mouse anti digoxigenin  (Millipore)


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  • 99
    Name:
    Anti Digoxigenin
    Description:
    Contents Lyophilizate
    Catalog Number:
    11333062910
    Price:
    None
    Applications:
    Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using: . ELISA. Immunohistocytochemistry. In situ hybridization. Western blot. Immunofluorescence staining. FISH (fluorescent in situ hybridization)
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    Structured Review

    Millipore mouse anti digoxigenin
    Use of PLA for the study of a protein-mRNA interaction. (A) H1299 cells were transfected with EBNA1 and analyzed by RNA in situ hybridization-immunofluorescence (rISH-IF) to verify the specificity of the EBNA1 <t>-digoxigenin</t> probe and to validate the detection of probe-mRNA complexes using a mouse anti-digoxigenin antibody. Immunocomplexes were detected using an anti-mouse Alexa Fluor ® 568-conjugated secondary antibody, revealing the accumulation of EBNA1 mRNA in the nucleus. EBNA1 mRNA is depicted in red. ( B ) Immunofluorescence (IF) was performed in H1299 cells using a rabbit anti-nucleolin antibody to set up the appropriate conditions for detection of endogenous NCL. The expected labelling of NCL in the nucleolus was confirmed. ( C ) PLA in EBNA1 -transfected H1299 cells using mouse anti-digoxigenin and rabbit anti-nucleolin tested in (A) and (B). Anti-rabbit and anti-mouse Ig PLA probes were used following the manufacturer’s protocol to generate PLA complexes depicted as white dots. Each dot represents an interaction between NCL and EBNA1 mRNA. ( D ) The EBV-transformed B-cell line B95-8 was tested for endogenous NCL- EBNA1 mRNA interaction under the same conditions used in ( C ). PLA uncovered this interaction in the nuclear compartment as in EBNA1 -transfected H1299 cells shown in ( C ). Scale bars represent 10 µm.
    Contents Lyophilizate
    https://www.bioz.com/result/mouse anti digoxigenin/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    mouse anti digoxigenin - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules"

    Article Title: In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules

    Journal: Molecules

    doi: 10.3390/molecules23123124

    Use of PLA for the study of a protein-mRNA interaction. (A) H1299 cells were transfected with EBNA1 and analyzed by RNA in situ hybridization-immunofluorescence (rISH-IF) to verify the specificity of the EBNA1 -digoxigenin probe and to validate the detection of probe-mRNA complexes using a mouse anti-digoxigenin antibody. Immunocomplexes were detected using an anti-mouse Alexa Fluor ® 568-conjugated secondary antibody, revealing the accumulation of EBNA1 mRNA in the nucleus. EBNA1 mRNA is depicted in red. ( B ) Immunofluorescence (IF) was performed in H1299 cells using a rabbit anti-nucleolin antibody to set up the appropriate conditions for detection of endogenous NCL. The expected labelling of NCL in the nucleolus was confirmed. ( C ) PLA in EBNA1 -transfected H1299 cells using mouse anti-digoxigenin and rabbit anti-nucleolin tested in (A) and (B). Anti-rabbit and anti-mouse Ig PLA probes were used following the manufacturer’s protocol to generate PLA complexes depicted as white dots. Each dot represents an interaction between NCL and EBNA1 mRNA. ( D ) The EBV-transformed B-cell line B95-8 was tested for endogenous NCL- EBNA1 mRNA interaction under the same conditions used in ( C ). PLA uncovered this interaction in the nuclear compartment as in EBNA1 -transfected H1299 cells shown in ( C ). Scale bars represent 10 µm.
    Figure Legend Snippet: Use of PLA for the study of a protein-mRNA interaction. (A) H1299 cells were transfected with EBNA1 and analyzed by RNA in situ hybridization-immunofluorescence (rISH-IF) to verify the specificity of the EBNA1 -digoxigenin probe and to validate the detection of probe-mRNA complexes using a mouse anti-digoxigenin antibody. Immunocomplexes were detected using an anti-mouse Alexa Fluor ® 568-conjugated secondary antibody, revealing the accumulation of EBNA1 mRNA in the nucleus. EBNA1 mRNA is depicted in red. ( B ) Immunofluorescence (IF) was performed in H1299 cells using a rabbit anti-nucleolin antibody to set up the appropriate conditions for detection of endogenous NCL. The expected labelling of NCL in the nucleolus was confirmed. ( C ) PLA in EBNA1 -transfected H1299 cells using mouse anti-digoxigenin and rabbit anti-nucleolin tested in (A) and (B). Anti-rabbit and anti-mouse Ig PLA probes were used following the manufacturer’s protocol to generate PLA complexes depicted as white dots. Each dot represents an interaction between NCL and EBNA1 mRNA. ( D ) The EBV-transformed B-cell line B95-8 was tested for endogenous NCL- EBNA1 mRNA interaction under the same conditions used in ( C ). PLA uncovered this interaction in the nuclear compartment as in EBNA1 -transfected H1299 cells shown in ( C ). Scale bars represent 10 µm.

    Techniques Used: Proximity Ligation Assay, Transfection, RNA In Situ Hybridization, Immunofluorescence, Transformation Assay

    2) Product Images from "In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules"

    Article Title: In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules

    Journal: Molecules

    doi: 10.3390/molecules23123124

    Use of PLA for the study of a protein-mRNA interaction. (A) H1299 cells were transfected with EBNA1 and analyzed by RNA in situ hybridization-immunofluorescence (rISH-IF) to verify the specificity of the EBNA1 -digoxigenin probe and to validate the detection of probe-mRNA complexes using a mouse anti-digoxigenin antibody. Immunocomplexes were detected using an anti-mouse Alexa Fluor ® 568-conjugated secondary antibody, revealing the accumulation of EBNA1 mRNA in the nucleus. EBNA1 mRNA is depicted in red. ( B ) Immunofluorescence (IF) was performed in H1299 cells using a rabbit anti-nucleolin antibody to set up the appropriate conditions for detection of endogenous NCL. The expected labelling of NCL in the nucleolus was confirmed. ( C ) PLA in EBNA1 -transfected H1299 cells using mouse anti-digoxigenin and rabbit anti-nucleolin tested in (A) and (B). Anti-rabbit and anti-mouse Ig PLA probes were used following the manufacturer’s protocol to generate PLA complexes depicted as white dots. Each dot represents an interaction between NCL and EBNA1 mRNA. ( D ) The EBV-transformed B-cell line B95-8 was tested for endogenous NCL- EBNA1 mRNA interaction under the same conditions used in ( C ). PLA uncovered this interaction in the nuclear compartment as in EBNA1 -transfected H1299 cells shown in ( C ). Scale bars represent 10 µm.
    Figure Legend Snippet: Use of PLA for the study of a protein-mRNA interaction. (A) H1299 cells were transfected with EBNA1 and analyzed by RNA in situ hybridization-immunofluorescence (rISH-IF) to verify the specificity of the EBNA1 -digoxigenin probe and to validate the detection of probe-mRNA complexes using a mouse anti-digoxigenin antibody. Immunocomplexes were detected using an anti-mouse Alexa Fluor ® 568-conjugated secondary antibody, revealing the accumulation of EBNA1 mRNA in the nucleus. EBNA1 mRNA is depicted in red. ( B ) Immunofluorescence (IF) was performed in H1299 cells using a rabbit anti-nucleolin antibody to set up the appropriate conditions for detection of endogenous NCL. The expected labelling of NCL in the nucleolus was confirmed. ( C ) PLA in EBNA1 -transfected H1299 cells using mouse anti-digoxigenin and rabbit anti-nucleolin tested in (A) and (B). Anti-rabbit and anti-mouse Ig PLA probes were used following the manufacturer’s protocol to generate PLA complexes depicted as white dots. Each dot represents an interaction between NCL and EBNA1 mRNA. ( D ) The EBV-transformed B-cell line B95-8 was tested for endogenous NCL- EBNA1 mRNA interaction under the same conditions used in ( C ). PLA uncovered this interaction in the nuclear compartment as in EBNA1 -transfected H1299 cells shown in ( C ). Scale bars represent 10 µm.

    Techniques Used: Proximity Ligation Assay, Transfection, RNA In Situ Hybridization, Immunofluorescence, Transformation Assay

    Related Articles

    Proximity Ligation Assay:

    Article Title: p53-mediated suppression of BiP triggers BIK-induced apoptosis during prolonged endoplasmic reticulum stress
    Article Snippet: .. Samples were briefly washed with wash buffer (2X SSC, 10% formamide), further washed twice with hybridization buffer for 20 min and once with PBS for 20 min at 37 °C, followed by the above-described PLA and IF protocols using 1:200 dilution of anti-digoxigenin mouse mAb (Sigma-Aldrich) and 1:500 for anti-p53 CM-1 rabbit pAb in blocking buffer. .. Images were obtained either with Axiovert 200M microscopy and AxioVision software (Carl Zeiss Vision, Oberkochen, Germany) or LSM 800 airyscan confocal microscopy and Zen 2.1 (blue edition) software (Carl Zeiss Microscopy GmbH, Oberkochen, Germany).

