mosquito  (SPT Labtech)


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    Structured Review

    SPT Labtech mosquito
    Mosquito, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mosquito/product/SPT Labtech
    Average 86 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    mosquito - by Bioz Stars, 2022-08
    86/100 stars

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    SPT Labtech mosquito lcp
    KEK-style membrane sandwich <t>LCP</t> crystallization. ( a ) A KEK original adaptor (bottom) is in the <t>SBS</t> format and can be used with PXS2 because the adaptor is compatible with the KEK crystallization plate. A film sandwich LCP plate supported by glass plates (top) fits onto the adaptor. ( b ) Schematic drawing of a cross section of the KEK-style LCP plate. ( c ) Comparison of the crystallization-drop images under a polarized condition between glass-supported (KEK-style, left) and plastic-supported (MD Diffrax, right) LCP plates.
    Mosquito Lcp, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mosquito lcp/product/SPT Labtech
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mosquito lcp - by Bioz Stars, 2022-08
    96/100 stars
      Buy from Supplier

    94
    SPT Labtech spt labtech mosquito hv
    Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. ( A ) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and <t>mosquito</t> <t>(SPT</t> <t>Labtech)</t> (Figs. 2 and 5). ( B ) Workflow for performance comparison between SSsc and SS3. CD3 + T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). ( C ) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).
    Spt Labtech Mosquito Hv, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spt labtech mosquito hv/product/SPT Labtech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spt labtech mosquito hv - by Bioz Stars, 2022-08
    94/100 stars
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    KEK-style membrane sandwich LCP crystallization. ( a ) A KEK original adaptor (bottom) is in the SBS format and can be used with PXS2 because the adaptor is compatible with the KEK crystallization plate. A film sandwich LCP plate supported by glass plates (top) fits onto the adaptor. ( b ) Schematic drawing of a cross section of the KEK-style LCP plate. ( c ) Comparison of the crystallization-drop images under a polarized condition between glass-supported (KEK-style, left) and plastic-supported (MD Diffrax, right) LCP plates.

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    Article Title: A fully automated crystallization apparatus for small protein quantities

    doi: 10.1107/S2053230X20015514

    Figure Lengend Snippet: KEK-style membrane sandwich LCP crystallization. ( a ) A KEK original adaptor (bottom) is in the SBS format and can be used with PXS2 because the adaptor is compatible with the KEK crystallization plate. A film sandwich LCP plate supported by glass plates (top) fits onto the adaptor. ( b ) Schematic drawing of a cross section of the KEK-style LCP plate. ( c ) Comparison of the crystallization-drop images under a polarized condition between glass-supported (KEK-style, left) and plastic-supported (MD Diffrax, right) LCP plates.

    Article Snippet: The protein sample is provided in an SBS-formatted 384-well plate which is supplied to the mosquito LCP.

    Techniques: Crystallization Assay

    ( a ) Illustration of the compound-screening workflow. Compound molecules were dissolved in DMSO and introduced into the channel by capillary action. The solvent was then allowed to evaporate at room temperature, leaving the compounds coated onto the inner surface of the channel. Finally, a slurry of crystals was introduced into the coated channel by capillary action. Crystals were prevented from passing out of the channel by notches at the outlet end (see inset). The inlets and outlets were then sealed with epoxy. ( b ) Optical micrograph of a channel in a fabricated chip, showing feature details. The channel was filled with red food coloring for visualization. The scale bar represents 1 mm. ( c ) A top view of a fabricated device with all channels filled with red food coloring. The chip was inserted into a 3D-printed frame, which was mounted on a magnetic base. ( d ) A photograph showing the automated sample filling process performed by a mosquito liquid-handling robot. The chip-inserted frame (on the right side) was placed in a 3D-printed cassette having the same size as a standard well plate. The robot was programmed for automated and precise dispensing of solution (red food coloring shown as an example) onto the channel inlets where capillary action was then used to fill the channel.

