mononuclear cells mncs  (Lonza)


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    Name:
    Human Bone Marrow Mononuclear Cells MNCs Cryopreserved Cells
    Description:
    Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing 25 million cells
    Catalog Number:
    2m-125c
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza mononuclear cells mncs
    Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing 25 million cells
    https://www.bioz.com/result/mononuclear cells mncs/product/Lonza
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Integrins α2β1 and α11β1 regulate the survival of mesenchymal stem cells on collagen I
    Article Snippet: .. CD271-negative mononuclear cells (MNCs) and CD271-positive hMSC were isolated with a CD271 MicroBead kit (Miltenyi Biotec, Bergish Gladbach, Germany) from three human bone marrow MNC fractions (Cat. No. 2M-125C) purchased from Lonza and immediately after the cell separation the freshly isolated cells were lysed for protein analysis. .. shRNA cloning, lentiviral production and infection of hMSC shRNA oligonucleotides were designed with Invitrogen's BLOCK-iT RNAi Designer (Invitrogen) against the human integrin α 1, α 2 and α 11 and β -galactosidase genes ( ).

    Article Title: Enrichment of the tumour immune microenvironment in patients with desmoplastic colorectal liver metastasis
    Article Snippet: .. Data on (part of) this cohort has previously been published., , The relative proportions of CD4+ T cells, CD4+FoxP3− T helper cells, CD4+FoxP3+ T regulatory cells, and CD8+ cytotoxic T cells within live CD3+ T cells were determined by flow cytometry in mononuclear cells (MNCs) isolated from fresh tumour tissue, tumour-free liver (obtained as distant as possibly from the tumour; minimum 1 cm distance), and in peripheral blood mononuclear cells (PMBCs) isolated from peripheral blood collected prior to surgery. .. Ficoll density gradient centrifugation was used for PBMC isolation.

    Flow Cytometry:

    Article Title: Enrichment of the tumour immune microenvironment in patients with desmoplastic colorectal liver metastasis
    Article Snippet: .. Data on (part of) this cohort has previously been published., , The relative proportions of CD4+ T cells, CD4+FoxP3− T helper cells, CD4+FoxP3+ T regulatory cells, and CD8+ cytotoxic T cells within live CD3+ T cells were determined by flow cytometry in mononuclear cells (MNCs) isolated from fresh tumour tissue, tumour-free liver (obtained as distant as possibly from the tumour; minimum 1 cm distance), and in peripheral blood mononuclear cells (PMBCs) isolated from peripheral blood collected prior to surgery. .. Ficoll density gradient centrifugation was used for PBMC isolation.

    Gradient Centrifugation:

    Article Title: Bone marrow-derived from the human femoral shaft as a new source of mesenchymal stem/stromal cells: an alternative cell material for banking and clinical transplantation
    Article Snippet: .. After density gradient centrifugation, mononuclear cells (MNC) were retrieved from the buffy coat layer by pipetting and washed twice with PBS. .. The final product was re-suspended in MSC culture medium (Lonza) and seeded at high density (2 × 105 /cm2 ) on culture dishes.

    Derivative Assay:

    Article Title: Impact of age on the efficacy of bone marrow mononuclear cell transplantation in experimental stroke
    Article Snippet: .. BM MNC preparation Cryopreserved human bone marrow derived mononuclear cells (BM MNC) from young donors (n = 4; 24 ± 4 years) were obtained from Lonza (Walkersville, USA). .. BM MNCs have been isolated by Ficoll-Paque density gradient centrifugation according to the manufacturer’s instructions.

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  • 86
    Lonza cd8 t cells pbmcs
    IWP-2 does not inhibit Wnt production from primary human CD8+ T cells. CD8+ T cells were isolated from <t>PBMCs</t> from healthy donors by negative selection and subsequently activated with 1 μg each anti-CD3/anti-CD28 then propagated in presence of 100 units/ml IL-2 for three days. On the third day supernatant was collected and 25 μl was analyzed by western blot for presence of Wnt 1 (A), 3 (B), 6 (C), 7a (D), 10a (E), and 16a (F).
    Cd8 T Cells Pbmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd8 t cells pbmcs/product/Lonza
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    Lonza glycolipid pulsed autologous pbmcs
    Cytokine levels in supernatant of co-culture of human <t>iNKT</t> cells and glycolipid-loaded irradiated <t>PBMC</t>
    Glycolipid Pulsed Autologous Pbmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glycolipid pulsed autologous pbmcs/product/Lonza
    Average 85 stars, based on 1 article reviews
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    pbmc  (Lonza)
    93
    Lonza pbmc
    Functional analysis of MAIT cells in individuals of different ages. <t>PBMC</t> or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. <t>ICS</t> was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.
    Pbmc, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/Lonza
    Average 93 stars, based on 168 article reviews
    Price from $9.99 to $1999.99
    pbmc - by Bioz Stars, 2020-09
    93/100 stars
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    Image Search Results


    IWP-2 does not inhibit Wnt production from primary human CD8+ T cells. CD8+ T cells were isolated from PBMCs from healthy donors by negative selection and subsequently activated with 1 μg each anti-CD3/anti-CD28 then propagated in presence of 100 units/ml IL-2 for three days. On the third day supernatant was collected and 25 μl was analyzed by western blot for presence of Wnt 1 (A), 3 (B), 6 (C), 7a (D), 10a (E), and 16a (F).