    Blocking Assay:

    Article Title: p53-mediated suppression of BiP triggers BIK-induced apoptosis during prolonged endoplasmic reticulum stress
    Article Snippet: .. Samples were briefly washed with wash buffer (2X SSC, 10% formamide), further washed twice with hybridization buffer for 20 min and once with PBS for 20 min at 37 °C, followed by the above-described PLA and IF protocols using 1:200 dilution of anti-digoxigenin mouse mAb (Sigma-Aldrich) and 1:500 for anti-p53 CM-1 rabbit pAb in blocking buffer. .. Images were obtained either with Axiovert 200M microscopy and AxioVision software (Carl Zeiss Vision, Oberkochen, Germany) or LSM 800 airyscan confocal microscopy and Zen 2.1 (blue edition) software (Carl Zeiss Microscopy GmbH, Oberkochen, Germany).

    Purification:

    Article Title: Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing
    Article Snippet: .. A mouse monoclonal anti-Digoxigenin antibody (Clone 1.71.256) and the purified natural allergen component protein lactalbumin (Bos d 4) were purchased from Sigma-Aldrich (St. Louis, MO). .. All other allergen component proteins were purchased from Indoor Biotechnologies (Charlottesville, VA).

    Incubation:

    Article Title: In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules
    Article Snippet: .. Samples were saturated with PBS 3% BSA for 30 min and incubated with a mouse anti-digoxigenin (1/200, clone DI-22, Sigma) for 2 h at room temperature. .. A goat anti-mouse immunoglobulin G (IgG) secondary antibody conjugated to Alexa Fluor® 568 (Sigma) was used to detect immunocomplexes (1 h at 37 °C) and DAPI was used for nuclear counterstaining under standard conditions.

    Article Title: Transport and Localization Elements in Myelin Basic Protein mRNA
    Article Snippet: .. To visualize digoxigenin-labeled RNA, cells were fixed in 4% paraformaldehyde in PBS and incubated with anti-digoxigenin antibodies as previously described , or using a mouse monoclonal anti-digoxigenin antibody (1:75 dilution), followed by detection with a fluorescein-conjugated goat anti–mouse IgG (Chemicon International). .. MBP was visualized using rabbit polyclonal anti-MBP followed by Texas red-conjugated donkey anti–rabbit IgG ( Jackson ImmunoResearch Laboratories, Inc. ).

    Binding Assay:

    Article Title: Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes
    Article Snippet: .. Probe binding was detected by indirect immunofluorescence analysis with mouse monoclonal anti-digoxigenin antibody (Sigma-Aldrich, 1:300 dilution) and Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody (Invitrogen, 1∶600 dilution), as described previously. .. As a control, 100 µg/ml RNase A was added to the pretreatment buffer before probe hybridization.

    Fluorescence In Situ Hybridization:

    Article Title: HCMV-Infected Cells Maintain Efficient Nucleotide Excision Repair of the Viral Genome while Abrogating Repair of the Host Genome
    Article Snippet: .. Anti-digoxigenin Ab (Sigma) was diluted 1∶1000 in 0.6% cold water fish skin gelatin in TBST and the blot was probed for 1 h at RT. .. The blot was washed at RT for 5 min in TBST, 10 min in TBST with 1% SDS, and three times with TBST for 5 min. Anti-mouse IRdye700 and streptavidin IRdye800 (Rockland) were diluted 1∶4,000 and 1∶20,000, respectively in 0.6% cold water fish skin gelatin in TBST with 0.02% SDS and the blot was incubated 45 min in the dark at RT.

    Immunofluorescence:

    Article Title: Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes
    Article Snippet: .. Probe binding was detected by indirect immunofluorescence analysis with mouse monoclonal anti-digoxigenin antibody (Sigma-Aldrich, 1:300 dilution) and Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody (Invitrogen, 1∶600 dilution), as described previously. .. As a control, 100 µg/ml RNase A was added to the pretreatment buffer before probe hybridization.

    Hybridization:

    Article Title: p53-mediated suppression of BiP triggers BIK-induced apoptosis during prolonged endoplasmic reticulum stress
    Article Snippet: .. Samples were briefly washed with wash buffer (2X SSC, 10% formamide), further washed twice with hybridization buffer for 20 min and once with PBS for 20 min at 37 °C, followed by the above-described PLA and IF protocols using 1:200 dilution of anti-digoxigenin mouse mAb (Sigma-Aldrich) and 1:500 for anti-p53 CM-1 rabbit pAb in blocking buffer. .. Images were obtained either with Axiovert 200M microscopy and AxioVision software (Carl Zeiss Vision, Oberkochen, Germany) or LSM 800 airyscan confocal microscopy and Zen 2.1 (blue edition) software (Carl Zeiss Microscopy GmbH, Oberkochen, Germany).

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