Journal: Nagoya Journal of Medical Science

Article Title: Protective effect of Sasa veitchii extract against all-trans-retinoic acid-induced inhibition of proliferation of cultured human palate cells

doi: 10.18999/nagjms.86.2.223

Figure Lengend Snippet: Involvement with apoptosis and cell cycle by treatment with of SE against at RA in HEPM cells Fig. 3A: Apotracker staining (green) of HEPM cells after treatment with 100 μM at RA and 0.03% SE for 48 h. The nuclei were counterstained with Hoechst 33342 (blue). Scale bar, 50 μm. Fig. 3B: Immunoblotting of HEPM cells after treatment with 100 μM at RA and 0.03% SE for 48 h. β-actin was served as an internal control. *p < 0.05 and ***p < 0.001 versus at RA. at RA: all-trans -retinoic acid HEPM: human embryonic palatal mesenchymal SE: Sasa veitchii extract CCND1: cyclin D1

Article Snippet: Rabbit anti-cleaved caspase-3 polyclonal antibody (1:2,500 dilution; Cell Signaling Technology, Beverly, MA), mouse anti-cyclin D1 (CCND1) monoclonal antibody (1:1,000 dilution; Santa Cruz Biotechnology, Dallas, TX), mouse anti-CCNA (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNB (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNE (1:1,000 dilution; Santa Cruz Biotechnology), and anti-mouse β-actin monoclonal antibody (1:2,500 dilution; MBL, Aichi, Japan) were used as primary antibodies for immunoblotting.

Techniques: Staining, Western Blot, Control

Journal: Nagoya Journal of Medical Science

Article Title: Protective effect of Sasa veitchii extract against all-trans-retinoic acid-induced inhibition of proliferation of cultured human palate cells

doi: 10.18999/nagjms.86.2.223

Figure Lengend Snippet: Proposed mechanism of SE against at RA-induced cell proliferation inhibition ERBB2: Erb-B2 receptor tyrosine kinase 2 JADE1 : jade family PHD finger 1 CCND1: cyclin D1

Article Snippet: Rabbit anti-cleaved caspase-3 polyclonal antibody (1:2,500 dilution; Cell Signaling Technology, Beverly, MA), mouse anti-cyclin D1 (CCND1) monoclonal antibody (1:1,000 dilution; Santa Cruz Biotechnology, Dallas, TX), mouse anti-CCNA (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNB (1:1,000 dilution; Santa Cruz Biotechnology), anti-mouse CCNE (1:1,000 dilution; Santa Cruz Biotechnology), and anti-mouse β-actin monoclonal antibody (1:2,500 dilution; MBL, Aichi, Japan) were used as primary antibodies for immunoblotting.

Techniques: Inhibition

Journal: Current Issues in Molecular Biology

Article Title: 5-AZA Upregulates SOCS3 and PTPN6/SHP1, Inhibiting STAT3 and Potentiating the Effects of AG490 against Primary Effusion Lymphoma Cells

doi: 10.3390/cimb46030156

Figure Lengend Snippet: 5-AZA further inhibits STAT3 activity in PEL cells treated by AG490. BC3 and BCBL-1 cell lines were treated singly or in combination with 5-Azacitidine (5-AZA) and AG490. ( A ) Cell survival was estimated by trypan blue exclusion assay after treatment with AG490 (100 μM), 5-AZA (20 nM), or a combination of both. The histograms represent the mean of the percentage of cell viability relative to the control plus S.D. ( B ) Sub-G1 events as evaluated via FACS analysis in BC3 and BCBL-1 cells. The histograms represent the mean of the percentage of sub G1 of three independent experiments. ( C ) Protein expression level of pSTAT3, STAT3, c-MYC, HSP27, Cyclin D1, as evaluated by Western blot analysis. GAPDH was used as a loading control, and one representative experiment is shown. The histograms represent the densitometric analysis of specific protein and the appropriate control from three experiments. Data are represented as the mean plus S.D. p -value: * < 0.05; ** < 0.01; *** < 0.001; and **** < 0.0001, ns means no significance.

Article Snippet: To evaluate protein expression on Western blot membranes, the following antibodies were used: mouse monoclonal anti-pSTAT3 TYR (1:500) (pY705, BD Biosciences, Franklin Lakes, NJ, USA, #612356), mouse monoclonal anti-STAT3 (1:500) (BD Biosciences, #6101289), rabbit polyclonal anti-DNMT1 (1:1000) (Proteintech Europe, Manchester, UK, #24206-1-AP), rabbit polyclonal anti-PTPN6 (1:250) (Proteintech Europe, Manchester, UK, #24546-1-AP), mouse monoclonal anti-Cyclin D1 (1:100) (Santa Cruz Bio-technology, #sc-8396), rabbit polyclonal anti-c-MYC (1:500) (Proteintech Europe, Manchester, UK, #10828-1-AP), rabbit polyclonal anti-SOCS3 (1:500) (Proteintech Europe, Manchester, UK, #14025-1-AP), rabbit polyclonal anti-HSP27 (1:5000) (Proteintech Europe, Manchester, UK, 1#8284-1-AP), and mouse monoclonal anti-JACK2 (1:100) (Santa Cruz Biotechnology, Dallas, TX, USA, #sc-390539); mouse mono-clonal anti-GAPDH (1:10,000) (Santa Cruz Biotechnology, Dallas, TX, USA, sc-137179) was used as loading control.

Techniques: Activity Assay, Trypan Blue Exclusion Assay, Expressing, Western Blot

Journal: iScience

Article Title: Schisandrin B promotes hepatic differentiation from human umbilical cord mesenchymal stem cells

doi: 10.1016/j.isci.2024.108912

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Cyclin D1 , Proteintech , Cat#60186-1-Ig; RRID: AB_10793718.

Techniques: Recombinant, CCK-8 Assay, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Live Dead Assay, Software

Journal: International Journal of Molecular Sciences

Article Title: miR-195-5p as Regulator of γ-Catenin and Desmosome Junctions in Colorectal Cancer

doi: 10.3390/ijms242317084

Figure Lengend Snippet: miR-195-5p regulated NLK, LEF1 and Cyclin D1 protein expression in all cell lines. WB analysis revealed that the gain of miR-195-5p in Caco2 ( A ) and LoVo ( B ) significantly decreased the expression levels of these key effectors of the Wnt pathway. Data obtained from three independent experiments (n = 3; mean ± SEM) were normalized to housekeeping values. ** p < 0.001, *** p < 0.0001.

Article Snippet: For protein detection, primary antibodies of rabbit monoclonal γ-Catenin (#75550S, Cell Signaling, Technology, Danvers, MA, USA; dilution 1:3000), rabbit polyclonal Desmoglein-2 (ab226184, Abcam, Cambridge, UK; dilution 1:5000), mouse monoclonal Desmocollin-2 (#32-6200, Invitrogen, Carlsbad, CA, USA; dilution 1:500) rabbit polyclonal NLK (#PA5-21877, Invitrogen, Carlsbad, CA, USA; dilution 1:500), mouse polyclonal LEF-1 (#MA1-12420, Invitrogen, Carlsbad, CA, USA; dilution 1:400), mouse monoclonal Cyclin D1 (#MA5-16356, Invitrogen, Carlsbad, CA, USA; dilution 1:1000) and mouse monoclonal ß-Tubulin (sc-166729, Santa Cruz Biotechnology, Inc., Heidelberg, Germany; dilution 1:1000) were used.

Techniques: Expressing