    Journal: Journal of Applied Crystallography

    Article Title: A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening

    doi: 10.1107/S1600576721004155

    Figure Lengend Snippet: ( a ) Illustration of the compound-screening workflow. Compound molecules were dissolved in DMSO and introduced into the channel by capillary action. The solvent was then allowed to evaporate at room temperature, leaving the compounds coated onto the inner surface of the channel. Finally, a slurry of crystals was introduced into the coated channel by capillary action. Crystals were prevented from passing out of the channel by notches at the outlet end (see inset). The inlets and outlets were then sealed with epoxy. ( b ) Optical micrograph of a channel in a fabricated chip, showing feature details. The channel was filled with red food coloring for visualization. The scale bar represents 1 mm. ( c ) A top view of a fabricated device with all channels filled with red food coloring. The chip was inserted into a 3D-printed frame, which was mounted on a magnetic base. ( d ) A photograph showing the automated sample filling process performed by a mosquito liquid-handling robot. The chip-inserted frame (on the right side) was placed in a 3D-printed cassette having the same size as a standard well plate. The robot was programmed for automated and precise dispensing of solution (red food coloring shown as an example) onto the channel inlets where capillary action was then used to fill the channel.

    Article Snippet: Sample loading via liquid-handling robot Device channels were prepared using the mosquito liquid-handling robot (SPT Labtech) to deliver 400 nl to the channel inlets.

    Techniques: Chromatin Immunoprecipitation

    Involvement of TM4 in the substrate cleavage and dependence on residue D446 (a) Amino acid sequence alignment of the C-terminal regions of nine RseP orthologues classified as γ -proteobacteria. The UniProtKB accession number of each orthologue is shown (Ec, Escherichia coli ; Kk, Kangiella koreensis ; Se, Salmonella enterica serovar Typhimurium; Yp, Yersinia pestis ; Vc, Vibrio cholerae ; Hi, Haemophilus influenzae ; Pm, Pasteurella multocida ; Pa, Pseudomonas aeruginosa ; Xf, Xylella fastidiosa ). Conserved and similar residues are boxed in black and gray, respectively. The positions of TM segments, structural elements and two featured residues are shown based on those of Ec RseP. (b) Complementation assay. Cultures of E. coli KK31 [Δ rseP /pKK6 (PBAD- rseP )] cells carrying plasmids for Ec RseP (pYH825) or its variants were serially diluted and spotted on L agar plates containing IPTG (left) to test complementation or containing L-arabinose (right) as a control for total-cell count. A representative result from three biological replicates is shown. ΔTM4 and ΔCTail indicate the F426amber and D446amber mutations, respectively. (c) In vivo cleavage assay with long induction. E. coli KA306 (Δ rseA Δ rseP Δ clpP ) cells harboring one plasmid for HA-MBP-RseA148 (pKA65) and one for Ec RseP (pYH825) or its variants were grown at 30°C for 3 h with 1 mM IPTG and 1 mM cAMP in M9-based medium. The cleavage efficiency was determined from immunoblots (images) as in Fig 2e . Bar plots and error bars represent the means ± S.D. from three biological replicates. (d) Conserved residues on TM4. The residues shown as sphere models were mutated to alanine (A442 was mutated to serine) to examine their roles in the proteolytic activity, as shown in Fig. S14. Another mutated residue, R449, was disordered in the electron density map as indicated by dotted lines. The ΔTM4 mutant in (b) lacks the C-terminal residues downstream of F426 (white sphere) while ΔCTail lacks the periplasmic tail region (D446 to the C-terminus). Batimastat and active site residues are shown as stick models. The zinc ion is shown as a sphere. (e) Specific interaction between D446 and PCT-H2. TM4 and the PCT-loop, including PCT-SH and PCT-H2, are shown as stick models in magenta, pink, and salmon, respectively. D446 interacts with the electropositive N-terminal end of the PCT-H2 helix dipole where the side chain of D446 and the main chain N-H group of S363 (each in white) form a hydrogen bond.