    Journal: PLoS ONE

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells

    doi: 10.1371/journal.pone.0092159

    Figure Lengend Snippet: IWP-2 does not inhibit Wnt production from primary human CD8+ T cells. CD8+ T cells were isolated from PBMCs from healthy donors by negative selection and subsequently activated with 1 μg each anti-CD3/anti-CD28 then propagated in presence of 100 units/ml IL-2 for three days. On the third day supernatant was collected and 25 μl was analyzed by western blot for presence of Wnt 1 (A), 3 (B), 6 (C), 7a (D), 10a (E), and 16a (F).

    Article Snippet: Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation.

    Techniques: Isolation, Selection, Western Blot

    Cytokine levels in supernatant of co-culture of human iNKT cells and glycolipid-loaded irradiated PBMC

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Enhanced TCR footprint by a novel glycolipid increases NKT dependent tumor protection

    doi: 10.4049/jimmunol.1203134

    Figure Lengend Snippet: Cytokine levels in supernatant of co-culture of human iNKT cells and glycolipid-loaded irradiated PBMC

    Article Snippet: Subsequently, 5×104 iNKT cells were stimulated with 105 glycolipid pulsed autologous PBMCs in RPMI 1640 media supplemented with 10% human AB serum (Lonza), 1% sodium pyruvate, 1% nonessential amino acids and 1% penicillin/streptomycin (all from Invitrogen).

    Techniques: Co-Culture Assay, Irradiation

    Functional analysis of MAIT cells in individuals of different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.

    Journal: bioRxiv

    Article Title: Postnatal Expansion, Maturation and Functionality of MR1T Cells in Humans

    doi: 10.1101/2019.12.20.882746

    Figure Lengend Snippet: Functional analysis of MAIT cells in individuals of different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells or uninfected A549 cells and then stained with the MR1-5-OP-RU or MR1/6FP tetramers, followed by staining with a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. A . Dot plots showing representative co-staining of live, TCRγδ - CD3 + TRAV1-2 + CD26 + CD161 + cells with MR1-5-OP-RU and TNF in M. smegmatis stimulated or unstimulated samples. The gating strategy is shown in Figure S3. Examples of the TNF response in a neonate, infant and adult are shown. B . Frequencies of functional, phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + or MR1-5-OP-RU - as a proportion of CD3 + TCRγδ - T cells. Horizontal lines depict the median and the error bars the 95% confidence interval. Wilcoxon-rank sum was used to test differences within the same cohort.

    Article Snippet: Flow cytometry assays To perform ICS, cryopreserved PBMC were thawed in the presence of DNAse, resuspended, in 10% heat inactivated human serum with RPMI (Lonza) at a concentration of 2 × 10^7/ml.

    Techniques: Functional Assay, Incubation, Infection, Staining

    Phenotypic analysis of functional MR1T cells at different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells, uninfected A549 cells, or uninfected A549 cells and PMA/ionomycin. All cells were then stained with the MR1-5-OP-RU or MR1-6FP tetramers, followed by a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. Live, TCRγδ - CD3 + MR1-5-OP-RU + cells were gated and the TNF + percentage determined in both the M. smegmatis stimulated and unstimulated conditions. The gating strategy is shown in Figure S3. A . Dot plots showing TNF expression by live, TCRγδ - MR1-5-OP-RU + CD3 + cells in the same representative neonate, infant and adult as Figure 2A . B . Background subtracted frequencies of TNF + MR1-5-OP-RU + CD3 + cells in response to M. smegmatis -infected A549 cells as a percentage of total MR1/5-OP-RU + CD3 + cells are shown. Mann-Whitney u-tests were used to test differences between groups. C . Background subtracted frequencies of TNF + cells to PMA/ionomycin among different T cell subpopulations, 1) MR1T cells (CD3 + TCRγδ - MR1-5-OP-RU + cells); 2) γδ T cells (CD3 + TCRγδ + MR1-5-OP-RU - cells); 3) CD8 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD8 + cells); and 4) CD4 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD4 + cells). Wilcoxon-rank sum was used to test differences within the same cohort. D . and E . Frequencies of TNF + TRAV1-2 + MR1-5-OP-RU + CD3 + and TNF + TRAV1-2 - MR1-5-OP-RU + CD3 + cells in response to M. smegmatis infected A549 cells ( D ) or PMA/ionomycin stimulation ( E ) minus the background frequencies of TNF + TRAV1-2 + /- MR1-5-OP-RU + CD3 + cells. Wilcoxon-rank sum was used to test differences within the same cohort.

