mouse anti glial fibrillary acidic protein gfap  (Boster Bio)


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  • 95
    Name:
    Anti GFAP Antibody
    Description:

    Catalog Number:
    MA1045
    Price:
    99.0
    Category:
    Primary Antibodies
    Reactivity:
    Human Pig Rat
    Applications:
    IF, IHC-P, IHC-F, WB
    Immunogen:
    GFAP from pig spinal cord.
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Boster Bio mouse anti glial fibrillary acidic protein gfap
    Anti GFAP Antibody

    https://www.bioz.com/result/mouse anti glial fibrillary acidic protein gfap/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti glial fibrillary acidic protein gfap - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models"

    Article Title: Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.00722

    Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.
    Figure Legend Snippet: Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.

    Techniques Used: Expressing, Marker

    2) Product Images from "Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy"

    Article Title: Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-18416-5

    Localization of GHRH in the human neocortex. ( a ) GHRH (green) co-localized with MAP2-positive (red) neurons in the human neocortex. ( b ) GHRH (green) and GFAP (red) were not co-expressed in astrocytes in the human neocortex. DAPI (blue) indicates the cell nuclei. The white squares indicate positive cells, and the scale bar represents 50 μm.
    Figure Legend Snippet: Localization of GHRH in the human neocortex. ( a ) GHRH (green) co-localized with MAP2-positive (red) neurons in the human neocortex. ( b ) GHRH (green) and GFAP (red) were not co-expressed in astrocytes in the human neocortex. DAPI (blue) indicates the cell nuclei. The white squares indicate positive cells, and the scale bar represents 50 μm.

    Techniques Used:

    Localization of GHRH in the mouse cortex and hippocampus. ( a , d) GHRH (green) co-localized with MAP2-positive (red) neurons in the mouse cortex and hippocampus. ( b , e) GHRH (green) and GFAP (red) were not co-expressed in astrocytes in the mouse cortex and hippocampus. DAPI (blue) indicates the cell nuclei. The white squares indicate positive cells, and the scale bar represents 50 μm. ( b ) GHRH (green) was localized in both the cortex and the whole hippocampus, and cell nuclei were counterstained with DAPI (blue). Scale bar = 200 μm.
    Figure Legend Snippet: Localization of GHRH in the mouse cortex and hippocampus. ( a , d) GHRH (green) co-localized with MAP2-positive (red) neurons in the mouse cortex and hippocampus. ( b , e) GHRH (green) and GFAP (red) were not co-expressed in astrocytes in the mouse cortex and hippocampus. DAPI (blue) indicates the cell nuclei. The white squares indicate positive cells, and the scale bar represents 50 μm. ( b ) GHRH (green) was localized in both the cortex and the whole hippocampus, and cell nuclei were counterstained with DAPI (blue). Scale bar = 200 μm.

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus
    Article Snippet: .. After three washes with PBS each for 5 minutes, the slices were blocked with 5% bovine serum albumin for 30 minutes at 37°C, and incubated in mouse anti-rat GFAP antibody (astrocyte marker, monoclonal antibody; 1:100; Boster Biological Technology Co., Ltd., Wuhan, China) overnight at 4°C. ..

    Article Title: OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats
    Article Snippet: .. The sections were incubated overnight at 4 °C with the following primary antibodies: anti-OTULIN rabbit antibody (bs-14689R, Bioss Co., Beijing, China, 1:50), anti-NeuN mouse antibody (MAB377, Millipore Co., Germany, 1:200), anti-Iba-1 goat antibody (NB100-1028, Novus Co., USA, 1:200), anti-GFAP mouse antibody (A00213, BOSTER Co.), and anti-NF-κB p65 rabbit antibody p65 (#8242, Cell Signaling Technology, USA). ..

    Article Title: Microglia-mediated chronic psoriatic itch induced by imiquimod
    Article Snippet: .. After nonspecific blocking in goat serum, the sections were incubated with primary antibodies against IBA-1 (1:100, Abcam, ab178847) and GFAP (1:100, Boster, BM4287) at 4°C overnight. ..

    Article Title: A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats
    Article Snippet: .. Then, brain slices were blocked with 5% donkey or goat serum for 1 h and incubated overnight at 4°C with primary antibodies: ABIN1 (bs-9568R, Bioss, China, 1 : 50), NeuN (MAB377, Millipore, Germany, 1 : 200), A20 (3A11G6, Proteintech, USA, 1 : 100), Iba-1 (NB100-1028, Novus, USA, 1 : 50), and GFAP (BM0055, Boster, China, 1 : 100). ..

    Article Title: Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression
    Article Snippet: .. Then, the sections were incubated with a mixture of primary antibody buffer containing a Drp1 antibody (1:500, Abcam, USA), microtubule-associated protein 2 (MAP 2) antibody (1:500, Sysy, Germany), and glial fibrillary acidic protein (GFAP) antibody (1:200, Boster, China) overnight at 4 °C. ..

    Marker:

    Article Title: Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus
    Article Snippet: .. After three washes with PBS each for 5 minutes, the slices were blocked with 5% bovine serum albumin for 30 minutes at 37°C, and incubated in mouse anti-rat GFAP antibody (astrocyte marker, monoclonal antibody; 1:100; Boster Biological Technology Co., Ltd., Wuhan, China) overnight at 4°C. ..

    Blocking Assay:

    Article Title: Microglia-mediated chronic psoriatic itch induced by imiquimod
    Article Snippet: .. After nonspecific blocking in goat serum, the sections were incubated with primary antibodies against IBA-1 (1:100, Abcam, ab178847) and GFAP (1:100, Boster, BM4287) at 4°C overnight. ..

    Immunofluorescence:

    Article Title: PPAR-γ Mediates Ta-VNS-Induced Angiogenesis and Subsequent Functional Recovery after Experimental Stroke in Rats
    Article Snippet: .. Immunofluorescence The brain tissues of sacrificed rats at 28 d after reperfusion were fixed with 4% paraformaldehyde, cut into coronal sections, permeabilized with 0.4% Triton X-100, and stained overnight at 4°C with primary antibodies diluted in PBS. (anti-PPAR-γ (1 : 50, Abcam), anti-NeuN (1 : 200, Millipore), anti-GFAP (1 : 100, Boster), anti-CD31 (1 : 50, R & D systems), anti-Ki67 (1 : 50, Abcam), and anti-FLAG (1 : 50, Proteintech)). ..

    Staining:

    Article Title: PPAR-γ Mediates Ta-VNS-Induced Angiogenesis and Subsequent Functional Recovery after Experimental Stroke in Rats
    Article Snippet: .. Immunofluorescence The brain tissues of sacrificed rats at 28 d after reperfusion were fixed with 4% paraformaldehyde, cut into coronal sections, permeabilized with 0.4% Triton X-100, and stained overnight at 4°C with primary antibodies diluted in PBS. (anti-PPAR-γ (1 : 50, Abcam), anti-NeuN (1 : 200, Millipore), anti-GFAP (1 : 100, Boster), anti-CD31 (1 : 50, R & D systems), anti-Ki67 (1 : 50, Abcam), and anti-FLAG (1 : 50, Proteintech)). ..

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    Boster Bio mouse anti glial fibrillary acidic protein gfap
    Localization of <t>OLFM3</t> in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and <t>GFAP</t> (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.
    Mouse Anti Glial Fibrillary Acidic Protein Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti glial fibrillary acidic protein gfap/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti glial fibrillary acidic protein gfap - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    95
    Boster Bio rabbit anti gfap
    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of <t>C3</t> in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), <t>GFAP</t> (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.
    Rabbit Anti Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti gfap/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti gfap - by Bioz Stars, 2021-09
    95/100 stars
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    92
    Boster Bio rabbit anti glial fibrillary acidic protein gfap
    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and <t>microvascular</t> endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express <t>GFAP</t> (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.
    Rabbit Anti Glial Fibrillary Acidic Protein Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti glial fibrillary acidic protein gfap/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glial fibrillary acidic protein gfap - by Bioz Stars, 2021-09
    92/100 stars
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    Image Search Results


    Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models

    doi: 10.3389/fcell.2020.00722

    Figure Lengend Snippet: Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.

    Article Snippet: The following primary antibodies were used: rabbit anti-OLFM3 (1:50, Proteintech, Wuhan, China), mouse anti-glial fibrillary acidic protein (GFAP) (1:50, Boster Bioengineering, Wuhan, China), guinea pig anti-microtubule-associated protein 2 (MAP2) (1:200, Sysy, Göttingen, Germany), guinea pig anti-vGlut1(1:200, Sysy, Göttingen, Germany), mouse anti-PSD95 (1:100, Abcam, United States), mouse anti-GluA1 (1:50, Santa Cruz Biotechnology, United States), mouse anti-GluA2 (1:100, Abcam, United States), Alexa Fluor 488-conjugated goat anti-rabbit IgG antibody (1:50, Zhongshan Golden Bridge, Inc., Beijing, China), Alexa Fluor 594-conjugated goat anti-mouse IgG antibody (1:200, Zhongshan Golden Bridge Inc., Beijing, China), and Alexa Fluor 633-conjugated goat anti-guinea pig IgG antibody (1:50, Abcam, United States).

    Techniques: Expressing, Marker

    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Western Blot

    A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Cell Culture, Staining, Immunohistochemistry, Expressing, Western Blot

    Graphene oxide inhibits the cell viability and promotes the differentiation of U251 GSCs. a U251 cells were cultured in a serum-free environment. Sphere morphology was photographed using light microscopy. Scale bar = 100 μm. b The expression of SOX2, CD133 and OCT4 in glioblastoma stem-like cells was increased during different periods. c Morphological appearance of U251 GSCs with or without treatment with GO for 2 days. The GSC spheres treated with GO showed adherent growth. Scale bar = 100 μm. d An MTT assay showed the cell viability of U251 GSCs with or without treatment with different dosages of GO for 2, 4, and 6 days. e Quantification of the mRNA levels of the stem cell markers SOX2 and differentiation markers (GFAP and TUJ1) in U251 GSCs with or without treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Journal: Journal of Translational Medicine

    Article Title: Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms

    doi: 10.1186/s12967-020-02359-z

    Figure Lengend Snippet: Graphene oxide inhibits the cell viability and promotes the differentiation of U251 GSCs. a U251 cells were cultured in a serum-free environment. Sphere morphology was photographed using light microscopy. Scale bar = 100 μm. b The expression of SOX2, CD133 and OCT4 in glioblastoma stem-like cells was increased during different periods. c Morphological appearance of U251 GSCs with or without treatment with GO for 2 days. The GSC spheres treated with GO showed adherent growth. Scale bar = 100 μm. d An MTT assay showed the cell viability of U251 GSCs with or without treatment with different dosages of GO for 2, 4, and 6 days. e Quantification of the mRNA levels of the stem cell markers SOX2 and differentiation markers (GFAP and TUJ1) in U251 GSCs with or without treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Article Snippet: We performed incubation with anti-CD133 (1:200; PB0168, Boster) and anti-GFAP (1:200; BA0056, Boster) antibodies overnight, which was followed by incubation with secondary antibody for 1 h. The nuclear DNA was labeled with DAPI.

    Techniques: Cell Culture, Light Microscopy, Expressing, MTT Assay

    Graphene oxide reduces the expression of stem cell markers and promotes the differentiation of GSCs. a Quantification of the mRNA levels of stem cell markers SOX2 and CD133 in GSCs with or without treatment with GO. b The intracellular expression of the differentiation marker GFAP after treatment with 50 μg/ml GO was examined using immunofluorescence staining. Scale bar = 100 μm. c The expression level of the stem cell marker CD133 in cells treated with different concentrations of GO was detected by immunofluorescence staining. Scale bar = 50 μm. d , e Representative immunoblots and relative quantification of OCT4, SOX2, TUJ1 and GFAP in GSCs after treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Journal: Journal of Translational Medicine

    Article Title: Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms

    doi: 10.1186/s12967-020-02359-z

    Figure Lengend Snippet: Graphene oxide reduces the expression of stem cell markers and promotes the differentiation of GSCs. a Quantification of the mRNA levels of stem cell markers SOX2 and CD133 in GSCs with or without treatment with GO. b The intracellular expression of the differentiation marker GFAP after treatment with 50 μg/ml GO was examined using immunofluorescence staining. Scale bar = 100 μm. c The expression level of the stem cell marker CD133 in cells treated with different concentrations of GO was detected by immunofluorescence staining. Scale bar = 50 μm. d , e Representative immunoblots and relative quantification of OCT4, SOX2, TUJ1 and GFAP in GSCs after treatment with 0, 5, 12.5, 25 and 50 μg/ml GO respectively. * p

    Article Snippet: We performed incubation with anti-CD133 (1:200; PB0168, Boster) and anti-GFAP (1:200; BA0056, Boster) antibodies overnight, which was followed by incubation with secondary antibody for 1 h. The nuclear DNA was labeled with DAPI.

    Techniques: Expressing, Marker, Immunofluorescence, Staining, Western Blot

    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Journal: Neural Regeneration Research

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression

    doi: 10.4103/1673-5374.244794

    Figure Lengend Snippet: Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Article Snippet: Astrocytes and brain microvascular endothelial cells were labeled with rabbit anti-glial fibrillary acidic protein (GFAP) (1:100; Boster) and rabbit anti-PECAM-1/CD31 (1:100; Boster) antibodies.

    Techniques: Light Microscopy