    Journal: bioRxiv

    Article Title: Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP

    doi: 10.1101/2022.01.31.478169

    Figure Lengend Snippet: Involvement of TM4 in the substrate cleavage and dependence on residue D446 (a) Amino acid sequence alignment of the C-terminal regions of nine RseP orthologues classified as γ -proteobacteria. The UniProtKB accession number of each orthologue is shown (Ec, Escherichia coli ; Kk, Kangiella koreensis ; Se, Salmonella enterica serovar Typhimurium; Yp, Yersinia pestis ; Vc, Vibrio cholerae ; Hi, Haemophilus influenzae ; Pm, Pasteurella multocida ; Pa, Pseudomonas aeruginosa ; Xf, Xylella fastidiosa ). Conserved and similar residues are boxed in black and gray, respectively. The positions of TM segments, structural elements and two featured residues are shown based on those of Ec RseP. (b) Complementation assay. Cultures of E. coli KK31 [Δ rseP /pKK6 (PBAD- rseP )] cells carrying plasmids for Ec RseP (pYH825) or its variants were serially diluted and spotted on L agar plates containing IPTG (left) to test complementation or containing L-arabinose (right) as a control for total-cell count. A representative result from three biological replicates is shown. ΔTM4 and ΔCTail indicate the F426amber and D446amber mutations, respectively. (c) In vivo cleavage assay with long induction. E. coli KA306 (Δ rseA Δ rseP Δ clpP ) cells harboring one plasmid for HA-MBP-RseA148 (pKA65) and one for Ec RseP (pYH825) or its variants were grown at 30°C for 3 h with 1 mM IPTG and 1 mM cAMP in M9-based medium. The cleavage efficiency was determined from immunoblots (images) as in Fig 2e . Bar plots and error bars represent the means ± S.D. from three biological replicates. (d) Conserved residues on TM4. The residues shown as sphere models were mutated to alanine (A442 was mutated to serine) to examine their roles in the proteolytic activity, as shown in Fig. S14. Another mutated residue, R449, was disordered in the electron density map as indicated by dotted lines. The ΔTM4 mutant in (b) lacks the C-terminal residues downstream of F426 (white sphere) while ΔCTail lacks the periplasmic tail region (D446 to the C-terminus). Batimastat and active site residues are shown as stick models. The zinc ion is shown as a sphere. (e) Specific interaction between D446 and PCT-H2. TM4 and the PCT-loop, including PCT-SH and PCT-H2, are shown as stick models in magenta, pink, and salmon, respectively. D446 interacts with the electropositive N-terminal end of the PCT-H2 helix dipole where the side chain of D446 and the main chain N-H group of S363 (each in white) form a hydrogen bond.

    Article Snippet: 50 or 100 nL aliquots of the protein-monoolein mixture were dispensed onto 96-well glass plates and overlaid with 800 nL of crystallization solution using a mosquito LCP (TTP LabTech) or Crystal Gryphon LCP (Art Robbins Instruments).

    Techniques: Sequencing, Cell Counting, In Vivo, Cleavage Assay, Plasmid Preparation, Western Blot, Activity Assay, Mutagenesis

    Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. ( A ) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). ( B ) Workflow for performance comparison between SSsc and SS3. CD3 + T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). ( C ) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Benchmarking Single-Cell mRNA–Sequencing Technologies Uncovers Differences in Sensitivity and Reproducibility in Cell Types With Low RNA Content

    doi: 10.7171/3fc1f5fe.dbeabb2a

    Figure Lengend Snippet: Testing overview for SSsc and SSlp kits. For all testing schema, sample preparation was followed by library preparation, Illumina sequencing, and analysis using Cogent AP software. ( A ) Workflow for testing cultured cells (GM12878 and CHO cells) isolated by Fluorescence-activated cell sorting (FACS). This workflow was used for a performance comparison between SSsc and SS2 and for verifying compatibility with miniaturized volumes on the MANTIS (Formulatrix) and mosquito (SPT Labtech) (Figs. 2 and 5). ( B ) Workflow for performance comparison between SSsc and SS3. CD3 + T cells were isolated from human PBMCs by FACS, and the appropriate user manual or protocol was followed for RNA isolation (Fig. 3). ( C ) Workflow for performance comparison between SSsc PLUS (SSsc + SSlp) and Nextera XT using isolated RNA (control mouse brain RNA; Fig. 4).

    Article Snippet: The Formulatrix MANTIS and SPT Labtech mosquito HV, 2 of the most commonly used liquid handlers in the single-cell RNA-seq community, were used to compare SSsc’s sensitivity at full volume (FV) versus the commonly used miniaturized volume for each instrument: quarter volume (Quarter) on the MANTIS and one-eighth volume (Eighth) on the mosquito HV.

    Techniques: Sample Prep, Sequencing, Software, Cell Culture, Isolation, Fluorescence, FACS, Single-particle Tracking