    Journal: bioRxiv

    Article Title: Postnatal Expansion, Maturation and Functionality of MR1T Cells in Humans

    doi: 10.1101/2019.12.20.882746

    Figure Lengend Snippet: Phenotypic analysis of functional MR1T cells at different ages. PBMC or CBMC from the US cohort were incubated overnight with M. smegmatis -infected A549 cells, uninfected A549 cells, or uninfected A549 cells and PMA/ionomycin. All cells were then stained with the MR1-5-OP-RU or MR1-6FP tetramers, followed by a live/dead discriminator and antibodies to TCRγδ, CD3, CD4, CD8, TRAV1-2, CD26 and CD161. ICS was then performed and the cells stained for TNF. Live, TCRγδ - CD3 + MR1-5-OP-RU + cells were gated and the TNF + percentage determined in both the M. smegmatis stimulated and unstimulated conditions. The gating strategy is shown in Figure S3. A . Dot plots showing TNF expression by live, TCRγδ - MR1-5-OP-RU + CD3 + cells in the same representative neonate, infant and adult as Figure 2A . B . Background subtracted frequencies of TNF + MR1-5-OP-RU + CD3 + cells in response to M. smegmatis -infected A549 cells as a percentage of total MR1/5-OP-RU + CD3 + cells are shown. Mann-Whitney u-tests were used to test differences between groups. C . Background subtracted frequencies of TNF + cells to PMA/ionomycin among different T cell subpopulations, 1) MR1T cells (CD3 + TCRγδ - MR1-5-OP-RU + cells); 2) γδ T cells (CD3 + TCRγδ + MR1-5-OP-RU - cells); 3) CD8 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD8 + cells); and 4) CD4 + T cells (CD3 + TCRγδ - MR1-5-OP-RU - CD4 + cells). Wilcoxon-rank sum was used to test differences within the same cohort. D . and E . Frequencies of TNF + TRAV1-2 + MR1-5-OP-RU + CD3 + and TNF + TRAV1-2 - MR1-5-OP-RU + CD3 + cells in response to M. smegmatis infected A549 cells ( D ) or PMA/ionomycin stimulation ( E ) minus the background frequencies of TNF + TRAV1-2 + /- MR1-5-OP-RU + CD3 + cells. Wilcoxon-rank sum was used to test differences within the same cohort.

    Article Snippet: Flow cytometry assays To perform ICS, cryopreserved PBMC were thawed in the presence of DNAse, resuspended, in 10% heat inactivated human serum with RPMI (Lonza) at a concentration of 2 × 10^7/ml.

    Techniques: Functional Assay, Incubation, Infection, Staining, Expressing, MANN-WHITNEY

    Frequencies of tetramer-defined MR1T cell and phenotypically defined MAIT cell populations in peripheral blood from individuals of different ages. PBMC or CBMC were stained with a live/dead discriminator, antibodies to CD3, CD4, CD8, TRAV1-2, CD26, CD161, and either the MR1-5-OP-RU or MR1-6FP tetramers. Live, CD3 + lymphocytes were gated and the frequencies of MR1-5-OP-RU + or TRAV1-2 + CD26 + CD161 + cells as a percentage of CD3 + lymphocytes were determined (gating strategy in Figure S2). A . Flow cytometry plots showing CD3 + T cell staining with MR1-5-OP-RU tetramer, MR1-6FP tetramer, TRAV1-2 or CD26/CD161 of samples from a representative US adult, infant and neonate. B . Frequencies of tetramer-defined (CD3 + MR1-5-OP-RU + ) and phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells in neonates, 10 week-old infants and adolescents from South Africa, neonates, 12 month-old infants and adults from the United States and infants (0-2 years old), children (2-5 years old) and adults from Uganda (all cohorts, n =10). Mann-Whitney u-tests were used to test differences between groups. Horizontal lines depict the median and the error bars the 95% confidence interval. C . Relative proportions of median phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + (Blue) and MR1-5-OP-RU - (Red shades) for each age group at each site. In each doughnut chart the MR1-5-OP-RU - population is further divided into CD8 + , CD8 + CD4 + , CD4 + , and CD8 - CD4 - populations.

