inflammatory cytokines expression. A , LPS-stimulated MECs were analyzed by Western blotting using <t>antibodies</t> specific to JMJD3 and H3K27me3. <t>GAPDH</t> was used as a loading control. The Western blotting data were quantified by densitometry and were normalized to GAPDH. B , MECs were collected and DNA was extracted for ChIP analysis. The level of H3K27me3 on the inflammatory cytokine promoters was measured after GSK-J1 administration in MECs with LPS treatment using the indicated antibodies. Values represent mean ± SEM ( n = 6). ∗ p
    Gapdh Mouse Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gapdh mouse monoclonal antibody/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gapdh mouse monoclonal antibody - by Bioz Stars, 2022-08
    94/100 stars
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    96
    Boster Bio mouse anti human gapdh
    ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream <t>phospho-Erk1/2.</t> Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to <t>GAPDH</t> as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p
    Mouse Anti Human Gapdh, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human gapdh/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human gapdh - by Bioz Stars, 2022-08
    96/100 stars
      Buy from Supplier

    94
    Boster Bio mouse anti bax
    The implantation of 125 I seeds induced the mitochondria-dependent apoptotic pathway compared with the control. A: Western Blotting analysis of <t>Bax,</t> <t>Bcl-2,</t> pro-Caspase-3, Caspase-3, pro-Caspase-8, cleaved-Caspase-8, PARP, P53, β-Actin expression.
    Mouse Anti Bax, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti bax/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti bax - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    JMJD3 epigenetically regulates inflammatory cytokines expression. A , LPS-stimulated MECs were analyzed by Western blotting using antibodies specific to JMJD3 and H3K27me3. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and were normalized to GAPDH. B , MECs were collected and DNA was extracted for ChIP analysis. The level of H3K27me3 on the inflammatory cytokine promoters was measured after GSK-J1 administration in MECs with LPS treatment using the indicated antibodies. Values represent mean ± SEM ( n = 6). ∗ p

    Journal: The Journal of Biological Chemistry

    Article Title: The JMJD3 histone demethylase inhibitor GSK-J1 ameliorates lipopolysaccharide-induced inflammation in a mastitis model

    doi: 10.1016/j.jbc.2022.102017

    Figure Lengend Snippet: JMJD3 epigenetically regulates inflammatory cytokines expression. A , LPS-stimulated MECs were analyzed by Western blotting using antibodies specific to JMJD3 and H3K27me3. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and were normalized to GAPDH. B , MECs were collected and DNA was extracted for ChIP analysis. The level of H3K27me3 on the inflammatory cytokine promoters was measured after GSK-J1 administration in MECs with LPS treatment using the indicated antibodies. Values represent mean ± SEM ( n = 6). ∗ p

    Article Snippet: The GAPDH mouse monoclonal antibody (BM3876, 1:1000 dilution for Western blot analysis) was obtained from Boster Biological Technology Co, Ltd. SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (#9003s) was obtained from Cell Signaling Technology.

    Techniques: Expressing, Western Blot, Chromatin Immunoprecipitation

    Expression of JMJD3 is positively associated with the inflammatory response of LPS-induced mastitis. A – C , gene and protein expression of JMJD3 was detected in untreated and LPS-induced mice using qRT-PCR and Western blotting. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and were normalized to GAPDH. D and E , total protein extracts from mammary glands treated with GSK-J1 were used to detect JMJD3 by Western blotting. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and normalized to GAPDH. Values represent mean ± SEM ( n = 6). ∗ p

    Journal: The Journal of Biological Chemistry

    Article Title: The JMJD3 histone demethylase inhibitor GSK-J1 ameliorates lipopolysaccharide-induced inflammation in a mastitis model

    doi: 10.1016/j.jbc.2022.102017

    Figure Lengend Snippet: Expression of JMJD3 is positively associated with the inflammatory response of LPS-induced mastitis. A – C , gene and protein expression of JMJD3 was detected in untreated and LPS-induced mice using qRT-PCR and Western blotting. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and were normalized to GAPDH. D and E , total protein extracts from mammary glands treated with GSK-J1 were used to detect JMJD3 by Western blotting. GAPDH was used as a loading control. The Western blotting data were quantified by densitometry and normalized to GAPDH. Values represent mean ± SEM ( n = 6). ∗ p

    Article Snippet: The GAPDH mouse monoclonal antibody (BM3876, 1:1000 dilution for Western blot analysis) was obtained from Boster Biological Technology Co, Ltd. SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (#9003s) was obtained from Cell Signaling Technology.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Western Blot

    Tlr4 engages JMJD3-mediated inflammation in MECs. A , the level of H3K27me3 on Tlr4 promoters after GSK-J1 administration in MECs with LPS treatment was measured using ChIP assay. B and C , expressions of Tlr4 after JMJD3 inhibition or knockdown in MECs were analyzed by qRT-PCR. D , LPS-stimulated MECs were analyzed by Western blotting using antibodies specific to Tlr4, phosphor-NF-κB p65, phosphor-IκBα, p65, and IκBα. GAPDH was used as a loading control. E – G , the Western blotting data were quantified by densitometry and were normalized to GAPDH. Values represent mean ± SEM ( n = 6). ∗ p

    Journal: The Journal of Biological Chemistry

    Article Title: The JMJD3 histone demethylase inhibitor GSK-J1 ameliorates lipopolysaccharide-induced inflammation in a mastitis model

    doi: 10.1016/j.jbc.2022.102017

    Figure Lengend Snippet: Tlr4 engages JMJD3-mediated inflammation in MECs. A , the level of H3K27me3 on Tlr4 promoters after GSK-J1 administration in MECs with LPS treatment was measured using ChIP assay. B and C , expressions of Tlr4 after JMJD3 inhibition or knockdown in MECs were analyzed by qRT-PCR. D , LPS-stimulated MECs were analyzed by Western blotting using antibodies specific to Tlr4, phosphor-NF-κB p65, phosphor-IκBα, p65, and IκBα. GAPDH was used as a loading control. E – G , the Western blotting data were quantified by densitometry and were normalized to GAPDH. Values represent mean ± SEM ( n = 6). ∗ p

    Article Snippet: The GAPDH mouse monoclonal antibody (BM3876, 1:1000 dilution for Western blot analysis) was obtained from Boster Biological Technology Co, Ltd. SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (#9003s) was obtained from Cell Signaling Technology.

    Techniques: Chromatin Immunoprecipitation, Inhibition, Quantitative RT-PCR, Western Blot

    ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream phospho-Erk1/2. Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to GAPDH as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p

    Journal: PLoS ONE

    Article Title: Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

    doi: 10.1371/journal.pone.0052404

    Figure Lengend Snippet: ScFvs against Ig1–4 reduce, while scFvs against Fn1–3 enhance levels of phospho-src and downstream phospho-Erk1/2. Cultured SK-N-SH cells were incubated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 for 30 min in serum-free medium. ( A ) Levels of src and phospho-src of cells treated with L1/ecd (at 16.5 µM, right) or untreated (control, left), with lanes 1, 2 and 3 showing representative blots from three independent experiments with L1/ecd treated or untreated cells. ( B ) Src and phospho-src levels of cells treated with non-immune human IgG, L1/ecd or I27 (all at 16.5 µM) or left untreated (control). ( C ) Src and phospho-srclevels of cells treated with the indicated concentrations of scFv I4 or I6. ( D ) Src and phospho-src levels of cells treated with the indicated concentrations of scFv I13 or I27. ( E–F ) Comparison of levels of src and phospho-src ( E ) and Erk1/2 and phospho-Erk1/2 ( F ) in untreated cells (control) or cells treated with IgG, scFv I4, I6, I13 or I27 (all at 16.5 µM). Representative images from three independent experiments are shown in A–E . For the quantification shown in C–F , values of phospho-src ( C–E ) and phospho-Erk1/2 ( F ) were normalized to GAPDH as loading control. Mean values ± SEM from three independent experiments are shown. Asterisks denote significant differences from control. ** p

