anti mor polyclonal antibody  (Novus Biologicals)

Journal: Journal of Pain Research

Article Title: GPER Enhances Chronic Burn Pain via PKC-Mediated Mu-Opioid Receptor Phosphorylation in the Rostral Ventromedial Medulla

doi: 10.2147/JPR.S529103

Figure Lengend Snippet: Alterations in MOR protein expression, phosphorylation, neuronal positivity, and membrane translocation across heterogeneous pain manifestations. ( A ) Representative WB bands illustrating the expression levels of MOR and p-MOR of mice subjected to different pain manifestations (MW: p-MOR, 75 kDa; MOR, 55 kDa; β-Actin, 42 kDa). ( B, C ) Quantitative analysis of p-MOR and MOR expression in the RVM of mice experiencing distinct pain phenotypes (Naïve, Pain persisting, and Pain recovery cohort, n = 6). ( D ) Representative immunofluorescence images depicting the distribution of MOR + neurons within the RVM. All scale bars, 100 μm. ( E ) Statistical analysis of MOR+ neuron counts in the RVM across heterogeneous pain phenotypes (n = 6). ( F and G ) Membrane translocation of MOR protein in mice subjected to heterogeneous pain conditions (n = 6). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After blocking with either 5% BSA or 5% nonfat dried milk for 2 h, the membranes were incubated overnight with specific primary antibodies against GPER (1:1000, Novus), MOR (1:1000, Santa Cruz Biotechnology), p-MOR (1:1000, Signalway Antibody), PKC α (1:1000, Abcam), PKC ε (1:1000, Proteintech), p-PKC (1:1000, Abcam), GRK2 (1:2000, Immunoway), GRK3 (1:2000, Immunoway), and β-actin (1:1000, Cell Signaling Technology).

Techniques: Expressing, Phospho-proteomics, Membrane, Translocation Assay, Immunofluorescence

Journal: Journal of Pain Research

Article Title: GPER Enhances Chronic Burn Pain via PKC-Mediated Mu-Opioid Receptor Phosphorylation in the Rostral Ventromedial Medulla

doi: 10.2147/JPR.S529103

Figure Lengend Snippet: Quantification of activated GPER-positive and PKC-positive neurons in the RVM of the brain across heterogeneous pain conditions. ( A ) Representative immunofluorescence images illustrating the distribution of GPER-positive (GPER + ) neurons, MOR + neurons, PKCα-positive (PKCα + ) neurons, and PKCε-positive (PKCε + ) neurons in the RVM of mice subjected to divergent pain conditions on day 20 post-incision surgery. (a) Green: GPER + neurons; Red: MOR + neurons. (b) Green: GPER + neurons; Red: PKCα + neurons. (c) Green: GPER+ neurons; Red: PKCε + neurons. All scale bars, 100 μm or 25 μm. ( B – E ) Quantitative analysis of GPER+, PKCα+, and PKCε+ neuronal counts in the RVM under heterogeneous pain conditions (n = 6). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After blocking with either 5% BSA or 5% nonfat dried milk for 2 h, the membranes were incubated overnight with specific primary antibodies against GPER (1:1000, Novus), MOR (1:1000, Santa Cruz Biotechnology), p-MOR (1:1000, Signalway Antibody), PKC α (1:1000, Abcam), PKC ε (1:1000, Proteintech), p-PKC (1:1000, Abcam), GRK2 (1:2000, Immunoway), GRK3 (1:2000, Immunoway), and β-actin (1:1000, Cell Signaling Technology).

Techniques: Immunofluorescence

Journal: Journal of Pain Research

Article Title: GPER Enhances Chronic Burn Pain via PKC-Mediated Mu-Opioid Receptor Phosphorylation in the Rostral Ventromedial Medulla

doi: 10.2147/JPR.S529103

Figure Lengend Snippet: Modulation of MOR phosphorylation by PKC across different experimental cohorts. ( A and B ) PWT and PWL were assessed in mice following microinjection of G1, STS, or co-administration of G1 and STS for pain recovery (n = 10, comparison between pain recovery + G1 cohort and pain recovery + STS + G1 cohort). ( C ) Representative WB bands illustrating p-MOR expression in the RVM across different experimental cohorts. ( D ) Quantitative analysis of MOR phosphorylation level across different cohorts (Pain recovery + DMSO, Pain recovery + STS, Pain recovery + G1, and Pain recovery + STS + G1, n = 6). ( E and F ) PWT and PWL were measured in pain persisting mice following microinjection of STS (n = 10). ( G ) Representative WB bands depicting p-MOR expression in the RVM of pain persisting + DMSO and pain persisting + STS cohort. ( H ) Quantitative analysis of p-MOR expression in the RVM across cohorts (n = 6). ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: After blocking with either 5% BSA or 5% nonfat dried milk for 2 h, the membranes were incubated overnight with specific primary antibodies against GPER (1:1000, Novus), MOR (1:1000, Santa Cruz Biotechnology), p-MOR (1:1000, Signalway Antibody), PKC α (1:1000, Abcam), PKC ε (1:1000, Proteintech), p-PKC (1:1000, Abcam), GRK2 (1:2000, Immunoway), GRK3 (1:2000, Immunoway), and β-actin (1:1000, Cell Signaling Technology).

Techniques: Phospho-proteomics, Microinjection, Comparison, Expressing