mouse anti brutp  (Thermo Fisher)


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    Name:
    BrUPT 5 Bromouridine 5 Triphosphate 10 mM in TE buffer
    Description:
    BrUTP is an excellent substrate for RNA polymerase and has been used to monitor nucleolar transcription in situ BrUTP can be detected with anti BrdU antibodies
    Catalog Number:
    b21551
    Price:
    None
    Category:
    Oligos Primers Probes Nucleotides
    Applications:
    Cell Analysis|Cell Proliferation|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher mouse anti brutp
    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and <t>LB1.</t> Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by <t>BrUTP</t> incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    BrUTP is an excellent substrate for RNA polymerase and has been used to monitor nucleolar transcription in situ BrUTP can be detected with anti BrdU antibodies
    https://www.bioz.com/result/mouse anti brutp/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brutp - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells"

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    Journal: Nucleus

    doi: 10.1080/19491034.2015.1004256

    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Figure Legend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Techniques Used: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization

    2) Product Images from "Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells"

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    Journal: Nucleus

    doi: 10.1080/19491034.2015.1004256

    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Figure Legend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Techniques Used: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization

    Related Articles

    Generated:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Sequencing:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Clone Assay:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    In Vitro:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Purification:

    Article Title: Z Proteins of New World Arenaviruses Bind RIG-I and Interfere with Type I Interferon Induction ▿
    Article Snippet: Transfections were performed with either calcium phosphate or Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. .. 5′-Triphosphate RNA (5′pppRNA) was generated from an 80-bp xenopus elongation factor 1α sequence cloned into pTRI by in vitro transcription using a T7 MEGAshortscript kit and purified using a MEGAclear kit (Ambion, Austin, TX). .. The cell monolayer was washed twice with ice-cold phosphate-buffered saline (PBS; Sigma-Aldrich) and RNA extracted using an RNeasy kit (Qiagen, Hilden, Germany).

    Infection:

    Article Title: Development of an Insect Vector Cell Culture and RNA Interference System To Investigate the Functional Role of Fijivirus Replication Protein
    Article Snippet: VCMs growing on a coverslip and infected with SRBSDV were fixed in 4% paraformaldehyde 48 h postinoculation (p.i.), immunostained with P10-specific IgG conjugated to FITC (P10-FITC) and P9-1-specific IgG conjugated to rhodamine (P9-1–rhodamine), and then examined with a Leica TCS SP5 inverted confocal microscope, as described previously ( ). .. For determining whether the viroplasms are the sites of viral RNA synthesis, VCMs on a coverslip were either mock infected or infected with SRBSDV and then treated with actinomycin D (Sigma) for 1 h at 45 h p.i. and incubated with bromouridine 5′-triphosphate (BrUTP) for 2 h via the use of Cellfectin (Invitrogen). .. The samples were fixed 48 h p.i., stained with monoclonal anti-bromodeoxyuridine (anti-BrdU; Sigma), and then treated with anti-mouse IgG conjugated to FITC (Invitrogen) and P9-1–rhodamine for imaging with confocal microscopy, as described previously ( ).

    Incubation:

    Article Title: Development of an Insect Vector Cell Culture and RNA Interference System To Investigate the Functional Role of Fijivirus Replication Protein
    Article Snippet: VCMs growing on a coverslip and infected with SRBSDV were fixed in 4% paraformaldehyde 48 h postinoculation (p.i.), immunostained with P10-specific IgG conjugated to FITC (P10-FITC) and P9-1-specific IgG conjugated to rhodamine (P9-1–rhodamine), and then examined with a Leica TCS SP5 inverted confocal microscope, as described previously ( ). .. For determining whether the viroplasms are the sites of viral RNA synthesis, VCMs on a coverslip were either mock infected or infected with SRBSDV and then treated with actinomycin D (Sigma) for 1 h at 45 h p.i. and incubated with bromouridine 5′-triphosphate (BrUTP) for 2 h via the use of Cellfectin (Invitrogen). .. The samples were fixed 48 h p.i., stained with monoclonal anti-bromodeoxyuridine (anti-BrdU; Sigma), and then treated with anti-mouse IgG conjugated to FITC (Invitrogen) and P9-1–rhodamine for imaging with confocal microscopy, as described previously ( ).

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Article Title: A CSB-PAF1C axis restores processive transcription elongation after DNA damage repair
    Article Snippet: After this treatment, cells were either mock-treated or irradiated with UV-C light (7 J/m2 ). .. Cells were then incubated in conditioned media for different periods of time (0, 3, 8, 24h) before being incubated with 2 mM bromouridine (Bru) at 37°C for a 30 min. .. The cells were then lysed in TRIzol reagent (Invitrogen) and Bru-containing RNA isolated as previously described ( ). cDNA libraries were made from the Bru-labeled RNA using the Illumina TruSeq library kit and paired-end 151 bp sequenced using the Illumina NovaSeq platform at the University of Michigan DNA Sequencing Core.