    Article Snippet: Twenty five µg protein was subjected to 12% SDS-PAGE, transferred onto a PVDF membrane (Millipore, Temecula, CA, USA), and probed with rabbit anti-human src (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-phospho-src (Santa Cruz Biotechnology), mouse anti-phospho-Erk1/2 (Beyotime), rabbit anti-human Erk1/2 (Beyotime), rabbit anti-human Bcl-2 (Biosis, Bei Jing, China), mouse anti-human Bax, mouse anti-human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (BOSTER, Wu Han, China).

    Techniques: Cell Culture, Incubation, Positive Control, Negative Control

    The effect of EPO on the expression of PEPCK and G6Pase in the liver. The relative levels of PEPCK and G6Pase in the livers of individual mice were determined by quantitative RT-PCR and Western blot assays. Data are representative images or expressed as the mean ± SEM of individual group (n = 8 per group) of mice from three separate experiments. ( A ) RT-PCR analysis of the relative levels of PEPCK and G6Pase mRNA transcripts to control GAPDH in the liver. (B) Western blot and quantitative analysis of the relative levels of PEPCK in the liver. ( C ) Western blot and quantitative analysis of the relative levels of G6Pase in the liver. # P

    Journal: PLoS ONE

    Article Title: Erythropoietin Inhibits Gluconeogenesis and Inflammation in the Liver and Improves Glucose Intolerance in High-Fat Diet-Fed Mice

    doi: 10.1371/journal.pone.0053557

    Figure Lengend Snippet: The effect of EPO on the expression of PEPCK and G6Pase in the liver. The relative levels of PEPCK and G6Pase in the livers of individual mice were determined by quantitative RT-PCR and Western blot assays. Data are representative images or expressed as the mean ± SEM of individual group (n = 8 per group) of mice from three separate experiments. ( A ) RT-PCR analysis of the relative levels of PEPCK and G6Pase mRNA transcripts to control GAPDH in the liver. (B) Western blot and quantitative analysis of the relative levels of PEPCK in the liver. ( C ) Western blot and quantitative analysis of the relative levels of G6Pase in the liver. # P

    Article Snippet: The membranes were blocked with 5% fat-free milk and incubated with anti-IR, anti-IRS1 (Millipore, Billerica, USA), anti-JNK, anti-p-JNK (Thr183/Tyr185), anti-p-IRS1 (Ser307), anti-p-IR (Tyr1346), anti-Akt, anti-p-Akt (Ser473), anti-p38, anti-p-p38 (Thr180/Tyr182), anti-TLR4, anti-PEPCK, anti-G6Pase (Cell Signaling Technology, Danvers, USA), anti-GAPDH, anti-tubulin (Boster, Wuhan, China), respectively.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, Western Blot, Reverse Transcription Polymerase Chain Reaction

    The implantation of 125 I seeds induced the mitochondria-dependent apoptotic pathway compared with the control. A: Western Blotting analysis of Bax, Bcl-2, pro-Caspase-3, Caspase-3, pro-Caspase-8, cleaved-Caspase-8, PARP, P53, β-Actin expression.

    Journal: American Journal of Translational Research

    Article Title: Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model

    doi:

    Figure Lengend Snippet: The implantation of 125 I seeds induced the mitochondria-dependent apoptotic pathway compared with the control. A: Western Blotting analysis of Bax, Bcl-2, pro-Caspase-3, Caspase-3, pro-Caspase-8, cleaved-Caspase-8, PARP, P53, β-Actin expression.

    Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membrane and immune complexes were formed by incubation of the proteins probed with primary antibodies, mouse anti-Bax, rabbit anti-Bcl-2, rabbit anti-Caspase-3, rabbit anti-Caspase-8 and rabbit anti-p53, rabbit anti-PARP, mouse anti-MMP-2, mouse anti-MMP-9 and mouse anti-Actin (Boster, Inc. China) for overnight at 4°C.

    Techniques: Western Blot, Expressing