active caspase 3  (Cell Signaling Technology Inc)

Journal: Viruses

Article Title: Glycoprotein Production by Bursal Secretory Dendritic Cells in Normal, Vaccinated, and Infectious Bursal Disease Virus (IBDV)-Infected Chickens

doi: 10.3390/v14081689

Figure Lengend Snippet: Immunocytochemistry of anti-IBDV; 5A10 mAb, 2 days pi. ( a ) In the very virulent virus infected birds, the majority of 5A10 positive cells are located close to the CMEA(s) and they are able to assemble in the periphery of the cortex. Many individual cells are between the CMEA(s) and periphery of the cortex. Follicles are still found without infected cells. ( b ) The Delaware-E strain shows much fewer infected follicles, but occasionally a heavily infected follicle occurs, which differs from that of very virulent virus infected follicles. Many IBDV-positive cells are in the medulla and the cortex is homogenously covered by IBDV-positive cells. ( c ) The vaccine 228E virus positive cells, seem to be similar to that of the Delaware-E strain. ( d ) Macrophage-like cells (Mal) from a vaccine-strain virus inoculated chicken. The cell has two corpuscles (fused BSDC granules, outlined) and the periphery of the cytoplasm is full of neutral lipid droplets. ( e ) Higher magnification from a Mal. The corpuscles (outlined) are full of virus particles and the dark nodules and myelin—the rest is corpuscles material. ( e′ ) is an inset of ( e ). The rectangle shows higher magnification of the virus particles. ( f ) At 36 h pi, the caspase3 staining shows a highly variable number of apoptotic cells in the follicles. ( g ) Higher magnification shows that the caspase3-positive cells are found in the medulla and a few cells occur in the periphery of the cortex.

Article Snippet: Cleaved (active) Caspase3 mAb (Cell Signaling No9660) was used for identification of apoptotic cells.

Techniques: Immunocytochemistry, Virus, Infection, Staining