    Journal: bioRxiv

    Article Title: Postnatal Expansion, Maturation and Functionality of MR1T Cells in Humans

    doi: 10.1101/2019.12.20.882746

    Figure Lengend Snippet: Frequencies of tetramer-defined MR1T cell and phenotypically defined MAIT cell populations in peripheral blood from individuals of different ages. PBMC or CBMC were stained with a live/dead discriminator, antibodies to CD3, CD4, CD8, TRAV1-2, CD26, CD161, and either the MR1-5-OP-RU or MR1-6FP tetramers. Live, CD3 + lymphocytes were gated and the frequencies of MR1-5-OP-RU + or TRAV1-2 + CD26 + CD161 + cells as a percentage of CD3 + lymphocytes were determined (gating strategy in Figure S2). A . Flow cytometry plots showing CD3 + T cell staining with MR1-5-OP-RU tetramer, MR1-6FP tetramer, TRAV1-2 or CD26/CD161 of samples from a representative US adult, infant and neonate. B . Frequencies of tetramer-defined (CD3 + MR1-5-OP-RU + ) and phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells in neonates, 10 week-old infants and adolescents from South Africa, neonates, 12 month-old infants and adults from the United States and infants (0-2 years old), children (2-5 years old) and adults from Uganda (all cohorts, n =10). Mann-Whitney u-tests were used to test differences between groups. Horizontal lines depict the median and the error bars the 95% confidence interval. C . Relative proportions of median phenotypically-defined (CD3 + TRAV1-2 + CD26 + CD161 + ) MAIT cells that are MR1-5-OP-RU + (Blue) and MR1-5-OP-RU - (Red shades) for each age group at each site. In each doughnut chart the MR1-5-OP-RU - population is further divided into CD8 + , CD8 + CD4 + , CD4 + , and CD8 - CD4 - populations.

    Article Snippet: Flow cytometry assays To perform ICS, cryopreserved PBMC were thawed in the presence of DNAse, resuspended, in 10% heat inactivated human serum with RPMI (Lonza) at a concentration of 2 × 10^7/ml.

    Techniques: Staining, Flow Cytometry, MANN-WHITNEY

    EHV-4 downregulates cell surface adhesion molecules on peripheral blood mononuclear cells (PBMC). ( A ) surface expression of very late antigen-4 (VLA-4) and lymphocyte function-associated antigen-1 (LFA-1) on the surface of mock-infected PBMC and virus infection ( black line: EHV-1; dotted line: EHV-4) levels of PBMC were determined by flow cytometry. ( B and C ) Equine PBMC were either mock-infected or infected with EHV-4 or EHV-1 and subjected to flow cytometric analysis. Histograms and bars of VLA-4 ( B ) and LFA-1 ( C ) expression after detection with the relevant antibodies are shown. Viable cells (10,000) were analyzed for each sample. Grey filled: mock-infected cells; dotted line: mock-infected cells with the relevant antibody; black line: EHV-4-infected cells; grey line: EHV-1-infected cells. For the bars, the expression level of adhesion molecules (VLA-4 or LFA-1) was set to 100%. All data represent the mean ± SD of three independent experiments. A significant downregulation (one-way ANOVA; p

    Journal: Viruses

    Article Title: The Role of the Equine Herpesvirus Type 1 (EHV-1) US3-Encoded Protein Kinase in Actin Reorganization and Nuclear Egress

    doi: 10.3390/v8100275

    Figure Lengend Snippet: EHV-4 downregulates cell surface adhesion molecules on peripheral blood mononuclear cells (PBMC). ( A ) surface expression of very late antigen-4 (VLA-4) and lymphocyte function-associated antigen-1 (LFA-1) on the surface of mock-infected PBMC and virus infection ( black line: EHV-1; dotted line: EHV-4) levels of PBMC were determined by flow cytometry. ( B and C ) Equine PBMC were either mock-infected or infected with EHV-4 or EHV-1 and subjected to flow cytometric analysis. Histograms and bars of VLA-4 ( B ) and LFA-1 ( C ) expression after detection with the relevant antibodies are shown. Viable cells (10,000) were analyzed for each sample. Grey filled: mock-infected cells; dotted line: mock-infected cells with the relevant antibody; black line: EHV-4-infected cells; grey line: EHV-1-infected cells. For the bars, the expression level of adhesion molecules (VLA-4 or LFA-1) was set to 100%. All data represent the mean ± SD of three independent experiments. A significant downregulation (one-way ANOVA; p

    Article Snippet: PBMCs were infected with either parental (EHV-1 or EHV-4) or mutant (EHV-1∆US3) viruses (MOI = 1) or transfected with different expression vectors (pcDNA3, pcDNA3-US3_1 or pcDNA3-US3_4; transfection efficiency was around 40%) using Nucleofector™ (Lonza, Köln, Germany) [ ].

    Techniques: Expressing, Infection, Flow Cytometry, Cytometry