mouse anti brutp  (Thermo Fisher)


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    Name:
    Shandon Grooved Needle Director
    Description:
    Choose 5″ 12 7cm Thermo Scientific Shandon Grooved Needle Director for quality and precision
    Catalog Number:
    1100
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    Applications:
    Anatomical Pathology|Clinical
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    Structured Review

    Thermo Fisher mouse anti brutp
    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and <t>LB1.</t> Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by <t>BrUTP</t> incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Choose 5″ 12 7cm Thermo Scientific Shandon Grooved Needle Director for quality and precision
    https://www.bioz.com/result/mouse anti brutp/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brutp - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells"

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    Journal: Nucleus

    doi: 10.1080/19491034.2015.1004256

    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Figure Legend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Techniques Used: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization

    2) Product Images from "Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells"

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    Journal: Nucleus

    doi: 10.1080/19491034.2015.1004256

    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Figure Legend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Techniques Used: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization

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    Staining:

    Article Title: Kindlin-1 promotes pulmonary breast cancer metastasis
    Article Snippet: .. Samples were stained with APC-conjugated anti-β1 integrin (1:100; eBioscience), anti-β1 integrin (clone 9EG7, 5 μg/ml; BD Biosciences) or Alexa Fluor 488-conjugated anti-VCAM-1 (10 μg/ml; AbD Serotec) antibodies and the viability dye eFluor 506 (1:200; eBioscience). .. Alexa Fluor 647-conjugated anti-rat IgG (1:200; eBioscience) secondary antibody was used for 9EG7 staining.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Leptin receptor-expressing bone marrow stromal cells are myofibroblasts in primary myelofibrosis
    Article Snippet: .. Rat-anti-CD41 (eBioscience, eBioMWReg30, Cat13-0411, 1:100) was used as primary antibody. .. Reticulin staining Bone sections prepared as above were stained with Reticulin Stain Kit (Polysciences, Inc.) per manufacture’s instruction.

    Activity Assay:

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: .. In brief, sections were dewaxed and rehydrated, endogenous peroxidase activity was blocked by 0.3% H2 O2 /methanol for 20 min, and heat-induced antigen retrieval was obtained by microwave or thermostatic bath treatment using EDTA buffer, pH 8.0, followed by 1-h incubation with primary antibody rabbit anti-CD3 (1:100; Dako) or rat anti-FoxP3 (1:100; eBioscience). .. Signal was revealed by using Real EnVision Rabbit-HRP (Dako) or Rat-on-Mouse HRP-Polymer (Biocare Medical) detection system followed by diaminobenzidine and nuclei counterstained with hematoxylin.

    Incubation:

    Article Title: Human NK cell development requires CD56-mediated motility and formation of the developmental synapse
    Article Snippet: .. NK cells were incubated with antibodies to CD56 (clone HCD56, AlexaFluor 647 conjugated, Biolegend, 1:100) and CD34 (clone 4H11, PE conjugated, eBioscience, 1:100). .. For FACS analysis following IL-15 culture and CD34+ differentiation, a 10-colour flow cytometry panel was designed ( ).

    Article Title: Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects
    Article Snippet: .. In brief, sections were dewaxed and rehydrated, endogenous peroxidase activity was blocked by 0.3% H2 O2 /methanol for 20 min, and heat-induced antigen retrieval was obtained by microwave or thermostatic bath treatment using EDTA buffer, pH 8.0, followed by 1-h incubation with primary antibody rabbit anti-CD3 (1:100; Dako) or rat anti-FoxP3 (1:100; eBioscience). .. Signal was revealed by using Real EnVision Rabbit-HRP (Dako) or Rat-on-Mouse HRP-Polymer (Biocare Medical) detection system followed by diaminobenzidine and nuclei counterstained with hematoxylin.

    Article Title: Deletion of the Syncytin A receptor Ly6e impairs syncytiotrophoblast fusion and placental morphogenesis causing embryonic lethality in mice
    Article Snippet: .. Cells were then fixed for 15 minutes at room temperature in 2% PFA, 1x PBS followed by incubation in rhodamine-phalloidin (1:100; Molecular Probes, Invitrogen, USA). .. Cells were then washed several times in 1x PBS and counterstained in Hoechst 33342 (1:1000; Molecular Probes, Invitrogen, USA) and mounted on slides in Fluorescent Mounting Medium (Dako, USA).

    Expressing:

    Article Title: CFTR Inhibition Provokes an Inflammatory Response Associated with an Imbalance of the Annexin A1 Pathway
    Article Snippet: .. In some cases, AnxA1 expression in mouse peritoneal neutrophils (elicited at 4 hours postzymosan and/or CFTR inhibitors) was also determined using the same permeabilization protocol, and a rabbit anti-AnxA1 antibody (Ab; 1:100: Invitrogen; Calne UK) or control rabbit IgG (Dako; Cambridgeshire, UK). .. Flow cytometry was performed as above, identifying the predominant ( > 85%) neutrophil population in the lavage fluids by its scatter characteristics.

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  • 90
    Thermo Fisher brutp
    VP2 L124A expression decreases viral transcription and replication of viroplasms. (A) At 24 hpt, for expression of wt VP2 (upper row) and VP2 L124A (lower row), BSR-T7 cells were RV infected (MOI, 75 VFU/cell). At 4 <t>hpi,</t> cells were treated with 10 μM actinomycin D for 30 min and then transfected for 1 h with 1 mM <t>BrUTP</t> (+BrUTP) or not (−BrUTP) before fixation. Cells were immunostained for BrUTP incorporation (anti-BrdU, green) and viroplasm detection (anti-NSP5, red). Nuclei were stained with DAPI (blue). Scale bar is 5 μm. (B) Confocal immunofluorescence of BSR-T7 cells alone, expressing wt VP2 or VP2 L124A, followed at 24 hpt by RV infection (MOI, 150 VFU/cell). At 5 hpi, cells were fixed and immunostained for detection of dsRNA (anti-dsRNA, red) and viroplasms (anti-NSP5, green). Nuclei were stained with DAPI (blue). N.T., nontransfected cells; a.u., arbitrary units. Scale bar is 5 μm. (C) Intensity profile plot of dsRNA (red line) and viroplasms (green line) of the indicated linear region of interest of cells from panel B.
    Brutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    90/100 stars
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    VP2 L124A expression decreases viral transcription and replication of viroplasms. (A) At 24 hpt, for expression of wt VP2 (upper row) and VP2 L124A (lower row), BSR-T7 cells were RV infected (MOI, 75 VFU/cell). At 4 hpi, cells were treated with 10 μM actinomycin D for 30 min and then transfected for 1 h with 1 mM BrUTP (+BrUTP) or not (−BrUTP) before fixation. Cells were immunostained for BrUTP incorporation (anti-BrdU, green) and viroplasm detection (anti-NSP5, red). Nuclei were stained with DAPI (blue). Scale bar is 5 μm. (B) Confocal immunofluorescence of BSR-T7 cells alone, expressing wt VP2 or VP2 L124A, followed at 24 hpt by RV infection (MOI, 150 VFU/cell). At 5 hpi, cells were fixed and immunostained for detection of dsRNA (anti-dsRNA, red) and viroplasms (anti-NSP5, green). Nuclei were stained with DAPI (blue). N.T., nontransfected cells; a.u., arbitrary units. Scale bar is 5 μm. (C) Intensity profile plot of dsRNA (red line) and viroplasms (green line) of the indicated linear region of interest of cells from panel B.

    Journal: Journal of Virology

    Article Title: Conserved Rotavirus NSP5 and VP2 Domains Interact and Affect Viroplasm

    doi: 10.1128/JVI.01965-19

    Figure Lengend Snippet: VP2 L124A expression decreases viral transcription and replication of viroplasms. (A) At 24 hpt, for expression of wt VP2 (upper row) and VP2 L124A (lower row), BSR-T7 cells were RV infected (MOI, 75 VFU/cell). At 4 hpi, cells were treated with 10 μM actinomycin D for 30 min and then transfected for 1 h with 1 mM BrUTP (+BrUTP) or not (−BrUTP) before fixation. Cells were immunostained for BrUTP incorporation (anti-BrdU, green) and viroplasm detection (anti-NSP5, red). Nuclei were stained with DAPI (blue). Scale bar is 5 μm. (B) Confocal immunofluorescence of BSR-T7 cells alone, expressing wt VP2 or VP2 L124A, followed at 24 hpt by RV infection (MOI, 150 VFU/cell). At 5 hpi, cells were fixed and immunostained for detection of dsRNA (anti-dsRNA, red) and viroplasms (anti-NSP5, green). Nuclei were stained with DAPI (blue). N.T., nontransfected cells; a.u., arbitrary units. Scale bar is 5 μm. (C) Intensity profile plot of dsRNA (red line) and viroplasms (green line) of the indicated linear region of interest of cells from panel B.

    Article Snippet: At 4.5 hpi, cells were transfected with a mixture containing 1 mM BrUTP, 10 μM actinomycin D, and 20 μl of Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) in 500 μl Opti-MEM reduced serum medium (ThermoFisher Scientific) and incubated for 1 h at 37°C and 5% CO2).

    Techniques: Expressing, Infection, Transfection, Staining, Immunofluorescence