    Transfection:

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Immunostaining:

    Article Title: New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells
    Article Snippet: Infection was allowed to proceed for 34.5, 58.5, or 82.5 h, and cells were then treated for 30 min with actinomycin D (Sigma) to inhibit cellular RNA polymerase II. .. Cells were transfected with bromouridine 5′-triphosphate (BrUTP) in the presence of Cellfectin (Invitrogen), according to the manufacturer's instructions, and were incubated for 60 min before fixation and immunostaining. .. BrUTP-labeled viral RNAs were stained with anti-bromodeoxyuridine (BrdU) from mouse (Sigma), followed by anti-mouse IgG conjugated to rhodamine (Sigma).

    Polymerase Chain Reaction:

    Article Title: Hypothalamic–Pituitary–Thyroid Axis Crosstalk With the Hypothalamic–Pituitary–Gonadal Axis and Metabolic Regulation in the Eurasian Tree Sparrow During Mating and Non-mating Periods
    Article Snippet: The PCR primers ( ) were designed in the conserved regions of corresponding gene sequences deposited in GeneBank of other passerines such as zebra finch (Taeniopygia suttata ) and white-throated sparrow (Zonotrichia albicollis ) and obtained the open reading frames for Dio2, Dio3, TRH, TSH , and GnRH-I in ETSs (the nucleotide sequences for each gene were deposited in NCBI, ) by aligning the corresponding sequences using Primer Premier 5.0 (PREMIER Biosoft International, Palo Alto, CA, USA). .. All PCR amplifications were performed in a 50-μL reaction mixture containing 2.0 μL cDNA (200 ng/μL), 5.0 μL mixed dNTPs (2.5 mM each), 10.0 μL 5 × Fast Pfu Buffer and 2.0 μL forward and reverse primers (10 mM), respectively, 1 μL Trans Start FastPfu DNA polymerase (2.5 U/μL), and 28.0 μL sterile water. .. qPCR of Dio2, Dio3, TRH, TSH, GnRH-I , and GnIH for the ETS The qPCR reactions were set up using the TransStrat Top Green qPCR SuperMix (Quanshijin, Beijing, China).

    other:

    Article Title: An exo probe-based recombinase polymerase amplification assay for the rapid detection of porcine parvovirus.
    Article Snippet: Recombinase polymerase amplification (RPA), an isothermal amplification technology, has been developed as an alternative to PCR in pathogen detection.

    Article Title: A Proximity Ligation-Based Method to Detect RNA-DNA Association
    Article Snippet: 5-Bromouridine (BrU).

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    Thermo Fisher mouse anti brutp
    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and <t>LB1.</t> Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by <t>BrUTP</t> incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    Mouse Anti Brutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti brutp/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brutp - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Journal: Nucleus

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    doi: 10.1080/19491034.2015.1004256

    Figure Lengend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Article Snippet: Primary antibodies used were mouse monoclonal anti-LA/C (1:300; JoL2, Chemicon, cat.# MAB3211), rabbit polyclonal anti-LA (1:1000) , rabbit polyclonal anti-LB1 (1:1000) , mouse monoclonal anti-LB2 (1:100; LN43, Abcam, cat.# ab8983), rabbit polyclonal anti-H3K9me3 (1:1000, Abcam, cat.# ab8898) and rabbit polyclonal anti-H3K27me3 (1:1000, Abcam, cat.# ab108245), rabbit polyclonal anti-H3K4me2 (1:1000, Abcam, cat.# ab7766), rabbit polyclonal anti-H3K36me3 (1:1000, Abcam, cat.# ab9050, rabbit polyclonal anti-AcH3 (1:1000, Upstate/Merck Millipore, cat.# 06–599), mouse monoclonal anti-Pol II (1:1000, 4H8, Abcam, cat.# ab5408), mouse monoclonal anti-Pol II phosphorylated at Ser2 (H5, 1:100, Abcam, cat.# ab24758), mouse monoclonal anti-FLAG (1:400, clone M2, Sigma, cat.# F1804), rabbit polyclonal anti-SUN1 and anti-SUN2 (1:40 and 1:20; kindly provided by Didier Hodzic) , goat polyclonal anti-LB1 (1:200 Santa Cruz cat.# sc-6217), mouse anti-BrUTP (1:100, Caltag Laboratories, clone Br-3), rabbit polyclonal anti-p53 (1:100, Imgenex, cat.# IMG-533) and rabbit polyclonal anti-SKIP (NCOA62) (1:100, Abcam, cat.# ab153887).

    Techniques: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization