il 17f  (R&D Systems)

 
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    Name:
    Human IL 17F Fluorescein conjugated Antibody
    Description:
    The Human IL 17F Fluorescein conjugated Antibody from R D Systems is a mouse monoclonal antibody to IL 17F This antibody reacts with human The Human IL 17F Fluorescein conjugated Antibody has been validated for the following applications Intracellular Staining by Flow Cytometry
    Catalog Number:
    ic13351f-025
    Price:
    129
    Applications:
    Intracellular Staining by Flow Cytometry
    Host:
    Mouse
    Purity:
    Protein A or G purified from hybridoma culture supernatant
    Conjugate:
    Fluorescein
    Immunogen:
    E. coli-derived recombinant human IL-17F, Arg31-Gln163, Accession # Q96PD4
    Size:
    25 Tests
    Category:
    Primary Antibodies
    Isotype:
    IgG2b
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    Structured Review

    R&D Systems il 17f
    Human IL 17F Fluorescein conjugated Antibody
    The Human IL 17F Fluorescein conjugated Antibody from R D Systems is a mouse monoclonal antibody to IL 17F This antibody reacts with human The Human IL 17F Fluorescein conjugated Antibody has been validated for the following applications Intracellular Staining by Flow Cytometry
    https://www.bioz.com/result/il 17f/product/R&D Systems
    Average 94 stars, based on 7178 article reviews
    Price from $9.99 to $1999.99
    il 17f - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "IL-1?-INDUCED POST-TRANSITION EFFECT OF NF-KAPPAB PROVIDES TIME-DEPENDENT WAVE OF SIGNALS FOR INITIAL PHASE OF INTRAPOSTATIC INFLAMMATION"

    Article Title: IL-1?-INDUCED POST-TRANSITION EFFECT OF NF-KAPPAB PROVIDES TIME-DEPENDENT WAVE OF SIGNALS FOR INITIAL PHASE OF INTRAPOSTATIC INFLAMMATION

    Journal:

    doi: 10.1002/pros.20916

    Immunostaining for chemoattractant receptor CXCR1/IL-8RA and IL-17F in representative samples of prostates from C57BL/6J mice. IL-1 β (10 μg/kg) was i.v. injected in the tail vein. To proof pro-inflammatory ability of IL-1 β along
    Figure Legend Snippet: Immunostaining for chemoattractant receptor CXCR1/IL-8RA and IL-17F in representative samples of prostates from C57BL/6J mice. IL-1 β (10 μg/kg) was i.v. injected in the tail vein. To proof pro-inflammatory ability of IL-1 β along

    Techniques Used: Immunostaining, Mouse Assay, Injection

    Systemic administration of IL-1β induces multitype time-dependent behavior of intraprostatic chemoattractant receptors and IL-17F
    Figure Legend Snippet: Systemic administration of IL-1β induces multitype time-dependent behavior of intraprostatic chemoattractant receptors and IL-17F

    Techniques Used:

    2) Product Images from "IL-1?-INDUCED POST-TRANSITION EFFECT OF NF-KAPPAB PROVIDES TIME-DEPENDENT WAVE OF SIGNALS FOR INITIAL PHASE OF INTRAPOSTATIC INFLAMMATION"

    Article Title: IL-1?-INDUCED POST-TRANSITION EFFECT OF NF-KAPPAB PROVIDES TIME-DEPENDENT WAVE OF SIGNALS FOR INITIAL PHASE OF INTRAPOSTATIC INFLAMMATION

    Journal:

    doi: 10.1002/pros.20916

    Immunostaining for chemoattractant receptor CXCR4 and adhesion molecules (VCAM1) in representative samples of prostates from C57BL/6J mice. IL-1β (10 μg/kg) was i.v. injected in the tail vein. To proof pro-inflammatory ability of IL-1β
    Figure Legend Snippet: Immunostaining for chemoattractant receptor CXCR4 and adhesion molecules (VCAM1) in representative samples of prostates from C57BL/6J mice. IL-1β (10 μg/kg) was i.v. injected in the tail vein. To proof pro-inflammatory ability of IL-1β

    Techniques Used: Immunostaining, Mouse Assay, Injection

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inhibition of Rho-Kinase Downregulates Th17 Cells and Ameliorates Hepatic Fibrosis by Schistosoma japonicum Infection
    Article Snippet: .. IL-4 and IFN-γ were measured with OptEIA kits (BD Bioscience, San Jose, CA, USA); IL-17, IL-17F, and IL-21 were measured with DuoSet ELISA kits (R & D Systems, Minneapolis, MN, USA). .. ELISA plates were developed with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (BD Bioscience, San Jose, CA, USA) and read with a microplate reader.

    Staining:

    Article Title: Immunologic and MRI markers of the therapeutic effect of IFN-β-1a in relapsing-remitting MS
    Article Snippet: .. Cells were fixed, permeabilized, and stained with fluorescein-conjugated antibodies against IFN-γ, IL-4, IL-17A, IL-21, IL-22 (eBioscience), IL-17F, BDNF (R & D Systems, Minneapolis, MN), IL-10, CD4, and CD8 (BD Biosciences, San Jose, CA). .. For NGF staining, cells were stained with primary antibody against NGF (Epitomics, Burlingame, CA), followed by fluorescein isothiocyanate–conjugated secondary antibody (BD Biosciences).

    Article Title: Expression of the T Helper 17-Associated Cytokines IL-17A and IL-17F in Asthma and COPD
    Article Snippet: .. IL-17A and IL-17F Expression in Endobronchial Biopsies Two-micrometer sections were cut and stained using polyclonal antibodies against IL-17A and IL-17F (R & D Systems Europe Ltd), monoclonal antibodies against eosinophils (major basic protein), neutrophils (neutrophil elastase), and mast cells (tryptase), with appropriate isotype controls mouse IgG1 (Dako UK Ltd; Cambridge, England) and goat immunoglobulins (R & D Systems Europe Ltd). .. The number of positive nucleated cells was enumerated per mm2 of bronchial submucosa by a blinded observer as described previously., In a subgroup of six subjects with asthma, IL-17A and IL-17F were colocalized with neutrophils and eosinophils using sequential sections as previously described.

    Article Title: Heterogeneous expression pattern of interleukin 17A (IL-17A), IL-17F and their receptors in synovium of rheumatoid arthritis, psoriatic arthritis and osteoarthritis: possible explanation for nonresponse to anti-IL-17 therapy?
    Article Snippet: .. Antibodies To investigate the detailed expression pattern of IL-17 in synovium, tissue sections were stained using mouse monoclonal antibodies against IL-17A (immunoglobulin G1 (IgG1), clone 41802), IL-17F (IgG2a, clone 197315) and their receptors IL-17RA (IgG1, clone 133617) and IL-17RC (IgG2b, clone 309882), all from R & D Systems (Minneapolis, MN, USA). .. For colocalisation studies, we used antibodies against the transcription factor RORγt (RORc, mouse IgG2a; RayBiotech, Norcross, GA, USA), T cells (biotin-conjugated mouse anti-CD4: IgG1, clone RPA-T4; Biolegend, San Diego, CA, USA; and biotin-conjugated mouse anti-CD8: IgG1, clone RFT8; SouthernBiotech, Birmingham, AL), B cells (biotin-conjugated mouse anti-CD19; IgG1, clone HIB19; Biolegend), macrophages (biotin-conjugated mouse anti-CD68; IgG2b clone Y1/82A; Biolegend; and biotin-conjugated mouse anti-CD163; IgG1 clone GHI/61; Biolegend), neutrophils (biotin-conjugated mouse anti-CD15; IgM clone HI98; eBioscience, San Diego, CA, USA), mast cells (mouse anti–mast cell tryptase (MCT); IgG1 clone AA1; Dako, Glostrup, Denmark), lymphatic vessels (goat polyclonal anti-lymphatic vessel endothelial hyaluronan receptor 1 (Lyve-1); R & D Systems), blood vessels (mouse anti–von Willebrand factor (vWF); IgG1 clone F8/86; Dako) and high endothelial venules (mouse anti–peripheral lymph node addressin (PNAd); IgM clone MECA-79; Biolegend).

    other:

    Article Title: Antibody blockade of IL-17 family cytokines in immunity to acute murine oral mucosal candidiasis
    Article Snippet: These data confirmed that anti-IL-17A antibodies neutralized IL-17A and IL-17AF, as reported [ ], whereas anti-IL-17F antibodies only neutralized IL-17F.

    Expressing:

    Article Title: Expression of the T Helper 17-Associated Cytokines IL-17A and IL-17F in Asthma and COPD
    Article Snippet: .. IL-17A and IL-17F Expression in Endobronchial Biopsies Two-micrometer sections were cut and stained using polyclonal antibodies against IL-17A and IL-17F (R & D Systems Europe Ltd), monoclonal antibodies against eosinophils (major basic protein), neutrophils (neutrophil elastase), and mast cells (tryptase), with appropriate isotype controls mouse IgG1 (Dako UK Ltd; Cambridge, England) and goat immunoglobulins (R & D Systems Europe Ltd). .. The number of positive nucleated cells was enumerated per mm2 of bronchial submucosa by a blinded observer as described previously., In a subgroup of six subjects with asthma, IL-17A and IL-17F were colocalized with neutrophils and eosinophils using sequential sections as previously described.

    Article Title: Heterogeneous expression pattern of interleukin 17A (IL-17A), IL-17F and their receptors in synovium of rheumatoid arthritis, psoriatic arthritis and osteoarthritis: possible explanation for nonresponse to anti-IL-17 therapy?
    Article Snippet: .. Antibodies To investigate the detailed expression pattern of IL-17 in synovium, tissue sections were stained using mouse monoclonal antibodies against IL-17A (immunoglobulin G1 (IgG1), clone 41802), IL-17F (IgG2a, clone 197315) and their receptors IL-17RA (IgG1, clone 133617) and IL-17RC (IgG2b, clone 309882), all from R & D Systems (Minneapolis, MN, USA). .. For colocalisation studies, we used antibodies against the transcription factor RORγt (RORc, mouse IgG2a; RayBiotech, Norcross, GA, USA), T cells (biotin-conjugated mouse anti-CD4: IgG1, clone RPA-T4; Biolegend, San Diego, CA, USA; and biotin-conjugated mouse anti-CD8: IgG1, clone RFT8; SouthernBiotech, Birmingham, AL), B cells (biotin-conjugated mouse anti-CD19; IgG1, clone HIB19; Biolegend), macrophages (biotin-conjugated mouse anti-CD68; IgG2b clone Y1/82A; Biolegend; and biotin-conjugated mouse anti-CD163; IgG1 clone GHI/61; Biolegend), neutrophils (biotin-conjugated mouse anti-CD15; IgM clone HI98; eBioscience, San Diego, CA, USA), mast cells (mouse anti–mast cell tryptase (MCT); IgG1 clone AA1; Dako, Glostrup, Denmark), lymphatic vessels (goat polyclonal anti-lymphatic vessel endothelial hyaluronan receptor 1 (Lyve-1); R & D Systems), blood vessels (mouse anti–von Willebrand factor (vWF); IgG1 clone F8/86; Dako) and high endothelial venules (mouse anti–peripheral lymph node addressin (PNAd); IgM clone MECA-79; Biolegend).

    Blocking Assay:

    Article Title: Th17 cytokines differentiate obesity from obesity-associated type 2 diabetes and promote TNFα production
    Article Snippet: .. Blocking Antibody Studies Neutralizing antibodies specific to IL-17A (100 ng-1µg) and IL-17F (1 µg) (R & D Systems or LifeSpan BioSciences) or IgG isotype controls (0.1–20µg) were added at the same time as stimuli. .. Flow Cytometry Stimulated PBMCs were cultured with Brefeldin A (3µg/ml; eBioscience) added for the last 4hrs.

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    R&D Systems mononuclear cells mnc cd45
    Mononuclear Cells Mnc Cd45, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc cd45/product/R&D Systems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc cd45 - by Bioz Stars, 2020-09
    85/100 stars
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